Plants expressing cell wall degrading enzymes and expression vectors

ABSTRACT

Vectors for expression of proteins in plants are described. The proteins may be enzymes and the enzymes can be but are not limited to cell wall degrading enzymes. A number of plants designed to express specific cell wall degrading enzymes are provided. The plants may have industrial and/or agricultural applications. Methods and materials for making the expression vectors and for making the plants are provided. Processes for which the plants could be used in industrial and agricultural applications are also provided.

This application is a continuation of U.S. application Ser. No.13/508,280 which was filed on Sep. 20, 2012 as a 35 U.S.C. § 371national phase application of PCT/US10/55746 which was filed on Nov. 5,2010 and claimed the benefit of U.S. provisional application No.61/280,635 filed Nov. 6, 2009 and U.S. provisional application No.61/398,589 filed Jun. 28, 2010. U.S. application Ser. No. 13/508,280 isa continuation-in-part of U.S. application Ser. No. 12/590,444 filedNov. 6, 2009 and issued on Apr. 16, 2013 as U.S. Pat. No. 8,420,387 Allof the above applications are incorporated herein by reference as iffully set forth.

The sequence listing electronically filed with this application titled“Sequence Listing,” which was created on Feb. 17, 2016 and had a size of2,226,296 bytes is incorporated by reference herein as if fully setforth.

FIELD OF INVENTION

The disclosure herein relates to plants expressing cell wall degradingenzymes, vectors, nucleic acids, proteins, related methods, andapplications thereof.

BACKGROUND

Hydrolytic enzymes have important industrial and agriculturalapplications, but their expression and production may be associated withadverse phenotypic effects, depending upon the expression host. Inparticular, expression of cell wall degrading enzymes, such ascellulases, xylanases, ligninases, esterases, peroxidases, and otherhydrolytic enzymes are often associated with detrimental effects ongrowth, physiological, and agronomic performance when expressed inplants. Some of these enzymes may also be poorly expressed in microbialhosts, due to their hydrolytic activity.

SUMMARY

In an aspect, the invention relates to a transgenic plant including anucleic acid encoding an amino acid sequence with at least 90% identityto a sequence selected from SEQ ID NOS: 44-115.

In an aspect, the invention relates to a transgenic plant including afirst nucleic acid that is capable of hybridizing under conditions ofmoderate stringency to a second nucleic acid consisting of a nucleotidesequence selected from SEQ ID NOS: 116-187 or the complement thereof.

In an aspect, the invention relates to a vector including a firstnucleic acid capable of hybridizing under conditions of one of low,moderate or high stringency to a second nucleic acid consisting of thesequence of one of SEQ ID NOS: 116-187.

In an aspect, the invention relates to a vector including a nucleic acidhaving a sequence with at least 90% identity to a reference sequenceselected from SEQ ID NOS: 188-283.

In an aspect, the invention relates to a method of processing plantbiomass. The method includes pretreating a plant or part thereof throughmixing the plant or part thereof with liquid to form a mixture having aliquid to solid ratio of less than or equal to 15. Pretreating alsoincludes providing conditions to maintain the mixture at a temperatureless than or equal to 100° C. The method also includes providing one ormore enzyme for modification of at least one component of the plant orpart thereof.

BRIEF DESCRIPTION OF THE DRAWINGS

The patent or application file contains at least one drawing executed incolor. Copies of this patent or patent application publication withcolor drawing(s) will be provided by the Office upon request and paymentof the necessary fee.

The following detailed description of the preferred embodiment of thepresent invention will be better understood when read in conjunctionwith the appended drawings. For the purpose of illustrating theinvention, there are shown in the drawings embodiments which arepresently preferred. It is understood, however, that the invention isnot limited to the precise arrangements and instrumentalities shown. Inthe drawings:

FIG. 1 illustrates a vector map of pSB11.

FIG. 2A illustrates a vector map of AG1000.

FIG. 2B illustrates a vector map of pAG1001.

FIG. 2C illustrates a vector map of pAG1002.

FIG. 3A illustrates a vector map of pAG1003.

FIG. 3B illustrates a vector map of pAG2000.

FIG. 3C illustrates a vector map of pAG2004.

FIG. 4 illustrates a vector map of pAG2014.

FIG. 5 illustrates a vector map of pBSK:OsUbi3P:XmaI:AvrII:NosT.

FIG. 6 illustrates a vector map of pBSK:OsUbi3P:XmaI:AvrII:NosT:L1.

FIG. 7 illustrates the specific activity of three xylanases withaccession numbers P40942, P77853 and O30700.

FIG. 8 illustrates the activity of various transgenic plant samplesexpressing Xylanase P77853.

FIG. 9 illustrates thermal stability assays for O30700, P77853 andP40942.

FIG. 10 illustrates a process flow diagram for a macro-scale process.

FIG. 11 illustrates a process flow diagram for a micro-scale process.

FIG. 12 illustrates glucose and xylose yields (percentage on biomassweight) from enzymatic hydrolysis of pretreated corn stover (2015.05 and2004.8.4).

FIG. 13 illustrates glucose and xylose yields (percentage on biomassweight) from enzymatic hydrolysis of pretreated corn stover (2004.8.4,2063.13, and 2063.17).

FIG. 14 illustrates glucose and xylose yields (percentage on biomassweight) from enzymatic hydrolysis of pretreated corn stover (2015.05 and2004.8.4).

FIG. 15 illustrates glucose and xylose yields (percentage on biomassweight) from enzymatic hydrolysis of pretreated corn stover (2064.17 and2004.8.4).

FIG. 16 illustrates glucose yield (percentage on biomass weight) fromenzymatic hydrolysis of pretreated corn stover (2042.02, 2042.03,2042.06 and 2004.8.4).

FIG. 17A illustrates a transgenic plant made with pAG3000.

FIG. 17B illustrates a transgenic plant made with pAG3001.

FIG. 18A illustrates a transgenic plant made with pAG2004.

FIG. 18B illustrates a cob from a transgenic plant made with pAG2004.

FIG. 18C illustrates a cob from a transgenic plant made with pAG2004.

FIG. 19A illustrates a transgenic plant made with pAG2005.

FIG. 19B illustrates a transgenic plant made with pAG2005.

FIG. 20 illustrates measurement of reducing sugars from transgenic plantevent #15 transformed with pAG2004.

FIG. 21A illustrates a transgenic plant made with pAG2016.

FIG. 21B illustrates a cob from a transgenic plant made with pAG2016.

FIG. 22 illustrates reducing sugar measurements from transgenic plants.

FIG. 23 illustrates enzyme activity measurements from dried, senescentcorn stover samples.

FIG. 24 illustrates enzyme activity measurements from leaf tissuesamples of transgenic plants made with pAG2015, pAG2014, or pAG2004.

FIG. 25A illustrates a transgenic plant made with pAG2014.

FIG. 25B illustrates a transgenic plant made with pAG2014.

FIG. 25C illustrates a cob from a transgenic plant made with pAG2014.

FIG. 26A illustrates a transgenic plant made with pAG2015.

FIG. 26B illustrates a transgenic plant made with pAG2015.

FIG. 26C illustrates a cob from a transgenic plant made with pAG2015.

FIG. 26D illustrates a cob from a transgenic plant made with pAG2015.

FIG. 27A illustrates a transgenic plant made with pAG2020.

FIG. 27B illustrates a transgenic plant made with pAG2020.

FIG. 27C illustrates a cob from a transgenic plant made with pAG2020.

FIG. 28A illustrates a transgenic plant made with pAG2025.

FIG. 28B illustrates a transgenic plant made with pAG2025.

FIG. 28C illustrates a transgenic plant made with pAG2025.

FIG. 29A illustrates a transgenic plant made with pAG2017.

FIG. 29B illustrates a transgenic plant made with pAG2017.

FIG. 29C illustrates a cob from a transgenic plant made with pAG2017.

FIG. 29D illustrates a cob from a transgenic plant made with pAG2017.

FIG. 30A illustrates a transgenic plant made with pAG2019.

FIG. 30B illustrates a transgenic plant made with pAG2019 in comparisonto a wild type plant.

FIG. 31 illustrates a transgenic plants made with pAG2019 or pAG2027 incomparison to a wild type plant. The left three plants were made withpAG2019. The right three plants were made with pAG2027.

FIG. 32A illustrates two transgenic plants made with pAG2018 on the leftand two non-hydrolase expressing plants on the right.

FIG. 32B illustrates a transgenic plant made with pAG2018.

FIG. 32C illustrates a transgenic plant made with pAG2018.

FIG. 33A illustrates a transgenic plant made with pAG2026.

FIG. 33B illustrates a transgenic plant made with pAG2026.

FIG. 33C illustrates a transgenic plant made with pAG2026.

FIG. 34A illustrates a transgenic plant made with pAG2021.

FIG. 34B illustrates a transgenic plant made with pAG2021.

FIG. 34C illustrates a cob from a transgenic plant made with pAG2021.

FIG. 34D illustrates a cob from a transgenic plant made with pAG2021.

FIG. 35A illustrates a transgenic plant made with pAG2022.

FIG. 35B illustrates a transgenic plant made with pAG2022.

FIG. 35C illustrates a cob from a transgenic plant made with pAG2022.

FIG. 36A illustrates a transgenic plant made with pAG2023.

FIG. 36B illustrates a transgenic plant made with pAG2023.

FIG. 36C illustrates a transgenic plant made with pAG2023.

FIG. 37A illustrates a transgenic plant made with pAG2024.

FIG. 37B illustrates a transgenic plant made with pAG2024.

FIG. 37C illustrates a transgenic plant made with pAG2024.

FIG. 38 illustrates activity data from some of the pAG2021 events, alongwith measurements from pAG2004 events (negative controls for xylanaseactivity) and a pAG20014 event (positive control for xylanase activity).

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

Certain terminology is used in the following description for convenienceonly and is not limiting. The words “right,” “left,” “top” and “bottom”designate directions in the drawings or specific examples to whichreference is made.

The words “a” and “one,” as used in the claims and in the correspondingportions of the specification, are defined as including one or more ofthe referenced item unless specifically stated otherwise. The phrase “atleast one” followed by a list of two or more items, such as “A, B or C,”means any individual one of A, B or C as well as any combinationthereof.

Despite potential detrimental effects of enzymes on the expression host,producing enzymes in plants, microbes, and other organisms can createlarge economic benefits in the production of fuels, fiber, chemicals,sugars, textiles, pulp, paper, and animal feed. In some cases there areeconomic benefits to producing enzymes in plants, despite an agronomicor phenotypic effect. In addition, some phenotypic effects may beovercome using a variety of strategies that protect the plant from theenzyme activity. The embodiments herein include but are not limited tothese strategies.

Strategies for plant-expressed enzymes may be crop dependent. A specificenzyme may have little or no value or benefits when expressed in onecrop, but significant value or benefits when expressed in another crop.That is, the properties of the engineered plant may depend not only onthe specific enzyme, but also on the specific plant that expresses theenzyme. For example, the expression of xylanase enzymes in plants canfacilitate the hydrolysis of plant cell wall hemicellulose, and plantfiber, into fermentable sugars (for the production of fuels andchemicals) or digestible sugars (for animal feed and meat production).However, specific xylanase enzymes also decrease grain yield and maycause infertility when expressed in corn, preventing the use of thatcrop as a host for enzyme expression. Despite the negative effects ongrain yield and fertility in corn, which may decrease the net economicvalue of the engineered plant versus the non-engineered plant,expression of the identical xylanases in another crop, such asswitchgrass, miscanthus, sugarcane or sorghum, may actually bebeneficial because infertility in these crops would prevent theoutcrossing of the xylanase gene and commercially relevant amounts ofplant propagules could be produced using tissue culture or vegetativepropagation. While a decrease in fertility, grain yield, or dry matterbiomass in corn might prevent, or decrease the value of, expression ofspecific xylanase enzymes that would otherwise be valuable in thechemical processing and animal feed industries, expression of theidentical enzymes in switchgrass, miscanthus, sorghum, or sugarcane maynot only provide the economic value created by the enzyme, but could bebeneficial from a regulatory and safety perspective.

Likewise, the value of an enzyme expressed in one tissue of a crop maybe different when expressed in a different tissue, or when expressed inthe same tissue in a different crop. Different benefits result becausespecific crop tissues (such as grain, seed, leaves, stalks, roots,flowers, pollen, etc.) may have different values depending upon the cropand the new properties imparted by the expressed enzyme. Specificxylanase and cellulase enzymes have dramatic agronomic and phenotypiceffects when expressed constitutively in corn. Constitutive expressionof these enzymes, individually or in combination, often results instunted plants, infertile plants, or plants with lower yields andagronomic performance. However, seed specific expression of specificxylanase and cellulase enzymes may decrease or eliminate any detrimentalagronomic effect or yield decrease, while still providing high levels ofenzyme. This may be a benefit in corn grain. Producing the same enzymesin switchgrass, miscanthus, forage or sweet sorghum, or sugarcane, wheregrain yields may be considerably lower on a per acre basis when comparedto corn, may lead to a different profile for seed specific expression ofa xylanase or cellulase. Embodiments include expression of a CWDE seedspecifically in any kind of transgenic plant. Depending upon theapplication, such as animal feed production, meat or dairy production,poultry production, paper production, or the production of fermentablesugars, where the enzyme containing grain could be mixed with otherharvested feedstock (pretreated or unpretreated), this may be a veryeffective way of providing beneficial doses of enzyme in corn grain orother grains and seeds.

The net economic value of a plant-expressed enzyme may differ, dependingupon where the enzyme is designed to localize and accumulate, and whereit is targeted. For example, specific xylanase and cellulase enzymes mayhave dramatic phenotypic and agronomic effects when targeted to theplant cell wall, but little or no effect when maintained intracellularlyor targeted to the vacuole. This may create economic benefits byproviding an intracellularly contained source of enzyme for applicationswhere it is desired to mix the enzyme with a substrate. In contrast,while the same enzymes could provide value in an admix application suchas in animal feed or the processing of pretreated biomass, these enzymesmay provide little or no value in a self-processing application whereplant cell wall targeting is preferred to generate fermentable ordigestible sugars, but problematic because of the resulting phenotypicor agronomic effects.

As described above, an exogenous enzyme can be expressed in a particularplant, plant organ, plant tissue, plant cell, or plant sub-cellularregion or compartment. Embodiments herein include expressing anexogenous enzyme in a plant, a region of a plant, a plant organ, a planttissue, or a sub-cellular plant region or compartment. Embodiments alsoinclude a plant including an exogenous enzyme where the exogenous enzymecan be in the whole plant or localized in a region of the plant, in aplant organ, in plant tissues, or in a plant sub-cellular region orcompartment. Transgenic plants adapted to or having cytoplasmicaccumulation of an exogenous CWDE may be provided. The design of wherein the plant and in what plant the exogenous enzyme is expressed can bebut is not limited to a design that takes into account the phenotypic,safety, economic, or regulatory issues set forth above.

Vectors for expression of proteins in plants are provided in embodimentsherein. The proteins may be enzymes and the enzymes can be but are notlimited to cell wall degrading enzymes. A number of plants designed toexpress specific cell wall degrading enzymes are provided. The plantsmay have industrial and/or agricultural applications. Methods andmaterials for making the expression vectors and for making the plantsare provided. Processes for which the plants could be used in industrialand agricultural applications are also provided.

Vectors for expressing in planta either a cell wall degrading enzyme (orCWDE) or a intein-modified CWDE variant are provided. In an embodiment,the vector is suitable for transformation of a dicotyledonous plant. Inan embodiment, the vector is suitable for transformation of amonocotyledonous plant. The CWDEs from which the CWDE in a vector orplant may be selected from but are not limited to xylanases, cellulases,cellobiohydrolases, glucosidases, xylosidases, arabinofuranosidases, andferulic acid esterases. In an embodiment, the CWDE encoding sequence isdisrupted by the insertion of an intein sequence. The inserted inteinsequence may inactivate the function of the corresponding CWDE. In anembodiment, the vector design permits insertion of at least three tofour gene expression and/or gene silencing cassettes. Each cassettecould include a CWDE or intein-modified CWDE.

In an embodiment, the genetic elements used in a vector herein or in theconstruction thereof can provide at least one of the followingattributes: the ability to select transgenic events after planttransformation, the ability to affect an optimal level of the geneexpression in cells or affect desired sub-cellular enzyme targeting. Thevectors may contain a selectable marker, which can be but is not limitedto a E. coli phosphomannose isomerase (PMI) gene. Other selectablemarkers that can be included, in addition to or in place of the PMImarker, are those known in the art (such as but not limited to EPSPS,BAR, npt-II, GUS, etc). The vectors may also include one or morepromoters. The promoters may be constitutive or global, tissue specific,seed specific, leaf specific, organ specific, sub-cellular region orcompartment specific, or developmental stage specific promoters.Preferred promoters include the rice Ubiquitin 3 gene promoter (OsUbi3P)with the first intron (Accession No. AY954394, SEQ ID NO: 1) or riceActin 1 gene promoter (Accession No. 544221, SEQ ID NO: 2. Otherconstitutive promoters, such as but not limited to the maize ubiquitinpromoter (SEQ ID NO: 3), could also be used and substituted for OsUbi3Por the rice Actin 1 promoter. The Ubiquitin 3 and rice Actin 1 genepromoters are constitutive and global promoters that can be used toprovide gene expression in transgenic plants. The glutelin promoter fromthe rice GluB-4 gene (Accession No. AY427571, SEQ ID NO: 4) with its ownsignal sequence may also be provided in the vectors. The glutelinpromoter is a seed-specific promoter. Other seed specific promoters(such as but not limited to the maize zein Zc2promoter SEQ ID NO: 5)could be provided in the vectors. In order to deliver the enzymes totheir corresponding substrates or to places for high level of enzymeaccumulation such as vacuoles, various targeting signal sequences can beprovided in the vector. Targeting signal sequences that can be providedin a CWDE or vector encoding a CWDE include but are not limited to PR1a(SEQ ID NO: 6, encoded by the nucleic acid sequence of SEQ ID NO: 7),BAASS (SEQ ID NO: 8, encoded by the nucleic acid sequence of SEQ ID NO:9), and barley aleurain (SEQ ID NO: 10, encoded by the nucleic acid ofSEQ ID NO: 11). Other targeting sequences that can be included includebut are not limited to the endoplasmic reticulum (ER) retention sequenceSEKDEL (SEQ ID NO: 12, encoded by the nucleic acid of SEQ ID NO: 13),and the abridged sequence KDEL (SEQ ID NO: 10, encoded by the nucleicacid of SEQ ID NO: 16). The enzymes may be provided without a targetingsequence. The enzymes may be provided such that they accumulate in thecytoplasm. A transcription terminator may be provided. The efficienttranscription terminator sequence from the nopaline synthase gene ofAgrobacterium tumefaciens is used in gene expression cassette examplesherein.

In an embodiment, a transgenic plant including a nucleic acid encoding aCWDE or a CWDE modified with at least one of a signal sequence or anintein is provided. The nucleic acid sequence encoding the CWDE mayencode any CWDE amino acid sequence. The nucleic acid sequence encodingthe CWDE modified with at least one of a signal sequence or an inteinmay encode any CWDE amino acid sequence and at least one of any signalsequence or any intein. The nucleic acid may encode a protein having atleast 70, 72, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100%identity to a sequence selected from SEQ ID NOS: 44-115. The nucleicacid may encode a protein having at least 70, 72, 75, 80, 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to a sequence selectedfrom SEQ ID NOS: 44-45, 49-54, 57-59, 85-86, 94-96, 104-109 and 113-115.The nucleic acid may encode a protein having at least 70, 72, 75, 80,85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to asequence selected from SEQ ID NOS: 47 and 55. The nucleic acid mayencode a protein having at least 70, 72, 75, 80, 85, 90, 91, 92, 93, 94,95, 96, 97, 98, 99 or 100% identity to a sequence selected from SEQ IDNOS: 46, 48 and 56. The nucleic acid may encode a protein having atleast 70, 72, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100%identity to a sequence selected from SEQ ID NOS: 60-67, 70 and 75. Thenucleic acid may encode a protein at having least 70, 72, 75, 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to a sequenceselected from SEQ ID NOS: 68-69, 71-74, 76-77 and 112. The nucleic acidmay encode a protein having at least 70, 72, 75, 80, 85, 90, 91, 92, 93,94, 95, 96, 97, 98, 99 or 100% identity to a sequence selected from SEQID NOS: 78-84. The nucleic acid may encode a protein having at least 70,72, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identityto a sequence selected from SEQ ID NOS: 97-103. The nucleic acid mayencode a protein having at least 70, 72, 75, 80, 85, 90, 91, 92, 93, 94,95, 96, 97, 98, 99 or 100% identity to a sequence selected from SEQ IDNOS: 87-93 and 110-111. The nucleic acid may encode a protein having atleast 70, 72, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100%identity to a sequence selected from SEQ ID NOS: 44, 45, 49 and 54. Thenucleic acid may encode a protein having at least 70, 72, 75, 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to a sequenceselected from SEQ ID NOS: 45, 87, 104-106 and 113. The nucleic acid mayencode a protein having at least 70, 72, 75, 80, 85, 90, 91, 92, 93, 94,95, 96, 97, 98, 99 or 100% identity to a sequence selected from SEQ IDNOS: 50-53, 57-59, 94-96, 104-109 and 113-115. The nucleic acid mayencode a protein having at least 70, 72, 75, 80, 85, 90, 91, 92, 93, 94,95, 96, 97, 98, 99 or 100% identity to a sequence selected from SEQ IDNOS: 54-56 and 60-65. Any of the nucleic acids set for the above thatencode a protein having less than 100% identity to the cited referencesequence may encode a protein having the same or substantially the sameactivity as a protein having 100% identity to the cited referencesequence. Activity may be assessed by assays known in the art for anyparticular protein. Activity may be assessed by a method set forth in anexample or portion thereof herein. Substantially the same activity wouldbe known in the art. In an embodiment, substantially the same activityis within 20% of the activity of a protein having 100% identity to thecited reference sequence. In an embodiment, substantially the sameactivity is within 15% of the activity of a protein having 100% identityto the cited reference sequence. In an embodiment, substantially thesame activity is within 10% of the activity of a protein having 100%identity to the cited reference sequence. In an embodiment,substantially the same activity is within 5% of the activity of aprotein having 100% identity to the cited reference sequence. In anembodiment, substantially the same activity is within 1% of the activityof a protein having 100% identity to the cited reference sequence. Theabove mentioned nucleic acids may be provided in embodiments hereinalone, as part of another nucleic acid, as part of a vector or as statedabove as part of a transgenic plant. Identity can be measured by theSmith-Waterman algorithm (Smith T F, Waterman M S (1981),“Identification of Common Molecular Subsequences,” Journal of MolecularBiology 147: 195-197, which is incorporated by reference in its entiretyas if fully set forth). In an embodiment, the transgenic plant may bederived from one of corn, switchgrass, miscanthus, sugarcane or sorghum.The transgenic plant may be made by agrobacterium mediatedtransformation using a plasmid having a nucleotide sequence as set forthabove. The plasmid have a sequence with at least 70, 72, 75, 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to a sequenceselected from SEQ ID NOS: 188-283. The plasmid consist essentially of asequence with at least 70, 72, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99 or 100% identity to a sequence selected from SEQ ID NOS:188-283. The plasmid consist of a sequence with at least 70, 72, 75, 80,85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to asequence selected from SEQ ID NOS: 188-283.

In an embodiment, a transgenic plant including a nucleic acidhybridizing to a reference nucleic acid encoding a CWDE or a CWDEmodified with at least one of a signal sequence or an intein isprovided. The reference nucleic acid sequence encoding the CWDE mayencode any CWDE amino acid sequence. The reference nucleic acid sequenceencoding the CWDE modified with at least one of a signal sequence or anintein may encode any CWDE amino acid sequence and at least one of anysignal sequence or any intein. The nucleic acid included in thetransgenic plant may be referred to as a first nucleic acid. The firstnucleic acid may be capable of hybridizing under conditions of lowstringency to a second nucleic acid consisting of a nucleotide sequenceselected from SEQ ID NOS: 116-187 or the complement thereof. The firstnucleic acid may be capable of hybridizing under conditions of moderatestringency to a second nucleic acid consisting of a nucleotide sequenceselected from SEQ ID NOS: 116-187 or the complement thereof. The firstnucleic acid may be capable of hybridizing under conditions of highstringency to a second nucleic acid consisting of a nucleotide sequenceselected from SEQ ID NOS: 116-187 or the complement thereof. The firstnucleic acid may be capable of hybridizing under conditions of low,moderate or high stringency to a second nucleic acid consisting of anucleotide sequence selected from SEQ ID NOS: 116-117, 121-126, 129-131,157-158, 166-168, 176-181 and 185-187 or the complement thereof. Thefirst nucleic acid may be capable of hybridizing under conditions oflow, moderate or high stringency to a second nucleic acid consisting ofa nucleotide sequence selected from SEQ ID NOS: 119 and 127 or thecomplement thereof. The first nucleic acid may be capable of hybridizingunder conditions of low, moderate or high stringency to a second nucleicacid consisting of a nucleotide sequence selected from SEQ ID NOS: 118,120 and 128 or the complement thereof. The first nucleic acid may becapable of hybridizing under conditions of low, moderate or highstringency to a second nucleic acid consisting of a nucleotide sequenceselected from SEQ ID NOS: 132-139, 142 and 147 or the complementthereof. The first nucleic acid may be capable of hybridizing underconditions of low, moderate or high stringency to a second nucleic acidconsisting of a nucleotide sequence selected from SEQ ID NOS: 140-141,143-146, 148-149 and 184 or the complement thereof. The first nucleicacid may be capable of hybridizing under conditions of low, moderate orhigh stringency to a second nucleic acid consisting of a nucleotidesequence selected from SEQ ID NOS: 150-156 or the complement thereof.The first nucleic acid may be capable of hybridizing under conditions oflow, moderate or high stringency to a second nucleic acid consisting ofa nucleotide sequence selected from SEQ ID NOS: 169-175 or thecomplement thereof. The first nucleic acid may be capable of hybridizingunder conditions of low, moderate or high stringency to a second nucleicacid consisting of a nucleotide sequence selected from SEQ ID NOS:159-165 and 182-183 or the complement thereof. The first nucleic acidmay be capable of hybridizing under conditions of low, moderate or highstringency to a second nucleic acid consisting of a nucleotide sequenceselected from SEQ ID NOS: 116, 117, 121 and 126 or the complementthereof. The first nucleic acid may be capable of hybridizing underconditions of low, moderate or high stringency to a second nucleic acidconsisting of a nucleotide sequence selected from SEQ ID NOS: 117, 159,176-178 and 185 or the complement thereof. The first nucleic acid may becapable of hybridizing under conditions of low, moderate or highstringency to a second nucleic acid consisting of a nucleotide sequenceselected from SEQ ID NOS: 122-125, 129-131, 166-168, 176-181 and 185-187or the complement thereof. The first nucleic acid may be capable ofhybridizing under conditions of low, moderate or high stringency to asecond nucleic acid consisting of a nucleotide sequence selected fromSEQ ID NOS: 126-128 and 132-137 or the complement thereof. Examples ofhybridization protocols and methods for optimization of hybridizationprotocols are described in the following books: Molecular Cloning, T.Maniatis, E. F. Fritsch, and J. Sambrook, Cold Spring Harbor Laboratory,1982; and, Current Protocols in Molecular Biology, F. M. Ausubel, R.Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, K.Struhl, Volume 1, John Wiley & Sons, 2000, which are incorporated hereinby reference as if fully set forth. By way of example, but notlimitation, procedures for hybridization conditions of moderatestringency are as follows: filters containing DNA are pretreated for 2-4h at 68° C. in a solution containing 6×SSC (Amresco, Inc., Solon, Ohio),0.5% SDS (Amersco, Inc., Solon, Ohio), 5×Denhardt's solution (Amersco,Inc., Solon, Ohio), and 100 μg/mL denatured, salmon sperm DNA(Invitrogen Life Technologies, Inc., Carlsbad, Calif.). Approximately0.2 mL of pretreatment solution are used per square centimeter ofmembrane used. Hybridizations are carried out in the same solution withthe following modifications: 0.01 M EDTA (Amersco, Inc., Solon, Ohio),100 μg/ml salmon sperm DNA, and 5-20×10⁶ cpm ³²P-labeled orfluorescently labeled probes can be used. Filters are incubated inhybridization mixture for 16-20 h at 68° C. and then washed for 15minutes at room temperature (within five degrees of 25° C.) in asolution containing 2×SSC and 0.1% SDS, with gentle agitation. The washsolution is replaced with a solution containing 0.1×SSC and 0.5% SDS,and incubated an additional 2 h at 68° C., with gentle agitation.Filters are blotted dry and exposed for development in an imager or byautoradiography. If necessary, filters are washed for a third time andre-exposed for development. By way of example, but not limitation, lowstringency refers to hybridizing conditions that employ low temperaturefor hybridization, for example, temperatures between 37° C. and 60° C.By way of example, but not limitation, high stringency refers tohybridizing conditions as set forth above but with modification toemploy high temperatures, for example, hybridization temperatures over68° C. Any of the nucleic acids set for the above that have less than100% identity to the cited reference sequence may encode a proteinhaving the same or substantially the same activity as a protein encodedby a nucleic acid sequence having 100% identity to the cited referencesequence. Activity may be assessed by assays known in the art for anyparticular protein. Activity may be assessed by a method set forth in anexample or portion thereof herein. Substantially the same activity wouldbe known in the art. In an embodiment, substantially the same activityis within 20% of the activity of a protein encoded by a nucleic acidsequence having 100% identity to the cited reference sequence. In anembodiment, substantially the same activity is within 15% of theactivity of a protein encoded by a nucleic acid sequence having 100%identity to the cited reference sequence. In an embodiment,substantially the same activity is within 10% of the activity of aprotein encoded by a nucleic acid sequence having 100% identity to thecited reference sequence. In an embodiment, substantially the sameactivity is within 5% of the activity of a protein encoded by a nucleicacid sequence having 100% identity to the cited reference sequence. Inan embodiment, substantially the same activity is within 1% of theactivity of a protein encoded by a nucleic acid sequence having 100%identity to the cited reference sequence. The transgenic plant may bederived from one of corn, switchgrass, miscanthus, sugarcane or sorghum.The transgenic plant may be made by Agrobacterium mediatedtransformation using a plasmid including any of the above nucleic acids.

In an embodiment, a vector including a nucleic acid encoding a CWDE or aCWDE modified with at least one of a signal sequence or an intein isprovided. The nucleic acid sequence encoding the CWDE may encode anyCWDE amino acid sequence. The nucleic acid sequence encoding the CWDEmodified with at least one of a signal sequence or an intein may encodeany CWDE amino acid sequence and at least one of any signal sequence orany intein. The nucleic acid may encode a protein having least 70, 72,75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to asequence selected from SEQ ID NOS: 44-115. The nucleic acid sequence mayhybridize under conditions of low stringency to a reference nucleic acidconsisting of the sequence of one of SEQ ID NOS: 116-187 or thecomplement thereof. The nucleic acid sequence may hybridize underconditions of moderate stringency to a reference nucleic acid consistingof the sequence of one of SEQ ID NOS: 116-187 or the complement thereof.The nucleic acid sequence may hybridize under conditions of highstringency to a reference nucleic acid consisting of the sequence of oneof SEQ ID NOS: 116-187 or the complement thereof. The vector may includea sequence having 70, 72, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99or 100% identity to a sequence selected from SEQ ID NOS: 188-283. Thevector may consist essentially of a sequence having 70, 72, 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99 or 100% identity to a sequenceselected from SEQ ID NOS: 188-283. The vector may consist of a sequencehaving 70, 72, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100%identity to a sequence selected from SEQ ID NOS: 188-283.

In an embodiment, an isolated nucleic acid, polynucleotide, oroligonucleotide encoding at least a portion of any of the amino acidsequences of SEQ ID NOS: 44-115 can be used as a hybridization probe orprimer. In an embodiment, the complement of said isolated nucleic acid,polynucleotide or oligonucleotide may be used as a hybridization probeor primer. In an embodiment, an isolated nucleic acid having a sequencethat hybridizes under conditions of low, moderate or high stringency toat least a portion of a nucleic acid having the sequence of any one ofSEQ ID NOS: 116-187 and 188-283 or the complement thereof may be used asa hybridization probe or primer. These isolated nucleic acids,polynucleotides, or oligonucleotides are not limited to but may have alength in the range from 10 to 100, 10 to 90, 10 to 80, 10 to 70, 10 to60, 10 to 50, 10 to 40, 10 to 35, 10 to 30, 10 to 25, 10 to 20 or 10 to15 nucleotides, or from 20 to 30 nucleotides, or be 25 nucleotides inlength. A range of nucleotide sequence lengths recited herein includesevery length of nucleotide sequence within the range, endopointsinclusive. The recited length of nucleotides may start at any singleposition within a reference sequence where enough nucleotides follow thesingle position to accommodate the recited length. In an embodiment, ahybridization probe or primer is 85 to 100%, 90 to 100%, 91 to 100%, 92to 100%, 93 to 100%, 94 to 100%, 95 to 100%, 96 to 100%, 97 to 100%, 98to 100%, 99 to 100%, or 100% complementary to a nucleic acid with thesame length as the probe or primer and having a sequence chosen from alength of nucleotides corresponding to the probe or primer length withina nucleic acid encoding one of the proteins of SEQ ID NOS: 44-115 or thecomplement of said nucleic acid. In an embodiment, a hybridization probeor primer is 85 to 100%, 90 to 100%, 91 to 100%, 92 to 100%, 93 to 100%,94 to 100%, 95 to 100%, 96 to 100%, 97 to 100%, 98 to 100%, 99 to 100%,or 100% complementary to a nucleic acid with the same length as theprobe or primer and having a sequence chosen from a length ofnucleotides corresponding to the probe or primer length within a nucleicacid with the sequence of one of SEQ ID NOS: 116-283. In an embodiment,a hybridization probe or primer hybridizes along its length to acorresponding length of a nucleic acid encoding the sequence of one ofSEQ ID NOS: 44-115 or the complement said nucleic acid. In anembodiment, a hybridization probe or primer hybridizes along its lengthto a corresponding length of a nucleic acid having the sequence of oneof SEQ ID NOS: 116-187 or the complement thereof. In an embodiment,hybridization can occur under conditions of low stringency. In anembodiment, hybridization can occur under conditions of moderatestringency. In an embodiment, hybridization can occur under conditionsof high stringency.

The isolated nucleic acids, polynucleotides, or oligonucleotides ofembodiments herein may include natural nucleotides, natural nucleotideanalogues, or synthetic nucleotide analogues. Nucleic acids,polynucleotides, or oligonucleotides of embodiments herein may be anykind of nucleic acid including deoxyribonucleic acid (DNA), ribonucleicacid (RNA), or peptide nucleic acid (PNA). The nucleic acid sequenceslisted herein are listed as DNA sequences but other nucleic acids arecontemplated as embodiments herein, including RNA sequences where Ureplaces T.

Although non-labeled hybridization probes or primers can be used in theembodiments herein, the hybridization probes or primers may bedetectably labeled and could be used to detect, sequence, or synthesizenucleic acids. Exemplary labels include, but are not limited to,radionuclides, light-absorbing chemical moieties, dyes, and fluorescentmoieties. The label may be a fluorescent moiety, such as6-carboxyfluorescein (FAM), 6-carboxy-4,7,2′,7′-tetrachlorofluoroscein(TET), rhodamine, JOE (2,7-dimethoxy-4,5-dichloro-6-carboxyfluorescein),HEX (hexachloro-6-carboxyfluorescein), or VIC.

In an embodiment, a method of processing plant biomass is provided. Themethod may include pretreating a plant or part thereof through mixingthe plant or part thereof with liquid to form a mixture having a liquidto solid ratio of less than or equal to 15. Pretreating may includeproviding conditions to maintain the mixture at a temperature less thanor equal to 100° C. The method may include providing one or more enzyme.The plant biomass may be or be derived from any plant or part thereof.The plant biomass may be or be derived from any transgenic plant or partthereof described, illustrated or claimed herein. The method may includea plant or part thereof other than any transgenic plant or part thereofdescribed, illustrated or claimed herein, and combining it with anytransgenic plant or part thereof described, illustrated or claimedherein. The liquid to solid ratio in the mixture may be a value lessthan or equal to 25, 24, 23, 22, 21, 20, 19, 18, 17, 16, 15, 14, 13, 12,11, 10, 9, 8, 7, 6, 5, 4, 3, 2 or 1. The liquid to solid ratio may be 8or less. The liquid to solid ratio may be 8. The step of pretreating mayinclude maintaining the temperature of less than or equal to 100° C. forat least four hours. The step of pretreating may include maintaining thetemperature of 40° C. to 90° C. The liquid provided to make the mixturemay be any liquid. In an embodiment, the liquid is water. In anembodiment, the liquid includes water, ammonium bisulfite and ammoniumcarbonate. The ammonium bisulfite may be at any suitable concentration.In an embodiment, the ammonium bisulfite concentration is a value within8% to 38% (endpoints inclusive) on a wt./wt. basis with the plant orpart thereof. The ammonium carbonate may be at any suitable pH. In anembodiment, the ammonium carbonate pH is a pH in the range of 7.6 to8.5, enpoints inclusive. The ammonium carbonate concentration may be anysuitable concentration. In an embodiment, the ammonium carbonateconcentration is a value within 4% to 19% (endpoints inclusive) on awt./wt. basis with the plant or part thereof. The step of providing oneor more enzyme may include providing any enzyme suitable for processingplant biomass. In an embodiment, the one or more enzyme includes atleast one enzyme capable hydrolyzing lignocellulosic material. In anembodiment, the one or more enzymes include at least one of anendoglucanase, a β-glucosidase, a cellobiohydrolase or a xylanase. In anembodiment, the one or more enzymes include at least one of a xylanase,a cellulase, a cellobiohydrolase, a glucosidase, a xylosidase, anarabinofuronosidase or a ferulic acid esterase. In an embodiment, themethod includes a step of providing one or more enzyme where the one ormore enzyme is not a xylanase, and then adding a xylanase as anadditional step.

Any single embodiment herein may be supplemented with one or moreelement from any one or more other embodiment herein.

EXAMPLES

The following non-limiting examples are provided to illustrateparticular embodiments. The embodiments throughout may be supplementedwith one or more detail from any one or more example below.

Example 1—pSB11

Referring to FIG. 1, a vector of an embodiment herein may be based onthe pSB11 intermediate plasmid (a derivative of pBR322). pSB11 isavailable from Japan Tobacco. The pSB11 plasmid is suitable for cloningand can be easily maintained in E. coli. The pSB11 conjugates with thepSB1 “super-binary” acceptor vector (a disarmed Ti plasmid), which canbe maintained in the LB4404 strain of Agrobacterium tumefaciens, throughhomologous recombination using cos and on sites present in both vectors.The integration product represents a hybrid vector that can besubsequently used for plant transformation. pSB1 contains virulencegenes such as virB, virC and virG required for T-DNA processing anddelivering to the plant cell. pSB11 has a multiple cloning sitecontaining unique restriction enzyme recognition sites for cloningexpression cassettes with the target gene sequences.

Example 2—pAG1000

Referring to FIG. 2A, pAG1000 was created by modification of pSB11 inorder to enable it to accept several gene expression cassettes.Initially, the original expression cassette containing a positiveselectable marker gene manA encoding phosphomannose isomerase (PMI)driven by the Cestrum Yellow Leaf Curling Virus promoter (CMPS) wascloned from pNOV2819 plasmid (Syngenta Biotechnology) into pSB11 asHindIII-KpnI fragment to generate pAG1000.

Example 3—pAG1001, pAG1002 and pAG1003

pAG1000 was further modified by removal of EcoRI site (nucleotideposition #7) to generate pAG1001 (FIG. 2B) and then KpnI site (ntposition #1) to produce pAG1002 (FIG. 2C). These modifications made theEcoRI and KpnI sites available for subsequent cloning expressioncassettes with the genes of interest (GOI). Referring to FIG. 3A, a newmultiple cloning site (MCS) sequence, below, containing PacI, XhoI,SnaBI, NcoI, KpnI, XmaI, AvrII, EcoRI sites, was PCR synthesized as a249 bp PmeI-HindIII fragment and cloned into PmeI-HindIII sites ofpAG1002 to provide the pAG1003 vector.

>MCS (SEQ ID NO: 17) GTTTAAACTGAAGGCGGGAAACGACAACCTGATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCACTCAGCTTAATTAAGTCTAACTCGAGTTACTGGTACGTACCAAATCCATGGAATCAAGGTACCATCAATCCCGGGTATTCATCCTAGGTATCCAAGAATTCATACTAAAGCTT

Example 4—pAG2000

Referring to FIG. 3B, higher expression levels may be provided byreplacing the viral CMPS promoter in pAG1003 by the rice Ubiquitin 3promoter (SEQ ID NO: 1), which is an extensively studied promoter withdemonstrated efficacy for gene expression in monocots. The OsUbi3P hasbeen cloned from the pRESQ101 plasmid. pRESQ101 was described by E.Sivamani, J. D. Starmer, R. Qu, “Sequence analysis of rice rubi3promoter gene expression cassettes for improved transgene expression,”Plant Science, 177(6): 549-556, 2009, which is incorporated herein byreference as if fully set forth. The following modifications were madeto the OsUbi3P for the cloning purposes: 1) An EcoRI site was introducedat the 5′ end via a PCR approach; 2) an XmaI site was removed, while aBamHI site was added to the 3′ end. A partial sequence of OsUbi3P wasassembled as an ApaI-BamHI fragment in pBluescript and then cloned asthe HindIII-BamHI entire promoter region including the first Ubiquitinintron fused to PMI in pAG1003 digested with HindIII-SpeI. The lattercloning produced the pAG2000 vector.

Example 5—pAG2004 and pAG2005

The pAG2000 vector was further modified in order to develop a cloningvector amenable of accepting GOI expression cassettes while providingenhanced expression of the PMI selectable marker for planttransformation. The optimization of PMI expression included replacementof original junction sequence connecting the OsUbi3 intron with thestart PMI gene codon in pAG2000 (shown in SEQ ID NO: 18, below) by a new9 nt sequence. The original junction sequence is underlined and thestart codon is in bold in the version of SEQ ID NO: 18 presented below.The new 9 nt sequence is shown as boxed in the version of SEQ ID NO: 19presented below. The boxed sequence was validated as the efficacioussequence in providing a high level of transient GUS expression inpRESQ48 by E. Sivamani and R. Qu (2006), which is incorporated herein byreference as if fully set forth. This 9 nt sequence represents the threeinitial codons of the rice Ubiquitin 3 gene, where the start codon ATGhas been modified to ATC in order to eliminate an additional translationinitiation site. To achieve this modification, the BglII-XcmI fragmentof pAG2000 (nucleotide positions 9726-105) was replaced by the PCRsynthesized fragment, which contained the required 9 nt junctionsequence and was generated in successive reactions using primersP64/P68, P64/P66, and P64/P67.

>BglII-XcmI (9726-105) of pAG2000 (SEQ ID NO: 18)AgatctgttgtcctgtagttacttatgtcagttttgttattatctgaagatatttttggttgttgcttgttgatgtggtgtgagctgtgagcagcgctcttatgattaatgatgctgtccaattgtagtgtagtatgatgtgattgatatgttcatctattttgagctgacagtaccgatatcgtaggatctggtgccaacttattctccagctgcttttttttacctatgttaattccaatcctttcttgcctcttccagGGATCCCCGATCATGCAAAAACTCATTAACTCAGTGCAAAACTATGCCTGGGGCAGCAAAACGGCGTTGACTGAACTTTATGGTATGGAAAATCCGTCCAGCCAGCCGATGG >BglII-XcmI PCR synthesized fragment for pAG2004 construction(SEQ ID NO: 19)Agatctgttgtcctgtagttacttatgtcagttttgttattatctgaagatatttttggttgttgcttgttgatgtggtgtgagctgtgagcagcgctcttatgattaatgatgctgtccaattgtagtgtagtatgatgtgattgatatgttcatctattttgagctgacagtaccgatatcgt

CAGAAACTCATTAACTCAGTGCAAAACTATGCCTGGGGCAGCAAAACGGCGTTGACTGAACTTTATGGTATGGAAAATCCGTCCAGCCAGCCGATGG

Primer Sequence P64 AGATCTGTTGTCCTGTAGTTACTTATGTCA (SEQ ID NO: 20) P66CCATCGGCTGGCTGGACGGATTTTCCATACCATAAAGTTCAGTC AACGCCGTTTTGCTGCCCCAGGCATA(SEQ ID NO: 21) P67 CCATCGGCTGGCTGGACGGATTTTC (SEQ ID NO: 22) P68CGTTTTGCTGCCCCAGGCATAGTTTTGCACTGAGTTAATGAGTTTCTGCATTATCTGGATCTGGAAGAGGCAAGAAAGGATTGGA (SEQ ID NO: 23)

Referring to FIG. 3C, the modifications above lead the pAG2004 vector,which is an embodiment herein. The pAG2004 vector was subsequently usedto conjugate with pSB1 in LBA4404 strain of Agrobacterium tumefaciensand to transform immature maize embryos using Japan Tobaccotransformation procedure (Japan Tobacco Operating Manual for plasmidpSB1, Version 3.1, Jun. 5, 2006; Komari, T., et. al., “Binary Vectorsand Super-binary Vectors”, Methods in Molecular Biology, Volume 343:Agrobacterium Protocols, pages 15-41, Humana Press, which isincorporated herein by reference as if fully set forth). The maizetransformation efficiencies of the pAG2004 and its derivative pAG2005,which contain OsUbi3 promoter cloned as KpnI-XmaI into pAG2004 MCS, maybe in the range of 20-60%, while the pAG1003 with the original PMIexpression cassette from pNOV2819, where manA expression is driven bythe CMPS viral promoter, may provide up to 15% transformationefficiency.

The sequence of pAG2005 is given in SEQ ID NO: 24, which is set forthbelow:

(SEQ ID NO: 24) aattcatactaaagcttgcatgcctgcaggtcgactctagtaacggccgccagtgtgctggaattaattcggcttgtcgaccacccaaccccatatcgacagaggatgtgaagaacaggtaaatcacgcagaagaacccatctctgatagcagctatcgattagaacaacgaatccatattgggtccgtgggaaatacttactgcacaggaagggggcgatctgacgaggccccgccaccggcctcgacccgaggccgaggccgacgaagcgccggcgagtacggcgccgcggcggcctctgcccgtgccctctgcgcgtgggagggagaggccgcggtggtgggggcgcgcgcgcgcgcgcgcgcagctggtgcggcggcgcgggggtcagccgccgagccggcggcgacggaggagcagggcggcgtggacgcgaacttccgatcggttggtcagagtgcgcgagttgggcttagccaattaggtctcaacaatctattgggccgtaaaattcatgggccctggtttgtctaggcccaatatcccgttcatttcagcccacaaatatttccccagaggattattaaggcccacacgcagcttatagcagatcaagtacgatgtttcctgatcgttggatcggaaacgtacggtcttgatcaggcatgccgacttcgtcaaagagaggcggcatgacctgacgcggagttggttccgggcaccgtctggatggtcgtaccgggaccggacacgtgtcgcgcctccaactacatggacacgtgtggtgctgccattgggccgtacgcgtggcggtgaccgcaccggatgctgcctcgcaccgccttgcccacgctttatatagagaggttttctctccattaatcgcatagcgagtcgaatcgaccgaaggggagggggagcgaagctttgcgttctctaatcgcctcgtcaaggtaactaatcaatcacctcgtcctaatcctcgaatctctcgtggtgcccgtctaatctcgcgattttgatgctcgtggtggaaagcgtaggaggatcccgtgcgagttagtctcaatctctcagggtttcgtgcgattttagggtgatccacctcttaatcgagttacggtttcgtgcgattttagggtaatcctcttaatctctcattgatttagggtttcgtgagaatcgaggtagggatctgtgttatttatatcgatctaatagatggattggttttgagattgttctgtcagatggggattgtttcgatatattaccctaatgatgtgtcagatggggattgtttcgatatattaccctaatgatgtgtcagatggggattgtttcgatatattaccctaatgatggataataagagtagttcacagttatgttttgatcctgccacatagtttgagttttgtgatcagatttagttttacttatttgtgcttagttcggatgggattgttctgatattgttccaatagatgaatagctcgttaggttaaaatctttaggttgagttaggcgacacatagtttatttcctctggatttggattggaattgtgttcttagtttttttcccctggatttggattggaattgtgtggagctgggttagagaattacatctgtatcgtgtacacctacttgaactgtagagcttgggttctaaggtcaatttaatctgtattgtatctggctctttgcctagttgaactgtagtgctgatgttgtactgtgtttttttacccgttttatttgctttactcgtgcaaatcaaatctgtcagatgctagaactaggtggctttattctgtgttcttacatagatctgttgtcctgtagttacttatgtcagttttgttattatctgaagatatttttggttgttgcttgttgatgtggtgtgagctgtgagcagcgctcttatgattaatgatgctgtccaattgtagtgtagtatgatgtgattgatatgttcatctattttgagctgacagtaccgatatcgtaggatctggtgccaacttattctccagctgcttttttttacctatgttaattccaatcctttcttgcctctccagatccagataatgcagaaactcattaactcagtgcaaaactatgcctggggcagcaaaacggcgttgactgaactttatggtatggaaaatccgtccagccagccgatggccgagctgtggatgggcgcacatccgaaaagcagttcacgagtgcagaatgccgccggagatatcgtttcactgcgtgatgtgattgagagtgataaatcgactctgctcggagaggccgttgccaaacgctttggcgaactgcctttcctgttcaaagtattatgcgcagcacagccactctccattcaggttcatccaaacaaacacaattctgaaatcggttttgccaaagaaaatgccgcaggtatcccgatggatgccgccgagcgtaactataaagatcctaaccacaagccggagctggtttttgcgctgacgcctttccttgcgatgaacgcgtttcgtgaattttccgagattgtctccctactccagccggtcgcaggtgcacatccggcgattgctcactttttacaacagcctgatgccgaacgtttaagcgaactgttcgccagcctgttgaatatgcagggtgaagaaaaatcccgcgcgctggcgattttaaaatcggccctcgatagccagcagggtgaaccgtggcaaacgattcgtttaatttctgaattttacccggaagacagcggtctgttctccccgctattgctgaatgtggtgaaattgaaccctggcgaagcgatgttcctgttcgctgaaacaccgcacgcttacctgcaaggcgtggcgctggaagtgatggcaaactccgataacgtgctgcgtgcgggtctgacgcctaaatacattgatattccggaactggttgccaatgtgaaattcgaagccaaaccggctaaccagttgttgacccagccggtgaaacaaggtgcagaactggacttcccgattccagtggatgattttgccttctcgctgcatgaccttagtgataaagaaaccaccattagccagcagagtgccgccattttgttctgcgtcgaaggcgatgcaacgttgtggaaaggttctcagcagttacagcttaaaccgggtgaatcagcgtttattgccgccaacgaatcaccggtgactgtcaaaggccacggccgtttagcgcgtgtttacaacaagctgtaagagcttactgaaaaaattaacatctcttgctaagctgggagctctagatccccgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattggcgagctcgaattaattcagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaatttgtttacaccacaatatatcctgccaccagccagccaacagctccccgaccggcagctcggcacaaaatcaccactcgatacaggcagcccatcagtccgggacggcgtcagcgggagagccgttgtaaggcggcagactttgctcatgttaccgatgctattcggaagaacggcaactaagctgccgggtttgaaacacggatgatctcgcggagggtagcatgttgattgtaacgatgacagagcgttgctgcctgtgatcaaatatcatctccctcgcagagatccgaattatcagccttcttattcatttctcgcttaaccgtgacaggctgtcgatcttgagaactatgccgacataataggaaatcgctggataaagccgctgaggaagctgagtggcgctatttctttagaagtgaacgttgacgatcgtcgaccgtaccccgatgaattaattcggacgtacgttctgaacacagctggatacttacttgggcgattgtcatacatgacatcaacaatgtacccgtttgtgtaaccgtctcttggaggttcgtatgacactagtggttcccctcagcttgcgactagatgttgaggcctaacattttattagagagcaggctagttgcttagatacatgatcttcaggccgttatctgtcagggcaagcgaaaattggccatttatgacgaccaatgccccgcagaagctcccatctttgccgccatagacgccgcgccccccttttggggtgtagaacatccttttgccagatgtggaaaagaagttcgttgtcccattgttggcaatgacgtagtagccggcgaaagtgcgagacccatttgcgctatatataagcctacgatttccgttgcgactattgtcgtaattggatgaactattatcgtagttgctctcagagttgtcgtaatttgatggactattgtcgtaattgcttatggagttgtcgtagttgcttggagaaatgtcgtagttggatggggagtagtcatagggaagacgagcttcatccactaaaacaattggcaggtcagcaagtgcctgccccgatgccatcgcaagtacgaggcttagaaccaccttcaacagatcgcgcatagtcttccccagctctctaacgcttgagttaagccgcgccgcgaagcggcgtcggcttgaacgaattgttagacattatttgccgactaccttggtgatctcgcctttcacgtagtgaacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttgtccaagataagcctgcctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgatcgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttccctcatgatgtttaactcctgaattaagccgcgccgcgaagcggtgtcggcttgaatgaattgttaggcgtcatcctgtgctcccgagaaccagtaccagtacatcgctgtttcgttcgagacttgaggtctagttttatacgtgaacaggtcaatgccgccgagagtaaagccacattttgcgtacaaattgcaggcaggtacattgttcgtttgtgtctctaatcgtatgccaaggagctgtctgcttagtgcccactttttcgcaaattcgatgagactgtgcgcgactcctttgcctcggtgcgtgtgcgacacaacaatgtgttcgatagaggctagatcgttccatgttgagttgagttcaatcttcccgacaagctcttggtcgatgaatgcgccatagcaagcagagtcttcatcagagtcatcatccgagatgtaatccttccggtaggggctcacacttctggtagatagttcaaagccttggtcggataggtgcacatcgaacacttcacgaacaatgaaatggttctcagcatccaatgtttccgccacctgctcagggatcaccgaaatcttcatatgacgcctaacgcctggcacagcggatcgcaaacctggcgcggcttttggcacaaaaggcgtgacaggtttgcgaatccgttgctgccacttgttaacccttttgccagatttggtaactataatttatgttagaggcgaagtcttgggtaaaaactggcctaaaattgctggggatttcaggaaagtaaacatcaccttccggctcgatgtctattgtagatatatgtagtgtatctacttgatcgggggatctgctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggggggggggggggggggttccattgttcattccacggacaaaaacagagaaaggaaacgacagaggccaaaaagctcgctttcagcacctgtcgtttcctttcttttcagagggtattttaaataaaaacattaagttatgacgaagaagaacggaaacgccttaaaccggaaaattttcataaatagcgaaaacccgcgaggtcgccgccccgtaacctgtcggatcaccggaaaggacccgtaaagtgataatgattatcatctacatatcacaacgtgcgtggaggccatcaaaccacgtcaaataatcaattatgacgcaggtatcgtattaattgatctgcatcaacttaacgtaaaaacaacttcagacaatacaaatcagcgacactgaatacggggcaacctcatgtccccccccccccccccctgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaattggtcgacgatcttgctgcgttcggatattttcgtggagttcccgccacagacccggattgaaggcgagatccagcaactcgcgccagatcatcctgtgacggaactttggcgcgtgatgactggccaggacgtcggccgaaagagcgacaagcagatcacgcttttcgacagcgtcggatttgcgatcgaggatttttcggcgctgcgctacgtccgcgaccgcgttgagggatcaagccacagcagcccactcgaccttctagccgacccagacgagccaagggatctttttggaatgctgctccgtcgtcaggctttccgacgtttgggtggttgaacagaagtcattatcgcacggaatgccaagcactcccgaggggaaccctgtggttggcatgcacatacaaatggacgaacggataaaccttttcacgcccttttaaatatccgattattctaataaacgctctttctcttaggtttacccgccaatatatcctgtcaaacactgatagtttaaactgaaggcgggaaacgacaacctgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccgttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagcttaattaagtctaactcgagttactggtacgtaccaaatccatggaatcaaggtaccgtcgactctagtaacggccgccagtgtgctggaattaattcggcttgtcgaccacccaaccccatatcgacagaggatgtgaagaacaggtaaatcacgcagaagaacccatctctgatagcagctatcgattagaacaacgaatccatattgggtccgtgggaaatacttactgcacaggaagggggcgatctgacgaggccccgccaccggcctcgacccgaggccgaggccgacgaagcgccggcgagtacggcgccgcggcggcctctgcccgtgccctctgcgcgtgggagggagaggccgcggtggtgggggcgcgcgcgcgcgcgcgcgcagctggtgcggcggcgcgggggtcagccgccgagccggcggcgacggaggagcagggcggcgtggacgcgaacttccgatcggttggtcagagtgcgcgagttgggcttagccaattaggtctcaacaatctattgggccgtaaaattcatgggccctggtttgtctaggcccaatatcccgttcatttcagcccacaaatatttccccagaggattattaaggcccacacgcagcttatagcagatcaagtacgatgtttcctgatcgttggatcggaaacgtacggtcttgatcaggcatgccgacttcgtcaaagagaggcggcatgacctgacgcggagttggttccgggcaccgtctggatggtcgtaccgggaccggacacgtgtcgcgcctccaactacatggacacgtgtggtgctgccattgggccgtacgcgtggcggtgaccgcaccggatgctgcctcgcaccgccttgcccacgctttatatagagaggttttctctccattaatcgcatagcgagtcgaatcgaccgaaggggagggggagcgaagctttgcgttctctaatcgcctcgtcaaggtaactaatcaatcacctcgtcctaatcctcgaatctctcgtggtgcccgtctaatctcgcgattttgatgctcgtggtggaaagcgtaggaggatcccgtgcgagttagtctcaatctctcagggtttcgtgcgattttagggtgatccacctcttaatcgagttacggtttcgtgcgattttagggtaatcctcttaatctctcattgatttagggtttcgtgagaatcgaggtagggatctgtgttatttatatcgatctaatagatggattggttttgagattgttctgtcagatggggattgtttcgatatattaccctaatgatgtgtcagatggggattgtttcgatatattaccctaatgatgtgtcagatggggattgtttcgatatattaccctaatgatggataataagagtagttcacagttatgttttgatcctgccacatagtttgagttttgtgatcagatttagttttacttatttgtgcttagttcggatgggattgttctgatattgttccaatagatgaatagctcgttaggttaaaatctttaggttgagttaggcgacacatagtttatttcctctggatttggattggaattgtgttcttagtttttttcccctggatttggattggaattgtgtggagctgggttagagaattacatctgtatcgtgtacacctacttgaactgtagagcttgggttctaaggtcaatttaatctgtattgtatctggctctttgcctagttgaactgtagtgctgatgttgtactgtgtttttttacccgttttatttgcttactcgtgcaaatcaaatctgtcagatgctagaactaggtggctttattctgtgttcttacatagatctgttgtcctgtagttacttatgtcagttttgttattatctgaagatatttttggttgttgcttgttgatgtggtgtgagctgtgagcagcgctcttatgattaatgatgctgtccaattgtagtgtagtatgatgtgattgatatgttcatctattttgagctgacagtaccgatatcgtaggatctggtgccaacttattctccagctgcttttttttacctatgttaattccaatcctttcttgcctctccagcccgggtattcatcctaggtccccgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgg gaattgg

Example 5—Genetic Elements Used in Vector Development

Promoters

Vectors were made to include a 2014 bp sequence of rice Ubiquitin 3 genepromoter with the first intron (OsUbi3P, Accession # AY954394, SEQ IDNO: 1, shown below) for constitutive or “global” gene expression. Thefirst intron sequence of OsUbi3P is shown as lower case letters in thepresentation of SEQ ID NO: 1 below. Vectors herein can include differentor additional promoters. Vectors were made including the rice Actin1gene promoter with the first gene intron (OsAct1P, Accession No. 544221,SEQ ID NO: 2), which is a constitutive promoter. The rice Actin1 genepromoter may be utilized for PMI gene expression in vectors herein. Forexample, vectors pAG3000-pAG3003 include the rice Actin1 gene promoterwith the first gene intron. Some vectors were made to include the 1474bp rice Glutelin B-4 gene promoter (OsGluB4P, Accession # AY427571, SEQID NO: 4), which may be used for the seed specific gene expression andhas been used to express enzymes and intein-modified enzymes.

>OsUbi3P (SEQ ID NO: 1)CCACCCAACCCCATATCGACAGAGGATGTGAAGAACAGGTAAATCACGCAGAAGAACCCATCTCTGATAGCAGCTATCGATTAGAACAACGAATCCATATTGGGTCCGTGGGAAATACTTACTGCACAGGAAGGGGGCGATCTGACGAGGCCCCGCCACCGGCCTCGACCCGAGGCCGAGGCCGACGAAGCGCCGGCGAGTACGGCGCCGCGGCGGCCTCTGCCCGTGCCCTCTGCGCGTGGGAGGGAGAGGCCGCGGTGGTGGGGGCGCGCGCGCGCGCGCGCGCAGCTGGTGCGGCGGCGCGGGGGTCAGCCGCCGAGCCGGCGGCGACGGAGGAGCAGGGCGGCGTGGACGCGAACTTCCGATCGGTTGGTCAGAGTGCGCGAGTTGGGCTTAGCCAATTAGGTCTCAACAATCTATTGGGCCGTAAAATTCATGGGCCCTGGTTTGTCTAGGCCCAATATCCCGTTCATTTCAGCCCACAAATATTTCCCCAGAGGATTATTAAGGCCCACACGCAGCTTATAGCAGATCAAGTACGATGTTTCCTGATCGTTGGATCGGAAACGTACGGTCTTGATCAGGCATGCCGACTTCGTCAAAGAGAGGCGGCATGACCTGACGCGGAGTTGGTTCCGGGCACCGTCTGGATGGTCGTACCGGGACCGGACACGTGTCGCGCCTCCAACTACATGGACACGTGTGGTGCTGCCATTGGGCCGTACGCGTGGCGGTGACCGCACCGGATGCTGCCTCGCACCGCCTTGCCCACGCTTTATATAGAGAGGTTTTCTCTCCATTAATCGCATAGCGAGTCGAATCGACCGAAGGGGAGGGGGAGCGAAGCTTTGCGTTCTCTAATCGCCTCGTCAAGgtaactaatcaatcacctcgtcctaatcctcgaatctctcgtggtgcccgtctaatctcgcgattttgatgctcgtggtggaaagcgtaggaggatcccgtgcgagttagtctcaatctctcagggtttcgtgcgattttagggtgatccacctcttaatcgagttacggtttcgtgcgattttagggtaatcctcttaatctctcattgatttagggtttcgtgagaatcgaggtagggatctgtgttatttatatcgatctaatagatggattggttttgagattgttctgtcagatggggattgtttcgatatattaccctaatgatgtgtcagatggggattgtttcgatatattaccctaatgatgtgtcagatggggattgtttcgatatattaccctaatgatggataataagagtagttcacagttatgttttgatcctgccacatagtttgagttttgtgatcagatttagttttacttatttgtgcttagttcggatgggattgttctgatattgttccaatagatgaatagctcgttaggttaaaatctttaggttgagttaggcgacacatagtttatttcctctggatttggattggaattgtgttcttagttatttcccctggatttggattggaattgtgtggagctgggttagagaattacatctgtatcgtgtacacctacttgaactgtagagcttgggttctaaggtcaatttaatctgtattgtatctggctctttgcctagttgaactgtagtgctgatgttgtactgtgtttttttacccgttttatttgctttactcgtgcaaatcaaatctgtcagatgctagaactaggtggctttattctgtgttcttacatagatctgttgtcctgtagttacttatgtcagttttgttattatctgaagatatttttggttgttgcttgttgatgtggtgtgagctgtgagcagcgctcttatgattaatgatgctgtccaattgtagtgtagtatgatgtgattgatatgttcatctattttgagctgacagtaccgatatcgtaggatctggtgccaacttattctccagctgcttttttttacctatgttaattccaatcctttc ttgcctcttccag,promoter sequence in upper case letters (SEQ ID NO: 25), first intron inlower case letters (SEQ ID NO: 26) >OsAct1P (SEQ ID NO: 2)TAGCTAGCATATTCGAGGTCATTCATATGCTTGAGAAGAGAGTCGGGATAGTCCAAAATAAAACAAAGGTAAGATTACCTGGTCAAAAGTGAAAACATCAGTTAAAAGGTGGTATAAGTAAAATATCGGTAATAAAAGGTGGCCCAAAGTGAAATTTACTCTTTTCTACTATTATAAAAATTGAGGATGTTTTGTCGGTACTTTGATACGTCATTTTTGTATGAATTGGTTTTTAAGTTTATTCGCGATTTGGAAATGCATATCTGTATTTGAGTCGGTTTTTAAGTTCGTTGCTTTTGTAAATACAGAGGGATTTGTATAAGAAATATCTTTAAAAAACCCATATGCTAATTTGACATAATTTTTGAGAAAAATATATATTCAGGCCAATTCCACAATGAACAATAATAAGATTAAAATAGCTTGCCCCCGTTGCAGCGATGGGTATTTTTTCTAGTAAAATAAAAGATAAACTTAGACTCAAAACATTTACAAAAACAACCCCTAAAGTCCTAAAGCCCAAAGTGCTATGCACGATCCATAGCAAGCCCAGCCCAACCCAACCCAACCCAACCCACCCCAGTGCAGCCAACTGGCAAATAGTCTCCACCCCCGGCACTATCACCGTGAGTTGTCCGCACCACCGCACGTCTCGCAGCCAAAAAAAAAAAAAGAAAGAAAAAAAAGAAAAAGAAAAACAGCAGGTGGGTCCGGGTCGTGGGGGCCGGAAAAGCGAGGAGGATCGCGAGCAGCGACGAGGCCCGGCCCTCCCTCCGCTTCCAAAGAAACGCCCCCCATCGCCACTATATACATACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACCACCACCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTCCTCCCCCCTCCCCCTCCGCCGCCGCCGGTAACCACCCCGCCCCTCTCCTCTTTCTTTCTCCGTTTTTTTTTTCGTCTCGGTCTCGATCTTTGGCCTTGGTAGTTTGGGTGGGCGAGAGCGGCTTCGTCGCCCAGATCGGTGCGCGGGAGGGGCGGGATCTCGCGGCTGGCGTCTCCGGGCGTGAGTCGGCCCGCATCCTCGCGGGGAATGGGGCTCTCGGATGTAGATCTTCTTTCTTTCTTCTTTTTGTGGTAGAATTTGAATCCCTCAGCATTGTTCATCGGTAGTTTTTCTTTTCATGATTTGTGACAAATGCAGCCTCGTGCGGAGCTTTTTTGTAG >OsGluB4P (SEQ ID NO: 4)TACAGGGTTCCTTGCGTGAAGAAGGGTGGCCTGCGGTTCACCATTAACGGTCACGACTACTTCCAGCTAGTACTGGTGACCAACGTCGCGGCGGCAGGGTCAATCAAGTCCATGGAGGTTATGGGTTCCAACACAGCGGATTGGATGCCGATGGCACGTAACTGGGGCGCCCAATGGCACTCACTGGCCTACCTCACCGGTCAAGGTCTATCCTTTAGGGTCACCAACACAGATGACCAAACGCTCGTCTTCACCAACGTCGTGCCACCAGGATGGAAGTTTGGCCAGACATTTGCAAGCAAGCTGCAGTTCAAGTGAGAGGAGAAGCCTGAATTGATACCGGAGCGTTTCTTTTGGGAGTAACATCTCTGGTTGCCTAGCAAACATATGATTGTATATAAGTTTCGTTGTGCGTTTATTCTTTCGGTGTGTAAAATAACATACATGCTTTCCTGATATTTTCTTGTATATATGTACACACACACGACAAATCCTTCCATTTCTATTATTATTGAACAATTTAATTGCGAGGGCGAGTACTTGTCTGTTTACCTTTTTTTTTTCAGATGGCATTTTATAGTTTAACCTTTCATGGACCGGCAGTAGTTCTAACCATGAATGAAAAGAAATCATAGTCCACACCACGCAGGGACATTGTGGTCATTTTAGACAAGACGATTTGATTAATGTCTTGTATGATATGGTCGACAGTGAGGACTAACAAACATATGGCATATTTTATTACCGGCGAGTTAAATAAATTTATGTCACAGTAATAAACTGCCTAATAAATGCACGCCAGAAAATATAATGATAAAAAAAAGAAAAGATACATAAGTCCATTGCTTCTACTTTTTTAAAAATTAAATCCAACATTTTCTATTTTTTGGTATAAACTTGGAAGTACTAGTTGGATATGCAAAATCATCTAACCTCCATATATTTCATCAATTTGTTTACTTTACATATGGGAGAGGATAGTATGTCAAAGAAAATGACAACAAGCTTACAAGTTTCTTATTTTAAAAGTTCCGCTAACTTATCAAGCATAGTGTGCCACGCAAAACTGACAACAAACCAACAAATTTAAGGAGCGCCTAACTTATCATCTATGACATACCGCACAAAATGATAACATACTAGAGAAACTTTATTGCACAAAAGGAAATTTATCCATAAGGCAAAGGAACATCTTAAGGCTTTGGATATACATTTACCAACAAGCATTGTTTGTATTACCCCTAAAGCGCAAGACATGTCATCCATGAGTCATAGTGTGTATATCTCAACATTGCAAAGCTACCTTTTTTCTATTATACTTTTCGCATTATAGGCTAGATATTATCTATACATGTCAACAAACTCTATCCCTACGTCATATCTGAAGATTCTTTTCTTCACTATATAAGTTGGCTTCCCTGTCATTGAACTCACATCAACCAGCCCAAGTTTCCAATAACATCCTCAAATAGCT

The rice Ubiquitin 3 gene promoter was cloned from the pRESQ101, as itis described above, while the rice Act1 and GluB-4 gene promoters weresynthesized. With rice Act1 gene promoter is fused to PMI selectablemarker, up to 23% transformation efficiency was observed in stabletransformation of maize using mannose selection medium during planttissue culture.

Signal Sequences

Signal sequences can be included with a CWDE sequence (with or withoutfurther modification; e.g., with an intein) or in a vector to directenzymes expressed in planta to specific locations within, or externalto, the plant cell. In some examples, described below, the tobacco PR1a(amyloplast targeting) and barley alpha amylase BAASS [SEQ ID NO: 8](cell wall targeting) signal sequences were included in CWDEs or vectorsherein. These signal sequences can direct enzymes to their respectivetargeting locations. In some examples, described below, the barleyaleurain HvAleSP (vacuole targeting), rice GluB4 (seed expression) andER retaining (SEKDEL) signal sequences were included, and thesesequences can localize protein to the respective cellular compartmentsor specific tissues. A goal of such targeting may be achieving highlevels of protein accumulation while avoiding potential detrimentaleffects on plant growth and development. Signal sequences used inexamples herein and their corresponding encoding nucleotide sequencesare presented below:

PR1a protein sequence (SEQ ID NO: 6) M G F V L F S Q L P S F L L V S T LL L F L V I S H S C R A PR1a nucleotide sequence (SEQ ID NO: 7)ATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCTCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCC BAASS protein sequence (SEQ IDNO: 8) M A N K H L S L S L F L V L L G L S A S L A S G Q V BAASSnucleotide sequence (SEQ ID NO: 9)ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTGGCCTGTCGGCCAGCTTGGCCTCCGGGCAAGTC HvAle protein sequence (SEQ ID NO: 10) M AH A R V L L L A L A V L A T A A V A V A S S S S F A D S N P I R P V T DR A A S T HvAle nucleotide sequence (SEQ ID NO: 11)ATGGCCCACGCCCGCGTCCTCCTCCTGGCGCTCGCCGTCCTGGCCACCGCCGCCGTCGCCGTCGCCTCCTCCTCCTCCTTCGCCGACTCCAACCCGATCCGCCCGGTGACCGACCGCGCCGCCTCCACC (SEQ ID NO: 12) SEKDEL (SEQ ID NO: 13)AGCGAGAAGGACGAGCTG (SEQ ID NO: 14) KDEL (SEQ ID NO: 15) AAGGACGAGCTGGluB4SP protein sequence (SEQ ID NO: 27) M A T I A F S R L S I Y F C V LL L C H G S M A GluB4SP nucleotide sequence (SEQ ID NO: 28)ATGGCCACCATCGCTTTCTCCCGCTTGTCCATCTACTTCTGCGTGCTTCTCCTGTGCCACGGCTCCATGGCC

Targeting sequences can be modified from their original versions toreflect the codon usage frequencies for optimal gene expression inmonocot plants. In an embodiment, the host codon usage frequencies arefrom maize. Each signal sequence can be synthesized by PCR usingspecific primers and connected to the 3′ ends of a sequence; forexample, either the OsUbi3 or OsGluB4 promoter, using a fusion PCRapproach.

Transcription Terminator

A transcription terminator can be included in the vectors herein. In anembodiment, the efficient transcription terminator sequence (NosT) fromthe nopaline synthase gene of Agrobacterium tumefaciens is used in geneexpression cassettes cloned in plant transformation vectors. Thesequence is presented below:

NosT (SEQ ID NO: 29) TCCCCGAATTTCCCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGGGAATTGThis sequence appears twice in pAG2005 (SEQ ID NO: 24). The secondappearance at positions 12034 to 12288 follows the second OsUbi3promoter plus intron sequence and XmaI site, and is followed with anEcoRI restriction site (GAATTC, positions 12310 to 5 of SEQ ID NO: 24).The Nos terminator sequence can be PCR amplified from pNOV2819 as 276 bpfragment. Other transcription terminators known in the art could besubstituted and used in place of the Nos terminator. One otherterminator that could be used in place of the Nos terminator is the 35Sterminator.

Example 6—Vector Development for Overexpression of Wild Type P77853Xylanase

Referring to FIG. 4, vector pAG2014 construction provides and example ofa representative approach to clone genes encoding CWDEs such asxylanases, cellulases and any other genes of specific interest fordevelopment of transgenic monocotyledonous plants including, but notlimited to maize, switchgrass, sorghum, miscanthus and sugarcane.

Connection of Signal Sequence to a Coding Region of Mature Enzyme

A signal sequence protein of interest junction can be determinedexperimentally or through models. For this example, the SignalP 3.0server publically available through the Center for Biological SequenceAnalysis of the Technical University of Denmark was used to predict thebest junction between the signal peptide and the wild type P77853xylanase enzyme. The method utilized in SignalP 3.0 incorporates aprediction of cleavage sites and a signal peptide/non-signal peptideprediction based on a combination of several artificial neural networksand hidden Markov models. The program output provides a confidence scorefor the cleavage of signal peptide from the mature protein. Threevariant junctions were evaluated; a first with a direct connectionbetween BAASS [SEQ ID NO: 8] and P77853 ( . . . GQV QTS . . . ), asecond with removal of one amino acid from the carboxy terminus of BAASS( . . . GQ QTS . . . ), and a third with the removal of one amino acidfrom the carboxy terminus of BAASS [SEQ ID NO: 8] and removal of oneamino acid from the amino terminus of P77853 ( . . . GQ TS . . . ). Thevariant with the highest score was advanced to molecular cloning. Thesequences of BASS, P77853, and the first, second and third junctions arebelow with the junction underlined:

BAASS from barley alpha amylase (Ace. #X15226) 78 bp

(SEQ ID NO: 8) M A N K H L S L S L F L V L L G L S A S L A S G Q V (SEQID NO: 9) ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTGGCCTGTCGGCCAGCTTGGCCTCCGGGCAAGTC//

(SEQ ID NO: 30) QTSITLTSNASGTFDGYYYELWKDTGNTTMTVYTQGRFSCQWSNINNALFRTGKKYNQNWQSLGTIRITYSATYNPNGNSYLCIYGWSTNPLVEFYIVESWGNWRPPGATSLGQVTIDGGTYDIYRTTRVNQPSIVGTATFDQYWSVRTSKRTSGTVTVTDHFRAWANRGLNLGTIDQITLCVEGYQSSGSANITQNTFSQGSSSGSSGGSSGSTTTTRIECENMSLSGPYVSRITNPFNGIALYANGDTARATVNFPASRNYNFRLRGCGNNNNLARVDLRIDGRTVGTFYYQGTYPWEAPIDNVYVSAGSHTVEITVTADNGTWDVYADYLVIQBAASS:P77853 1^(st) connection variant

(SEQ ID NO: 31) MANKHLSLSLFLVLLGLSASLASGQVQTSITLTSNASGTFDGYYYELWKDTGNTTMTVYTQGRFSCQWSNINNALFRTGKKYNQNWQSLGTIRITYSATYNPNGNSYLCIYGWSTNPLVEFYIVESWGNWRPPGATSLGQVTIDGGTYDIYRTTRVNQPSIVGTATFDQYWSVRTSKRTSGTVTVTDHFRAWANRGLNLGTIDQITLCVEGYQSSGSANITQNTFSQGSSSGSSGGSSGSTTTTRIECENMSLSGPYVSRITNPFNGIALYANGDTARATVNFPASRNYNFRLRGCGNNNNLARVDLRIDGRTVGTFYYQGTYPWEAPIDNVYVSAGSHTVEITVTADNG TWDVYADYLVIQSignalP3.0 Server Prediction: Signal peptideMost likely cleavage site between pos. 24 and 25: ASG-QVSignal peptide probability: 1.000Max cleavage site probability: 0.740 between pos. 24 and 25BAASS:P77853 2^(nd) connection variant

(SEQ ID NO: 32) MANKHLSLSLFLVLLGLSASLASGQQTSITLTSNASGTFDGYYYELWKDTGNTTMTVYTQGRFSCQWSNINNALFRTGKKYNQNWQSLGTIRITYSATYNPNGNSYLCIYGWSTNPLVEFYIVESWGNWRPPGATSLGQVTIDGGTYDIYRTTRVNQPSIVGTATFDQYWSVRTSKRTSGTVTVTDHFRAWANRGLNLGTIDQITLCVEGYQSSGSANITQNTFSQGSSSGSSGGSSGSTTTTRIECENMSLSGPYVSRITNPFNGIALYANGDTARATVNFPASRNYNFRLRGCGNNNNLARVDLRIDGRTVGTFYYQGTYPWEAPIDNVYVSAGSHTVEITVTADNGT WDVYADYLVIQSignalP3.0 Server Prediction: Signal peptideMost likely cleavage site between pos. 24 and 25: ASG-QQSignal peptide probability: 1.000Max cleavage site probability: 0.768 between pos. 24 and 25BAASS:P77853 3^(rd) connection variant

(SEQ ID NO: 33) MANKHLSLSLFLVLLGLSASLASGQTSITLTSNASGTFDGYYYELWKDTGNTTMTVYTQGRFSCQWSNINNALFRTGKKYNQNWQSLGTIRITYSATYNPNGNSYLCIYGWSTNPLVEFYIVESWGNWRPPGATSLGQVTIDGGTYDIYRTTRVNQPSIVGTATFDQYWSVRTSKRTSGTVTVTDHFRAWANRGLNLGTIDQITLCVEGYQSSGSANITQNTFSQGSSSGSSGGSSGSTTTTRIECENMSLSGPYVSRITNPFNGIALYANGDTARATVNFPASRNYNFRLRGCGNNNNLARVDLRIDGRTVGTFYYQGTYPWEAPIDNVYVSAGSHTVEITVTADNGTW DVYADYLVIQSignalP3.0 Server Prediction: Signal peptideMost likely cleavage site between pos. 24 and 25: ASG-QTSignal peptide probability: 1.000Max cleavage site probability: 0.582 between pos. 24 and 25

In this example, the 2^(nd) variant of connection between BAASS [SEQ IDNO:8] and P77853 ( . . . GQ QTS . . . ), was selected for pAG2014 vectordevelopment based on the maximum cleavage site probability outputresults from the ServerP 3.0.

The individual genetic elements for pAG2014 construction were assembledin primary PCR reactions as depicted below. The first PCR reaction(PCR-1) was used to amplify 372 bp of the 3′ end of the rice Ubiquitin 3gene first intron (shown in low case letters) starting from its ownBglII site (underlined). The fragment was linked to the 9nt sequence(presented as Italics capital letters) representing modified threeinitial codons of the rice Ubiquitin 3 gene (detailed description isprovided above), BAASS (shown in capital letters) and 27 nt sequence(boxed) of the 5′ end of the coding region of P77853 mature protein. Thesecond PCR reaction (PCR-2) was performed to amplify the entire codingregion of P77853 mature protein fused to the TAG stop codon followed bythe AvrII restriction site (underlined).

-   -   1. PCR-1 to amplify 372 bp 3′ end of the rice Ubiquitin 3 gene        first intron, 9 bp junction sequence, BAASS and 5′ end of        P77853:

PCR-1 Product

(SEQ ID NO: 34)AgatctgttgtcctgtagttacttatgtcagttttgttattatctgaagatatttttggttgttgcttgttgatgtggtgtgagctgtgagcagcgctcttatgattaatgatgctgtccaattgtagtgtagtatgatgtgattgatatgttcatctattttgagctgacagtaccgatatcgtaggatctggtgccaacttattctccagctgcttttttttacctatgttaattccaatcctttcttgcctcttccagATCCAGATA ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTT

Primers

ovb79: (SEQ ID NO: 35) agatctgttgtcctgtagttacttatgtc ovb86: (SEQ ID NO:36) CCGACAGGCCAAGGAGGACGAGGAAGAGGGAGAGGGACAAATGTTTGTTCGCCATTATCTGGATctggaagaggcaagaaaggattggaa

ovb101:

(SEQ ID NO: 37) GTTGGATGTCAGAGTAATGCTTGTTTGTTGCCCGGAGGCCAAGCTGGCCGACAGGCCAAGGAGGAC

-   -   2. PCR-2 to amplify 1017 bp coding region of mature P77853        protein:

PCR-2 Product

(SEQ ID NO: 38) CAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTGGGCCAAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGACACGCGTCAACCAGCCTTCCATTGTGGGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCTGGGCGAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTACATTGTGCGTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAGAACACCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCATCCGGCTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTCCTTGTCCGGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATTGCGCTGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCCCCGCAAGTCGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAACAATAATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTCGGGACCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAATTGACAATGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGTTACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGG TGATACAGTGACCTAGG

Primers

ovb93: (SEQ ID NO: 39) CAAACAAGCATTACTCTGACATCCAAC ovb95: (SEQ ID NO:40) CCTAGGTCACTGTATCACCAGGTAGTCGGCAT

The subsequent “fusion PCR” approach (Yon and Fried, 1989) was utilizedto “stitch” together genetic elements prepared in PCR-1 and PCR-2. Thisapproach generated the expected 1362 bp BglII-AvrII sequence consistingof 261 bp of the 3′ end of rice Ubiquitin 3 gene first intron with itsnative 3′ end BglII site, 9 nt connecting sequence between the intronand the ATG codon of the 75 bp BAASS signal sequence, and 1011 bp matureP77853 xylanase coding region terminating in TGA stop codon that isflanked by the AvrII restriction site:

3′OsUbi3Pint:BAASS:P77853 as BglII-AvrII

(SEQ ID NO: 41)agatctgttgtcctgtagttacttatgtcagttttgttattatctgaagatatttttggttgttgcttgttgatgtggtgtgagctgtgagcagcgctcttatgattaatgatgctgtccaattgtagtgtagtatgatgtgattgatatgttcatctattttgagctgacagtaccgatatcgtaggatctggtgccaacttattctccagctgcttttttttacctatgttaattccaatcctttcttgcctcttccagATCCAGATA ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTT

TACAACAATGACGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTGGGCCAAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGACACGCGTCAACCAGCCTTCCATTGTGGGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCTGGGCGAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTACATTGTGCGTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAGAACACCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCATCCGGCTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTCCTTGTCCGGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATTGCGCTGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCCCCGCAAGTCGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAACAATAATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTCGGGACCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAATTGACAATGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGTTACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACAGTGA CCTAGG

The fusion PCR product was subsequently excised from the gel, gelpurified using QIAquick Gel Extraction Kit (Cat. #28706) and ligated tothe pPCR-Blunt II TOPO vector. The fusion PCR product was completelysequenced using vector specific and gene specific primers. The sequenceverified fusion PCR fragment was released from the pPCR-Blunt II TOPOvector with BglII-AvrII digestion and cloned into pBluescript that wasprepared in the following manipulations:

-   -   1. Referring to FIG. 5, the 2362 bp KpnI-EcoRI fragment of        pAG2005, which includes OsUbi3 promoter fused to the sequence        CCCGGGTATTCAT        (SEQ ID NO: 42) with XmaI (underlined) and AvrII (boxed) sites        and a Nos terminator, was initially cloned into pBluescript to        give the pBSK:OsUbi3P:XmaI:AvrII:NosT vector.    -   2. Referring to FIG. 6, cloning of the L1 linker        GAATTCTTACATTAGCACTAGAGCTC (SEQ ID NO: 43) into EcoRI-SacI sites        of pBSK:OsUbi3P:XmaI:AvrII:NosT removed an extra XmaI site and        produced the “shuttle” vector pBSK:OsUbi3P:XmaI:AvrII:NosT:L1:

pBSK:OsUbi3P:XmaI:AvrII:NosT:L1 readily accepts BglII-AvrII digested DNAfragments. In this manner, cloning fusion PCR products similar to thatdescribed in the above example, would lead to reconstruction of theentire expression cassette for the gene of interest. For example, the1362 bp BglII-AvrII digested fusion PCR product described above forP77853 was inserted in the BglII-AvrII digestedpBSK:OsUbi3P:XmaI:AvrII:NosT:L1 to create the OsUbi3P:BAASS:P77853:NosTexpression cassette.

The entire expression cassette OsUbi3P:BAASS:P77853:NosT was furtherexcised as a KpnI-EcoRI fragment using restriction enzymes and clonedinto pAG2005 to generate the pAG2014. The pAG2014 vector can be used forexpressing the wild type P77853 xylanase in transgenic plants from therice Ubiquitin 3 gene promoter, and targeting expressed enzyme to theplant cell wall by the barley alpha amylase signal sequence (BAASS).Using the same process, vectors in the following list were generated.The list below also includes pAG1000, 1002, 1003, 1004, 1005, 2000,2004. The vectors below may be utilized for plant transformation andexpression of the transgenes.

-   -   1. pAG1000-pAG1002 (SEQ ID NOS: 188-190, respectively) are        CMPSP:PMI in pSB11 with various restriction sites removed.    -   2. pAG1003 (SEQ ID NO: 191) is pAG1002 with an MCS.    -   3. pAG1004 is pAG1003 with GUS-int in the MCS.    -   4. pAG1005 (SEQ ID NO: 192) is pAG1003 with CPMSP:PMI, where PMI        is codon and expression optimized for maize.    -   5. pAG2000 (SEQ ID NO: 193) is pAG1003 with one connection of        the rice Ubi3 promoter and PMI in HindIII-SpeI replacing        CMPSP:PMI.    -   6. pAG2001 (SEQ ID NO: 194) is pAG2000 with the rice Ubi3        promoter in the MCS.    -   7. pAG2002 (SEQ ID NO: 195) is pAG2001 with the rice Ubi3        promoter and the Nos terminator in the MCS.    -   8. pAG2003 (SEQ ID NO: 196) is pAG2000 with second connection        between the rice Ubi3 promoter and PMI.    -   9. pAG2004 (SEQ ID NO: 197) is pAG2000 with third connection        between rice Ubi3 promoter and PMI.    -   10. pAG2005 (SEQ ID NO: 198) is pAG2004 with the added rice Ubi3        promoter and Nos terminator from pAG2002 in the MCS.    -   11. pAG2006 (SEQ ID NO: 199) is pAG2005 with GUS between the        rice Ubi3 promoter and the Nos terminator, using one connection        between the OsUbi3P and GUS.    -   12. pAG2007 (SEQ ID NO: 200) is pAG2005 with GUS between the        rice Ubi3 promoter and the Nos terminator, using a second        connection between OsUbi3P and GUS.    -   13. pAG2009 (SEQ ID NO: 201) is pAG2005 with GUS fused to the        PR1a intracellular space localization signal sequence (using one        connection) and between the rice Ubi3 promoter and the Nos        terminator.    -   14. pAG2010 (SEQ ID NO: 202) is pAG2005 with GUS fused to the        PR1a intracellular space localization signal sequence (using        second connection) and between the rice Ubi3 promoter and the        Nos terminator.    -   15. pAG2011 (SEQ ID NO: 203) is pAG2005 with GUS fused to the        BAASS cell wall targeting signal sequence and between the rice        Ubi3 promoter and the Nos terminator.    -   16. pAG2012 (SEQ ID NO: 204) is pAG2007 with GUS between the        rice glutelin GluB-4 promoter and the Nos terminator.    -   17. pAG2013 (SEQ ID NO: 205) is pAG2005 with GUS fused to the        HvExoI cell wall targeting signal sequence and between the rice        the Ubi3 promoter and the Nos terminator.    -   18. pAG2014 (SEQ ID NO: 206) is pAG2005 with WT P77853 fused to        the BAASS cell wall targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator.    -   19. pAG2015 (SEQ ID NO: 207) is pAG2005 with WT P77853 between        rice Ubi3 promoter and Nos terminator.    -   20. pAG2016 (SEQ ID NO: 208) is pAG2005 with GUS fused to the        PR1a (maize expression optimized) intracellular space        localization signal and between the rice Ubi3 promoter sequence        and the Nos terminator.    -   21. pAG2017 (SEQ ID NO: 209) is pAG2005 with WT P40942 fused to        the PR1a (maize expression optimized) intracellular space        localization signal and between the rice Ubi3 promoter sequence        and the Nos terminator.    -   22. pAG2018 (SEQ ID NO: 210) is pAG2005 with WT O30700 fused to        the BAASS cell wall targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator    -   23. pAG2019 (SEQ ID NO: 211) is pAG2005 with WT P40942 fused to        the BAASS cell wall targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator    -   24. pAG2020 (SEQ ID NO: 212) is pAG2005 with WT P77853 fused to        the PR1a (maize expression optimized) intracellular space        localization signal and between the rice Ubi3 promoter sequence        and the Nos terminator.    -   25. pAG2021 (SEQ ID NO: 213) is pAG2005 with P77853m3 fused to        the PR1a (maize expression optimized) intracellular space        localization signal and between the rice Ubi3 promoter sequence        and the Nos terminator.    -   26. pAG2022 (SEQ ID NO: 214) is pAG2005 with P77853m3:SEKDEL        fused to the PR1a (maize expression optimized) intracellular        space localization signal sequence and between the rice Ubi3        promoter and the Nos terminator.    -   27. pAG2023 (SEQ ID NO: 215) is pAG2005 with P77853m3 fused to        the BAASS cell wall targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator.    -   28. pAG2024 (SEQ ID NO: 216) is pAG2005 with P77853m3:SEKDEL        fused to the BAASS cell wall targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   29. pAG2025 (SEQ ID NO: 217) is pAG2012 with WT P77853 fused to        GluB-4 signal sequence and between the rice glutelin GluB-4        promoter and the Nos terminator.    -   30. pAG2026 (SEQ ID NO: 218) is pAG2012 with WT O30700 fused to        GluB-4 signal sequence and between the rice glutelin GluB-4        promoter and the Nos terminator.    -   31. pAG2027 (SEQ ID NO: 219) is pAG2012 with WT P40942 fused to        GluB-4 signal sequence and between the rice glutelin GluB-4        promoter and the Nos terminator.    -   32. pAG2028 (SEQ ID NO: 220) is pAG2005 with P77853T134-195        fused to the PR1a (maize expression optimized) intracellular        space localization signal sequence and between the rice Ubi3        promoter and the Nos terminator.    -   33. pAG2029 (SEQ ID NO: 221) is pAG2005 with P77853T134-195        fused to the BAASS cell wall targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   34. pAG2030 (SEQ ID NO: 222) is pAG2005 with P77853m3 between        the rice Ubi3 promoter and the Nos terminator.    -   35. pAG2031 (SEQ ID NO: 223) is pAG2012 with WT P54583 fused to        GluB-4 signal sequence and between the rice glutelin GluB-4        promoter and the Nos terminator.    -   36. pAG2032 (SEQ ID NO: 224) is pAG2012 with WT P54583:SEKDEL        fused to GluB-4 signal sequence and between the rice glutelin        GluB-4 promoter and the Nos terminator.    -   37. pAG2033 (SEQ ID NO: 225) is pAG2005 with WT P54583 between        the rice Ubi3 promoter and the Nos terminator.    -   38. pAG2034 (SEQ ID NO: 226) is pAG2005 with WT P54583:SEKDEL        between the rice Ubi3 promoter and the Nos terminator.    -   39. pAG2035 (SEQ ID NO: 227) is pAG2005 with WT P54583 fused to        PR1a (maize expression optimized) intracellular space        localization signal and between the rice Ubi3 promoter sequence        and the Nos terminator.    -   40. pAG2036 (SEQ ID NO: 228) is pAG2005 with WT P54583:SEKDEL        fused to the PR1a (maize expression optimized) intracellular        space localization signal sequence between the rice Ubi3        promoter and the Nos terminator.    -   41. pAG2037 (SEQ ID NO: 229) is pAG2005 with WT P54583 fused to        the BAASS cell wall targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator.    -   42. pAG2038 (SEQ ID NO: 230) is pAG2005 with WT P54583:SEKDEL        fused to the BAASS cell wall targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   43. pAG2039 (SEQ ID NO: 231) is pAG2005 with GUS fused to        HvAleSP and between the rice Ubi3 promoter and the Nos        terminator.    -   44. pAG2040 (SEQ ID NO: 232) is pAG2005 with WT NtEGm fused to        the BAASS cell wall targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator.    -   45. pAG2042 (SEQ ID NO: 234) is pAG2005 with WT P54583 fused to        the HvAleSP vacuole targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator.    -   46. pAG2043 (SEQ ID NO: 235) is pAG2005 with WT NtEGm between        the rice Ubi3 promoter and the Nos terminator.    -   47. pAG2044 (SEQ ID NO: 236) is pAG2005 with WT NtEGm fused to        the PR1a (maize expression optimized) intracellular space        localization signal sequence and between the rice Ubi3 promoter        and the Nos terminator.    -   48. pAG2045 (SEQ ID NO: 237) is pAG2005 with WT NtEGm:SEKDEL        fused to the PR1a (maize expression optimized) intracellular        space localization signal sequence and between the rice Ubi3        promoter and the Nos terminator.    -   49. pAG2046 (SEQ ID NO: 238) is pAG2005 with WT NtEGm:SEKDEL        fused to the BAASS cell wall targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   50. pAG2047 (SEQ ID NO: 239) is pAG2005 with WT P54583:SEKDEL        fused to the HvAleSP vacuole targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   51. pAG2048 (SEQ ID NO: 240) is pAG2005 with WT NtEGm between        the rice Ubi3 promoter fused to HvAleSP vacuole targeting signal        sequence and the Nos terminator.    -   52. pAG2049 (SEQ ID NO: 241) is pAG2005 with WT NtEGm:SEKDEL        fused to the HvAleSP vacuole targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   53. pAG2050 (SEQ ID NO: 242) is pAG2005 with WT P26222 between        the rice Ubi3 promoter and the Nos terminator.    -   54. pAG2051 (SEQ ID NO: 243) is pAG2005 with WT P26222 fused to        the PR1a (maize expression optimized) intracellular space        localization signal sequence and between the rice Ubi3 promoter        and the Nos terminator.    -   55. pAG2052 (SEQ ID NO: 244) is pAG2005 with WT P26222:SEKDEL        fused to the PR1a (maize expression optimized) intracellular        space localization signal sequence and between rice Ubi3        promoter and Nos terminator.    -   56. pAG2053 (SEQ ID NO: 245) is pAG2005 with WT P26222 fused to        the BAASS cell wall targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator.    -   57. pAG2054 (SEQ ID NO: 246) is pAG2005 with WT P26222:SEKDEL        fused to the BAASS cell wall targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   58. pAG2055 (SEQ ID NO: 247) is pAG2005 with WT P26222 fused to        the HvAleSP vacuole targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator.    -   59. pAG2056 (SEQ ID NO: 248) is pAG2005 with WT P26222:SEKDEL        fused to the HvAleSP vacuole targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   60. pAG2057 (SEQ ID NO: 249) is pAG2005 with WT P77853:SEKDEL        fused to the BAASS cell wall targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   61. pAG2058 (SEQ ID NO: 250) is pAG2005 with WT P77853:SEKDEL        fused to the PR1a (maize expression optimized) intracellular        space localization signal sequence and between the rice Ubi3        promoter and the Nos terminator.    -   62. pAG2059 (SEQ ID NO: 251) is pAG2005 with WT 043097 between        the rice Ubi3 promoter and the Nos terminator.    -   63. pAG2060 (SEQ ID NO: 252) is pAG2005 with WT 043097 fused to        the PR1a (maize expression optimized) intracellular space        localization signal sequence and between the rice Ubi3 promoter        and the Nos terminator.    -   64. pAG2061 (SEQ ID NO: 253) is pAG2005 with WT 043097:SEKDEL        fused to the PR1a (maize expression optimized) intracellular        space localization signal sequence and between the rice Ubi3        promoter and the Nos terminator.    -   65. pAG2062 (SEQ ID NO: 254) is pAG2005 with WT 043097 fused to        the BAASS cell wall targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator.    -   66. pAG2063 (SEQ ID NO: 255) is pAG2005 with WT 043097:SEKDEL        fused to the BAASS cell wall targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   67. pAG2064 (SEQ ID NO: 256) is pAG2005 with WT 043097 fused to        the HvAleSP vacuole targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator.    -   68. pAG2065 (SEQ ID NO: 257) is pAG2005 with WT 043097:SEKDEL        fused to the HvAleSP vacuole targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   69. pAG2066 (SEQ ID NO: 258) is pAG2005 with P77853-S158-2        intein modified xylanase fused to the BAASS cell wall targeting        signal sequence and between the rice Ubi3 promoter and the Nos        terminator.    -   70. pAG2067 (SEQ ID NO: 259) is pAG2005 with P77853-S158-19        intein modified xylanase fused to the BAASS cell wall targeting        signal sequence and between the rice Ubi3 promoter and the Nos        terminator.    -   71. pAG2068 (SEQ ID NO: 260) is pAG2005 with P77853-T134-1        intein modified xylanase fused to the BAASS cell wall targeting        signal sequence and between the rice Ubi3 promoter and the Nos        terminator.    -   72. pAG2069 (SEQ ID NO: 261) is pAG2005 with WT 068438 between        the rice Ubi3 promoter and the Nos terminator.    -   73. pAG2070 (SEQ ID NO: 262) is pAG2005 with WT 068438 fused to        the PR1a (maize expression optimized) intracellular space        localization signal and between the rice Ubi3 promoter sequence        and the Nos terminator.    -   74. pAG2071 (SEQ ID NO: 263) is pAG2005 with WT 068438:SEKDEL        fused to the PR1a (maize expression optimized) intracellular        space localization signal sequence and between the rice Ubi3        promoter and the Nos terminator.    -   75. pAG2072 (SEQ ID NO: 264) is pAG2005 with WT 068438 fused to        the BAASS cell wall targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator.    -   76. pAG2073 (SEQ ID NO: 265) is pAG2005 with WT 068438:SEKDEL        fused to the BAASS cell wall targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   77. pAG2074 (SEQ ID NO: 266) is pAG2005 with WT 068438 fused to        the HvAleSP vacuole targeting signal sequence and between the        rice Ubi3 promoter and the Nos terminator.    -   78. pAG2075 (SEQ ID NO: 267) is pAG2005 with WT 068438:SEKDEL        fused to the HvAleSP vacuole targeting signal sequence and        between the rice Ubi3 promoter and the Nos terminator.    -   79. pAG2076 (SEQ ID NO: 268) is pAG2005 with P77853-S158-2        intein modified xylanase between the rice Ubi3 promoter and the        Nos terminator.    -   80. pAG2077 (SEQ ID NO: 269) is pAG2005 with P77853-S158-19        intein modified xylanase between the rice Ubi3 promoter and the        Nos terminator.    -   81. pAG2078 (SEQ ID NO: 270) is pAG2005 with P77853-T134-1        intein modified xylanase between the rice Ubi3 promoter and the        Nos terminator.    -   82. pAG2079 (SEQ ID NO: 271) is pAG2005 with        P77853-5158-2:SEKDEL intein modified xylanase fused to BAASS        cell wall targeting signal sequence and between the rice Ubi3        promoter and the Nos terminator.    -   83. pAG2080 (SEQ ID NO: 272) is pAG2005 with        P77853-5158-19:SEKDEL intein modified xylanase fused to the        BAASS cell wall targeting signal sequence and between the rice        Ubi3 promoter and the Nos terminator.    -   84. pAG2081 (SEQ ID NO: 273) is pAG2005 with        P77853-T134-1:SEKDEL intein modified xylanase fused to the BAASS        cell wall targeting signal sequence and between the rice Ubi3        promoter and the Nos terminator.    -   85. pAG3000 (SEQ ID NO: 280) is pAG1003 with rice Act1 promoter        driving PMI in place of CMPSP:PMI using one connection between        OsAct1P and PMI (partial eukaryotic translation initiation site        consensus sequence).    -   86. pAG3001 (SEQ ID NO: 281) is pAG1003 with rice Act1 promoter        driving PMI in place of CMPS-PMI using second connection between        OsAct1P and PMI (complete eukaryotic translation initiation site        consensus sequence).    -   87. pAG3002 (SEQ ID NO: 282) is pAG3000 with GUS between the        rice Ubi3 promoter fused to BAASS cell wall targeting signal        sequence and the Nos terminator.    -   88. pAG3003 (SEQ ID NO: 283) is pAG3001 with GUS fused to the        BAASS cell wall targeting signal sequence and between the rice        Ubi3 promoter and the Nos terminator.    -   89. pAG2041 (SEQ ID NO: 233) is pAG2004 with NosT cloned into        the AvrII-EcoRI sites.    -   90. pAG2082 (SEQ ID NO: 274) is pAG2005 with WT 043097 fused to        Glutelin B-4 signal peptide and between rice the Glutelin B-4        promoter and the Nos terminator.    -   91. pAG2083 (SEQ ID NO: 275) is pAG2005 with WT 043097:SEKDEL        fused to Glutelin B-4 signal peptide and between the rice        Glutelin B-4 promoter and the Nos terminator.    -   92. pAG2084 (SEQ ID NO: 276) is pAG2005 with WT NtEGm fused to        Glutelin B-4 signal peptide and between the rice Glutelin B-4        promoter and the Nos terminator.    -   93. pAG2085 (SEQ ID NO: 275) is pAG2005 with P77853-T145-307        intein modified xylanase between the rice Ubi3 promoter and the        Nos terminator.    -   94. pAG2086 (SEQ ID NO: 278) is pAG2005 with P77853-T145-307        intein modified xylanase fused to BAASS cell wall targeting        signal sequence and between the rice Ubi3 promoter and Nos        terminator.    -   95. pAG2087 (SEQ ID NO: 279) is pAG2005 with        P77853-T145-307:SEKDEL intein modified xylanase fused to BAASS        cell wall targeting signal sequence and between the rice Ubi3        promoter and the Nos terminator.        The amino acid sequence of the protein encoded in each of the        above listed vectors 18-19, 21-84 and 89-95 and the nucleic acid        encoding the protein is provided in Table 1, below.

Embodiments herein include but are not limited to the gene sequencesunder the heading “Nucleotide sequence” in Table 1, below, the aminoacid sequences under the heading “Protein sequence” in Table 1, plantsincluding the gene sequences in Table 1, vectors including the genesequences in Table 1, the vectors under the heading “pAG vector” inTable 1, plants including the vectors in Table 1, plants includingproteins encoded by the Nucleotide sequences in Table 1 and plantsincluding the Protein sequences in Table 1. For the vectors in Table 1,each entry under the “pAG vector” heading includes a number. The numberis added to “pAG” to complete the vector name. For example, the listing“2014” is for the vector pAG2014.

TABLE 1 Sequences of CWDEs and their fusions in vectors pAG Sequencevector annotation Protein sequence Nucleotide sequence 2014 BAASS:P77853MANKHLSLSLFLVLLG ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTGLSASLASGQQTSITLT GCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCTSNASGTFDGYYYELWK GACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTCDTGNTTMTVYTQGRFS TGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTCCQWSNINNALFRTGKK GCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGACYNQNWQSLGTIRITYS CGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGGATYNPNGNSYLCIYGW ATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGTSTNPLVEFYIVESWGN GTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGTWRPPGATSLGQVTIDG TGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTGGGCGTYDIYRTTRVNQPSI CAAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGACACVGTATFDQYWSVRTSK GCGTCAACCAGCCTTCCATTGTGGGGACAGCCACGTTCGATCAGTARTSGTVTVTDHFRAWA CTGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAACAGTGACCGTGNRGLNLGTIDQITLCV ACCGATCACTTCCGCGCCTGGGCGAACCGGGGCCTGAACCTCGGCAEGYQSSGSANITQNTF CAATAGACCAAATTACATTGTGCGTGGAGGGTTACCAAAGCTCTGGSQGSSSGSSGGSSGST ATCAGCCAACATCACCCAGAACACCTTCTCTCAGGGCTCTTCTTCCTTTRIECENMSLSGPY GGCAGTTCGGGTGGCTCATCCGGCTCCACAACGACTACTCGCATCGVSRITNPFNGIALYAN AGTGTGAGAACATGTCCTTGTCCGGACCCTACGTTAGCAGGATCACGDTARATVNFPASRNY CAATCCCTTTAATGGTATTGCGCTGTACGCCAACGGAGACACAGCCNFRLRGCGNNNNLARV CGCGCTACCGTTAACTTCCCCGCAAGTCGCAACTACAATTTCCGCCDLRIDGRTVGTFYYQG TGCGGGGTTGCGGCAACAACAATAATCTTGCCCGTGTGGACCTGAGTYPWEAPIDNVYVSAG GATCGACGGACGGACCGTCGGGACCTTTTATTACCAGGGCACATACSHTVEITVTADNGTWD CCCTGGGAGGCCCCAATTGACAATGTTTATGTCAGTGCGGGGAGTCVYADYLVIQ* (SEQ ATACAGTCGAAATCACTGTTACTGCGGATAACGGCACATGGGACGT ID NO:44) GTATGCCGACTACCTGGTGATACAGTGA (SEQ ID NO: 116) 2015 P77853MQTSITLTSNASGTFD ATGCAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTGGYYYELWKDTGNTTMT ACGGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAATVYTQGRFSCQWSNINN GACGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCGAACATCALFRTGKKYNQNWQSL AATAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTGGCGTIRITYSATYNPNGN AGTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAACCCSYLCIYGWSTNPLVEF AAACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACCCAYIVESWGNWRPPGATS TTGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACCGCLGQVTIDGGTYDIYRT CTGGTGCCACGTCCCTGGGCCAAGTGACAATCGATGGCGGGACCTATRVNQPSIVGTATFDQ CGACATCTATAGGACGACACGCGTCAACCAGCCTTCCATTGTGGGGYWSVRTSKRTSGTVTV ACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCTAAGCGGATDHFRAWANRGLNLGT CTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCTGGGCGAAIDQITLCVEGYQSSGS CCGGGGCCTGAACCTCGGCACAATAGACCAAATTACATTGTGCGTGANITQNTFSQGSSSGS GAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAGAACACCTSGGSSGSTTTTRIECE TCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCATCCGGCTCNMSLSGPYVSRITNPF CACAACGACTACTCGCATCGAGTGTGAGAACATGTCCTTGTCCGGANGIALYANGDTARATV CCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATTGCGCTGTNFPASRNYNFRLRGCG ACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCCCCGCAAGNNNNLARVDLRIDGRT TCGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAACAATAATVGTFYYQGTYPWEAPI CTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTCGGGACCTDNVYVSAGSHTVEITV TTTATTACCAGGGCACATACCCCTGGGAGGCCCCAATTGACAATGTTADNGTWDVYADYLVI TTATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGTTACTGCG Q* (SEQID NO: GATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACAGT 45) GA (SEQ ID NO:117) 2017 PR1a:O40942 MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT TLLLFLVISHSCRAFNCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCTT DQTSAEDIPSLAEAFRCAACGACCAGACAAGTGCAGAGGATATTCCGTCACTTGCCGAAGCG DYFPIGAAIEPGYTTGTTCAGGGACTATTTCCCTATCGGAGCTGCCATTGAGCCGGGCTATA QIAELYKKHVNMLVAECCACGGGTCAGATTGCCGAATTGTACAAGAAACACGTGAATATGCT NAMKPASLQPTEGNFQGGTCGCGGAGAACGCTATGAAGCCCGCCTCGCTCCAGCCGACGGAG WADADRIVQFAKENGMGGTAATTTTCAGTGGGCCGACGCGGACCGCATTGTTCAGTTCGCTA ELRFHTLVWHNQTPTGAGGAAAACGGAATGGAGCTTCGGTTTCACACGTTGGTGTGGCACAA FSLDKEGKPMVEETDPTCAAACCCCAACTGGCTTCAGCCTGGATAAGGAAGGGAAACCTATG QKREENRKLLLQRLENGTCGAGGAAACGGACCCTCAAAAGAGAGAAGAGAACAGGAAACTCC YIRAVVLRYKDDIKSWTTTTGCAGCGCCTCGAAAACTATATCCGGGCCGTTGTGTTGAGATA DVVNEVIEPNDPGGMRCAAGGATGACATCAAGTCCTGGGATGTTGTCAATGAGGTTATAGAA NSPWYQITGTEYIEVACCAAACGACCCAGGGGGTATGCGTAATTCTCCCTGGTATCAAATCA FRATREAGGSDIKLYICAGGAACCGAATATATTGAGGTCGCATTTCGCGCGACACGTGAAGC NDYNTDDPVKRDILYETGGCGGGTCAGATATAAAGCTGTATATTAATGATTACAATACGGAC LVKNLLEKGVPIDGVGGATCCTGTTAAACGGGATATACTCTACGAGCTTGTGAAGAACTTGC HQTHIDIYNPPVERIITGGAGAAAGGTGTCCCGATTGATGGCGTGGGACATCAGACACATAT ESIKKFAGLGLDNIITCGACATCTACAACCCACCCGTTGAAAGGATTATCGAGTCGATTAAG ELDMSIYSWNDRSDYGAAGTTCGCCGGACTCGGGCTTGATAATATCATTACCGAACTGGACA DSIPDYILTLQAKRYQTGAGCATCTATTCCTGGAATGATCGCTCTGACTACGGTGATTCAAT ELFDALKENKDIVSAVCCCTGACTATATTCTCACCTTGCAGGCCAAAAGATACCAGGAGCTT VFWGISDKYSWLNGFPTTCGATGCGCTGAAGGAGAATAAGGATATAGTCTCGGCTGTGGTCT VKRTNAPLLFDRNFMPTTTGGGGAATTAGCGACAAATACTCCTGGCTGAATGGCTTCCCGGT KPAFWAIVDPSRLRE*CAAGAGGACTAATGCCCCATTGCTGTTTGATCGCAACTTTATGCCT (SEQ ID NO: 46)AAACCAGCATTTTGGGCAATCGTGGACCCGAGTAGACTCAGGGAAT AA (SEQ ID NO: 118) 2018BAASS:O30700 MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQVQPFAWQGCCTGTCGGCCAGCTTGGCCTCCGGGCAAGTTCAGCCGTTCGCATG VASLADRYEESFDIGAGCAGGTGGCATCCCTGGCTGACCGCTACGAAGAGAGCTTCGATATT AVEPHQLNGRQGKVLKGGAGCGGCGGTGGAACCTCACCAATTGAACGGTCGCCAGGGGAAGG HHYNSIVAENAMKPISTCCTGAAGCATCACTATAACTCAATCGTGGCCGAGAATGCTATGAA LQPEEGVFTWDGADAIGCCGATCTCCCTCCAACCGGAAGAAGGAGTTTTCACGTGGGATGGA VEFARKNNMNLRFHTLGCTGATGCAATAGTGGAGTTCGCGCGGAAAAATAACATGAACCTGC VWHNQVPDWFFLDEEGGCTTTCACACGCTCGTGTGGCATAACCAAGTGCCCGACTGGTTCTT NPMVEETNEAKRQANKCCTGGACGAAGAGGGTAACCCTATGGTCGAGGAGACTAACGAAGCG ELLLERLETHIKTVVEAAAAGGCAAGCGAATAAAGAGCTTTTGCTTGAGAGACTTGAGACTC RYKDDVTAWDVVNEVVATATCAAAACTGTGGTCGAAAGGTACAAGGATGACGTTACGGCCTG DDGTPNERGLRESVWYGGATGTGGTGAATGAGGTTGTGGACGATGGCACCCCAAATGAAAGG QITGDEYIRVAFETARGGACTGCGCGAGAGCGTTTGGTATCAGATTACAGGCGATGAATACA KYAGEDAKLFINDYNTTTAGAGTGGCATTCGAGACTGCGCGCAAGTACGCTGGCGAAGACGC EVTPKRDHLYNLVQDLTAAGCTGTTCATCAACGACTACAACACGGAGGTGACACCCAAGCGC LADGVPIDGVGHQAHIGATCACCTCTACAACTTGGTTCAAGACCTGCTCGCGGACGGGGTCC QIDWPTIDEIRTSMEMCGATCGATGGAGTGGGACATCAAGCCCATATCCAGATCGATTGGCC FAGLGLDNQVTELDVSCACCATCGATGAGATCAGGACCTCGATGGAGATGTTTGCCGGCCTT LYGWPPRPAFPTYDAIGGGCTCGACAACCAAGTTACCGAACTCGATGTTTCCTTGTACGGTT PQERFQAQADRYNQLFGGCCGCCTCGCCCGGCATTCCCGACCTACGATGCAATCCCTCAAGA ELYEELDADLSSVTFWGAGGTTTCAGGCGCAGGCGGATAGATACAATCAGCTCTTCGAGCTT GIADNHTWLDDRAREYTACGAGGAACTCGACGCTGACCTCTCAAGCGTGACCTTCTGGGGGA NDGVGKDAPFVFDPNYTCGCGGACAACCATACCTGGCTCGACGACAGGGCCAGAGAATACAA RVKPAFWRIID*TGACGGGGTCGGCAAAGATGCCCCGTTCGTCTTCGATCCGAACTAC (SEQ ID NO: 47)AGGGTTAAACCTGCCTTCTGGCGCATCATTGACTGA (SEQ ID NO: 119) 2019 BAASS:P40942MANKHLSLSLFLVLLG ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTGLSASLASGQVFNDQTS GCCTGTCGGCCAGCTTGGCCTCCGGGCAAGTCTTCAACGACCAGACAEDIPSLAEAFRDYFP AAGTGCAGAGGATATTCCGTCACTTGCCGAAGCGTTCAGGGACTATIGAAIEPGYTTGQIAE TTCCCTATCGGAGCTGCCATTGAGCCGGGCTATACCACGGGTCAGALYKKHVNMLVAENAMK TTGCCGAATTGTACAAGAAACACGTGAATATGCTGGTCGCGGAGAAPASLQPTEGNFQWADA CGCTATGAAGCCCGCCTCGCTCCAGCCGACGGAGGGTAATTTTCAGDRIVQFAKENGMELRF TGGGCCGACGCGGACCGCATTGTTCAGTTCGCTAAGGAAAACGGAAHTLVWHNQTPTGFSLD TGGAGCTTCGGTTTCACACGTTGGTGTGGCACAATCAAACCCCAACKEGKPMVEETDPQKRE TGGCTTCAGCCTGGATAAGGAAGGGAAACCTATGGTCGAGGAAACGENRKLLLQRLENYIRA GACCCTCAAAAGAGAGAAGAGAACAGGAAACTCCTTTTGCAGCGCCVVLRYKDDIKSWDVVN TCGAAAACTATATCCGGGCCGTTGTGTTGAGATACAAGGATGACATEVIEPNDPGGMRNSPW CAAGTCCTGGGATGTTGTCAATGAGGTTATAGAACCAAACGACCCAYQITGTEYIEVAFRAT GGGGGTATGCGTAATTCTCCCTGGTATCAAATCACAGGAACCGAATREAGGSDIKLYINDYN ATATTGAGGTCGCATTTCGCGCGACACGTGAAGCTGGCGGGTCAGATDDPVKRDILYELVKN TATAAAGCTGTATATTAATGATTACAATACGGACGATCCTGTTAAALLEKGVPIDGVGHQTH CGGGATATACTCTACGAGCTTGTGAAGAACTTGCTGGAGAAAGGTGIDIYNPPVERIIESIK TCCCGATTGATGGCGTGGGACATCAGACACATATCGACATCTACAAKFAGLGLDNIITELDM CCCACCCGTTGAAAGGATTATCGAGTCGATTAAGAAGTTCGCCGGASIYSWNDRSDYGDSIP CTCGGGCTTGATAATATCATTACCGAACTGGACATGAGCATCTATTDYILTLQAKRYQELFD CCTGGAATGATCGCTCTGACTACGGTGATTCAATCCCTGACTATATALKENKDIVSAVVFWG TCTCACCTTGCAGGCCAAAAGATACCAGGAGCTTTTCGATGCGCTGISDKYSWLNGFPVKRT AAGGAGAATAAGGATATAGTCTCGGCTGTGGTCTTTTGGGGAATTANAPLLFDRNFMPKPAF GCGACAAATACTCCTGGCTGAATGGCTTCCCGGTCAAGAGGACTAAWAIVDPSRLRE* (SEQ TGCCCCATTGCTGTTTGATCGCAACTTTATGCCTAAACCAGCATTT ID NO:48) TGGGCAATCGTGGACCCGAGTAGACTCAGGGAATAA (SEQ ID NO: 120) 2020PR1a:P77853 MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT TLLLFLVISHSCRAQQCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCCA TSITLTSNASGTFDGYGCAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTGAC YYELWKDTGNTTMTVYGGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAATGA TQGRFSCQWSNINNALCGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCGAACATCAA FRTGKKYNQNWQSLGTTAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTGGCAG IRITYSATYNPNGNSYTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAACCCAA LCIYGWSTNPLVEFYIACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACCCATT VESWGNWRPPGATSLGGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACCGCCT QVTIDGGTYDIYRTTRGGTGCCACGTCCCTGGGCCAAGTGACAATCGATGGCGGGACCTACG VNQPSIVGTATFDQYWACATCTATAGGACGACACGCGTCAACCAGCCTTCCATTGTGGGGAC SVRTSKRTSGTVTVTDAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCTAAGCGGACT HFRAWANRGLNLGTIDTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCTGGGCGAACC QITLCVEGYQSSGSANGGGGCCTGAACCTCGGCACAATAGACCAAATTACATTGTGCGTGGA ITQNTFSQGSSSGSSGGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAGAACACCTTC GSSGSTTTTRIECENMTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCATCCGGCTCCA SLSGPYVSRITNPFNGCAACGACTACTCGCATCGAGTGTGAGAACATGTCCTTGTCCGGACC IALYANGDTARATVNFCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATTGCGCTGTAC PASRNYNFRLRGCGNNGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCCCCGCAAGTC NNLARVDLRIDGRTVGGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAACAATAATCT TFYYQGTYPWEAPIDNTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTCGGGACCTTT VYVSAGSHTVEITVTATATTACCAGGGCACATACCCCTGGGAGGCCCCAATTGACAATGTTT DNGTWDVYADYLVIQ*ATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGTTACTGCGGA (SEQ ID NO: 49)TAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACAGTGA (SEQ ID NO: 121) 2021PR1a:P77853m3 MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT TLLLFLVISHSCRAQQCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCCA TSITLTSNASGTFDGYGCAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTGAC YYELWKDTGNTTMTVYGGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAATGA TQGRFSCQWSNLPEEWCGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCTAATTTGCC VPLTKNGKSKTFRIGGAGAAGAGTGGGTTCCTTTAACTAAGAATGGTAAGTCAAAGACCTTT FVDGLMKANQGKVKKTAGAATTGGAGGCTTCGTAGACGGTTTGATGAAGGCTAACCAAGGAA GDTEVLEVAGIHANSFAGGTCAAGAAGACCGGTGACACCGAAGTATTAGAGGTTGCAGGTAT DRKSKKSRTMAVKAVICCATGCCAATTCCTTTGACAGAAAGTCAAAGAAGTCCAGAACCATG RHRYSGNVYRIVLNSGGCTGTAAAAGCAGTCATTAGACACAGATATTCCGGAAACGTGTACA RKITITEGHSLFVYRNGAATAGTTTTGAACTCCGGAAGAAAGATCACCATTACTGAGGGACA GDLVEATGEDVKIGDNTTCCTTATTCGTCTATAGAAACGGTGACTTGGTGGAAGCCACAGGT LAVPRSDGSGDITEDRGAGGATGTAAAGATAGGTGATAACTTAGCTGTTCCAAGAAGCGACG VVEIKREYYDGYVYDLGATCCGGAGACATTACTGAGGATAGAGTTGTAGAAATTAAGAGAGA SLDEDENFLAGHGYLMGTACTACGACGGTTATGTCTATGACTTGTCATTGGATGAAGATGAA AHNSNINNALFRTGKKAATTTCTTGGCAGGACACGGTTACTTGATGGCCCATAACTCGAACA YNQNWQSLGTIRITYSTCAATAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTG ATYNPNGNSYLCIYGWGCAGTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAAC STNPLVEFYIVESWGNCCAAACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACC WRPPGATSLGQVTIDGCATTGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACC GTYDIYRTTRVNQPSIGCCTGGTGCCACGTCCCTGGGCCAAGTGACAATCGATGGCGGGACC VGTATFDQYWSVRTSKTACGACATCTATAGGACGACACGCGTCAACCAGCCTTCCATTGTGG RTSGTVTVTDHFRAWAGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCTAAGCG NRGLNLGTIDQITLCVGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCTGGGCG EGYQSSGSANITQNTFAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTACATTGTGCG SQGSSSGSSGGSSGSTTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAGAACAC TTTRIECENMSLSGPYCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCATCCGGC VSRITNPFNGIALYANTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTCCTTGTCCG GDTARATVNFPASCNYGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATTGCGCT NFRLRGCGNNNNLARVGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCCCCGCA DLRIDGRTVGTFYYQGAGTTGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAACAATA TYPWEAPIDNVYVSAGATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTCGGGAC SHTVEITVTADNGTWDCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAATTGACAAT VYADYLVIQ* (SEQGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGTTACTG ID NO: 50)CGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACA GTGA (SEQ ID NO: 122)2022 PR1a:P77853m3: MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT SEKDEL TLLLFLVISHSCRAQQCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCCA TSITLTSNASGTFDGYGCAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTGAC YYELWKDTGNTTMTVYGGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAATGA TQGRFSCQWSNLPEEWCGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCTAATTTGCC VPLTKNGKSKTFRIGGAGAAGAGTGGGTTCCTTTAACTAAGAATGGTAAGTCAAAGACCTTT FVDGLMKANQGKVKKTAGAATTGGAGGCTTCGTAGACGGTTTGATGAAGGCTAACCAAGGAA GDTEVLEVAGIHANSFAGGTCAAGAAGACCGGTGACACCGAAGTATTAGAGGTTGCAGGTAT DRKSKKSRTMAVKAVICCATGCCAATTCCTTTGACAGAAAGTCAAAGAAGTCCAGAACCATG RHRYSGNVYRIVLNSGGCTGTAAAAGCAGTCATTAGACACAGATATTCCGGAAACGTGTACA RKITITEGHSLFVYRNGAATAGTTTTGAACTCCGGAAGAAAGATCACCATTACTGAGGGACA GDLVEATGEDVKIGDNTTCCTTATTCGTCTATAGAAACGGTGACTTGGTGGAAGCCACAGGT LAVPRSDGSGDITEDRGAGGATGTAAAGATAGGTGATAACTTAGCTGTTCCAAGAAGCGACG VVEIKREYYDGYVYDLGATCCGGAGACATTACTGAGGATAGAGTTGTAGAAATTAAGAGAGA SLDEDENFLAGHGYLMGTACTACGACGGTTATGTCTATGACTTGTCATTGGATGAAGATGAA AHNSNINNALFRTGKKAATTTCTTGGCAGGACACGGTTACTTGATGGCCCATAACTCGAACA YNQNWQSLGTIRITYSTCAATAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTG ATYNPNGNSYLCIYGWGCAGTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAAC STNPLVEFYIVESWGNCCAAACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACC WRPPGATSLGQVTIDGCATTGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACC GTYDIYRTTRVNQPSIGCCTGGTGCCACGTCCCTGGGCCAAGTGACAATCGATGGCGGGACC VGTATFDQYWSVRTSKTACGACATCTATAGGACGACACGCGTCAACCAGCCTTCCATTGTGG RTSGTVTVTDHFRAWAGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCTAAGCG NRGLNLGTIDQITLCVGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCTGGGCG EGYQSSGSANITQNTFAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTACATTGTGCG SQGSSSGSSGGSSGSTTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAGAACAC TTTRIECENMSLSGPYCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCATCCGGC VSRITNPFNGIALYANTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTCCTTGTCCG GDTARATVNFPASCNYGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATTGCGCT NFRLRGCGNNNNLARVGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCCCCGCA DLRIDGRTVGTFYYQGAGTTGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAACAATA TYPWEAPIDNVYVSAGATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTCGGGAC SHTVEITVTADNGTWDCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAATTGACAAT VYADYLVIQSEKDEL*GTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGTTACTG (SEQ ID NO: 51)CGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACA GAGCGAGAAGGACGAGCTGTGA(SEQ ID NO: 123) 2023 BAASS:P77853m3 MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQQTSITLTGCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCT SNASGTFDGYYYELWKGACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTC DTGNTTMTVYTQGRFSTGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTC CQWSNLPEEWVPLTKNGCTTTTCCTGCCAGTGGTCTAATTTGCCAGAAGAGTGGGTTCCTTT GKSKTFRIGGFVDGLMAACTAAGAATGGTAAGTCAAAGACCTTTAGAATTGGAGGCTTCGTA KANQGKVKKTGDTEVLGACGGTTTGATGAAGGCTAACCAAGGAAAGGTCAAGAAGACCGGTG EVAGIHANSFDRKSKKACACCGAAGTATTAGAGGTTGCAGGTATCCATGCCAATTCCTTTGA SRTMAVKAVIRHRYSGCAGAAAGTCAAAGAAGTCCAGAACCATGGCTGTAAAAGCAGTCATT NVYRIVLNSGRKITITAGACACAGATATTCCGGAAACGTGTACAGAATAGTTTTGAACTCCG EGHSLFVYRNGDLVEAGAAGAAAGATCACCATTACTGAGGGACATTCCTTATTCGTCTATAG TGEDVKIGDNLAVPRSAAACGGTGACTTGGTGGAAGCCACAGGTGAGGATGTAAAGATAGGT DGSGDITEDRVVEIKRGATAACTTAGCTGTTCCAAGAAGCGACGGATCCGGAGACATTACTG EYYDGYVYDLSLDEDEAGGATAGAGTTGTAGAAATTAAGAGAGAGTACTACGACGGTTATGT NFLAGHGYLMAHNSNICTATGACTTGTCATTGGATGAAGATGAAAATTTCTTGGCAGGACAC NNALFRTGKKYNQNWQGGTTACTTGATGGCCCATAACTCGAACATCAATAACGCGTTGTTTA SLGTIRITYSATYNPNGGACCGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAAT GNSYLCIYGWSTNPLVCCGGATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTAC EFYIVESWGNWRPPGATTGTGTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACA TSLGQVTIDGGTYDIYTCGTTGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCT RTTRVNQPSIVGTATFGGGCCAAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACG DQYWSVRTSKRTSGTVACACGCGTCAACCAGCCTTCCATTGTGGGGACAGCCACGTTCGATC TVTDHFRAWANRGLNLAGTACTGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAACAGTGAC GTIDQITLCVEGYQSSCGTGACCGATCACTTCCGCGCCTGGGCGAACCGGGGCCTGAACCTC GSANITQNTFSQGSSSGGCACAATAGACCAAATTACATTGTGCGTGGAGGGTTACCAAAGCT GSSGGSSGSTTTTRIECTGGATCAGCCAACATCACCCAGAACACCTTCTCTCAGGGCTCTTC CENMSLSGPYVSRITNTTCCGGCAGTTCGGGTGGCTCATCCGGCTCCACAACGACTACTCGC PFNGIALYANGDTARAATCGAGTGTGAGAACATGTCCTTGTCCGGACCCTACGTTAGCAGGA TVNFPASCNYNFRLRGTCACCAATCCCTTTAATGGTATTGCGCTGTACGCCAACGGAGACAC CGNNNNLARVDLRIDGAGCCCGCGCTACCGTTAACTTCCCCGCAAGTTGCAACTACAATTTC RTVGTFYYQGTYPWEACGCCTGCGGGGTTGCGGCAACAACAATAATCTTGCCCGTGTGGACC PIDNVYVSAGSHTVEITGAGGATCGACGGACGGACCGTCGGGACCTTTTATTACCAGGGCAC TVTADNGTWDVYADYLATACCCCTGGGAGGCCCCAATTGACAATGTTTATGTCAGTGCGGGG VIQ* (SEQ ID NO:AGTCATACAGTCGAAATCACTGTTACTGCGGATAACGGCACATGGG 52)ACGTGTATGCCGACTACCTGGTGATACAGTGA (SEQ ID NO: 124) 2024 BAASS:P77853m3:MANKHLSLSLFLVLLG ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG SEKDELLSASLASGQQTSITLT GCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCTSNASGTFDGYYYELWK GACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTCDTGNTTMTVYTQGRFS TGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTCCQWSNLPEEWVPLTKN GCTTTTCCTGCCAGTGGTCTAATTTGCCAGAAGAGTGGGTTCCTTTGKSKTFRIGGFVDGLM AACTAAGAATGGTAAGTCAAAGACCTTTAGAATTGGAGGCTTCGTAKANQGKVKKTGDTEVL GACGGTTTGATGAAGGCTAACCAAGGAAAGGTCAAGAAGACCGGTGEVAGIHANSFDRKSKK ACACCGAAGTATTAGAGGTTGCAGGTATCCATGCCAATTCCTTTGASRTMAVKAVIRHRYSG CAGAAAGTCAAAGAAGTCCAGAACCATGGCTGTAAAAGCAGTCATTNVYRIVLNSGRKITIT AGACACAGATATTCCGGAAACGTGTACAGAATAGTTTTGAACTCCGEGHSLFVYRNGDLVEA GAAGAAAGATCACCATTACTGAGGGACATTCCTTATTCGTCTATAGTGEDVKIGDNLAVPRS AAACGGTGACTTGGTGGAAGCCACAGGTGAGGATGTAAAGATAGGTDGSGDITEDRVVEIKR GATAACTTAGCTGTTCCAAGAAGCGACGGATCCGGAGACATTACTGEYYDGYVYDLSLDEDE AGGATAGAGTTGTAGAAATTAAGAGAGAGTACTACGACGGTTATGTNFLAGHGYLMAHNSNI CTATGACTTGTCATTGGATGAAGATGAAAATTTCTTGGCAGGACACNNALFRTGKKYNQNWQ GGTTACTTGATGGCCCATAACTCGAACATCAATAACGCGTTGTTTASLGTIRITYSATYNPN GGACCGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATGNSYLCIYGWSTNPLV CCGGATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACEFYIVESWGNWRPPGA TTGTGTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATSLGQVTIDGGTYDIY TCGTTGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTRTTRVNQPSIVGTATF GGGCCAAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGDQYWSVRTSKRTSGTV ACACGCGTCAACCAGCCTTCCATTGTGGGGACAGCCACGTTCGATCTVTDHFRAWANRGLNL AGTACTGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAACAGTGACGTIDQITLCVEGYQSS CGTGACCGATCACTTCCGCGCCTGGGCGAACCGGGGCCTGAACCTCGSANITQNTFSQGSSS GGCACAATAGACCAAATTACATTGTGCGTGGAGGGTTACCAAAGCTGSSGGSSGSTTTTRIE CTGGATCAGCCAACATCACCCAGAACACCTTCTCTCAGGGCTCTTCCENMSLSGPYVSRITN TTCCGGCAGTTCGGGTGGCTCATCCGGCTCCACAACGACTACTCGCPFNGIALYANGDTARA ATCGAGTGTGAGAACATGTCCTTGTCCGGACCCTACGTTAGCAGGATVNFPASCNYNFRLRG TCACCAATCCCTTTAATGGTATTGCGCTGTACGCCAACGGAGACACCGNNNNLARVDLRIDG AGCCCGCGCTACCGTTAACTTCCCCGCAAGTTGCAACTACAATTTCRTVGTFYYQGTYPWEA CGCCTGCGGGGTTGCGGCAACAACAATAATCTTGCCCGTGTGGACCPIDNVYVSAGSHTVEI TGAGGATCGACGGACGGACCGTCGGGACCTTTTATTACCAGGGCACTVTADNGTWDVYADYL ATACCCCTGGGAGGCCCCAATTGACAATGTTTATGTCAGTGCGGGGVIQSEKDEL* (SEQ AGTCATACAGTCGAAATCACTGTTACTGCGGATAACGGCACATGGG ID NO:53) ACGTGTATGCCGACTACCTGGTGATACAGAGCGAGAAGGACGAGCT GTGA (SEQ ID NO: 125)2025 GluB4SP:P77853 MATIAFSRLSIYFCVLATGGCCACCATCGCTTTCTCCCGCTTGTCCATCTACTTCTGCGTGC LLCHGSMAQTSITLTSTTCTCCTGTGCCACGGCTCCATGGCCCAAACAAGCATTACTCTGAC NASGTFDGYYYELWKDATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTCTGG TGNTTMTVYTQGRFSCAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTCGCT QWSNINNALFRTGKKYTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGACCGG NQNWQSLGTIRITYSAGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGGATC TYNPNGNSYLCIYGWSACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGTGTA TNPLVEFYIVESWGNWTCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGTTGA RPPGATSLGQVTIDGGGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTGGGCCAA TYDIYRTTRVNQPSIVGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGACACGCG GTATFDQYWSVRTSKRTCAACCAGCCTTCCATTGTGGGGACAGCCACGTTCGATCAGTACTG TSGTVTVTDHFRAWANGAGCGTGCGCACCTCTAAGCGGACTTCAGGAACAGTGACCGTGACC RGLNLGTIDQITLCVEGATCACTTCCGCGCCTGGGCGAACCGGGGCCTGAACCTCGGCACAA GYQSSGSANITQNTFSTAGACCAAATTACATTGTGCGTGGAGGGTTACCAAAGCTCTGGATC QGSSSGSSGGSSGSTTAGCCAACATCACCCAGAACACCTTCTCTCAGGGCTCTTCTTCCGGC TTRIECENMSLSGPYVAGTTCGGGTGGCTCATCCGGCTCCACAACGACTACTCGCATCGAGT SRITNPFNGIALYANGGTGAGAACATGTCCTTGTCCGGACCCTACGTTAGCAGGATCACCAA DTARATVNFPASRNYNTCCCTTTAATGGTATTGCGCTGTACGCCAACGGAGACACAGCCCGC FRLRGCGNNNNLARVDGCTACCGTTAACTTCCCCGCAAGTCGCAACTACAATTTCCGCCTGC LRIDGRTVGTFYYQGTGGGGTTGCGGCAACAACAATAATCTTGCCCGTGTGGACCTGAGGAT YPWEAPIDNVYVSAGSCGACGGACGGACCGTCGGGACCTTTTATTACCAGGGCACATACCCC HTVEITVTADNGTWDVTGGGAGGCCCCAATTGACAATGTTTATGTCAGTGCGGGGAGTCATA YADYLVIQ* (SEQCAGTCGAAATCACTGTTACTGCGGATAACGGCACATGGGACGTGTA ID NO: 54)TGCCGACTACCTGGTGATACAGTGA (SEQ ID NO: 126) 2026 GluB4SP:O30700MATIAFSRLSIYFCVL ATGGCCACCATCGCTTTCTCCCGCTTGTCCATCTACTTCTGCGTGCLLCHGSMAVQPFAWQV TTCTCCTGTGCCACGGCTCCATGGCCGTTCAGCCGTTCGCATGGCAASLADRYEESFDIGAA GGTGGCATCCCTGGCTGACCGCTACGAAGAGAGCTTCGATATTGGAVEPHQLNGRQGKVLKH GCGGCGGTGGAACCTCACCAATTGAACGGTCGCCAGGGGAAGGTCCHYNSIVAENAMKPISL TGAAGCATCACTATAACTCAATCGTGGCCGAGAATGCTATGAAGCCQPEEGVFTWDGADAIV GATCTCCCTCCAACCGGAAGAAGGAGTTTTCACGTGGGATGGAGCTEFARKNNMNLRFHTLV GATGCAATAGTGGAGTTCGCGCGGAAAAATAACATGAACCTGCGCTWHNQVPDWFFLDEEGN TTCACACGCTCGTGTGGCATAACCAAGTGCCCGACTGGTTCTTCCTPMVEETNEAKRQANKE GGACGAAGAGGGTAACCCTATGGTCGAGGAGACTAACGAAGCGAAALLLERLETHIKTVVER AGGCAAGCGAATAAAGAGCTTTTGCTTGAGAGACTTGAGACTCATAYKDDVTAWDVVNEVVD TCAAAACTGTGGTCGAAAGGTACAAGGATGACGTTACGGCCTGGGADGTPNERGLRESVWYQ TGTGGTGAATGAGGTTGTGGACGATGGCACCCCAAATGAAAGGGGAITGDEYIRVAFETARK CTGCGCGAGAGCGTTTGGTATCAGATTACAGGCGATGAATACATTAYAGEDAKLFINDYNTE GAGTGGCATTCGAGACTGCGCGCAAGTACGCTGGCGAAGACGCTAAVTPKRDHLYNLVQDLL GCTGTTCATCAACGACTACAACACGGAGGTGACACCCAAGCGCGATADGVPIDGVGHQAHIQ CACCTCTACAACTTGGTTCAAGACCTGCTCGCGGACGGGGTCCCGAIDWPTIDEIRTSMEMF TCGATGGAGTGGGACATCAAGCCCATATCCAGATCGATTGGCCCACAGLGLDNQVTELDVSL CATCGATGAGATCAGGACCTCGATGGAGATGTTTGCCGGCCTTGGGYGWPPRPAFPTYDAIP CTCGACAACCAAGTTACCGAACTCGATGTTTCCTTGTACGGTTGGCQERFQAQADRYNQLFE CGCCTCGCCCGGCATTCCCGACCTACGATGCAATCCCTCAAGAGAGLYEELDADLSSVTFWG GTTTCAGGCGCAGGCGGATAGATACAATCAGCTCTTCGAGCTTTACIADNHTWLDDRAREYN GAGGAACTCGACGCTGACCTCTCAAGCGTGACCTTCTGGGGGATCGDGVGKDAPFVFDPNYR CGGACAACCATACCTGGCTCGACGACAGGGCCAGAGAATACAATGAVKPAFWRIID* (SEQ CGGGGTCGGCAAAGATGCCCCGTTCGTCTTCGATCCGAACTACAGG ID NO:55) GTTAAACCTGCCTTCTGGCGCATCATTGACTGA (SEQ ID NO: 127) 2027GluB4SP:P40942 MATIAFSRLSIYFCVLATGGCCACCATCGCTTTCTCCCGCTTGTCCATCTACTTCTGCGTGC LLCHGSMAFNDQTSAETTCTCCTGTGCCACGGCTCCATGGCCTTCAACGACCAGACAAGTGC DIPSLAEAFRDYFPIGAGAGGATATTCCGTCACTTGCCGAAGCGTTCAGGGACTATTTCCCT AAIEPGYTTGQIAELYATCGGAGCTGCCATTGAGCCGGGCTATACCACGGGTCAGATTGCCG KKHVNMLVAENAMKPAAATTGTACAAGAAACACGTGAATATGCTGGTCGCGGAGAACGCTAT SLQPTEGNFQWADADRGAAGCCCGCCTCGCTCCAGCCGACGGAGGGTAATTTTCAGTGGGCC IVQFAKENGMELRFHTGACGCGGACCGCATTGTTCAGTTCGCTAAGGAAAACGGAATGGAGC LVWHNQTPTGFSLDKETTCGGTTTCACACGTTGGTGTGGCACAATCAAACCCCAACTGGCTT GKPMVEETDPQKREENCAGCCTGGATAAGGAAGGGAAACCTATGGTCGAGGAAACGGACCCT RKLLLQRLENYIRAVVCAAAAGAGAGAAGAGAACAGGAAACTCCTTTTGCAGCGCCTCGAAA LRYKDDIKSWDVVNEVACTATATCCGGGCCGTTGTGTTGAGATACAAGGATGACATCAAGTC IEPNDPGGMRNSPWYQCTGGGATGTTGTCAATGAGGTTATAGAACCAAACGACCCAGGGGGT ITGTEYIEVAFRATREATGCGTAATTCTCCCTGGTATCAAATCACAGGAACCGAATATATTG AGGSDIKLYINDYNTDAGGTCGCATTTCGCGCGACACGTGAAGCTGGCGGGTCAGATATAAA DPVKRDILYELVKNLLGCTGTATATTAATGATTACAATACGGACGATCCTGTTAAACGGGAT EKGVPIDGVGHQTHIDATACTCTACGAGCTTGTGAAGAACTTGCTGGAGAAAGGTGTCCCGA IYNPPVERIIESIKKFTTGATGGCGTGGGACATCAGACACATATCGACATCTACAACCCACC AGLGLDNIITELDMSICGTTGAAAGGATTATCGAGTCGATTAAGAAGTTCGCCGGACTCGGG YSWNDRSDYGDSIPDYCTTGATAATATCATTACCGAACTGGACATGAGCATCTATTCCTGGA ILTLQAKRYQELFDALATGATCGCTCTGACTACGGTGATTCAATCCCTGACTATATTCTCAC KENKDIVSAVVFWGISCTTGCAGGCCAAAAGATACCAGGAGCTTTTCGATGCGCTGAAGGAG DKYSWLNGFPVKRTNAAATAAGGATATAGTCTCGGCTGTGGTCTTTTGGGGAATTAGCGACA PLLFDRNFMPKPAFWAAATACTCCTGGCTGAATGGCTTCCCGGTCAAGAGGACTAATGCCCC IVDPSRLRE* (SEQATTGCTGTTTGATCGCAACTTTATGCCTAAACCAGCATTTTGGGCA ID NO: 56)ATCGTGGACCCGAGTAGACTCAGGGAATAA (SEQ ID NO: 128) 2028 PR1a:P77853T134-MGFVLFSQLPSFLLVS ATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT 195TLLLFLVISHSCRAQQ CCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCCATSITLTSNASGTFDGY GCAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTGACYYELWKDTGNTTMTVY GGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAATGATQGRFSCQWSNINNAL CGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCGAACATCAAFRTGKKYNQNWQSLGT TAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTGGCAGIRITYSATYNPNGNSY TCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAACCCAALCIYGWSTNPLVEFYI ACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACCCATTVESWGNWRPPGACLAE GGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACCGCCTGSLVLDAATGQRVPIE GGTGCCTGCCTGGCCGAGGGCTCGCTCGTCTTGGACGCGGCTACCGKVRPGMEVFSLGPDYR GGCAGAGGGTCCCTATCGAAAAGGTGCGTCCGGGGATGGAAGTTTTLYRVPVLEVLESGVRE CTCCTTGGGACCTGATTACAGACTGTATCGGGTGCCCGTTTTGGAGVVRLRTRSGRTLVLTP GTCCTTGAGAGCGGGGTTAGGGAAGTTGTGCGCCTCAGAACTCGGTDHPLLTPEGWKPLCDL CAGGGAGAACGCTGGTGTTGACACCAGATCACCCGCTTTTGACCCCPLGTPIAVPAELPVAG CGAAGGTTGGAAACCTCTTTGTGACCTCCCGCTTGGAACTCCAATTHLAPPEERVTLLALLL GCAGTCCCCGCAGAACTGCCTGTGGCGGGCCACTTGGCCCCACCTGGDGNTKLSGRRGTRPN AAGAACGTGTTACGCTCCTGGCTCTTCTGTTGGGGGATGGGAACACASFYSKDPELLAAYRR AAAGCTGTCGGGTCGGAGAGGTACACGTCCTAATGCCTCCTTCTACCAEALGAKVKAYVHPT AGCAAAGACCCCGAATTGCTCGCGGCTTATCGCCGGTGTGCAGAAGTGVVTLATLAPRPGAQ CCTTGGGTGCAAAGGTGAAAGCATACGTCCACCCGACTACGGGGGTDPVKRLVVEAGMVAKA GGTTACACTCGCAACCCTCGCTCCACGTCCTGGAGCTCAAGATCCTEEKRVPEEVFRYRREA GTCAAACGCCTCGTTGTCGAGGCGGGAATGGTTGCTAAAGCCGAAGLALFLGRLFSTDGSVE AGAAGAGGGTCCCGGAGGAGGTGTTTCGTTACCGGCGTGAGGCGTTKKRISYSSASLGLAQD GGCCCTTTTCTTGGGCCGTTTGTTCTCGACAGACGGCTCTGTTGAAVAHLLLRLGIRSQLRS AAGAAGAGGATCTCTTATTCAAGTGCCAGTTTGGGACTGGCCCAGGRGPRAHEVLISGREDI ATGTCGCACATCTCTTGCTGCGCCTTGGAATTAGATCTCAACTCCGLRFAELIGPYLLGAKR TTCGAGAGGGCCACGGGCTCACGAGGTTCTTATATCGGGCCGCGAGERLAALEAEARRRLPG GATATTTTGCGATTTGCTGAACTTATCGGACCCTACCTCTTGGGGGQGWHLRLVLPAVAYRV CCAAGAGGGAGAGACTTGCAGCGCTGGAAGCTGAGGCCCGCAGGCGSEAKRRSGFSWSEAGR TTTGCCTGGACAGGGATGGCACTTGCGGCTTGTTCTTCCTGCCGTGRVAVAGSCLSSGLNLK GCGTACAGAGTGAGCGAGGCTAAAAGGCGCTCGGGATTTTCGTGGALPRRYLSRHRLSLLGE GTGAAGCCGGTCGGCGCGTCGCAGTTGCGGGATCGTGTTTGTCATCAFADPGLEALAEGQVL TGGACTCAACCTCAAATTGCCCAGACGCTACCTTTCTCGGCACCGGWDPIVAVEPAGKARTF TTGTCGCTGCTCGGTGAGGCTTTTGCCGACCCTGGGCTGGAAGCGCDLRVPPFANFVSEDLV TCGCGGAAGGCCAAGTGCTCTGGGACCCTATTGTTGCTGTCGAACCVHNTSLGQVTIDGGTY GGCCGGTAAGGCGAGAACATTCGACTTGCGCGTTCCACCCTTTGCADIYRTTRVNQPSIVGT AACTTCGTGAGCGAGGACCTGGTGGTGCATAACACGTCCCTGGGCCATFDQYWSVRTSKRTS AAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGACACGGTVTVTDHFRAWANRG CGTCAACCAGCCTTCCATTGTGGGGACAGCCACGTTCGATCAGTACLNLGTIDQITLCVEGY TGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAACAGTGACCGTGAQSSGSANITQNTFSQG CCGATCACTTCCGCGCCTGGGCGAACCGGGGCCTGAACCTCGGCACSSSGSSGGSSGSTTTT AATAGACCAAATTACATTGTGCGTGGAGGGTTACCAAAGCTCTGGARIECENMSLSGPYVSR TCAGCCAACATCACCCAGAACACCTTCTCTCAGGGCTCTTCTTCCGITNPFNGIALYANGDT GCAGTTCGGGTGGCTCATCCGGCTCCACAACGACTACTCGCATCGAARATVNFPASRNYNFR GTGTGAGAACATGTCCTTGTCCGGACCCTACGTTAGCAGGATCACCLRGCGNNNNLARVDLR AATCCCTTTAATGGTATTGCGCTGTACGCCAACGGAGACACAGCCCIDGRTVGTFYYQGTYP GCGCTACCGTTAACTTCCCCGCAAGTCGCAACTACAATTTCCGCCTWEAPIDNVYVSAGSHT GCGGGGTTGCGGCAACAACAATAATCTTGCCCGTGTGGACCTGAGGVEITVTADNGTWDVYA ATCGACGGACGGACCGTCGGGACCTTTTATTACCAGGGCACATACC DYLVIQ*(SEQ ID CCTGGGAGGCCCCAATTGACAATGTTTATGTCAGTGCGGGGAGTCA NO: 57)TACAGTCGAAATCACTGTTACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACAGTGA (SEQ ID NO: 129) 2029 BAASS:P77853T134-MANKHLSLSLFLVLLG ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG 195LSASLASGQQTSITLT GCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCTSNASGTFDGYYYELWK GACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTCDTGNTTMTVYTQGRFS TGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTCCQWSNINNALFRTGKK GCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGACYNQNWQSLGTIRITYS CGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGGATYNPNGNSYLCIYGW ATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGTSTNPLVEFYIVESWGN GTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGTWRPPGACLAEGSLVLD TGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCTGCCTGGCCGAGAATGQRVPIEKVRPGM GGCTCGCTCGTCTTGGACGCGGCTACCGGGCAGAGGGTCCCTATCGEVFSLGPDYRLYRVPV AAAAGGTGCGTCCGGGGATGGAAGTTTTCTCCTTGGGACCTGATTALEVLESGVREVVRLRT CAGACTGTATCGGGTGCCCGTTTTGGAGGTCCTTGAGAGCGGGGTTRSGRTLVLTPDHPLLT AGGGAAGTTGTGCGCCTCAGAACTCGGTCAGGGAGAACGCTGGTGTPEGWKPLCDLPLGTPI TGACACCAGATCACCCGCTTTTGACCCCCGAAGGTTGGAAACCTCTAVPAELPVAGHLAPPE TTGTGACCTCCCGCTTGGAACTCCAATTGCAGTCCCCGCAGAACTGERVTLLALLLGDGNTK CCTGTGGCGGGCCACTTGGCCCCACCTGAAGAACGTGTTACGCTCCLSGRRGTRPNASFYSK TGGCTCTTCTGTTGGGGGATGGGAACACAAAGCTGTCGGGTCGGAGDPELLAAYRRCAEALG AGGTACACGTCCTAATGCCTCCTTCTACAGCAAAGACCCCGAATTGAKVKAYVHPTTGVVTL CTCGCGGCTTATCGCCGGTGTGCAGAAGCCTTGGGTGCAAAGGTGAATLAPRPGAQDPVKRL AAGCATACGTCCACCCGACTACGGGGGTGGTTACACTCGCAACCCTVVEAGMVAKAEEKRVP CGCTCCACGTCCTGGAGCTCAAGATCCTGTCAAACGCCTCGTTGTCEEVFRYRREALALFLG GAGGCGGGAATGGTTGCTAAAGCCGAAGAGAAGAGGGTCCCGGAGGRLFSTDGSVEKKRISY AGGTGTTTCGTTACCGGCGTGAGGCGTTGGCCCTTTTCTTGGGCCGSSASLGLAQDVAHLLL TTTGTTCTCGACAGACGGCTCTGTTGAAAAGAAGAGGATCTCTTATRLGIRSQLRSRGPRAH TCAAGTGCCAGTTTGGGACTGGCCCAGGATGTCGCACATCTCTTGCEVLISGREDILRFAEL TGCGCCTTGGAATTAGATCTCAACTCCGTTCGAGAGGGCCACGGGCIGPYLLGAKRERLAAL TCACGAGGTTCTTATATCGGGCCGCGAGGATATTTTGCGATTTGCTEAEARRRLPGQGWHLR GAACTTATCGGACCCTACCTCTTGGGGGCCAAGAGGGAGAGACTTGLVLPAVAYRVSEAKRR CAGCGCTGGAAGCTGAGGCCCGCAGGCGTTTGCCTGGACAGGGATGSGFSWSEAGRRVAVAG GCACTTGCGGCTTGTTCTTCCTGCCGTGGCGTACAGAGTGAGCGAGSCLSSGLNLKLPRRYL GCTAAAAGGCGCTCGGGATTTTCGTGGAGTGAAGCCGGTCGGCGCGSRHRLSLLGEAFADPG TCGCAGTTGCGGGATCGTGTTTGTCATCTGGACTCAACCTCAAATTLEALAEGQVLWDPIVA GCCCAGACGCTACCTTTCTCGGCACCGGTTGTCGCTGCTCGGTGAGVEPAGKARTFDLRVPP GCTTTTGCCGACCCTGGGCTGGAAGCGCTCGCGGAAGGCCAAGTGCFANFVSEDLVVHNTSL TCTGGGACCCTATTGTTGCTGTCGAACCGGCCGGTAAGGCGAGAACGQVTIDGGTYDIYRTT ATTCGACTTGCGCGTTCCACCCTTTGCAAACTTCGTGAGCGAGGACRVNQPSIVGTATFDQY CTGGTGGTGCATAACACGTCCCTGGGCCAAGTGACAATCGATGGCGWSVRTSKRTSGTVTVT GGACCTACGACATCTATAGGACGACACGCGTCAACCAGCCTTCCATDHFRAWANRGLNLGTI TGTGGGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCTDQITLCVEGYQSSGSA AAGCGGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCTNITQNTFSQGSSSGSS GGGCGAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTACATTGGSSGSTTTTRIECEN GTGCGTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAGMSLSGPYVSRITNPFN AACACCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCATGIALYANGDTARATVN CCGGCTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTCCTTFPASRNYNFRLRGCGN GTCCGGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATTNNNLARVDLRIDGRTV GCGCTGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCCGTFYYQGTYPWEAPID CCGCAAGTCGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAANVYVSAGSHTVEITVT CAATAATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTCADNGTWDVYADYLVIQ GGGACCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAATTG * (SEQID NO: ACAATGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGT 58)TACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTG ATACAGTGA (SEQ ID NO:130) 2030 P77853m3 MQTSITLTSNASGTFDATGCAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTG GYYYELWKDTGNTTMTACGGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAAT VYTQGRFSCQWSNLPEGACGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCTAATTTG EWVPLTKNGKSKTFRICCAGAAGAGTGGGTTCCTTTAACTAAGAATGGTAAGTCAAAGACCT GGFVDGLMKANQGKVKTTAGAATTGGAGGCTTCGTAGACGGTTTGATGAAGGCTAACCAAGG KTGDTEVLEVAGIHANAAAGGTCAAGAAGACCGGTGACACCGAAGTATTAGAGGTTGCAGGT SFDRKSKKSRTMAVKAATCCATGCCAATTCCTTTGACAGAAAGTCAAAGAAGTCCAGAACCA VIRHRYSGNVYRIVLNTGGCTGTAAAAGCAGTCATTAGACACAGATATTCCGGAAACGTGTA SGRKITITEGHSLFVYCAGAATAGTTTTGAACTCCGGAAGAAAGATCACCATTACTGAGGGA RNGDLVEATGEDVKIGCATTCCTTATTCGTCTATAGAAACGGTGACTTGGTGGAAGCCACAG DNLAVPRSDGSGDITEGTGAGGATGTAAAGATAGGTGATAACTTAGCTGTTCCAAGAAGCGA DRVVEIKREYYDGYVYCGGATCCGGAGACATTACTGAGGATAGAGTTGTAGAAATTAAGAGA DLSLDEDENFLAGHGYGAGTACTACGACGGTTATGTCTATGACTTGTCATTGGATGAAGATG LMAHNSNINNALFRTGAAAATTTCTTGGCAGGACACGGTTACTTGATGGCCCATAACTCGAA KKYNQNWQSLGTIRITCATCAATAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAAT YSATYNPNGNSYLCIYTGGCAGTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACA GWSTNPLVEFYIVESWACCCAAACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAA GNWRPPGATSLGQVTICCCATTGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGA DGGTYDIYRTTRVNQPCCGCCTGGTGCCACGTCCCTGGGCCAAGTGACAATCGATGGCGGGA SIVGTATFDQYWSVRTCCTACGACATCTATAGGACGACACGCGTCAACCAGCCTTCCATTGT SKRTSGTVTVTDHFRAGGGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCTAAG WANRGLNLGTIDQITLCGGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCTGGG CVEGYQSSGSANITQNCGAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTACATTGTG TFSQGSSSGSSGGSSGCGTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAGAAC STTTTRIECENMSLSGACCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCATCCG PYVSRITNPFNGIALYGCTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTCCTTGTC ANGDTARATVNFPASCCGGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATTGCG NYNFRLRGCGNNNNLACTGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCCCCG RVDLRIDGRTVGTFYYCAAGTTGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAACAA QGTYPWEAPIDNVYVSTAATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTCGGG AGSHTVEITVTADNGTACCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAATTGACA WDVYADYLVIQ*ATGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGTTAC (SEQ ID NO: 59)TGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATA CAGTGA (SEQ ID NO: 131)2031 GluB4SP:P54583 MATIAFSRLSIYFCVLATGGCCACCATCGCTTTCTCCCGCTTGTCCATCTACTTCTGCGTGC LLCHGSMAAGGGYWHTTTCTCCTGTGCCACGGCTCCATGGCCGCTGGAGGAGGATACTGGCA SGREILDANNVPVRIACACTTCCGGCAGGGAGATCCTCGACGCAAATAACGTTCCAGTCAGA GINWFGFETCNYVVHGATCGCCGGGATTAATTGGTTTGGCTTCGAAACGTGTAACTACGTGG LWSRDYRSMLDQIKSLTTCACGGCCTGTGGTCTCGGGATTACAGATCAATGCTCGACCAGAT GYNTIRLPYSDDILKPCAAATCCTTGGGGTATAATACAATTAGGCTGCCCTACAGCGATGAC GTMPNSINFYQMNQDLATTCTTAAGCCTGGAACCATGCCGAACTCGATTAATTTCTACCAAA QGLTSLQVMDKIVAYATGAACCAGGATCTGCAGGGATTGACTTCTCTGCAGGTTATGGACAA GQIGLRIILDRHRPDCGATCGTGGCGTACGCCGGCCAAATCGGGCTCAGAATTATTTTGGAT SGQSALWYTSSVSEATCGGCACAGGCCAGACTGCTCAGGTCAGTCGGCCCTGTGGTACACAA WISDLQALAQRYKGNPGCTCCGTGTCAGAGGCAACATGGATTTCAGATCTTCAAGCCCTCGC TVVGFDLHNEPHDPACACAACGCTATAAAGGCAACCCCACGGTTGTGGGATTCGACCTTCAC WGCGDPSIDWRLAAERAACGAACCTCACGATCCGGCCTGTTGGGGCTGCGGGGACCCTTCGA AGNAVLSVNPNLLIFVTCGACTGGAGACTGGCAGCGGAGAGGGCTGGTAACGCCGTTCTCAG EGVQSYNGDSYWWGGNCGTCAATCCCAACTTGCTGATCTTTGTGGAGGGAGTTCAGTCCTAC LQGAGQYPVVLNVPNRAACGGCGATTCTTACTGGTGGGGCGGAAATCTCCAAGGCGCAGGGC LVYSAHDYATSVYPQTAGTATCCTGTCGTGCTTAACGTTCCGAATCGCCTGGTCTACTCAGC WFSDPTFPNNMPGIWNACACGACTACGCGACTAGCGTGTACCCACAGACGTGGTTCTCCGAT KNWGYLFNQNIAPVWLCCCACATTTCCTAACAATATGCCGGGAATCTGGAACAAGAATTGGG GEFGTTLQSTTDQTWLGTTACTTGTTTAACCAAAACATTGCTCCAGTTTGGTTGGGTGAATT KTLVQYLRPTAQYGADTGGCACCACTCTTCAGTCGACGACAGACCAAACCTGGCTGAAAACC SFQWTFWSWNPDSGDTCTCGTCCAGTATTTGCGGCCAACTGCTCAGTACGGAGCAGATTCTT GGILKDDWQTVDTVKDTTCAATGGACGTTCTGGTCTTGGAATCCTGACTCCGGGGATACAGG GYLAPIKSSIFDPVGACGGTATCCTGAAAGACGATTGGCAGACCGTGGACACTGTTAAGGAC SASPSSQPSPSVSPSPGGGTACTTGGCGCCGATTAAAAGCTCGATCTTTGACCCAGTCGGCG SPSPSASRTPTPTPTPCTAGCGCTTCCCCATCTTCACAACCTTCGCCGAGCGTCAGCCCCAG TASPTPTLTPTATPTPCCCAAGCCCAAGCCCGTCTGCCAGCAGAACCCCCACTCCCACACCT TASPTPSPTAASGARCACCCCCACGGCCTCACCAACTCCGACGCTCACTCCTACGGCGACGC TASYQVNSDWGNGFTVCAACACCAACTGCTTCACCCACTCCTAGCCCCACCGCAGCGAGCGG TVAVTNSGSVATKTWTGGCTAGGTGCACCGCTTCTTACCAGGTCAACTCTGACTGGGGTAAT VSWTFGGNQTITNSWNGGCTTCACCGTGACTGTGGCGGTCACTAACTCAGGAAGCGTCGCGA AAVTQNGQSVTARNMSCGAAAACCTGGACTGTGTCCTGGACGTTCGGGGGCAACCAAACAAT YNNVIQPGQNTTFGFQCACCAACAGCTGGAACGCTGCAGTTACGCAGAATGGGCAAAGCGTC ASYTGSNAAPTVACAAACGGCGCGCAATATGAGCTACAACAACGTGATTCAACCAGGCCAGA S* (SEQ ID NO:ATACCACATTCGGTTTTCAAGCAAGCTATACCGGGTCAAACGCTGC 60)CCCAACTGTCGCTTGTGCTGCCTCATGA (SEQ ID NO: 132) 2032 GluB4SP:P54583:MATIAFSRLSIYFCVL ATGGCCACCATCGCTTTCTCCCGCTTGTCCATCTACTTCTGCGTGC SEKDELLLCHGSMAAGGGYWHT TTCTCCTGTGCCACGGCTCCATGGCCGCTGGAGGAGGATACTGGCASGREILDANNVPVRIA CACTTCCGGCAGGGAGATCCTCGACGCAAATAACGTTCCAGTCAGAGINWFGFETCNYVVHG ATCGCCGGGATTAATTGGTTTGGCTTCGAAACGTGTAACTACGTGGLWSRDYRSMLDQIKSL TTCACGGCCTGTGGTCTCGGGATTACAGATCAATGCTCGACCAGATGYNTIRLPYSDDILKP CAAATCCTTGGGGTATAATACAATTAGGCTGCCCTACAGCGATGACGTMPNSINFYQMNQDL ATTCTTAAGCCTGGAACCATGCCGAACTCGATTAATTTCTACCAAAQGLTSLQVMDKIVAYA TGAACCAGGATCTGCAGGGATTGACTTCTCTGCAGGTTATGGACAAGQIGLRIILDRHRPDC GATCGTGGCGTACGCCGGCCAAATCGGGCTCAGAATTATTTTGGATSGQSALWYTSSVSEAT CGGCACAGGCCAGACTGCTCAGGTCAGTCGGCCCTGTGGTACACAAWISDLQALAQRYKGNP GCTCCGTGTCAGAGGCAACATGGATTTCAGATCTTCAAGCCCTCGCTVVGFDLHNEPHDPAC ACAACGCTATAAAGGCAACCCCACGGTTGTGGGATTCGACCTTCACWGCGDPSIDWRLAAER AACGAACCTCACGATCCGGCCTGTTGGGGCTGCGGGGACCCTTCGAAGNAVLSVNPNLLIFV TCGACTGGAGACTGGCAGCGGAGAGGGCTGGTAACGCCGTTCTCAGEGVQSYNGDSYWWGGN CGTCAATCCCAACTTGCTGATCTTTGTGGAGGGAGTTCAGTCCTACLQGAGQYPVVLNVPNR AACGGCGATTCTTACTGGTGGGGCGGAAATCTCCAAGGCGCAGGGCLVYSAHDYATSVYPQT AGTATCCTGTCGTGCTTAACGTTCCGAATCGCCTGGTCTACTCAGCWFSDPTFPNNMPGIWN ACACGACTACGCGACTAGCGTGTACCCACAGACGTGGTTCTCCGATKNWGYLFNQNIAPVWL CCCACATTTCCTAACAATATGCCGGGAATCTGGAACAAGAATTGGGGEFGTTLQSTTDQTWL GTTACTTGTTTAACCAAAACATTGCTCCAGTTTGGTTGGGTGAATTKTLVQYLRPTAQYGAD TGGCACCACTCTTCAGTCGACGACAGACCAAACCTGGCTGAAAACCSFQWTFWSWNPDSGDT CTCGTCCAGTATTTGCGGCCAACTGCTCAGTACGGAGCAGATTCTTGGILKDDWQTVDTVKD TTCAATGGACGTTCTGGTCTTGGAATCCTGACTCCGGGGATACAGGGYLAPIKSSIFDPVGA CGGTATCCTGAAAGACGATTGGCAGACCGTGGACACTGTTAAGGACSASPSSQPSPSVSPSP GGGTACTTGGCGCCGATTAAAAGCTCGATCTTTGACCCAGTCGGCGSPSPSASRTPTPTPTP CTAGCGCTTCCCCATCTTCACAACCTTCGCCGAGCGTCAGCCCCAGTASPTPTLTPTATPTP CCCAAGCCCAAGCCCGTCTGCCAGCAGAACCCCCACTCCCACACCTTASPTPSPTAASGARC ACCCCCACGGCCTCACCAACTCCGACGCTCACTCCTACGGCGACGCTASYQVNSDWGNGFTV CAACACCAACTGCTTCACCCACTCCTAGCCCCACCGCAGCGAGCGGTVAVTNSGSVATKTWT GGCTAGGTGCACCGCTTCTTACCAGGTCAACTCTGACTGGGGTAATVSWTFGGNQTITNSWN GGCTTCACCGTGACTGTGGCGGTCACTAACTCAGGAAGCGTCGCGAAAVTQNGQSVTARNMS CGAAAACCTGGACTGTGTCCTGGACGTTCGGGGGCAACCAAACAATYNNVIQPGQNTTFGFQ CACCAACAGCTGGAACGCTGCAGTTACGCAGAATGGGCAAAGCGTCASYTGSNAAPTVACAA ACGGCGCGCAATATGAGCTACAACAACGTGATTCAACCAGGCCAGA SSEKDEL*(SEQ ID ATACCACATTCGGTTTTCAAGCAAGCTATACCGGGTCAAACGCTGC NO: 61)CCCAACTGTCGCTTGTGCTGCCTCAAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 133) 2033P54583 MAGGGYWHTSGREILD ATGGCTGGAGGAGGATACTGGCACACTTCCGGCAGGGAGATCCTCGANNVPVRIAGINWFGF ACGCAAATAACGTTCCAGTCAGAATCGCCGGGATTAATTGGTTTGGETCNYVVHGLWSRDYR CTTCGAAACGTGTAACTACGTGGTTCACGGCCTGTGGTCTCGGGATSMLDQIKSLGYNTIRL TACAGATCAATGCTCGACCAGATCAAATCCTTGGGGTATAATACAAPYSDDILKPGTMPNSI TTAGGCTGCCCTACAGCGATGACATTCTTAAGCCTGGAACCATGCCNFYQMNQDLQGLTSLQ GAACTCGATTAATTTCTACCAAATGAACCAGGATCTGCAGGGATTGVMDKIVAYAGQIGLRI ACTTCTCTGCAGGTTATGGACAAGATCGTGGCGTACGCCGGCCAAAILDRHRPDCSGQSALW TCGGGCTCAGAATTATTTTGGATCGGCACAGGCCAGACTGCTCAGGYTSSVSEATWISDLQA TCAGTCGGCCCTGTGGTACACAAGCTCCGTGTCAGAGGCAACATGGLAQRYKGNPTVVGFDL ATTTCAGATCTTCAAGCCCTCGCACAACGCTATAAAGGCAACCCCAHNEPHDPACWGCGDPS CGGTTGTGGGATTCGACCTTCACAACGAACCTCACGATCCGGCCTGIDWRLAAERAGNAVLS TTGGGGCTGCGGGGACCCTTCGATCGACTGGAGACTGGCAGCGGAGVNPNLLIFVEGVQSYN AGGGCTGGTAACGCCGTTCTCAGCGTCAATCCCAACTTGCTGATCTGDSYWWGGNLQGAGQY TTGTGGAGGGAGTTCAGTCCTACAACGGCGATTCTTACTGGTGGGGPVVLNVPNRLVYSAHD CGGAAATCTCCAAGGCGCAGGGCAGTATCCTGTCGTGCTTAACGTTYATSVYPQTWFSDPTF CCGAATCGCCTGGTCTACTCAGCACACGACTACGCGACTAGCGTGTPNNMPGIWNKNWGYLF ACCCACAGACGTGGTTCTCCGATCCCACATTTCCTAACAATATGCCNQNIAPVWLGEFGTTL GGGAATCTGGAACAAGAATTGGGGTTACTTGTTTAACCAAAACATTQSTTDQTWLKTLVQYL GCTCCAGTTTGGTTGGGTGAATTTGGCACCACTCTTCAGTCGACGARPTAQYGADSFQWTFW CAGACCAAACCTGGCTGAAAACCCTCGTCCAGTATTTGCGGCCAACSWNPDSGDTGGILKDD TGCTCAGTACGGAGCAGATTCTTTTCAATGGACGTTCTGGTCTTGGWQTVDTVKDGYLAPIK AATCCTGACTCCGGGGATACAGGCGGTATCCTGAAAGACGATTGGCSSIFDPVGASASPSSQ AGACCGTGGACACTGTTAAGGACGGGTACTTGGCGCCGATTAAAAGPSPSVSPSPSPSPSAS CTCGATCTTTGACCCAGTCGGCGCTAGCGCTTCCCCATCTTCACAARTPTPTPTPTASPTPT CCTTCGCCGAGCGTCAGCCCCAGCCCAAGCCCAAGCCCGTCTGCCALTPTATPTPTASPTPS GCAGAACCCCCACTCCCACACCTACCCCCACGGCCTCACCAACTCCPTAASGARCTASYQVN GACGCTCACTCCTACGGCGACGCCAACACCAACTGCTTCACCCACTSDWGNGFTVTVAVTNS CCTAGCCCCACCGCAGCGAGCGGGGCTAGGTGCACCGCTTCTTACCGSVATKTWTVSWTFGG AGGTCAACTCTGACTGGGGTAATGGCTTCACCGTGACTGTGGCGGTNQTITNSWNAAVTQNG CACTAACTCAGGAAGCGTCGCGACGAAAACCTGGACTGTGTCCTGGQSVTARNMSYNNVIQP ACGTTCGGGGGCAACCAAACAATCACCAACAGCTGGAACGCTGCAGGQNTTFGFQASYTGSN TTACGCAGAATGGGCAAAGCGTCACGGCGCGCAATATGAGCTACAAAAPTVACAAS* CAACGTGATTCAACCAGGCCAGAATACCACATTCGGTTTTCAAGCA (SEQ ID NO:62) AGCTATACCGGGTCAAACGCTGCCCCAACTGTCGCTTGTGCTGCCT CATGA (SEQ ID NO:134) 2034 P54583:SEKDEL MAGGGYWHTSGREILDATGGCTGGAGGAGGATACTGGCACACTTCCGGCAGGGAGATCCTCG ANNVPVRIAGINWFGFACGCAAATAACGTTCCAGTCAGAATCGCCGGGATTAATTGGTTTGG ETCNYVVHGLWSRDYRCTTCGAAACGTGTAACTACGTGGTTCACGGCCTGTGGTCTCGGGAT SMLDQIKSLGYNTIRLTACAGATCAATGCTCGACCAGATCAAATCCTTGGGGTATAATACAA PYSDDILKPGTMPNSITTAGGCTGCCCTACAGCGATGACATTCTTAAGCCTGGAACCATGCC NFYQMNQDLQGLTSLQGAACTCGATTAATTTCTACCAAATGAACCAGGATCTGCAGGGATTG VMDKIVAYAGQIGLRIACTTCTCTGCAGGTTATGGACAAGATCGTGGCGTACGCCGGCCAAA ILDRHRPDCSGQSALWTCGGGCTCAGAATTATTTTGGATCGGCACAGGCCAGACTGCTCAGG YTSSVSEATWISDLQATCAGTCGGCCCTGTGGTACACAAGCTCCGTGTCAGAGGCAACATGG LAQRYKGNPTVVGFDLATTTCAGATCTTCAAGCCCTCGCACAACGCTATAAAGGCAACCCCA HNEPHDPACWGCGDPSCGGTTGTGGGATTCGACCTTCACAACGAACCTCACGATCCGGCCTG IDWRLAAERAGNAVLSTTGGGGCTGCGGGGACCCTTCGATCGACTGGAGACTGGCAGCGGAG VNPNLLIFVEGVQSYNAGGGCTGGTAACGCCGTTCTCAGCGTCAATCCCAACTTGCTGATCT GDSYWWGGNLQGAGQYTTGTGGAGGGAGTTCAGTCCTACAACGGCGATTCTTACTGGTGGGG PVVLNVPNRLVYSAHDCGGAAATCTCCAAGGCGCAGGGCAGTATCCTGTCGTGCTTAACGTT YATSVYPQTWFSDPTFCCGAATCGCCTGGTCTACTCAGCACACGACTACGCGACTAGCGTGT PNNMPGIWNKNWGYLFACCCACAGACGTGGTTCTCCGATCCCACATTTCCTAACAATATGCC NQNIAPVWLGEFGTTLGGGAATCTGGAACAAGAATTGGGGTTACTTGTTTAACCAAAACATT QSTTDQTWLKTLVQYLGCTCCAGTTTGGTTGGGTGAATTTGGCACCACTCTTCAGTCGACGA RPTAQYGADSFQWTFWCAGACCAAACCTGGCTGAAAACCCTCGTCCAGTATTTGCGGCCAAC SWNPDSGDTGGILKDDTGCTCAGTACGGAGCAGATTCTTTTCAATGGACGTTCTGGTCTTGG WQTVDTVKDGYLAPIKAATCCTGACTCCGGGGATACAGGCGGTATCCTGAAAGACGATTGGC SSIFDPVGASASPSSQAGACCGTGGACACTGTTAAGGACGGGTACTTGGCGCCGATTAAAAG PSPSVSPSPSPSPSASCTCGATCTTTGACCCAGTCGGCGCTAGCGCTTCCCCATCTTCACAA RTPTPTPTPTASPTPTCCTTCGCCGAGCGTCAGCCCCAGCCCAAGCCCAAGCCCGTCTGCCA LTPTATPTPTASPTPSGCAGAACCCCCACTCCCACACCTACCCCCACGGCCTCACCAACTCC PTAASGARCTASYQVNGACGCTCACTCCTACGGCGACGCCAACACCAACTGCTTCACCCACT SDWGNGFTVTVAVTNSCCTAGCCCCACCGCAGCGAGCGGGGCTAGGTGCACCGCTTCTTACC GSVATKTWTVSWTFGGAGGTCAACTCTGACTGGGGTAATGGCTTCACCGTGACTGTGGCGGT NQTITNSWNAAVTQNGCACTAACTCAGGAAGCGTCGCGACGAAAACCTGGACTGTGTCCTGG QSVTARNMSYNNVIQPACGTTCGGGGGCAACCAAACAATCACCAACAGCTGGAACGCTGCAG GQNTTFGFQASYTGSNTTACGCAGAATGGGCAAAGCGTCACGGCGCGCAATATGAGCTACAA AAPTVACAASSEKDELCAACGTGATTCAACCAGGCCAGAATACCACATTCGGTTTTCAAGCA * (SEQ ID NO:AGCTATACCGGGTCAAACGCTGCCCCAACTGTCGCTTGTGCTGCCT 63)CAAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 135) 2035 PR1a:P54583MGFVLFSQLPSFLLVS ATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCTTLLLFLVISHSCRAQN CCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCCAAGGGYWHTSGREILDA GAACGCTGGAGGAGGATACTGGCACACTTCCGGCAGGGAGATCCTCNNVPVRIAGINWFGFE GACGCAAATAACGTTCCAGTCAGAATCGCCGGGATTAATTGGTTTGTCNYVVHGLWSRDYRS GCTTCGAAACGTGTAACTACGTGGTTCACGGCCTGTGGTCTCGGGAMLDQIKSLGYNTIRLP TTACAGATCAATGCTCGACCAGATCAAATCCTTGGGGTATAATACAYSDDILKPGTMPNSIN ATTAGGCTGCCCTACAGCGATGACATTCTTAAGCCTGGAACCATGCFYQMNQDLQGLTSLQV CGAACTCGATTAATTTCTACCAAATGAACCAGGATCTGCAGGGATTMDKIVAYAGQIGLRII GACTTCTCTGCAGGTTATGGACAAGATCGTGGCGTACGCCGGCCAALDRHRPDCSGQSALWY ATCGGGCTCAGAATTATTTTGGATCGGCACAGGCCAGACTGCTCAGTSSVSEATWISDLQAL GTCAGTCGGCCCTGTGGTACACAAGCTCCGTGTCAGAGGCAACATGAQRYKGNPTVVGFDLH GATTTCAGATCTTCAAGCCCTCGCACAACGCTATAAAGGCAACCCCNEPHDPACWGCGDPSI ACGGTTGTGGGATTCGACCTTCACAACGAACCTCACGATCCGGCCTDWRLAAERAGNAVLSV GTTGGGGCTGCGGGGACCCTTCGATCGACTGGAGACTGGCAGCGGANPNLLIFVEGVQSYNG GAGGGCTGGTAACGCCGTTCTCAGCGTCAATCCCAACTTGCTGATCDSYWWGGNLQGAGQYP TTTGTGGAGGGAGTTCAGTCCTACAACGGCGATTCTTACTGGTGGGVVLNVPNRLVYSAHDY GCGGAAATCTCCAAGGCGCAGGGCAGTATCCTGTCGTGCTTAACGTATSVYPQTWFSDPTFP TCCGAATCGCCTGGTCTACTCAGCACACGACTACGCGACTAGCGTGNNMPGIWNKNWGYLFN TACCCACAGACGTGGTTCTCCGATCCCACATTTCCTAACAATATGCQNIAPVWLGEFGTTLQ CGGGAATCTGGAACAAGAATTGGGGTTACTTGTTTAACCAAAACATSTTDQTWLKTLVQYLR TGCTCCAGTTTGGTTGGGTGAATTTGGCACCACTCTTCAGTCGACGPTAQYGADSFQWTFWS ACAGACCAAACCTGGCTGAAAACCCTCGTCCAGTATTTGCGGCCAAWNPDSGDTGGILKDDW CTGCTCAGTACGGAGCAGATTCTTTTCAATGGACGTTCTGGTCTTGQTVDTVKDGYLAPIKS GAATCCTGACTCCGGGGATACAGGCGGTATCCTGAAAGACGATTGGSIFDPVGASASPSSQP CAGACCGTGGACACTGTTAAGGACGGGTACTTGGCGCCGATTAAAASPSVSPSPSPSPSASR GCTCGATCTTTGACCCAGTCGGCGCTAGCGCTTCCCCATCTTCACATPTPTPTPTASPTPTL ACCTTCGCCGAGCGTCAGCCCCAGCCCAAGCCCAAGCCCGTCTGCCTPTATPTPTASPTPSP AGCAGAACCCCCACTCCCACACCTACCCCCACGGCCTCACCAACTCTAASGARCTASYQVNS CGACGCTCACTCCTACGGCGACGCCAACACCAACTGCTTCACCCACDWGNGFTVTVAVTNSG TCCTAGCCCCACCGCAGCGAGCGGGGCTAGGTGCACCGCTTCTTACSVATKTWTVSWTFGGN CAGGTCAACTCTGACTGGGGTAATGGCTTCACCGTGACTGTGGCGGQTITNSWNAAVTQNGQ TCACTAACTCAGGAAGCGTCGCGACGAAAACCTGGACTGTGTCCTGSVTARNMSYNNVIQPG GACGTTCGGGGGCAACCAAACAATCACCAACAGCTGGAACGCTGCAQNTTFGFQASYTGSNA GTTACGCAGAATGGGCAAAGCGTCACGGCGCGCAATATGAGCTACAAPTVACAAS* ACAACGTGATTCAACCAGGCCAGAATACCACATTCGGTTTTCAAGC (SEQ ID NO:64) AAGCTATACCGGGTCAAACGCTGCCCCAACTGTCGCTTGTGCTGCC TCATGA (SEQ ID NO:136) 2036 PR1a:P54583:SEKDEL MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT TLLLFLVISHSCRAQNCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCCA AGGGYWHTSGREILDAGAACGCTGGAGGAGGATACTGGCACACTTCCGGCAGGGAGATCCTC NNVPVRIAGINWFGFEGACGCAAATAACGTTCCAGTCAGAATCGCCGGGATTAATTGGTTTG TCNYVVHGLWSRDYRSGCTTCGAAACGTGTAACTACGTGGTTCACGGCCTGTGGTCTCGGGA MLDQIKSLGYNTIRLPTTACAGATCAATGCTCGACCAGATCAAATCCTTGGGGTATAATACA YSDDILKPGTMPNSINATTAGGCTGCCCTACAGCGATGACATTCTTAAGCCTGGAACCATGC FYQMNQDLQGLTSLQVCGAACTCGATTAATTTCTACCAAATGAACCAGGATCTGCAGGGATT MDKIVAYAGQIGLRIIGACTTCTCTGCAGGTTATGGACAAGATCGTGGCGTACGCCGGCCAA LDRHRPDCSGQSALWYATCGGGCTCAGAATTATTTTGGATCGGCACAGGCCAGACTGCTCAG TSSVSEATWISDLQALGTCAGTCGGCCCTGTGGTACACAAGCTCCGTGTCAGAGGCAACATG AQRYKGNPTVVGFDLHGATTTCAGATCTTCAAGCCCTCGCACAACGCTATAAAGGCAACCCC NEPHDPACWGCGDPSIACGGTTGTGGGATTCGACCTTCACAACGAACCTCACGATCCGGCCT DWRLAAERAGNAVLSVGTTGGGGCTGCGGGGACCCTTCGATCGACTGGAGACTGGCAGCGGA NPNLLIFVEGVQSYNGGAGGGCTGGTAACGCCGTTCTCAGCGTCAATCCCAACTTGCTGATC DSYWWGGNLQGAGQYPTTTGTGGAGGGAGTTCAGTCCTACAACGGCGATTCTTACTGGTGGG VVLNVPNRLVYSAHDYGCGGAAATCTCCAAGGCGCAGGGCAGTATCCTGTCGTGCTTAACGT ATSVYPQTWFSDPTFPTCCGAATCGCCTGGTCTACTCAGCACACGACTACGCGACTAGCGTG NNMPGIWNKNWGYLFNTACCCACAGACGTGGTTCTCCGATCCCACATTTCCTAACAATATGC QNIAPVWLGEFGTTLQCGGGAATCTGGAACAAGAATTGGGGTTACTTGTTTAACCAAAACAT STTDQTWLKTLVQYLRTGCTCCAGTTTGGTTGGGTGAATTTGGCACCACTCTTCAGTCGACG PTAQYGADSFQWTFWSACAGACCAAACCTGGCTGAAAACCCTCGTCCAGTATTTGCGGCCAA WNPDSGDTGGILKDDWCTGCTCAGTACGGAGCAGATTCTTTTCAATGGACGTTCTGGTCTTG QTVDTVKDGYLAPIKSGAATCCTGACTCCGGGGATACAGGCGGTATCCTGAAAGACGATTGG SIFDPVGASASPSSQPCAGACCGTGGACACTGTTAAGGACGGGTACTTGGCGCCGATTAAAA SPSVSPSPSPSPSASRGCTCGATCTTTGACCCAGTCGGCGCTAGCGCTTCCCCATCTTCACA TPTPTPTPTASPTPTLACCTTCGCCGAGCGTCAGCCCCAGCCCAAGCCCAAGCCCGTCTGCC TPTATPTPTASPTPSPAGCAGAACCCCCACTCCCACACCTACCCCCACGGCCTCACCAACTC TAASGARCTASYQVNSCGACGCTCACTCCTACGGCGACGCCAACACCAACTGCTTCACCCAC DWGNGFTVTVAVTNSGTCCTAGCCCCACCGCAGCGAGCGGGGCTAGGTGCACCGCTTCTTAC SVATKTWTVSWTFGGNCAGGTCAACTCTGACTGGGGTAATGGCTTCACCGTGACTGTGGCGG QTITNSWNAAVTQNGQTCACTAACTCAGGAAGCGTCGCGACGAAAACCTGGACTGTGTCCTG SVTARNMSYNNVIQPGGACGTTCGGGGGCAACCAAACAATCACCAACAGCTGGAACGCTGCA QNTTFGFQASYTGSNAGTTACGCAGAATGGGCAAAGCGTCACGGCGCGCAATATGAGCTACA APTVACAASSEKDEL*ACAACGTGATTCAACCAGGCCAGAATACCACATTCGGTTTTCAAGC (SEQ ID NO: 65)AAGCTATACCGGGTCAAACGCTGCCCCAACTGTCGCTTGTGCTGCC TCAAGCGAGAAGGACGAGCTGTGA(SEQ ID NO: 137) 2037 BAASS:P54583 MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQVAGGGYWGCCTGTCGGCCAGCTTGGCCTCCGGGCAAGTCGCTGGAGGAGGATA HTSGREILDANNVPVRCTGGCACACTTCCGGCAGGGAGATCCTCGACGCAAATAACGTTCCA IAGINWFGFETCNYVVGTCAGAATCGCCGGGATTAATTGGTTTGGCTTCGAAACGTGTAACT HGLWSRDYRSMLDQIKACGTGGTTCACGGCCTGTGGTCTCGGGATTACAGATCAATGCTCGA SLGYNTIRLPYSDDILCCAGATCAAATCCTTGGGGTATAATACAATTAGGCTGCCCTACAGC KPGTMPNSINFYQMNQGATGACATTCTTAAGCCTGGAACCATGCCGAACTCGATTAATTTCT DLQGLTSLQVMDKIVAACCAAATGAACCAGGATCTGCAGGGATTGACTTCTCTGCAGGTTAT YAGQIGLRIILDRHRPGGACAAGATCGTGGCGTACGCCGGCCAAATCGGGCTCAGAATTATT DCSGQSALWYTSSVSETTGGATCGGCACAGGCCAGACTGCTCAGGTCAGTCGGCCCTGTGGT ATWISDLQALAQRYKGACACAAGCTCCGTGTCAGAGGCAACATGGATTTCAGATCTTCAAGC NPTVVGFDLHNEPHDPCCTCGCACAACGCTATAAAGGCAACCCCACGGTTGTGGGATTCGAC ACWGCGDPSIDWRLAACTTCACAACGAACCTCACGATCCGGCCTGTTGGGGCTGCGGGGACC ERAGNAVLSVNPNLLICTTCGATCGACTGGAGACTGGCAGCGGAGAGGGCTGGTAACGCCGT FVEGVQSYNGDSYWWGTCTCAGCGTCAATCCCAACTTGCTGATCTTTGTGGAGGGAGTTCAG GNLQGAGQYPVVLNVPTCCTACAACGGCGATTCTTACTGGTGGGGCGGAAATCTCCAAGGCG NRLVYSAHDYATSVYPCAGGGCAGTATCCTGTCGTGCTTAACGTTCCGAATCGCCTGGTCTA QTWFSDPTFPNNMPGICTCAGCACACGACTACGCGACTAGCGTGTACCCACAGACGTGGTTC WNKNWGYLFNQNIAPVTCCGATCCCACATTTCCTAACAATATGCCGGGAATCTGGAACAAGA WLGEFGTTLQSTTDQTATTGGGGTTACTTGTTTAACCAAAACATTGCTCCAGTTTGGTTGGG WLKTLVQYLRPTAQYGTGAATTTGGCACCACTCTTCAGTCGACGACAGACCAAACCTGGCTG ADSFQWTFWSWNPDSGAAAACCCTCGTCCAGTATTTGCGGCCAACTGCTCAGTACGGAGCAG DTGGILKDDWQTVDTVATTCTTTTCAATGGACGTTCTGGTCTTGGAATCCTGACTCCGGGGA KDGYLAPIKSSIFDPVTACAGGCGGTATCCTGAAAGACGATTGGCAGACCGTGGACACTGTT GASASPSSQPSPSVSPAAGGACGGGTACTTGGCGCCGATTAAAAGCTCGATCTTTGACCCAG SPSPSPSASRTPTPTPTCGGCGCTAGCGCTTCCCCATCTTCACAACCTTCGCCGAGCGTCAG TPTASPTPTLTPTATPCCCCAGCCCAAGCCCAAGCCCGTCTGCCAGCAGAACCCCCACTCCC TPTASPTPSPTAASGAACACCTACCCCCACGGCCTCACCAACTCCGACGCTCACTCCTACGG RCTASYQVNSDWGNGFCGACGCCAACACCAACTGCTTCACCCACTCCTAGCCCCACCGCAGC TVTVAVTNSGSVATKTGAGCGGGGCTAGGTGCACCGCTTCTTACCAGGTCAACTCTGACTGG WTVSWTFGGNQTITNSGGTAATGGCTTCACCGTGACTGTGGCGGTCACTAACTCAGGAAGCG WNAAVTQNGQSVTARNTCGCGACGAAAACCTGGACTGTGTCCTGGACGTTCGGGGGCAACCA MSYNNVIQPGQNTTFGAACAATCACCAACAGCTGGAACGCTGCAGTTACGCAGAATGGGCAA FQASYTGSNAAPTVACAGCGTCACGGCGCGCAATATGAGCTACAACAACGTGATTCAACCAG AAS* (SEQ IDGCCAGAATACCACATTCGGTTTTCAAGCAAGCTATACCGGGTCAAA NO: 66)CGCTGCCCCAACTGTCGCTTGTGCTGCCTCATGA (SEQ ID NO: 138) 2038BAASS:P54583:SEKDEL MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQVAGGGYWGCCTGTCGGCCAGCTTGGCCTCCGGGCAAGTCGCTGGAGGAGGATA HTSGREILDANNVPVRCTGGCACACTTCCGGCAGGGAGATCCTCGACGCAAATAACGTTCCA IAGINWFGFETCNYVVGTCAGAATCGCCGGGATTAATTGGTTTGGCTTCGAAACGTGTAACT HGLWSRDYRSMLDQIKACGTGGTTCACGGCCTGTGGTCTCGGGATTACAGATCAATGCTCGA SLGYNTIRLPYSDDILCCAGATCAAATCCTTGGGGTATAATACAATTAGGCTGCCCTACAGC KPGTMPNSINFYQMNQGATGACATTCTTAAGCCTGGAACCATGCCGAACTCGATTAATTTCT DLQGLTSLQVMDKIVAACCAAATGAACCAGGATCTGCAGGGATTGACTTCTCTGCAGGTTAT YAGQIGLRIILDRHRPGGACAAGATCGTGGCGTACGCCGGCCAAATCGGGCTCAGAATTATT DCSGQSALWYTSSVSETTGGATCGGCACAGGCCAGACTGCTCAGGTCAGTCGGCCCTGTGGT ATWISDLQALAQRYKGACACAAGCTCCGTGTCAGAGGCAACATGGATTTCAGATCTTCAAGC NPTVVGFDLHNEPHDPCCTCGCACAACGCTATAAAGGCAACCCCACGGTTGTGGGATTCGAC ACWGCGDPSIDWRLAACTTCACAACGAACCTCACGATCCGGCCTGTTGGGGCTGCGGGGACC ERAGNAVLSVNPNLLICTTCGATCGACTGGAGACTGGCAGCGGAGAGGGCTGGTAACGCCGT FVEGVQSYNGDSYWWGTCTCAGCGTCAATCCCAACTTGCTGATCTTTGTGGAGGGAGTTCAG GNLQGAGQYPVVLNVPTCCTACAACGGCGATTCTTACTGGTGGGGCGGAAATCTCCAAGGCG NRLVYSAHDYATSVYPCAGGGCAGTATCCTGTCGTGCTTAACGTTCCGAATCGCCTGGTCTA QTWFSDPTFPNNMPGICTCAGCACACGACTACGCGACTAGCGTGTACCCACAGACGTGGTTC WNKNWGYLFNQNIAPVTCCGATCCCACATTTCCTAACAATATGCCGGGAATCTGGAACAAGA WLGEFGTTLQSTTDQTATTGGGGTTACTTGTTTAACCAAAACATTGCTCCAGTTTGGTTGGG WLKTLVQYLRPTAQYGTGAATTTGGCACCACTCTTCAGTCGACGACAGACCAAACCTGGCTG ADSFQWTFWSWNPDSGAAAACCCTCGTCCAGTATTTGCGGCCAACTGCTCAGTACGGAGCAG DTGGILKDDWQTVDTVATTCTTTTCAATGGACGTTCTGGTCTTGGAATCCTGACTCCGGGGA KDGYLAPIKSSIFDPVTACAGGCGGTATCCTGAAAGACGATTGGCAGACCGTGGACACTGTT GASASPSSQPSPSVSPAAGGACGGGTACTTGGCGCCGATTAAAAGCTCGATCTTTGACCCAG SPSPSPSASRTPTPTPTCGGCGCTAGCGCTTCCCCATCTTCACAACCTTCGCCGAGCGTCAG TPTASPTPTLTPTATPCCCCAGCCCAAGCCCAAGCCCGTCTGCCAGCAGAACCCCCACTCCC TPTASPTPSPTAASGAACACCTACCCCCACGGCCTCACCAACTCCGACGCTCACTCCTACGG RCTASYQVNSDWGNGFCGACGCCAACACCAACTGCTTCACCCACTCCTAGCCCCACCGCAGC TVTVAVTNSGSVATKTGAGCGGGGCTAGGTGCACCGCTTCTTACCAGGTCAACTCTGACTGG WTVSWTFGGNQTITNSGGTAATGGCTTCACCGTGACTGTGGCGGTCACTAACTCAGGAAGCG WNAAVTQNGQSVTARNTCGCGACGAAAACCTGGACTGTGTCCTGGACGTTCGGGGGCAACCA MSYNNVIQPGQNTTFGAACAATCACCAACAGCTGGAACGCTGCAGTTACGCAGAATGGGCAA FQASYTGSNAAPTVACAGCGTCACGGCGCGCAATATGAGCTACAACAACGTGATTCAACCAG AASSEKDEL*GCCAGAATACCACATTCGGTTTTCAAGCAAGCTATACCGGGTCAAA (SEQ ID NO: 67)CGCTGCCCCAACTGTCGCTTGTGCTGCCTCAAGCGAGAAGGACGAG CTGTGA (SEQ ID NO: 139)2040 PR1a:NtEGm MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT TLLLFLVISHSCRAAYCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCGC DYKQVLRDSLLFYEAQTTACGACTACAAGCAGGTGTTGCGGGACTCGCTACTATTCTATGAG RSGRLPADQKVTWRKDGCCCAGAGATCCGGCCGGCTCCCAGCCGACCAGAAGGTCACGTGGA SALNDQGDQGQDLTGGGGAAGGATAGCGCGCTGAATGACCAGGGTGACCAGGGACAAGACTT YFDAGDFVKFGFPMAYGACCGGCGGCTACTTTGACGCTGGGGACTTCGTCAAGTTCGGGTTC TATVLAWGLIDFEAGYCCCATGGCTTATACCGCAACCGTGCTGGCATGGGGCCTCATAGATT SSAGALDDGRKAVKWATTGAGGCCGGCTACAGCAGTGCCGGGGCCTTGGATGATGGACGGAA TDYFIKAHTSQNEFYGGGCTGTCAAATGGGCCACCGACTATTTCATAAAGGCCCACACAAGT QVGQGDADHAFWGRPECAAAATGAGTTCTATGGTCAGGTCGGCCAGGGTGACGCCGATCACG DMTMARPAYKIDTSRPCTTTCTGGGGAAGACCAGAGGATATGACGATGGCGCGCCCGGCGTA GSDLAGETAAALAAASCAAGATAGACACCTCAAGGCCTGGCTCTGATCTGGCAGGCGAGACA IVFRNVDGTYSNNLLTGCGGCTGCTCTTGCCGCTGCTTCAATCGTGTTCCGGAACGTCGATG HARQLFDFANNYRGKYGCACTTACTCAAATAACCTGTTAACACACGCTCGCCAGCTATTCGA SDSITDARNFYASADYCTTCGCGAACAACTACCGGGGAAAGTATAGTGACTCTATTACTGAC RDELVWAAAWLYRATNGCAAGAAATTTCTACGCAAGCGCAGACTACAGAGACGAGTTGGTTT DNTYLNTAESLYDEFGGGGCTGCTGCGTGGTTATACAGAGCGACCAACGACAACACCTACCT LQNWGGGLNWDSKVSGCAACACTGCTGAGTCACTGTACGATGAGTTTGGGCTACAGAACTGG VQVLLAKLTNKQAYKDGGGGGGGGCCTGAACTGGGATAGCAAGGTGTCTGGCGTGCAGGTGT TVQSYVNYLINNQQKTTGTTGGCCAAGCTTACCAATAAGCAGGCCTACAAGGACACGGTGCA PKGLLYIDMWGTLRHAGTCTTACGTCAATTACCTAATTAATAACCAGCAGAAGACTCCCAAG ANAAFIMLEAAELGLSGGCCTCCTCTACATCGACATGTGGGGCACCCTTCGCCACGCTGCCA ASSYRQFAQTQIDYALACGCCGCATTCATCATGCTCGAAGCCGCCGAGCTGGGCTTGTCCGC GDGGRSFVCGFGSNPPCTCCTCTTATAGACAGTTCGCGCAAACGCAAATCGACTACGCCCTG TRPHHRSSSCPPAPATGGCGATGGTGGCCGCTCCTTTGTGTGCGGGTTCGGGAGTAATCCTC CDWNTFNSPDPNYHVLCTACGAGACCGCACCACAGATCCTCGTCGTGCCCGCCAGCTCCCGC SGALVGGPDQNDNYVDTACTTGCGACTGGAATACATTCAACTCACCTGACCCAAACTACCAC DRSDYVHNEVATDYNAGTCCTCTCTGGGGCCCTAGTGGGCGGACCTGATCAGAATGACAACT GFQSALAALVALGY*ACGTCGATGACCGTTCAGACTATGTTCACAACGAAGTCGCCACTGA (SEQ ID NO: 68)TTACAACGCGGGTTTCCAGTCCGCGTTAGCTGCTTTGGTGGCCCTT GGTTACTGA (SEQ ID NO:140) 2041 GluB4SP:NtEGm MATIAFSRLSIYFCVLATGGCCACCATCGCTTTCTCCCGCTTGTCCATCTACTTCTGCGTGC LLCHGSMAAYDYKQVLTTCTCCTGTGCCACGGCTCCATGGCCGCTTACGACTACAAGCAGGT RDSLLFYEAQRSGRLPGTTGCGGGACTCGCTACTATTCTATGAGGCCCAGAGATCCGGCCGG ADQKVTWRKDSALNDQCTCCCAGCCGACCAGAAGGTCACGTGGAGGAAGGATAGCGCGCTGA GDQGQDLTGGYFDAGDATGACCAGGGTGACCAGGGACAAGACTTGACCGGCGGCTACTTTGA FVKFGFPMAYTATVLACGCTGGGGACTTCGTCAAGTTCGGGTTCCCCATGGCTTATACCGCA WGLIDFEAGYSSAGALACCGTGCTGGCATGGGGCCTCATAGATTTTGAGGCCGGCTACAGCA DDGRKAVKWATDYFIKGTGCCGGGGCCTTGGATGATGGACGGAAGGCTGTCAAATGGGCCAC AHTSQNEFYGQVGQGDCGACTATTTCATAAAGGCCCACACAAGTCAAAATGAGTTCTATGGT ADHAFWGRPEDMTMARCAGGTCGGCCAGGGTGACGCCGATCACGCTTTCTGGGGAAGACCAG PAYKIDTSRPGSDLAGAGGATATGACGATGGCGCGCCCGGCGTACAAGATAGACACCTCAAG ETAAALAAASIVFRNVGCCTGGCTCTGATCTGGCAGGCGAGACAGCGGCTGCTCTTGCCGCT DGTYSNNLLTHARQLFGCTTCAATCGTGTTCCGGAACGTCGATGGCACTTACTCAAATAACC DFANNYRGKYSDSITDTGTTAACACACGCTCGCCAGCTATTCGACTTCGCGAACAACTACCG ARNFYASADYRDELVWGGGAAAGTATAGTGACTCTATTACTGACGCAAGAAATTTCTACGCA AAAWLYRATNDNTYLNAGCGCAGACTACAGAGACGAGTTGGTTTGGGCTGCTGCGTGGTTAT TAESLYDEFGLQNWGGACAGAGCGACCAACGACAACACCTACCTCAACACTGCTGAGTCACT GLNWDSKVSGVQVLLAGTACGATGAGTTTGGGCTACAGAACTGGGGGGGGGGCCTGAACTGG KLTNKQAYKDTVQSYVGATAGCAAGGTGTCTGGCGTGCAGGTGTTGTTGGCCAAGCTTACCA NYLINNQQKTPKGLLYATAAGCAGGCCTACAAGGACACGGTGCAGTCTTACGTCAATTACCT IDMWGTLRHAANAAFIAATTAATAACCAGCAGAAGACTCCCAAGGGCCTCCTCTACATCGAC MLEAAELGLSASSYRQATGTGGGGCACCCTTCGCCACGCTGCCAACGCCGCATTCATCATGC FAQTQIDYALGDGGRSTCGAAGCCGCCGAGCTGGGCTTGTCCGCCTCCTCTTATAGACAGTT FVCGFGSNPPTRPHHRCGCGCAAACGCAAATCGACTACGCCCTGGGCGATGGTGGCCGCTCC SSSCPPAPATCDWNTFTTTGTGTGCGGGTTCGGGAGTAATCCTCCTACGAGACCGCACCACA NSPDPNYHVLSGALVGGATCCTCGTCGTGCCCGCCAGCTCCCGCTACTTGCGACTGGAATAC GPDQNDNYVDDRSDYVATTCAACTCACCTGACCCAAACTACCACGTCCTCTCTGGGGCCCTA HNEVATDYNAGFQSALGTGGGCGGACCTGATCAGAATGACAACTACGTCGATGACCGTTCAG AALVALGY* (SEQACTATGTTCACAACGAAGTCGCCACTGATTACAACGCGGGTTTCCA ID NO: 69)GTCCGCGTTAGCTGCTTTGGTGGCCCTTGGTTACTGA (SEQ ID NO: 141) 2042HvAleSP:P54583 MAHARVLLLALAVLATATGGCCCACGCCCGCGTCCTCCTCCTGGCGCTCGCCGTCCTGGCCA AAVAVASSSSFADSNPCCGCCGCCGTCGCCGTCGCCTCCTCCTCCTCCTTCGCCGACTCCAA IRPVTDRAASTAGGGYCCCGATCCGCCCGGTGACCGACCGCGCCGCCTCCACCGCTGGAGGA WHTSGREILDANNVPVGGATACTGGCACACTTCCGGCAGGGAGATCCTCGACGCAAATAACG RIAGINWFGFETCNYVTTCCAGTCAGAATCGCCGGGATTAATTGGTTTGGCTTCGAAACGTG VHGLWSRDYRSMLDQITAACTACGTGGTTCACGGCCTGTGGTCTCGGGATTACAGATCAATG KSLGYNTIRLPYSDDICTCGACCAGATCAAATCCTTGGGGTATAATACAATTAGGCTGCCCT LKPGTMPNSINFYQMNACAGCGATGACATTCTTAAGCCTGGAACCATGCCGAACTCGATTAA QDLQGLTSLQVMDKIVTTTCTACCAAATGAACCAGGATCTGCAGGGATTGACTTCTCTGCAG AYAGQIGLRIILDRHRGTTATGGACAAGATCGTGGCGTACGCCGGCCAAATCGGGCTCAGAA PDCSGQSALWYTSSVSTTATTTTGGATCGGCACAGGCCAGACTGCTCAGGTCAGTCGGCCCT EATWISDLQALAQRYKGTGGTACACAAGCTCCGTGTCAGAGGCAACATGGATTTCAGATCTT GNPTVVGFDLHNEPHDCAAGCCCTCGCACAACGCTATAAAGGCAACCCCACGGTTGTGGGAT PACWGCGDPSIDWRLATCGACCTTCACAACGAACCTCACGATCCGGCCTGTTGGGGCTGCGG AERAGNAVLSVNPNLLGGACCCTTCGATCGACTGGAGACTGGCAGCGGAGAGGGCTGGTAAC IFVEGVQSYNGDSYWWGCCGTTCTCAGCGTCAATCCCAACTTGCTGATCTTTGTGGAGGGAG GGNLQGAGQYPVVLNVTTCAGTCCTACAACGGCGATTCTTACTGGTGGGGCGGAAATCTCCA PNRLVYSAHDYATSVYAGGCGCAGGGCAGTATCCTGTCGTGCTTAACGTTCCGAATCGCCTG PQTWFSDPTFPNNMPGGTCTACTCAGCACACGACTACGCGACTAGCGTGTACCCACAGACGT IWNKNWGYLFNQNIAPGGTTCTCCGATCCCACATTTCCTAACAATATGCCGGGAATCTGGAA VWLGEFGTTLQSTTDQCAAGAATTGGGGTTACTTGTTTAACCAAAACATTGCTCCAGTTTGG TWLKTLVQYLRPTAQYTTGGGTGAATTTGGCACCACTCTTCAGTCGACGACAGACCAAACCT GADSFQWTFWSWNPDSGGCTGAAAACCCTCGTCCAGTATTTGCGGCCAACTGCTCAGTACGG GDTGGILKDDWQTVDTAGCAGATTCTTTTCAATGGACGTTCTGGTCTTGGAATCCTGACTCC VKDGYLAPIKSSIFDPGGGGATACAGGCGGTATCCTGAAAGACGATTGGCAGACCGTGGACA VGASASPSSQPSPSVSCTGTTAAGGACGGGTACTTGGCGCCGATTAAAAGCTCGATCTTTGA PSPSPSPSASRTPTPTCCCAGTCGGCGCTAGCGCTTCCCCATCTTCACAACCTTCGCCGAGC PTPTASPTPTLTPTATGTCAGCCCCAGCCCAAGCCCAAGCCCGTCTGCCAGCAGAACCCCCA PTPTASPTPSPTAASGCTCCCACACCTACCCCCACGGCCTCACCAACTCCGACGCTCACTCC ARCTASYQVNSDWGNGTACGGCGACGCCAACACCAACTGCTTCACCCACTCCTAGCCCCACC FTVTVAVTNSGSVATKGCAGCGAGCGGGGCTAGGTGCACCGCTTCTTACCAGGTCAACTCTG TWTVSWTFGGNQTITNACTGGGGTAATGGCTTCACCGTGACTGTGGCGGTCACTAACTCAGG SWNAAVTQNGQSVTARAAGCGTCGCGACGAAAACCTGGACTGTGTCCTGGACGTTCGGGGGC NMSYNNVIQPGQNTTFAACCAAACAATCACCAACAGCTGGAACGCTGCAGTTACGCAGAATG GFQASYTGSNAAPTVAGGCAAAGCGTCACGGCGCGCAATATGAGCTACAACAACGTGATTCA CAAS* (SEQ IDACCAGGCCAGAATACCACATTCGGTTTTCAAGCAAGCTATACCGGG NO: 70)TCAAACGCTGCCCCAACTGTCGCTTGTGCTGCCTCATGA (SEQ ID NO: 142) 2043 NtEGmMAYDYKQVLRDSLLFY ATGGCTTACGACTACAAGCAGGTGTTGCGGGACTCGCTACTATTCTEAQRSGRLPADQKVTW ATGAGGCCCAGAGATCCGGCCGGCTCCCAGCCGACCAGAAGGTCACRKDSALNDQGDQGQDL GTGGAGGAAGGATAGCGCGCTGAATGACCAGGGTGACCAGGGACAATGGYFDAGDFVKFGFP GACTTGACCGGCGGCTACTTTGACGCTGGGGACTTCGTCAAGTTCGMAYTATVLAWGLIDFE GGTTCCCCATGGCTTATACCGCAACCGTGCTGGCATGGGGCCTCATAGYSSAGALDDGRKAV AGATTTTGAGGCCGGCTACAGCAGTGCCGGGGCCTTGGATGATGGAKWATDYFIKAHTSQNE CGGAAGGCTGTCAAATGGGCCACCGACTATTTCATAAAGGCCCACAFYGQVGQGDADHAFWG CAAGTCAAAATGAGTTCTATGGTCAGGTCGGCCAGGGTGACGCCGARPEDMTMARPAYKIDT TCACGCTTTCTGGGGAAGACCAGAGGATATGACGATGGCGCGCCCGSRPGSDLAGETAAALA GCGTACAAGATAGACACCTCAAGGCCTGGCTCTGATCTGGCAGGCGAASIVFRNVDGTYSNN AGACAGCGGCTGCTCTTGCCGCTGCTTCAATCGTGTTCCGGAACGTLLTHARQLFDFANNYR CGATGGCACTTACTCAAATAACCTGTTAACACACGCTCGCCAGCTAGKYSDSITDARNFYAS TTCGACTTCGCGAACAACTACCGGGGAAAGTATAGTGACTCTATTAADYRDELVWAAAWLYR CTGACGCAAGAAATTTCTACGCAAGCGCAGACTACAGAGACGAGTTATNDNTYLNTAESLYD GGTTTGGGCTGCTGCGTGGTTATACAGAGCGACCAACGACAACACCEFGLQNWGGGLNWDSK TACCTCAACACTGCTGAGTCACTGTACGATGAGTTTGGGCTACAGAVSGVQVLLAKLTNKQA ACTGGGGGGGGGGCCTGAACTGGGATAGCAAGGTGTCTGGCGTGCAYKDTVQSYVNYLINNQ GGTGTTGTTGGCCAAGCTTACCAATAAGCAGGCCTACAAGGACACGQKTPKGLLYIDMWGTL GTGCAGTCTTACGTCAATTACCTAATTAATAACCAGCAGAAGACTCRHAANAAFIMLEAAEL CCAAGGGCCTCCTCTACATCGACATGTGGGGCACCCTTCGCCACGCGLSASSYRQFAQTQID TGCCAACGCCGCATTCATCATGCTCGAAGCCGCCGAGCTGGGCTTGYALGDGGRSFVCGFGS TCCGCCTCCTCTTATAGACAGTTCGCGCAAACGCAAATCGACTACGNPPTRPHHRSSSCPPA CCCTGGGCGATGGTGGCCGCTCCTTTGTGTGCGGGTTCGGGAGTAAPATCDWNTFNSPDPNY TCCTCCTACGAGACCGCACCACAGATCCTCGTCGTGCCCGCCAGCTHVLSGALVGGPDQNDN CCCGCTACTTGCGACTGGAATACATTCAACTCACCTGACCCAAACTYVDDRSDYVHNEVATD ACCACGTCCTCTCTGGGGCCCTAGTGGGCGGACCTGATCAGAATGAYNAGFQSALAALVALG CAACTACGTCGATGACCGTTCAGACTATGTTCACAACGAAGTCGCC Y* (SEQID NO: ACTGATTACAACGCGGGTTTCCAGTCCGCGTTAGCTGCTTTGGTGG 71) CCCTTGGTTACTGA(SEQ ID NO: 143) 2044 PR1a:NtEGm MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT TLLLFLVISHSCRAAYCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCGC DYKQVLRDSLLFYEAQTTACGACTACAAGCAGGTGTTGCGGGACTCGCTACTATTCTATGAG RSGRLPADQKVTWRKDGCCCAGAGATCCGGCCGGCTCCCAGCCGACCAGAAGGTCACGTGGA SALNDQGDQGQDLTGGGGAAGGATAGCGCGCTGAATGACCAGGGTGACCAGGGACAAGACTT YFDAGDFVKFGFPMAYGACCGGCGGCTACTTTGACGCTGGGGACTTCGTCAAGTTCGGGTTC TATVLAWGLIDFEAGYCCCATGGCTTATACCGCAACCGTGCTGGCATGGGGCCTCATAGATT SSAGALDDGRKAVKWATTGAGGCCGGCTACAGCAGTGCCGGGGCCTTGGATGATGGACGGAA TDYFIKAHTSQNEFYGGGCTGTCAAATGGGCCACCGACTATTTCATAAAGGCCCACACAAGT QVGQGDADHAFWGRPECAAAATGAGTTCTATGGTCAGGTCGGCCAGGGTGACGCCGATCACG DMTMARPAYKIDTSRPCTTTCTGGGGAAGACCAGAGGATATGACGATGGCGCGCCCGGCGTA GSDLAGETAAALAAASCAAGATAGACACCTCAAGGCCTGGCTCTGATCTGGCAGGCGAGACA IVFRNVDGTYSNNLLTGCGGCTGCTCTTGCCGCTGCTTCAATCGTGTTCCGGAACGTCGATG HARQLFDFANNYRGKYGCACTTACTCAAATAACCTGTTAACACACGCTCGCCAGCTATTCGA SDSITDARNFYASADYCTTCGCGAACAACTACCGGGGAAAGTATAGTGACTCTATTACTGAC RDELVWAAAWLYRATNGCAAGAAATTTCTACGCAAGCGCAGACTACAGAGACGAGTTGGTTT DNTYLNTAESLYDEFGGGGCTGCTGCGTGGTTATACAGAGCGACCAACGACAACACCTACCT LQNWGGGLNWDSKVSGCAACACTGCTGAGTCACTGTACGATGAGTTTGGGCTACAGAACTGG VQVLLAKLTNKQAYKDGGGGGGGGCCTGAACTGGGATAGCAAGGTGTCTGGCGTGCAGGTGT TVQSYVNYLINNQQKTTGTTGGCCAAGCTTACCAATAAGCAGGCCTACAAGGACACGGTGCA PKGLLYIDMWGTLRHAGTCTTACGTCAATTACCTAATTAATAACCAGCAGAAGACTCCCAAG ANAAFIMLEAAELGLSGGCCTCCTCTACATCGACATGTGGGGCACCCTTCGCCACGCTGCCA ASSYRQFAQTQIDYALACGCCGCATTCATCATGCTCGAAGCCGCCGAGCTGGGCTTGTCCGC GDGGRSFVCGFGSNPPCTCCTCTTATAGACAGTTCGCGCAAACGCAAATCGACTACGCCCTG TRPHHRSSSCPPAPATGGCGATGGTGGCCGCTCCTTTGTGTGCGGGTTCGGGAGTAATCCTC CDWNTFNSPDPNYHVLCTACGAGACCGCACCACAGATCCTCGTCGTGCCCGCCAGCTCCCGC SGALVGGPDQNDNYVDTACTTGCGACTGGAATACATTCAACTCACCTGACCCAAACTACCAC DRSDYVHNEVATDYNAGTCCTCTCTGGGGCCCTAGTGGGCGGACCTGATCAGAATGACAACT GFQSALAALVALGY*ACGTCGATGACCGTTCAGACTATGTTCACAACGAAGTCGCCACTGA (SEQ ID NO: 72)TTACAACGCGGGTTTCCAGTCCGCGTTAGCTGCTTTGGTGGCCCTT GGTTACTGA (SEQ ID NO:144) 2045 PR1a:NtEGm:SEKDEL MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT TLLLFLVISHSCRAAYCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCGC DYKQVLRDSLLFYEAQTTACGACTACAAGGAGGTGTTGCGGGACTCGCTACTATTCTATGAG RSGRLPADQKVTWRKDGCCCAGAGATCCGGCCGGCTCCCAGCCGACCAGAAGGTCACGTGGA SALNDQGDQGQDLTGGGGAAGGATAGCGCGCTGAATGACCAGGGTGACCAGGGACAAGACTT YFDAGDFVKFGFPMAYGACCGGCGGCTACTTTGACGCTGGGGACTTCGTCAAGTTCGGGTTC TATVLAWGLIDFEAGYCCCATGGCTTATACCGCAACCGTGCTGGCATGGGGCCTCATAGATT SSAGALDDGRKAVKWATTGAGGCCGGCTACAGCAGTGCCGGGGCCTTGGATGATGGACGGAA TDYFIKAHTSQNEFYGGGCTGTCAAATGGGCCACCGACTATTTCATAAAGGCCCACACAAGT QVGQGDADHAFWGRPECAAAATGAGTTCTATGGTCAGGTCGGCCAGGGTGACGCCGATCACG DMTMARPAYKIDTSRPCTTTCTGGGGAAGACCAGAGGATATGACGATGGCGCGCCCGGCGTA GSDLAGETAAALAAASCAAGATAGACACCTCAAGGCCTGGCTCTGATCTGGCAGGCGAGACA IVFRNVDGTYSNNLLTGCGGCTGCTCTTGCCGCTGCTTCAATCGTGTTCCGGAACGTCGATG HARQLFDFANNYRGKYGCACTTACTCAAATAACCTGTTAACACACGCTCGCCAGCTATTCGA SDSITDARNFYASADYCTTCGCGAACAACTACCGGGGAAAGTATAGTGACTCTATTACTGAC RDELVWAAAWLYRATNGCAAGAAATTTCTACGCAAGCGCAGACTACAGAGACGAGTTGGTTT DNTYLNTAESLYDEFGGGGCTGCTGCGTGGTTATACAGAGCGACCAACGACAACACCTACCT LQNWGGGLNWDSKVSGCAACACTGCTGAGTCACTGTACGATGAGTTTGGGCTACAGAACTGG VQVLLAKLTNKQAYKDGGGGGGGGCCTGAACTGGGATAGCAAGGTGTCTGGCGTGCAGGTGT TVQSYVNYLINNQQKTTGTTGGCCAAGCTTACCAATAAGCAGGCCTACAAGGACACGGTGCA PKGLLYIDMWGTLRHAGTCTTACGTCAATTACCTAATTAATAACCAGCAGAAGACTCCCAAG ANAAFIMLEAAELGLSGGCCTCCTCTACATCGACATGTGGGGCACCCTTCGCCACGCTGCCA ASSYRQFAQTQIDYALACGCCGCATTCATCATGCTCGAAGCCGCCGAGCTGGGCTTGTCCGC GDGGRSFVCGFGSNPPCTCCTCTTATAGACAGTTCGCGCAAACGCAAATCGACTACGCCCTG TRPHHRSSSCPPAPATGGCGATGGTGGCCGCTCCTTTGTGTGCGGGTTCGGGAGTAATCCTC CDWNTFNSPDPNYHVLCTACGAGACCGCACCACAGATCCTCGTCGTGCCCGCCAGCTCCCGC SGALVGGPDQNDNYVDTACTTGCGACTGGAATACATTCAACTCACCTGACCCAAACTACCAC DRSDYVHNEVATDYNAGTCCTCTCTGGGGCCCTAGTGGGCGGACCTGATCAGAATGACAACT GFQSALAALVALGYSEACGTCGATGACCGTTCAGACTATGTTCACAACGAAGTCGCCACTGA KDEL* (SEQ IDTTACAACGCGGGTTTCCAGTCCGCGTTAGCTGCTTTGGTGGCCCTT NO: 73)GGTTACAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 145) 2046 BAASS:NtEGm:SEKDELMANKHLSLSLFLVLLG ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTGLSASLASGQVAYDYKQ GCCTGTCGGCCAGCTTGGCCTCCGGGCAAGTCGCTTACGACTACAAVLRDSLLFYEAQRSGR GCAGGTGTTGCGGGACTCGCTACTATTCTATGAGGCCCAGAGATCCLPADQKVTWRKDSALN GGCCGGCTCCCAGCCGACCAGAAGGTCACGTGGAGGAAGGATAGCGDQGDQGQDLTGGYFDA CGCTGAATGACCAGGGTGACCAGGGACAAGACTTGACCGGCGGCTAGDFVKFGFPMAYTATV CTTTGACGCTGGGGACTTCGTCAAGTTCGGGTTCCCCATGGCTTATLAWGLIDFEAGYSSAG ACCGCAACCGTGCTGGCATGGGGCCTCATAGATTTTGAGGCCGGCTALDDGRKAVKWATDYF ACAGCAGTGCCGGGGCCTTGGATGATGGACGGAAGGCTGTCAAATGIKAHTSQNEFYGQVGQ GGCCACCGACTATTTCATAAAGGCCCACACAAGTCAAAATGAGTTCGDADHAFWGRPEDMTM TATGGTCAGGTCGGCCAGGGTGACGCCGATCACGCTTTCTGGGGAAARPAYKIDTSRPGSDL GACCAGAGGATATGACGATGGCGCGCCCGGCGTACAAGATAGACACAGETAAALAAASIVFR CTCAAGGCCTGGCTCTGATCTGGCAGGCGAGACAGCGGCTGCTCTTNVDGTYSNNLLTHARQ GCCGCTGCTTCAATCGTGTTCCGGAACGTCGATGGCACTTACTCAALFDFANNYRGKYSDSI ATAACCTGTTAACACACGCTCGCCAGCTATTCGACTTCGCGAACAATDARNFYASADYRDEL CTACCGGGGAAAGTATAGTGACTCTATTACTGACGCAAGAAATTTCVWAAAWLYRATNDNTY TACGCAAGCGCAGACTACAGAGACGAGTTGGTTTGGGCTGCTGCGTLNTAESLYDEFGLQNW GGTTATACAGAGCGACCAACGACAACACCTACCTCAACACTGCTGAGGGLNWDSKVSGVQVL GTCACTGTACGATGAGTTTGGGCTACAGAACTGGGGGGGGGGCCTGLAKLTNKQAYKDTVQS AACTGGGATAGCAAGGTGTCTGGCGTGCAGGTGTTGTTGGCCAAGCYVNYLINNQQKTPKGL TTACCAATAAGCAGGCCTACAAGGACACGGTGCAGTCTTACGTCAALYIDMWGTLRHAANAA TTACCTAATTAATAACCAGCAGAAGACTCCCAAGGGCCTCCTCTACFIMLEAAELGLSASSY ATCGACATGTGGGGCACCCTTCGCCACGCTGCCAACGCCGCATTCARQFAQTQIDYALGDGG TCATGCTCGAAGCCGCCGAGCTGGGCTTGTCCGCCTCCTCTTATAGRSFVCGFGSNPPTRPH ACAGTTCGCGCAAACGCAAATCGACTACGCCCTGGGCGATGGTGGCHRSSSCPPAPATCDWN CGCTCCTTTGTGTGCGGGTTCGGGAGTAATCCTCCTACGAGACCGCTFNSPDPNYHVLSGAL ACCACAGATCCTCGTCGTGCCCGCCAGCTCCCGCTACTTGCGACTGVGGPDQNDNYVDDRSD GAATACATTCAACTCACCTGACCCAAACTACCACGTCCTCTCTGGGYVHNEVATDYNAGFQS GCCCTAGTGGGCGGACCTGATCAGAATGACAACTACGTCGATGACCALAALVALGYSEKDEL GTTCAGACTATGTTCACAACGAAGTCGCCACTGATTACAACGCGGG * (SEQID NO: TTTCCAGTCCGCGTTAGCTGCTTTGGTGGCCCTTGGTTACAGCGAG 74)AAGGACGAGCTGTGA (SEQ ID NO: 146) 2047 HvAleSP:P54583: MAHARVLLLALAVLATATGGCCCACGCCCGCGTCCTCCTCCTGGCGCTCGCCGTCCTGGCCA SEKDEL AAVAVASSSSFADSNPCCGCCGCCGTCGCCGTCGCCTCCTCCTCCTCCTTCGCCGACTCCAA IRPVTDRAASTAGGGYCCCGATCCGCCCGGTGACCGACCGCGCCGCCTCCACCGCTGGAGGA WHTSGREILDANNVPVGGATACTGGCACACTTCCGGCAGGGAGATCCTCGACGCAAATAACG RIAGINWFGFETCNYVTTCCAGTCAGAATCGCCGGGATTAATTGGTTTGGCTTCGAAACGTG VHGLWSRDYRSMLDQITAACTACGTGGTTCACGGCCTGTGGTCTCGGGATTACAGATCAATG KSLGYNTIRLPYSDDICTCGACCAGATCAAATCCTTGGGGTATAATACAATTAGGCTGCCCT LKPGTMPNSINFYQMNACAGCGATGACATTCTTAAGCCTGGAACCATGCCGAACTCGATTAA QDLQGLTSLQVMDKIVTTTCTACCAAATGAACCAGGATCTGCAGGGATTGACTTCTCTGCAG AYAGQIGLRIILDRHRGTTATGGACAAGATCGTGGCGTACGCCGGCCAAATCGGGCTCAGAA PDCSGQSALWYTSSVSTTATTTTGGATCGGCACAGGCCAGACTGCTCAGGTCAGTCGGCCCT EATWISDLQALAQRYKGTGGTACACAAGCTCCGTGTCAGAGGCAACATGGATTTCAGATCTT GNPTVVGFDLHNEPHDCAAGCCCTCGCACAACGCTATAAAGGCAACCCCACGGTTGTGGGAT PACWGCGDPSIDWRLATCGACCTTCACAACGAACCTCACGATCCGGCCTGTTGGGGCTGCGG AERAGNAVLSVNPNLLGGACCCTTCGATCGACTGGAGACTGGCAGCGGAGAGGGCTGGTAAC IFVEGVQSYNGDSYWWGCCGTTCTCAGCGTCAATCCCAACTTGCTGATCTTTGTGGAGGGAG GGNLQGAGQYPVVLNVTTCAGTCCTACAACGGCGATTCTTACTGGTGGGGCGGAAATCTCCA PNRLVYSAHDYATSVYAGGCGCAGGGCAGTATCCTGTCGTGCTTAACGTTCCGAATCGCCTG PQTWFSDPTFPNNMPGGTCTACTCAGCACACGACTACGCGACTAGCGTGTACCCACAGACGT IWNKNWGYLFNQNIAPGGTTCTCCGATCCCACATTTCCTAACAATATGCCGGGAATCTGGAA VWLGEFGTTLQSTTDQCAAGAATTGGGGTTACTTGTTTAACCAAAACATTGCTCCAGTTTGG TWLKTLVQYLRPTAQYTTGGGTGAATTTGGCACCACTCTTCAGTCGACGACAGACCAAACCT GADSFQWTFWSWNPDSGGCTGAAAACCCTCGTCCAGTATTTGCGGCCAACTGCTCAGTACGG GDTGGILKDDWQTVDTAGCAGATTCTTTTCAATGGACGTTCTGGTCTTGGAATCCTGACTCC VKDGYLAPIKSSIFDPGGGGATACAGGCGGTATCCTGAAAGACGATTGGCAGACCGTGGACA VGASASPSSQPSPSVSCTGTTAAGGACGGGTACTTGGCGCCGATTAAAAGCTCGATCTTTGA PSPSPSPSASRTPTPTCCCAGTCGGCGCTAGCGCTTCCCCATCTTCACAACCTTCGCCGAGC PTPTASPTPTLTPTATGTCAGCCCCAGCCCAAGCCCAAGCCCGTCTGCCAGCAGAACCCCCA PTPTASPTPSPTAASGCTCCCACACCTACCCCCACGGCCTCACCAACTCCGACGCTCACTCC ARCTASYQVNSDWGNGTACGGCGACGCCAACACCAACTGCTTCACCCACTCCTAGCCCCACC FTVTVAVTNSGSVATKGCAGCGAGCGGGGCTAGGTGCACCGCTTCTTACCAGGTCAACTCTG TWTVSWTFGGNQTITNACTGGGGTAATGGCTTCACCGTGACTGTGGCGGTCACTAACTCAGG SWNAAVTQNGQSVTARAAGCGTCGCGACGAAAACCTGGACTGTGTCCTGGACGTTCGGGGGC NMSYNNVIQPGQNTTFAACCAAACAATCACCAACAGCTGGAACGCTGCAGTTACGCAGAATG GFQASYTGSNAAPTVAGGCAAAGCGTCACGGCGCGCAATATGAGCTACAACAACGTGATTCA CAASSEKDEL*ACCAGGCCAGAATACCACATTCGGTTTTCAAGCAAGCTATACCGGG (SEQ ID NO: 75)TCAAACGCTGCCCCAACTGTCGCTTGTGCTGCCTCAAGCGAGAAGG ACGAGCTGTGA (SEQ ID NO:147) 2048 HvAleSP:NtEGm MAHARVLLLALAVLATATGGCCCACGCCCGCGTCCTCCTCCTGGCGCTCGCCGTCCTGGCCA AAVAVASSSSFADSNPCCGCCGCCGTCGCCGTCGCCTCCTCCTCCTCCTTCGCCGACTCCAA IRPVTDRAASTAYDYKCCCGATCCGCCCGGTGACCGACCGCGCCGCCTCCACCGCTTACGAC QVLRDSLLFYEAQRSGTACAAGCAGGTGTTGCGGGACTCGCTACTATTCTATGAGGCCCAGA RLPADQKVTWRKDSALGATCCGGCCGGCTCCCAGCCGACCAGAAGGTCACGTGGAGGAAGGA NDQGDQGQDLTGGYFDTAGCGCGCTGAATGACCAGGGTGACCAGGGACAAGACTTGACCGGC AGDFVKFGFPMAYTATGGCTACTTTGACGCTGGGGACTTCGTCAAGTTCGGGTTCCCCATGG VLAWGLIDFEAGYSSACTTATACCGCAACCGTGCTGGCATGGGGCCTCATAGATTTTGAGGC GALDDGRKAVKWATDYCGGCTACAGCAGTGCCGGGGCCTTGGATGATGGACGGAAGGCTGTC FIKAHTSQNEFYGQVGAAATGGGCCACCGACTATTTCATAAAGGCCCACACAAGTCAAAATG QGDADHAFWGRPEDMTAGTTCTATGGTCAGGTCGGCCAGGGTGACGCCGATCACGCTTTCTG MARPAYKIDTSRPGSDGGGAAGACCAGAGGATATGACGATGGCGCGCCCGGCGTACAAGATA LAGETAAALAAASIVFGACACCTCAAGGCCTGGCTCTGATCTGGCAGGCGAGACAGCGGCTG RNVDGTYSNNLLTHARCTCTTGCCGCTGCTTCAATCGTGTTCCGGAACGTCGATGGCACTTA QLFDFANNYRGKYSDSCTCAAATAACCTGTTAACACACGCTCGCCAGCTATTCGACTTCGCG ITDARNFYASADYRDEAACAACTACCGGGGAAAGTATAGTGACTCTATTACTGACGCAAGAA LVWAAAWLYRATNDNTATTTCTACGCAAGCGCAGACTACAGAGACGAGTTGGTTTGGGCTGC YLNTAESLYDEFGLQNTGCGTGGTTATACAGAGCGACCAACGACAACACCTACCTCAACACT WGGGLNWDSKVSGVQVGCTGAGTCACTGTACGATGAGTTTGGGCTACAGAACTGGGGGGGGG LLAKLTNKQAYKDTVQGCCTGAACTGGGATAGCAAGGTGTCTGGCGTGCAGGTGTTGTTGGC SYVNYLINNQQKTPKGCAAGCTTACCAATAAGCAGGCCTACAAGGACACGGTGCAGTCTTAC LLYIDMWGTLRHAANAGTCAATTACCTAATTAATAACCAGCAGAAGACTCCCAAGGGCCTCC AFIMLEAAELGLSASSTCTACATCGACATGTGGGGCACCCTTCGCCACGCTGCCAACGCCGC YRQFAQTQIDYALGDGATTCATCATGCTCGAAGCCGCCGAGCTGGGCTTGTCCGCCTCCTCT GRSFVCGFGSNPPTRPTATAGACAGTTCGCGCAAACGCAAATCGACTACGCCCTGGGCGATG HHRSSSCPPAPATCDWGTGGCCGCTCCTTTGTGTGCGGGTTCGGGAGTAATCCTCCTACGAG NTFNSPDPNYHVLSGAACCGCACCACAGATCCTCGTCGTGCCCGCCAGCTCCCGCTACTTGC LVGGPDQNDNYVDDRSGACTGGAATACATTCAACTCACCTGACCCAAACTACCACGTCCTCT DYVHNEVATDYNAGFQCTGGGGCCCTAGTGGGCGGACCTGATCAGAATGACAACTACGTCGA SALAALVALGY*TGACCGTTCAGACTATGTTCACAACGAAGTCGCCACTGATTACAAC (SEQ ID NO: 76)GCGGGTTTCCAGTCCGCGTTAGCTGCTTTGGTGGCCCTTGGTTACT GA (SEQ ID NO: 148) 2049HvAleSP:NtEGm: MAHARVLLLALAVLATATGGCCCACGCCCGCGTCCTCCTCCTGGCGCTCGCCGTCCTGGCCA SEKDEL AAVAVASSSSFADSNPCCGCCGCCGTCGCCGTCGCCTCCTCCTCCTCCTTCGCCGACTCCAA IRPVTDRAASTAYDYKCCCGATCCGCCCGGTGACCGACCGCGCCGCCTCCACCGCTTACGAC QVLRDSLLFYEAQRSGTACAAGCAGGTGTTGCGGGACTCGCTACTATTCTATGAGGCCCAGA RLPADQKVTWRKDSALGATCCGGCCGGCTCCCAGCCGACCAGAAGGTCACGTGGAGGAAGGA NDQGDQGQDLTGGYFDTAGCGCGCTGAATGACCAGGGTGACCAGGGACAAGACTTGACCGGC AGDFVKFGFPMAYTATGGCTACTTTGACGCTGGGGACTTCGTCAAGTTCGGGTTCCCCATGG VLAWGLIDFEAGYSSACTTATACCGCAACCGTGCTGGCATGGGGCCTCATAGATTTTGAGGC GALDDGRKAVKWATDYCGGCTACAGGAGTGCCGGGGCCTTGGATGATGGACGGAAGGCTGTC FIKAHTSQNEFYGQVGAAATGGGCCACCGACTATTTCATAAAGGCCCACACAAGTCAAAATG QGDADHAFWGRPEDMTAGTTCTATGGTCAGGTCGGCCAGGGTGACGCCGATCACGCTTTCTG MARPAYKIDTSRPGSDGGGAAGACCAGAGGATATGACGATGGCGCGCCCGGCGTACAAGATA LAGETAAALAAASIVFGACACCTCAAGGCCTGGCTCTGATCTGGCAGGCGAGACAGCGGCTG RNVDGTYSNNLLTHARCTCTTGCCGCTGCTTCAATCGTGTTCCGGAACGTCGATGGCACTTA QLFDFANNYRGKYSDSCTCAAATAACCTGTTAACACACGCTCGCCAGCTATTCGACTTCGCG ITDARNFYASADYRDEAACAACTACCGGGGAAAGTATAGTGACTCTATTACTGACGCAAGAA LVWAAAWLYRATNDNTATTTCTACGCAAGCGCAGACTACAGAGACGAGTTGGTTTGGGCTGC YLNTAESLYDEFGLQNTGCGTGGTTATACAGAGCGACCAACGACAACACCTACCTCAACACT WGGGLNWDSKVSGVQVGCTGAGTCACTGTACGATGAGTTTGGGCTACAGAACTGGGGGGGGG LLAKLTNKQAYKDTVQGCCTGAACTGGGATAGCAAGGTGTCTGGCGTGCAGGTGTTGTTGGC SYVNYLINNQQKTPKGCAAGCTTACCAATAAGCAGGCCTACAAGGACACGGTGCAGTCTTAC LLYIDMWGTLRHAANAGTCAATTACCTAATTAATAACCAGCAGAAGACTCCCAAGGGCCTCC AFIMLEAAELGLSASSTCTACATCGACATGTGGGGCACCCTTCGCCACGCTGCCAACGCCGC YRQFAQTQIDYALGDGATTCATCATGCTCGAAGCCGCCGAGCTGGGCTTGTCCGCCTCCTCT GRSFVCGFGSNPPTRPTATAGACAGTTCGCGCAAACGCAAATCGACTACGCCCTGGGCGATG HHRSSSCPPAPATCDWGTGGCCGCTCCTTTGTGTGCGGGTTCGGGAGTAATCCTCCTACGAG NTFNSPDPNYHVLSGAACCGCACCACAGATCCTCGTCGTGCCCGCCAGCTCCCGCTACTTGC LVGGPDQNDNYVDDRSGACTGGAATACATTCAACTCACCTGACCCAAACTACCACGTCCTCT DYVHNEVATDYNAGFQCTGGGGCCCTAGTGGGCGGACCTGATCAGAATGACAACTACGTCGA SALAALVALGYSEKDETGACCGTTCAGACTATGTTCACAACGAAGTCGCCACTGATTACAAC L* (SEQ ID NO:GCGGGTTTCCAGTCCGCGTTAGCTGCTTTGGTGGCCCTTGGTTACA 77) GCGAGAAGGACGAGCTGTGA(SEQ ID NO: 149) 2050 P26222 MNDSPFYVNPNMSSAEATGAACGATAGTCCATTCTACGTGAACCCGAATATGTCATCAGCTG WVRNNPNDPRTPVIRDAGTGGGTGCGTAACAACCCCAATGACCCTCGCACACCAGTCATTAG RIASVPQGTWFAHHNPGGATCGTATTGCCTCGGTGCCCCAAGGAACGTGGTTCGCCCACCAT GQITGQVDALMSAAQAAACCCTGGCCAGATTACAGGGCAAGTTGATGCTCTGATGTCCGCCG AGKIPILVVYNAPGRDCTCAAGCCGCGGGTAAGATCCCTATTCTCGTGGTGTACAACGCACC CGNHSSGGAPSHSAYRAGGACGCGACTGCGGGAATCATAGTTCGGGTGGGGCTCCTTCCCAC SWIDEFAAGLKNRPAYAGCGCTTATCGGTCTTGGATCGACGAGTTTGCTGCTGGCCTCAAGA IIVEPDLISLMSSCMQACCGTCCCGCTTACATCATTGTGGAGCCTGACCTGATAAGCCTTAT HVQQEVLETMAYAGKAGTCGTCGTGTATGCAGCACGTTCAACAGGAGGTGCTCGAGACTATG LKAGSSQARIYFDAGHGCCTACGCAGGGAAGGCCTTGAAGGCCGGCTCATCCCAGGCCCGTA SAWHSPAQMASWLQQATCTATTTCGACGCGGGGCATTCGGCGTGGCATTCACCAGCGCAGAT DISNSAHGIATNTSNYGGCTTCTTGGCTCCAGCAGGCTGATATCTCAAACTCTGCACATGGT RWTADEVAYAKAVLSAATCGCCACGAATACTTCTAACTACCGTTGGACCGCTGATGAAGTCG IGNPSLRAVIDTSRNGCGTACGCCAAGGCCGTGCTGTCCGCCATAGGAAATCCCTCCCTCAG NGPAGNEWCDPSGRAIAGCCGTCATAGATACGTCCCGCAACGGAAATGGCCCTGCTGGAAAT GTPSTTNTGDPMIDAFGAGTGGTGCGACCCAAGCGGACGCGCTATCGGAACCCCGAGTACCA LWIKLPGEADGCIAGACAAATACTGGCGACCCAATGATCGATGCTTTCCTCTGGATTAAGCT GQFVPQAAYEMAIAAGTCCGGGAGAAGCAGACGGTTGCATCGCCGGAGCTGGCCAATTCGTT GTNPNPNPNPTPTPTPCCACAAGCAGCATACGAGATGGCTATTGCGGCGGGTGGTACGAATC TPTPPPGSSGACTATYCTAATCCCAACCCCAACCCTACGCCAACGCCCACACCGACTCCCAC TIANEWNDGFQATVTVTCCACCTCCGGGGAGCAGCGGCGCCTGCACAGCCACCTATACAATC TANQNITGWTVTWTFTGCAAACGAATGGAATGATGGCTTCCAAGCGACGGTGACGGTGACCG DGQTITNAWNADVSTSCGAACCAGAACATCACTGGGTGGACTGTCACTTGGACTTTCACGGA GSSVTARNVGHNGTLSTGGACAGACTATTACTAACGCCTGGAATGCTGACGTTTCGACGTCA QGASTEFGFVGSKGNSGGTTCGTCTGTGACGGCGCGCAACGTCGGGCATAATGGTACTCTCT NSVPTLTCAAS*CCCAGGGCGCCAGCACAGAGTTTGGCTTTGTCGGCTCAAAGGGAAA (SEQ ID NO: 78)TTCAAATAGCGTCCCCACTCTCACGTGCGCCGCCTCGTGA (SEQ ID NO: 150) 2051PR1a:P26222 MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT TLLLFLVISHSCRANDCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCAA SPFYVNPNMSSAEWVRCGATAGTCCATTCTACGTGAACCCGAATATGTCATCAGCTGAGTGG NNPNDPRTPVIRDRIAGTGCGTAACAACCCCAATGACCCTCGCACACCAGTCATTAGGGATC SVPQGTWFAHHNPGQIGTATTGCCTCGGTGCCCCAAGGAACGTGGTTCGCCCACCATAACCC TGQVDALMSAAQAAGKTGGCCAGATTACAGGGCAAGTTGATGCTCTGATGTCCGCCGCTCAA IPILVVYNAPGRDCGNGCCGCGGGTAAGATCCCTATTCTCGTGGTGTACAACGCACCAGGAC HSSGGAPSHSAYRSWIGCGACTGCGGGAATCATAGTTCGGGTGGGGCTCCTTCCCACAGCGC DEFAAGLKNRPAYIIVTTATCGGTCTTGGATCGACGAGTTTGCTGCTGGCCTCAAGAACCGT EPDLISLMSSCMQHVQCCCGCTTACATCATTGTGGAGCCTGACCTGATAAGCCTTATGTCGT QEVLETMAYAGKALKACGTGTATGCAGCACGTTCAACAGGAGGTGCTCGAGACTATGGCCTA GSSQARIYFDAGHSAWCGCAGGGAAGGCCTTGAAGGCCGGCTCATCCCAGGCCCGTATCTAT HSPAQMASWLQQADISTTCGACGCGGGGCATTCGGCGTGGCATTCACCAGCGCAGATGGCTT NSAHGIATNTSNYRWTCTTGGCTCCAGCAGGCTGATATCTCAAACTCTGCACATGGTATCGC ADEVAYAKAVLSAIGNCACGAATACTTCTAACTACCGTTGGACCGCTGATGAAGTCGCGTAC PSLRAVIDTSRNGNGPGCCAAGGCCGTGCTGTCCGCCATAGGAAATCCCTCCCTCAGAGCCG AGNEWCDPSGRAIGTPTCATAGATACGTCCCGCAACGGAAATGGCCCTGCTGGAAATGAGTG STTNTGDPMIDAFLWIGTGCGACCCAAGCGGACGCGCTATCGGAACCCCGAGTACCACAAAT KLPGEADGCIAGAGQFACTGGCGACCCAATGATCGATGCTTTCCTCTGGATTAAGCTTCCGG VPQAAYEMAIAAGGTNGAGAAGCAGACGGTTGCATCGCCGGAGCTGGCCAATTCGTTCCACA PNPNPNPTPTPTPTPTAGCAGCATACGAGATGGCTATTGCGGCGGGTGGTACGAATCCTAAT PPPGSSGACTATYTIACCCAACCCCAACCCTACGCCAACGCCCACACCGACTCCCACTCCAC NEWNDGFQATVTVTANCTCCGGGGAGCAGCGGCGCCTGCACAGCCACCTATACAATCGCAAA QNITGWTVTWTFTDGQCGAATGGAATGATGGCTTCCAAGCGACGGTGACGGTGACCGCGAAC TITNAWNADVSTSGSSCAGAACATCACTGGGTGGACTGTCACTTGGACTTTCACGGATGGAC VTARNVGHNGTLSQGAAGACTATTACTAACGCCTGGAATGCTGACGTTTCGACGTCAGGTTC STEFGFVGSKGNSNSVGTCTGTGACGGCGCGCAACGTCGGGCATAATGGTACTCTCTCCCAG PTLTCAAS* (SEQGGCGCCAGCACAGAGTTTGGCTTTGTCGGCTCAAAGGGAAATTCAA ID NO: 79)ATAGCGTCCCCACTCTCACGTGCGCCGCCTCGTGA (SEQ ID NO: 151) 2052PR1a:P26222:SEKDEL MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT TLLLFLVISHSCRANDCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCAA SPFYVNPNMSSAEWVRCGATAGTCCATTCTACGTGAACCCGAATATGTCATCAGCTGAGTGG NNPNDPRTPVIRDRIAGTGCGTAACAACCCCAATGACCCTCGCACACCAGTCATTAGGGATC SVPQGTWFAHHNPGQIGTATTGCCTCGGTGCCCCAAGGAACGTGGTTCGCCCACCATAACCC TGQVDALMSAAQAAGKTGGCCAGATTACAGGGCAAGTTGATGCTCTGATGTCCGCCGCTCAA IPILVVYNAPGRDCGNGCCGCGGGTAAGATCCCTATTCTCGTGGTGTACAACGCACCAGGAC HSSGGAPSHSAYRSWIGCGACTGCGGGAATCATAGTTCGGGTGGGGCTCCTTCCCACAGCGC DEFAAGLKNRPAYIIVTTATCGGTCTTGGATCGACGAGTTTGCTGCTGGCCTCAAGAACCGT EPDLISLMSSCMQHVQCCCGCTTACATCATTGTGGAGCCTGACCTGATAAGCCTTATGTCGT QEVLETMAYAGKALKACGTGTATGCAGCACGTTCAACAGGAGGTGCTCGAGACTATGGCCTA GSSQARIYFDAGHSAWCGCAGGGAAGGCCTTGAAGGCCGGCTCATCCCAGGCCCGTATCTAT HSPAQMASWLQQADISTTCGACGCGGGGCATTCGGCGTGGCATTCACCAGCGCAGATGGCTT NSAHGIATNTSNYRWTCTTGGCTCCAGCAGGCTGATATCTCAAACTCTGCACATGGTATCGC ADEVAYAKAVLSAIGNCACGAATACTTCTAACTACCGTTGGACCGCTGATGAAGTCGCGTAC PSLRAVIDTSRNGNGPGCCAAGGCCGTGCTGTCCGCCATAGGAAATCCCTCCCTCAGAGCCG AGNEWCDPSGRAIGTPTCATAGATACGTCCCGCAACGGAAATGGCCCTGCTGGAAATGAGTG STTNTGDPMIDAFLWIGTGCGACCCAAGCGGACGCGCTATCGGAACCCCGAGTACCACAAAT KLPGEADGCIAGAGQFACTGGCGACCCAATGATCGATGCTTTCCTCTGGATTAAGCTTCCGG VPQAAYEMAIAAGGTNGAGAAGCAGACGGTTGCATCGCCGGAGCTGGCCAATTCGTTCCACA PNPNPNPTPTPTPTPTAGCAGCATACGAGATGGCTATTGCGGCGGGTGGTACGAATCCTAAT PPPGSSGACTATYTIACCCAACCCCAACCCTACGCCAACGCCCACACCGACTCCCACTCCAC NEWNDGFQATVTVTANCTCCGGGGAGCAGCGGCGCCTGCACAGCCACCTATACAATCGCAAA QNITGWTVTWTFTDGQCGAATGGAATGATGGCTTCCAAGCGACGGTGACGGTGACCGCGAAC TITNAWNADVSTSGSSCAGAACATCACTGGGTGGACTGTCACTTGGACTTTCACGGATGGAC VTARNVGHNGTLSQGAAGACTATTACTAACGCCTGGAATGCTGACGTTTCGACGTCAGGTTC STEFGFVGSKGNSNSVGTCTGTGACGGCGCGCAACGTCGGGCATAATGGTACTCTCTCCCAG PTLTCAASSEKDEL*GGCGCCAGCACAGAGTTTGGCTTTGTCGGCTCAAAGGGAAATTCAA (SEQ ID NO: 80)ATAGCGTCCCCACTCTCACGTGCGCCGCCTCGAGCGAGAAGGACGA GCTGTGA (SEQ ID NO: 152)2053 BAASS:P26222 MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQNDSPFYVGCCTGTCGGCCAGCTTGGCCTCCGGGCAAAACGATAGTCCATTCTA NPNMSSAEWVRNNPNDCGTGAACCCGAATATGTCATCAGCTGAGTGGGTGCGTAACAACCCC PRTPVIRDRIASVPQGAATGACCCTCGCACACCAGTCATTAGGGATCGTATTGCCTCGGTGC TWFAHHNPGQITGQVDCCCAAGGAACGTGGTTCGCCCACCATAACCCTGGCCAGATTACAGG ALMSAAQAAGKIPILVGCAAGTTGATGCTCTGATGTCCGCCGCTCAAGCCGCGGGTAAGATC VYNAPGRDCGNHSSGGCCTATTCTCGTGGTGTACAACGCACCAGGACGCGACTGCGGGAATC APSHSAYRSWIDEFAAATAGTTCGGGTGGGGCTCCTTCCCACAGCGCTTATCGGTCTTGGAT GLKNRPAYIIVEPDLICGACGAGTTTGCTGCTGGCCTCAAGAACCGTCCCGCTTACATCATT SLMSSCMQHVQQEVLEGTGGAGCCTGACCTGATAAGCCTTATGTCGTCGTGTATGCAGCACG TMAYAGKALKAGSSQATTCAACAGGAGGTGCTCGAGACTATGGCCTACGCAGGGAAGGCCTT RIYFDAGHSAWHSPAQGAAGGCCGGCTCATCCCAGGCCCGTATCTATTTCGACGCGGGGCAT MASWLQQADISNSAHGTCGGCGTGGCATTCACCAGCGCAGATGGCTTCTTGGCTCCAGCAGG IATNTSNYRWTADEVACTGATATCTCAAACTCTGCACATGGTATCGCCACGAATACTTCTAA YAKAVLSAIGNPSLRACTACCGTTGGACCGCTGATGAAGTCGCGTACGCCAAGGCCGTGCTG VIDTSRNGNGPAGNEWTCCGCCATAGGAAATCCCTCCCTCAGAGCCGTCATAGATACGTCCC CDPSGRAIGTPSTTNTGCAACGGAAATGGCCCTGCTGGAAATGAGTGGTGCGACCCAAGCGG GDPMIDAFLWIKLPGEACGCGCTATCGGAACCCCGAGTACCACAAATACTGGCGACCCAATG ADGCIAGAGQFVPQAAATCGATGCTTTCCTCTGGATTAAGCTTCCGGGAGAAGCAGACGGTT YEMAIAAGGTNPNPNPGCATCGCCGGAGCTGGCCAATTCGTTCCACAAGCAGCATACGAGAT NPTPTPTPTPTPPPGSGGCTATTGCGGCGGGTGGTACGAATCCTAATCCCAACCCCAACCCT SGACTATYTIANEWNDACGCCAACGCCCACACCGACTCCCACTCCACCTCCGGGGAGCAGCG GFQATVTVTANQNITGGCGCCTGCACAGCCACCTATACAATCGCAAACGAATGGAATGATGG WTVTWTFTDGQTITNACTTCCAAGCGACGGTGACGGTGACCGCGAACCAGAACATCACTGGG WNADVSTSGSSVTARNTGGACTGTCACTTGGACTTTCACGGATGGACAGACTATTACTAACG VGHNGTLSQGASTEFGCCTGGAATGCTGACGTTTCGACGTCAGGTTCGTCTGTGACGGCGCG FVGSKGNSNSVPTLTCCAACGTCGGGCATAATGGTACTCTCTCCCAGGGCGCCAGCACAGAG AAS* (SEQ IDTTTGGCTTTGTCGGCTCAAAGGGAAATTCAAATAGCGTCCCCACTC NO: 81)TCACGTGCGCCGCCTCGTGA (SEQ ID NO: 153) 2054 BAASS:P26222:SEKDELMANKHLSLSLFLVLLG ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTGLSASLASGQNDSPFYV GCCTGTCGGCCAGCTTGGCCTCCGGGCAAAACGATAGTCCATTCTANPNMSSAEWVRNNPND CGTGAACCCGAATATGTCATCAGCTGAGTGGGTGCGTAACAACCCCPRTPVIRDRIASVPQG AATGACCCTCGCACACCAGTCATTAGGGATCGTATTGCCTCGGTGCTWFAHHNPGQITGQVD CCCAAGGAACGTGGTTCGCCCACCATAACCCTGGCCAGATTACAGGALMSAAQAAGKIPILV GCAAGTTGATGCTCTGATGTCCGCCGCTCAAGCCGCGGGTAAGATCVYNAPGRDCGNHSSGG CCTATTCTCGTGGTGTACAACGCACCAGGACGCGACTGCGGGAATCAPSHSAYRSWIDEFAA ATAGTTCGGGTGGGGCTCCTTCCCACAGCGCTTATCGGTCTTGGATGLKNRPAYIIVEPDLI CGACGAGTTTGCTGCTGGCCTCAAGAACCGTCCCGCTTACATCATTSLMSSCMQHVQQEVLE GTGGAGCCTGACCTGATAAGCCTTATGTCGTCGTGTATGCAGCACGTMAYAGKALKAGSSQA TTCAACAGGAGGTGCTCGAGACTATGGCCTACGCAGGGAAGGCCTTRIYFDAGHSAWHSPAQ GAAGGCCGGCTCATCCCAGGCCCGTATCTATTTCGACGCGGGGCATMASWLQQADISNSAHG TCGGCGTGGCATTCACCAGCGCAGATGGCTTCTTGGCTCCAGCAGGIATNTSNYRWTADEVA CTGATATCTCAAACTCTGCACATGGTATCGCCACGAATACTTCTAAYAKAVLSAIGNPSLRA CTACCGTTGGACCGCTGATGAAGTCGCGTACGCCAAGGCCGTGCTGVIDTSRNGNGPAGNEW TCCGCCATAGGAAATCCCTCCCTCAGAGCCGTCATAGATACGTCCCCDPSGRAIGTPSTTNT GCAACGGAAATGGCCCTGCTGGAAATGAGTGGTGCGACCCAAGCGGGDPMIDAFLWIKLPGE ACGCGCTATCGGAACCCCGAGTACCACAAATACTGGCGACCCAATGADGCIAGAGQFVPQAA ATCGATGCTTTCCTCTGGATTAAGCTTCCGGGAGAAGCAGACGGTTYEMAIAAGGTNPNPNP GCATCGCCGGAGCTGGCCAATTCGTTCCACAAGCAGCATACGAGATNPTPTPTPTPTPPPGS GGCTATTGCGGCGGGTGGTACGAATCCTAATCCCAACCCCAACCCTSGACTATYTIANEWND ACGCCAACGCCCACACCGACTCCCACTCCACCTCCGGGGAGCAGCGGFQATVTVTANQNITG GCGCCTGCACAGCCACCTATACAATCGCAAACGAATGGAATGATGGWTVTWTFTDGQTITNA CTTCCAAGCGACGGTGACGGTGACCGCGAACCAGAACATCACTGGGWNADVSTSGSSVTARN TGGACTGTCACTTGGACTTTCACGGATGGACAGACTATTACTAACGVGHNGTLSQGASTEFG CCTGGAATGCTGACGTTTCGACGTCAGGTTCGTCTGTGACGGCGCGFVGSKGNSNSVPTLTC CAACGTCGGGCATAATGGTACTCTCTCCCAGGGCGCCAGCACAGAGAASSEKDEL* TTTGGCTTTGTCGGCTCAAAGGGAAATTCAAATAGCGTCCCCACTC (SEQ ID NO:82) TCACGTGCGCCGCCTCGAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 154) 2055HvAleSP:P26222 MAHARVLLLALAVLATATGGCCCACGCCCGCGTCCTCCTCCTGGCGCTCGCCGTCCTGGCCA AAVAVASSSSFADSNPCCGCCGCCGTCGCCGTCGCCTCCTCCTCCTCCTTCGCCGACTCCAA IRPVTDRAASTNDSPFCCCGATCCGCCCGGTGACCGACCGCGCCGCCTCCACCAACGATAGT YVNPNMSSAEWVRNNPCCATTCTACGTGAACCCGAATATGTCATCAGCTGAGTGGGTGCGTA NDPRTPVIRDRIASVPACAACCCCAATGACCCTCGCACACCAGTCATTAGGGATCGTATTGC QGTWFAHHNPGQITGQCTCGGTGCCCCAAGGAACGTGGTTCGCCCACCATAACCCTGGCCAG VDALMSAAQAAGKIPIATTACAGGGCAAGTTGATGCTCTGATGTCCGCCGCTCAAGCCGCGG LVVYNAPGRDCGNHSSGTAAGATCCCTATTCTCGTGGTGTACAACGCACCAGGACGCGACTG GGAPSHSAYRSWIDEFCGGGAATCATAGTTCGGGTGGGGCTCCTTCCCACAGCGCTTATCGG AAGLKNRPAYIIVEPDTCTTGGATCGACGAGTTTGCTGCTGGCCTCAAGAACCGTCCCGCTT LISLMSSCMQHVQQEVACATCATTGTGGAGCCTGACCTGATAAGCCTTATGTCGTCGTGTAT LETMAYAGKALKAGSSGCAGCACGTTCAACAGGAGGTGCTCGAGACTATGGCCTACGCAGGG QARIYFDAGHSAWHSPAAGGCCTTGAAGGCCGGCTCATCCCAGGCCCGTATCTATTTCGACG AQMASWLQQADISNSACGGGGCATTCGGCGTGGCATTCACCAGCGCAGATGGCTTCTTGGCT HGIATNTSNYRWTADECCAGCAGGCTGATATCTCAAACTCTGCACATGGTATCGCCACGAAT VAYAKAVLSAIGNPSLACTTCTAACTACCGTTGGACCGCTGATGAAGTCGCGTACGCCAAGG RAVIDTSRNGNGPAGNCCGTGCTGTCCGCCATAGGAAATCCCTCCCTCAGAGCCGTCATAGA EWCDPSGRAIGTPSTTTACGTCCCGCAACGGAAATGGCCCTGCTGGAAATGAGTGGTGCGAC NTGDPMIDAFLWIKLPCCAAGCGGACGCGCTATCGGAACCCCGAGTACCACAAATACTGGCG GEADGCIAGAGQFVPQACCCAATGATCGATGCTTTCCTCTGGATTAAGCTTCCGGGAGAAGC AAYEMAIAAGGTNPNPAGACGGTTGCATCGCCGGAGCTGGCCAATTCGTTCCACAAGCAGCA NPNPTPTPTPTPTPPPTACGAGATGGCTATTGCGGCGGGTGGTACGAATCCTAATCCCAACC GSSGACTATYTIANEWCCAACCCTACGCCAACGCCCACACCGACTCCCACTCCACCTCCGGG NDGFQATVTVTANQNIGAGCAGCGGCGCCTGCACAGCCACCTATACAATCGCAAACGAATGG TGWTVTWTFTDGQTITAATGATGGCTTCCAAGCGACGGTGACGGTGACCGCGAACCAGAACA NAWNADVSTSGSSVTATCACTGGGTGGACTGTCACTTGGACTTTCACGGATGGACAGACTAT RNVGHNGTLSQGASTETACTAACGCCTGGAATGCTGACGTTTCGACGTCAGGTTCGTCTGTG FGFVGSKGNSNSVPTLACGGCGCGCAACGTCGGGCATAATGGTACTCTCTCCCAGGGCGCCA TCAAS* (SEQ IDGCACAGAGTTTGGCTTTGTCGGCTCAAAGGGAAATTCAAATAGCGT NO:CCCCACTCTCACGTGCGCCGCCTCGTGA (SEQ ID NO: 83) 155) 2056 HvAleSP:P26222:MAHARVLLLALAVLAT ATGGCCCACGCCCGCGTCCTCCTCCTGGCGCTCGCCGTCCTGGCCA SEKDELAAVAVASSSSFADSNP CCGCCGCCGTCGCCGTCGCCTCCTCCTCCTCCTTCGCCGACTCCAAIRPVTDRAASTNDSPF CCCGATCCGCCCGGTGACCGACCGCGCCGCCTCCACCAACGATAGTYVNPNMSSAEWVRNNP CCATTCTACGTGAACCCGAATATGTCATCAGCTGAGTGGGTGCGTANDPRTPVIRDRIASVP ACAACCCCAATGACCCTCGCACACCAGTCATTAGGGATCGTATTGCQGTWFAHHNPGQITGQ CTCGGTGCCCCAAGGAACGTGGTTCGCCCACCATAACCCTGGCCAGVDALMSAAQAAGKIPI ATTACAGGGCAAGTTGATGCTCTGATGTCCGCCGCTCAAGCCGCGGLVVYNAPGRDCGNHSS GTAAGATCCCTATTCTCGTGGTGTACAACGCACCAGGACGCGACTGGGAPSHSAYRSWIDEF CGGGAATCATAGTTCGGGTGGGGCTCCTTCCCACAGCGCTTATCGGAAGLKNRPAYIIVEPD TCTTGGATCGACGAGTTTGCTGCTGGCCTCAAGAACCGTCCCGCTTLISLMSSCMQHVQQEV ACATCATTGTGGAGCCTGACCTGATAAGCCTTATGTCGTCGTGTATLETMAYAGKALKAGSS GCAGCACGTTCAACAGGAGGTGCTCGAGACTATGGCCTACGCAGGGQARIYFDAGHSAWHSP AAGGCCTTGAAGGCCGGCTCATCCCAGGCCCGTATCTATTTCGACGAQMASWLQQADISNSA CGGGGCATTCGGCGTGGCATTCACCAGCGCAGATGGCTTCTTGGCTHGIATNTSNYRWTADE CCAGCAGGCTGATATCTCAAACTCTGCACATGGTATCGCCACGAATVAYAKAVLSAIGNPSL ACTTCTAACTACCGTTGGACCGCTGATGAAGTCGCGTACGCCAAGGRAVIDTSRNGNGPAGN CCGTGCTGTCCGCCATAGGAAATCCCTCCCTCAGAGCCGTCATAGAEWCDPSGRAIGTPSTT TACGTCCCGCAACGGAAATGGCCCTGCTGGAAATGAGTGGTGCGACNTGDPMIDAFLWIKLP CCAAGCGGACGCGCTATCGGAACCCCGAGTACCACAAATACTGGCGGEADGCIAGAGQFVPQ ACCCAATGATCGATGCTTTCCTCTGGATTAAGCTTCCGGGAGAAGCAAYEMAIAAGGTNPNP AGACGGTTGCATCGCCGGAGCTGGCCAATTCGTTCCACAAGCAGCANPNPTPTPTPTPTPPP TACGAGATGGCTATTGCGGCGGGTGGTACGAATCCTAATCCCAACCGSSGACTATYTIANEW CCAACCCTACGCCAACGCCCACACCGACTCCCACTCCACCTCCGGGNDGFQATVTVTANQNI GAGCAGCGGCGCCTGCACAGCCACCTATACAATCGCAAACGAATGGTGWTVTWTFTDGQTIT AATGATGGCTTCCAAGCGACGGTGACGGTGACCGCGAACCAGAACANAWNADVSTSGSSVTA TCACTGGGTGGACTGTCACTTGGACTTTCACGGATGGACAGACTATRNVGHNGTLSQGASTE TACTAACGCCTGGAATGCTGACGTTTCGACGTCAGGTTCGTCTGTGFGFVGSKGNSNSVPTL ACGGCGCGCAACGTCGGGCATAATGGTACTCTCTCCCAGGGCGCCATCAASSEKDEL* GCACAGAGTTTGGCTTTGTCGGCTCAAAGGGAAATTCAAATAGCGT (SEQ ID NO:84) CCCCACTCTCACGTGCGCCGCCTCGAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 156) 2057BAASS:P77853:SEKDEL MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQQTSITLTGCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCT SNASGTFDGYYYELWKGACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTC DTGNTTMTVYTQGRFSTGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTC CQWSNINNALFRTGKKGCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGAC YNQNWQSLGTIRITYSCGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGG ATYNPNGNSYLCIYGWATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGT STNPLVEFYIVESWGNGTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGT WRPPGATSLGQVTIDGTGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTGGGC GTYDIYRTTRVNQPSICAAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGACAC VGTATFDQYWSVRTSKGCGTCAACCAGCCTTCCATTGTGGGGACAGCCACGTTCGATCAGTA RTSGTVTVTDHFRAWACTGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAACAGTGACCGTG NRGLNLGTIDQITLCVACCGATCACTTCCGCGCCTGGGCGAACCGGGGCCTGAACCTCGGCA EGYQSSGSANITQNTFCAATAGACCAAATTACATTGTGCGTGGAGGGTTACCAAAGCTCTGG SQGSSSGSSGGSSGSTATCAGCCAACATCACCCAGAACACCTTCTCTCAGGGCTCTTCTTCC TTTRIECENMSLSGPYGGCAGTTCGGGTGGCTCATCCGGCTCCACAACGACTACTCGCATCG VSRITNPFNGIALYANAGTGTGAGAACATGTCCTTGTCCGGACCCTACGTTAGCAGGATCAC GDTARATVNFPASRNYCAATCCCTTTAATGGTATTGCGCTGTACGCCAACGGAGACACAGCC NFRLRGCGNNNNLARVCGCGCTACCGTTAACTTCCCCGCAAGTCGCAACTACAATTTCCGCC DLRIDGRTVGTFYYQGTGCGGGGTTGCGGCAACAACAATAATCTTGCCCGTGTGGACCTGAG TYPWEAPIDNVYVSAGGATCGACGGACGGACCGTCGGGACCTTTTATTACCAGGGCACATAC SHTVEITVTADNGTWDCCCTGGGAGGCCCCAATTGACAATGTTTATGTCAGTGCGGGGAGTC VYADYLVIQSEKDEL*ATACAGTCGAAATCACTGTTACTGCGGATAACGGCACATGGGACGT (SEQ ID NO: 85)GTATGCCGACTACCTGGTGATACAGAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 157) 2058PR1a:P77853:SEKDEL MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT TLLLFLVISHSCRAQQCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCCA TSITLTSNASGTFDGYGCAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTGAC YYELWKDTGNTTMTVYGGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAATGA TQGRFSCQWSNINNALCGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCGAACATCAA FRTGKKYNQNWQSLGTTAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTGGCAG IRITYSATYNPNGNSYTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAACCCAA LCIYGWSTNPLVEFYIACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACCCATT VESWGNWRPPGATSLGGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACCGCCT QVTIDGGTYDIYRTTRGGTGCCACGTCCCTGGGCCAAGTGACAATCGATGGCGGGACCTACG VNQPSIVGTATFDQYWACATCTATAGGACGACACGCGTCAACCAGCCTTCCATTGTGGGGAC SVRTSKRTSGTVTVTDAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCTAAGCGGACT HFRAWANRGLNLGTIDTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCTGGGCGAACC QITLCVEGYQSSGSANGGGGCCTGAACCTCGGCACAATAGACCAAATTACATTGTGCGTGGA ITQNTFSQGSSSGSSGGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAGAACACCTTC GSSGSTTTTRIECENMTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCATCCGGCTCCA SLSGPYVSRITNPFNGCAACGACTACTCGCATCGAGTGTGAGAACATGTCCTTGTCCGGACC IALYANGDTARATVNFCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATTGCGCTGTAC PASRNYNFRLRGCGNNGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCCCCGCAAGTC NNLARVDLRIDGRTVGGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAACAATAATCT TFYYQGTYPWEAPIDNTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTCGGGACCTTT VYVSAGSHTVEITVTATATTACCAGGGCACATACCCCTGGGAGGCCCCAATTGACAATGTTT DNGTWDVYADYLVIQSATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGTTACTGCGGA EKDEL* (SEQ IDTAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACAGAGC NO: 86)GAGAAGGACGAGCTGTGA (SEQ ID NO: 158) 2059 O43097 MFPAGNATELEKRQTTATGTTCCCAGCTGGAAACGCAACGGAATTGGAGAAAAGACAAACCA PNSEGWHDGYYYSWWSCCCCTAACTCTGAGGGCTGGCATGACGGATACTACTACTCTTGGTG DGGAQATYTNLEGGTYGAGCGATGGTGGTGCACAGGCCACCTATACAAACCTCGAAGGCGGC EISWGDGGNLVGGKGWACTTATGAGATTTCATGGGGTGACGGTGGCAACCTTGTCGGCGGAA NPGLNARAIHFEGVYQAGGGGTGGAACCCCGGACTTAACGCCAGGGCAATCCACTTCGAAGG PNGNSYLAVYGWTRNPGGTGTACCAGCCCAATGGCAACTCATACCTGGCCGTCTACGGGTGG LVEYYIVENFGTYDPSACGCGCAATCCGCTGGTTGAGTACTATATCGTGGAGAATTTCGGAA SGATDLGTVECDGSIYCTTATGACCCTAGCTCCGGTGCCACGGACCTCGGGACAGTCGAGTG RLGKTTRVNAPSIDGTTGACGGAAGCATCTACAGGCTGGGTAAAACTACCCGCGTTAATGCT QTFDQYWSVRQDKRTSCCATCGATCGACGGCACGCAAACATTTGATCAATACTGGTCCGTGC GTVQTGCHFDAWARAGGGCAGGATAAGAGGACAAGCGGCACAGTTCAGACGGGTTGCCACTT LNVNGDHYYQIVATEGTGATGCCTGGGCAAGAGCGGGGCTCAATGTGAATGGGGACCACTAC YFSSGYARITVADVG*TATCAGATTGTGGCGACCGAGGGCTATTTCTCCAGTGGCTATGCGC (SEQ ID NO: 87)GTATAACCGTCGCTGATGTTGGATGA (SEQ ID NO: 159) 2060 PR1a:O43097MGFVLFSQLPSFLLVS ATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCTTLLLFLVISHSCRAFP CCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCTTAGNATELEKRQTTPNS CCCAGCTGGAAACGCAACGGAATTGGAGAAAAGACAAACCACCCCTEGWHDGYYYSWWSDGG AACTCTGAGGGCTGGCATGACGGATACTACTACTCTTGGTGGAGCGAQATYTNLEGGTYEIS ATGGTGGTGCACAGGCCACCTATACAAACCTCGAAGGCGGCACTTAWGDGGNLVGGKGWNPG TGAGATTTCATGGGGTGACGGTGGCAACCTTGTCGGCGGAAAGGGGLNARAIHFEGVYQPNG TGGAACCCCGGACTTAACGCCAGGGCAATCCACTTCGAAGGGGTGTNSYLAVYGWTRNPLVE ACCAGCCCAATGGCAACTCATACCTGGCCGTCTACGGGTGGACGCGYYIVENFGTYDPSSGA CAATCCGCTGGTTGAGTACTATATCGTGGAGAATTTCGGAACTTATTDLGTVECDGSIYRLG GACCCTAGCTCCGGTGCCACGGACCTCGGGACAGTCGAGTGTGACGKTTRVNAPSIDGTQTF GAAGCATCTACAGGCTGGGTAAAACTACCCGCGTTAATGCTCCATCDQYWSVRQDKRTSGTV GATCGACGGCACGCAAACATTTGATCAATACTGGTCCGTGCGGCAGQTGCHFDAWARAGLNV GATAAGAGGACAAGCGGCACAGTTCAGACGGGTTGCCACTTTGATGNGDHYYQIVATEGYFS CCTGGGCAAGAGCGGGGCTCAATGTGAATGGGGACCACTACTATCASGYARITVADVG* GATTGTGGCGACCGAGGGCTATTTCTCCAGTGGCTATGCGCGTATA (SEQ ID NO:88) ACCGTCGCTGATGTTGGATGA (SEQ ID NO: 160) 2061 PR1a:O43097:SEKDELMGFVLFSQLPSFLLVS ATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCTTLLLFLVISHSCRAFP CCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCTTAGNATELEKRQTTPNS CCCAGCTGGAAACGCAACGGAATTGGAGAAAAGACAAACCACCCCTEGWHDGYYYSWWSDGG AACTCTGAGGGCTGGCATGACGGATACTACTACTCTTGGTGGAGCGAQATYTNLEGGTYEIS ATGGTGGTGCACAGGCCACCTATACAAACCTCGAAGGCGGCACTTAWGDGGNLVGGKGWNPG TGAGATTTCATGGGGTGACGGTGGCAACCTTGTCGGCGGAAAGGGGLNARAIHFEGVYQPNG TGGAACCCCGGACTTAACGCCAGGGCAATCCACTTCGAAGGGGTGTNSYLAVYGWTRNPLVE ACCAGCCCAATGGCAACTCATACCTGGCCGTCTACGGGTGGACGCGYYIVENFGTYDPSSGA CAATCCGCTGGTTGAGTACTATATCGTGGAGAATTTCGGAACTTATTDLGTVECDGSIYRLG GACCCTAGCTCCGGTGCCACGGACCTCGGGACAGTCGAGTGTGACGKTTRVNAPSIDGTQTF GAAGCATCTACAGGCTGGGTAAAACTACCCGCGTTAATGCTCCATCDQYWSVRQDKRTSGTV GATCGACGGCACGCAAACATTTGATCAATACTGGTCCGTGCGGCAGQTGCHFDAWARAGLNV GATAAGAGGACAAGCGGCACAGTTCAGACGGGTTGCCACTTTGATGNGDHYYQIVATEGYFS CCTGGGCAAGAGCGGGGCTCAATGTGAATGGGGACCACTACTATCASGYARITVADVGSEKD GATTGTGGCGACCGAGGGCTATTTCTCCAGTGGCTATGCGCGTATA EL* (SEQID ACCGTCGCTGATGTTGGAAGCGAGAAGGACGAGCTGTGA (SEQ NO: 89) ID NO: 161) 2062BAASS:O43097 MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQVFPAGNAGCCTGTCGGCCAGCTTGGCCTCCGGGCAAGTCTTCCCAGCTGGAAA TELEKRQTTPNSEGWHCGCAACGGAATTGGAGAAAAGACAAACCACCCCTAACTCTGAGGGC DGYYYSWWSDGGAQATTGGCATGACGGATACTACTACTCTTGGTGGAGCGATGGTGGTGCAC YTNLEGGTYEISWGDGAGGCCACCTATACAAACCTCGAAGGCGGCACTTATGAGATTTCATG GNLVGGKGWNPGLNARGGGTGACGGTGGCAACCTTGTCGGCGGAAAGGGGTGGAACCCCGGA AIHFEGVYQPNGNSYLCTTAACGCCAGGGCAATCCACTTCGAAGGGGTGTACCAGCCCAATG AVYGWTRNPLVEYYIVGCAACTCATACCTGGCCGTCTACGGGTGGACGCGCAATCCGCTGGT ENFGTYDPSSGATDLGTGAGTACTATATCGTGGAGAATTTCGGAACTTATGACCCTAGCTCC TVECDGSIYRLGKTTRGGTGCCACGGACCTCGGGACAGTCGAGTGTGACGGAAGCATCTACA VNAPSIDGTQTFDQYWGGCTGGGTAAAACTACCCGCGTTAATGCTCCATCGATCGACGGCAC SVRQDKRTSGTVQTGCGCAAACATTTGATCAATACTGGTCCGTGCGGCAGGATAAGAGGACA HFDAWARAGLNVNGDHAGCGGCACAGTTCAGACGGGTTGCCACTTTGATGCCTGGGCAAGAG YYQIVATEGYFSSGYACGGGGCTCAATGTGAATGGGGACCACTACTATCAGATTGTGGCGAC RITVADVG* (SEQCGAGGGCTATTTCTCCAGTGGCTATGCGCGTATAACCGTCGCTGAT ID NO: 90) GTTGGATGA (SEQID NO: 162) 2063 BAASS:O43097:SEKDEL MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQVFPAGNAGCCTGTCGGCCAGCTTGGCCTCCGGGCAAGTCTTCCCAGCTGGAAA TELEKRQTTPNSEGWHCGCAACGGAATTGGAGAAAAGACAAACCACCCCTAACTCTGAGGGC DGYYYSWWSDGGAQATTGGCATGACGGATACTACTACTCTTGGTGGAGCGATGGTGGTGCAC YTNLEGGTYEISWGDGAGGCCACCTATACAAACCTCGAAGGCGGCACTTATGAGATTTCATG GNLVGGKGWNPGLNARGGGTGACGGTGGCAACCTTGTCGGCGGAAAGGGGTGGAACCCCGGA AIHFEGVYQPNGNSYLCTTAACGCCAGGGCAATCCACTTCGAAGGGGTGTACCAGCCCAATG AVYGWTRNPLVEYYIVGCAACTCATACCTGGCCGTCTACGGGTGGACGCGCAATCCGCTGGT ENFGTYDPSSGATDLGTGAGTACTATATCGTGGAGAATTTCGGAACTTATGACCCTAGCTCC TVECDGSIYRLGKTTRGGTGCCACGGACCTCGGGACAGTCGAGTGTGACGGAAGCATCTACA VNAPSIDGTQTFDQYWGGCTGGGTAAAACTACCCGCGTTAATGCTCCATCGATCGACGGCAC SVRQDKRTSGTVQTGCGCAAACATTTGATCAATACTGGTCCGTGCGGCAGGATAAGAGGACA HFDAWARAGLNVNGDHAGCGGCACAGTTCAGACGGGTTGCCACTTTGATGCCTGGGCAAGAG YYQIVATEGYFSSGYACGGGGCTCAATGTGAATGGGGACCACTACTATCAGATTGTGGCGAC RITVADVGSEKDEL*CGAGGGCTATTTCTCCAGTGGCTATGCGCGTATAACCGTCGCTGAT (SEQ ID NO: 91)GTTGGAAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 163) 2064 HvAleSP:O43097MAHARVLLLALAVLAT ATGGCCCACGCCCGCGTCCTCCTCCTGGCGCTCGCCGTCCTGGCCAAAVAVASSSSFADSNP CCGCCGCCGTCGCCGTCGCCTCCTCCTCCTCCTTCGCCGACTCCAAIRPVTDRAASTFPAGN CCCGATCCGCCCGGTGACCGACCGCGCCGCCTCCACCTTCCCAGCTATELEKRQTTPNSEGW GGAAACGCAACGGAATTGGAGAAAAGACAAACCACCCCTAACTCTGHDGYYYSWWSDGGAQA AGGGCTGGCATGACGGATACTACTACTCTTGGTGGAGCGATGGTGGTYTNLEGGTYEISWGD TGCACAGGCCACCTATACAAACCTCGAAGGCGGCACTTATGAGATTGGNLVGGKGWNPGLNA TCATGGGGTGACGGTGGCAACCTTGTCGGCGGAAAGGGGTGGAACCRAIHFEGVYQPNGNSY CCGGACTTAACGCCAGGGCAATCCACTTCGAAGGGGTGTACCAGCCLAVYGWTRNPLVEYYI CAATGGCAACTCATACCTGGCCGTCTACGGGTGGACGCGCAATCCGVENFGTYDPSSGATDL CTGGTTGAGTACTATATCGTGGAGAATTTCGGAACTTATGACCCTAGTVECDGSIYRLGKTT GCTCCGGTGCCACGGACCTCGGGACAGTCGAGTGTGACGGAAGCATRVNAPSIDGTQTFDQY CTACAGGCTGGGTAAAACTACCCGCGTTAATGCTCCATCGATCGACWSVRQDKRTSGTVQTG GGCACGCAAACATTTGATCAATACTGGTCCGTGCGGCAGGATAAGACHFDAWARAGLNVNGD GGACAAGCGGCACAGTTCAGACGGGTTGCCACTTTGATGCCTGGGCHYYQIVATEGYFSSGY AAGAGCGGGGCTCAATGTGAATGGGGACCACTACTATCAGATTGTGARITVADVG* GCGACCGAGGGCTATTTCTCCAGTGGCTATGCGCGTATAACCGTCG (SEQ ID NO:92) CTGATGTTGGATGA (SEQ ID NO: 164) 2065 HvAleSP:O43097:MAHARVLLLALAVLAT ATGGCCCACGCCCGCGTCCTCCTCCTGGCGCTCGCCGTCCTGGCCA SEKDELAAVAVASSSSFADSNP CCGCCGCCGTCGCCGTCGCCTCCTCCTCCTCCTTCGCCGACTCCAAIRPVTDRAASTFPAGN CCCGATCCGCCCGGTGACCGACCGCGCCGCCTCCACCTTCCCAGCTATELEKRQTTPNSEGW GGAAACGCAACGGAATTGGAGAAAAGACAAACCACCCCTAACTCTGHDGYYYSWWSDGGAQA AGGGCTGGCATGACGGATACTACTACTCTTGGTGGAGCGATGGTGGTYTNLEGGTYEISWGD TGCACAGGCCACCTATACAAACCTCGAAGGCGGCACTTATGAGATTGGNLVGGKGWNPGLNA TCATGGGGTGACGGTGGCAACCTTGTCGGCGGAAAGGGGTGGAACCRAIHFEGVYQPNGNSY CCGGACTTAACGCCAGGGCAATCCACTTCGAAGGGGTGTACCAGCCLAVYGWTRNPLVEYYI CAATGGCAACTCATACCTGGCCGTCTACGGGTGGACGCGCAATCCGVENFGTYDPSSGATDL CTGGTTGAGTACTATATCGTGGAGAATTTCGGAACTTATGACCCTAGTVECDGSIYRLGKTT GCTCCGGTGCCACGGACCTCGGGACAGTCGAGTGTGACGGAAGCATRVNAPSIDGTQTFDQY CTACAGGCTGGGTAAAACTACCCGCGTTAATGCTCCATCGATCGACWSVRQDKRTSGTVQTG GGCACGCAAACATTTGATCAATACTGGTCCGTGCGGCAGGATAAGACHFDAWARAGLNVNGD GGACAAGCGGCACAGTTCAGACGGGTTGCCACTTTGATGCCTGGGCHYYQIVATEGYFSSGY AAGAGCGGGGCTCAATGTGAATGGGGACCACTACTATCAGATTGTGARITVADVGSEKDEL* GCGACCGAGGGCTATTTCTCCAGTGGCTATGCGCGTATAACCGTCG (SEQ IDNO: 93) CTGATGTTGGAAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 165) 2066BAASS:P77853S158-2 MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQQTSITLTGCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCT SNASGTFDGYYYELWKGACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTC DTGNTTMTVYTQGRFSTGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTC CQWSNINNALFRTGKKGCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGAC YNQNWQSLGTIRITYSCGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGG ATYNPNGNSYLCIYGWATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGT STNPLVEFYIVESWGNGTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGT WRPPGATSLGQVTIDGTGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTGGGC GTYDIYRTTRVNQPCLCAAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGACAC AEGSLVLDAATGQRVPGCGTCAACCAGCCTTGCCTGGCCGAGGGCTCGCTCGTCTTGGACGC IEKVRPGMEVFSLGPDGGCTACCGGGCAGAGGGTCCCTATCGAAAAGGTGCGTCCGGGGATG YRLYRVPVLEVLESGVGAAGTTTTCTCCTTGGGACCTGATTACAGACTGTATCGGGTGCCCG REVVRLRTRSGRTLVLTTTTGGAGGTCCTTGAGAGCGGGGTTAGGGAAGTTGTGCGCCTCAG TPDHPLLTPEGWKPLCAACTCGGTCAGGGAGAACGCTGGTGTTGACACCAGATCACCCGCTT DLPLGTPIAVPAELPVTTGACCCCCGAAGGTTGGAAACCTCTTTGTGACCTCCCGCTTGGAA AGHLAPPEERVTLLALCTCCAATTGCAGTCCCCGCAGAACTGCCTGTGGCGGGCCACTTGGC LLGDGNTKLPGRRGTRCCCACCTGAAGAACGTGTTACGCTCCTGGCTCTTCTGTTGGGGGAT PNAFFYSKDPELLAAYGGGAACACAAAGCTGCCGGGTCGGAGAGGTACACGTCCTAATGCCT RRCAEALGAKVKAYVHTCTTCTACAGCAAAGACCCCGAATTGCTCGCGGCTTATCGCCGGTG PTTGVVTLATLAPRPGTGCAGAAGCCTTGGGTGCAAAGGTGAAAGCATACGTCCACCCGACT AQDPVKRLVVEAGMVAACGGGGGTGGTTACACTCGCAACCCTCGCTCCACGTCCTGGAGCTC KAEEKRVPEEVFRYRRAAGATCCTGTCAAACGCCTCGTTGTCGAGGCGGGAATGGTTGCTAA EALALFLGRLFSTDGSAGCCGAAGAGAAGAGGGTCCCGGAGGAGGTGTTTCGTTACCGGCGT VEKKRISYSSASLGLAGAGGCGTTGGCCCTTTTCTTGGGCCGTTTGTTCTCGACAGACGGCT QDDAHLLLRLGITSQLCTGTTGAAAAGAAGAGGATCTCTTATTCAAGTGCCAGTTTGGGACT RSRGPRAHEVLISGREGGCCCAGGATGACGCACATCTCTTGCTGCGCCTTGGAATTACATCT DILRFAELIGPYLLGACAACTCCGTTCGAGAGGGCCACGGGCTCACGAGGTTCTTATATCGG KRERLAALEAEARRRLGCCGCGAGGATATTTTGCGGTTTGCTGAACTTATCGGACCCTACCT PGQGWHLRLVLPAVAYCTTGGGGGCCAAGAGGGAGAGACTTGCAGCGCTGGAAGCTGAGGCC RVSEAKRRSGFSWSEACGCAGGCGTTTGCCTGGACAGGGATGGCACTTGCGGCTTGTTCTTC GRRVAVAGSCLSSGLNCTGCCGTGGCGTACAGAGTGAGCGAGGCTAAAAGGCGCTCGGGATT LKLPRRYLSRHRLSLLTTCGTGGAGTGAAGCCGGTCGGCGCGTCGCAGTTGCGGGATCGTGT GEAFADPGLEALAEGQTTGTCATCTGGACTCAACCTCAAATTGCCCAGACGCTACCTTTCTC VLWDPIVAVEPAGKARGGCACCGGTTGTCGCTGCTCGGTGAGGCTTTTGCCGACCCTGGGCT TFDLRVPPFANFVSEDGGAAGCGCTCGCGGAAGGCCAAGTGCTCTGGGACCCTATTGTTGCT LVVHNSIVGTATFDQYGTCGAACCGGCCGGTAAGGCGAGAACATTCGACTTGCGCGTTCCAC WSVRTSKRTSGTVTVTCCTTTGCAAACTTCGTGAGCGAGGACCTGGTGGTGCATAACTCCAT DHFRAWANRGLNLGTITGTGGGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCT DQITLCVEGYQSSGSAAAGCGGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCT NITQNTFSQGSSSGSSGGGCGAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTACATT GGSSGSTTTTRIECENGTGCGTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAG MSLSGPYVSRITNPFNAACACCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCAT GIALYANGDTARATVNCCGGCTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTCCTT FPASRNYNFRLRGCGNGTCCGGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATT NNNLARVDLRIDGRTVGCGCTGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCC GTFYYQGTYPWEAPIDCCGCAAGTCGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAA NVYVSAGSHTVEITVTCAATAATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTC ADNGTWDVYADYLVIQGGGACCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAATTG * (SEQ ID NO:ACAATGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGT 94)TACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTG ATACAGTGA (SEQ ID NO:166) 2067 BAASS:P77853S158- MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG 19 LSASLASGQQTSITLTGCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCT SNASGTFDGYYYELWKGACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTC DTGNTTMTVYTQGRFSTGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTC CQWSNINNALFRTGKKGCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGAC YNQNWQSLGTIRITYSCGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGG ATYNPNGNSYLCIYGWATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGT STNPLVEFYIVESWGNGTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGT WRPPGATSLGQVTIDGTGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTGGGC GTYDIYRTTRVNQPCLCAAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGACAC AEGSLVLDAATGQRVPGCGTCAACCAGCCTTGCCTGGCCGAGGGCTCGCTCGTCTTGGACGC IEKVRPGMEVFSLGPDGGCTACCGGGCAGAGGGTCCCTATCGAAAAGGTGCGTCCGGGGATG YRLYRVPVLEVLESGVGAAGTTTTCTCCTTGGGACCTGATTACAGACTGTATCGGGTGCCCG GEVVRLRTRSGRTLVLTTTTGGAGGTCCTTGAGAGCGGGGTTGGGGAAGTTGTGCGCGTCAG TPDHPLLTPEGWKPLCAACTCGGTCAGGGAGAACGCTGGTGTTGACACCAGATCACCCGCTT DLPLGTPIAVPAELPVTTGACCCCCGAAGGTTGGAAACCTGTTTGTGACCTCCCGCTTGGAA AGHLAPPEERVTLLALCTCCAATTGCAGTCCCCGCAGAACTGCCTGTGGCGGGCCACTTGGC LLGDGNTKLSGRRGTRCCCACCTGAAGAACGTGTTACGCTCCTGGCTCTTCTGTTGGGGGAT PIAFFYSKDPELLAAYGGGAACACAAAGCTGTCGGGTCGGAGAGGTACACGTCCTATTGCCT RRCAEALGAKVKAYVHTCTTCTACAGCAAAGACCCCGAATTGCTCGCGGCTTATCGCCGGTG PTTGVVTLATLAPRPGTGCAGAAGCCTTGGGTGCAAAGGTGAAAGCATACGTCCACCCGACT AQDPVKRLVVEAGMVAACGGGGGTGGTTACACTCGCAACCCTCGCTCCACGTCCTGGAGCTC KAEEKRVPEEVFRYRRAAGATCCTGTCAAACGCCTCGTTGTCGAGGCGGGAATGGTTGCTAA EALALFLGRLFSTDGSAGCCGAAGAGAAGAGGGTCCCGGAGGAGGTGTTTCGTTACCGGCGT VEKKRISYSSASLGLAGAGGCGTTGGCCCTTTTCTTGGGCCGTTTGTTCTCGACAGACGGCT QDVAHLLLRLGITSQLCTGTTGAAAAGAAGAGGATCTCTTATTCAAGTGCCAGTTTGGGACT RSRGPRAHEVLISGREGGCCCAGGATGTCGCACATCTCTTGCTGCGCCTTGGAATTACATCT DILRFAELIGPYLLGACAACTCCGTTCGAGAGGGCCACGGGCTCACGAGGTTCTTATATCGG KRERLAALEAEARRRLGCCGCGAGGATATTTTGCGGTTTGCTGAACTTATCGGACCCTACCT PGQGWHLRLVLPAVAYCTTGGGGGCCAAGAGGGAGAGACTTGCAGCGCTGGAAGCTGAGGCC RVSEAKRRSGFSWSEACGCAGGCGTTTGCCTGGACAGGGATGGCACTTGCGGCTTGTTCTTC GRRVAVAGSCLSSGLNCTGCCGTGGCGTACAGAGTGAGCGAGGCTAAAAGGCGCTCGGGATT LKLPRRYLSRHRLSLLTTCGTGGAGTGAAGCCGGTCGGCGCGTCGCAGTTGCGGGATCGTGT GEAFADPGLEALAEGQTTGTCATCTGGACTCAACCTCAAATTGCCCAGACGCTACCTTTCTC VLWDPIVAVEPAGKARGGCACCGGTTGTCGCTGCTCGGTGAGGCTTTTGCCGACCCTGGGCT TFDLRVPPFANFVSEDGGAAGCGCTCGCGGAAGGCCAAGTGCTCTGGGACCCTATTGTTGCT LVVHNSIVGTATFDQYGTCGAACCGGCCGGTAAGGCGAGAACATTCGACTTGCGCGTTCCAC WSVRTSKRTSGTVTVTCCTTTGCAAACTTCGTGAGCGAGGACCTGGTGGTGCATAACTCCAT DHFRAWANRGLNLGTITGTGGGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCT DQITLCVEGYQSSGSAAAGCGGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCT NITQNTFSQGSSSGSSGGGCGAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTACATT GGSSGSTTTTRIECENGTGCGTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAG MSLSGPYVSRITNPFNAACACCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCAT GIALYANGDTARATVNCCGGCTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTCCTT FPASRNYNFRLRGCGNGTCCGGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATT NNNLARVDLRIDGRTVGCGCTGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCC GTFYYQGTYPWEAPIDCCGCAAGTCGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAA NVYVSAGSHTVEITVTCAATAATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTC ADNGTWDVYADYLVIQGGGACCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAATTG * (SEQ ID NO:ACAATGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGT 95)TACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTG ATACAGTGA (SEQ ID NO:167) 2068 BAASS:P77853T134-1 MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQQTSITLTGCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCT SNASGTFDGYYYELWKGACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTC DTGNTTMTVYTQGRFSTGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTC CQWSNINNALFRTGKKGCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGAC YNQNWQSLGTIRITYSCGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGG ATYNPNGNSYLCIYGWATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGT STNPLVEFYIVESWGNGTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGT WRPPGACLAEGSLVLDTGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCTGCCTGGCCGAG AATGQRVPIEKVRPGMGGCTCGCTCGTCTTGGACGCGGCTACCGGGCAGAGGGTCCCTATCG EVFSLGPDYRLYRVPVAAAAGGTGCGTCCGGGGATGGAAGTTTTCTCCTTGGGACCTGATTA LEVLESGVREVVRLRTCAGACTGTATCGGGTGCCCGTTTTGGAGGTCCTTGAGAGCGGGGTT RSGRTLVLTPDHPLLTAGGGAAGTTGTGCGCCTCAGAACTCGGTCAGGGAGAACGCTGGTGT PEGWKPLCDLPLGTPITGACACCAGATCACCCGCTTTTGACCCCCGAAGGTTGGAAACCTCT AVPAELPVACHLAPPETTGTGACCTCCCGCTTGGAACTCCAATTGCAGTCCCCGCAGAACTG ERVTLLALLLGDGNTKCCTGTGGCGTGCCACTTGGCCCCACCTGAAGAACGTGTTACGCTCC PSGRRGTRPNAFFYSKTGGCTCTTCTGTTGGGGGATGGGAACACAAAGCCGTCGGGTCGGAG DPELLAAYRRCAEALGAGGTACACGTCCTAATGCCTTCTTCTACAGCAAAGACCCCGAATTG AKVKAYVHPTTGVVTLCTCGCGGCTTATCGCCGGTGTGCAGAAGCCTTGGGTGCAAAGGTGA ATLAPRPGAQDPVKRLAAGCATACGTCCACCCGACTACGGGGGTGGTTACACTCGCAACCCT VVEAGMVAKAEEKRVPCGCTCCACGTCCTGGAGCTCAAGATCCTGTCAAACGCCTCGTTGTC EEVFRYRREALALFLGGAGGCGGGAATGGTTGCTAAAGCCGAAGAGAAGAGGGTCCCGGAGG RLFSTDGSVEKKRISYAGGTGTTTCGTTACCGGCGTGAGGCGTTGGCCCTTTTCTTGGGCCG SSASLGLAQDVAHLLLTTTGTTCTCGACAGACGGCTCTGTTGAAAAGAAGAGGATCTCTTAT RLGITSQLRSRGPRAHTCAAGTGCCAGTTTGGGACTGGCCCAGGATGTCGCACATCTCTTGC EVLISGREDILRFAELTGCGCCTTGGAATTACATCTCAACTCCGTTCGAGAGGGCCACGGGC IGPYLLGAKRERLAALTCACGAGGTTCTTATATCGGGCCGCGAGGATATTTTGCGGTTTGCT EAEARRRLPGQGWHLRGAACTTATCGGACCCTACCTCTTGGGGGCCAAGAGGGAGAGACTTG LVLPAVAYRVSEAKRRCAGCGCTGGAAGCTGAGGCCCGCAGGCGTTTGCCTGGACAGGGATG SGFSWSEAGRRVAVAGGCACTTGCGGCTTGTTCTTCCTGCCGTGGCGTACAGAGTGAGCGAG SCLSSGLNLKLPRRYLGCTAAAAGGCGCTCGGGATTTTCGTGGAGTGAAGCCGGTCGGCGCG SRHRLSLLGEAFADPGTCGCAGTTGCGGGATCGTGTTTGTCATCTGGACTCAACCTCAAATT LEALAEGQVLWDPIVAGCCCAGACGCTACCTTTCTCGGCACCGGTTGTCGCTGCTCGGTGAG VEPAGKARTFDLRVPPGCTTTTGCCGACCCTGGGCTGGAAGCGCTCGCGGAAGGCCAAGTGC FANFVSEDLVVHNTSPTCTGGGACCCTATTGTTGCTGTCGAACCGGCCGGTAAGGCGAGAAC LGQVTIDGGTYDIYRTATTCGACTTGCGCGTTCCACCCTTTGCAAACTTCGTGAGCGAGGAC TRVNQPSIVGTATFDQCTGGTGGTGCATAACACGTCCCCCTTGGGCCAAGTGACAATCGATG YWSVRTSKRTSGTVTVGCGGGACCTACGACATCTATAGGACGACACGCGTCAACCAGCCTTC TDHFRAWANRGLNLGTCATTGTGGGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACC IDQITLCVEGYQSSGSTCTAAGCGGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCG ANITQNTFSQGSSSGSCCTGGGCGAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTAC SGGSSGSTTTTRIECEATTGTGCGTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACC NMSLSGPYVSRITNPFCAGAACACCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCT NGIALYANGDTARATVCATCCGGCTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTC NFPASRNYNFRLRGCGCTTGTCCGGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGT NNNNLARVDLRIDGRTATTGCGCTGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACT VGTFYYQGTYPWEAPITCCCCGCAAGTCGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAA DNVYVSAGSHTVEITVCAACAATAATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACC TADNGTWDVYADYLVIGTCGGGACCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAA Q* (SEQ ID NO:TTGACAATGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCAC 96)TGTTACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTG GTGATACAGTGA (SEQ ID NO:168) 2069 O68438 MLEDKSPKLPDYKNDLATGCTGGAGGACAAGTCTCCCAAACTGCCTGATTATAAGAACGACC LYERTFDEGLCFPWHTTTCTGTACGAACGCACATTCGACGAGGGGCTCTGCTTCCCGTGGCA CEDSGGKCDFAVVDVPCACGTGCGAAGATTCAGGAGGGAAATGCGATTTTGCCGTGGTCGAC GEPGNKAFRLTVIDKGGTTCCAGGCGAGCCTGGGAACAAGGCGTTCAGGCTCACTGTTATCG QNKWSVQMRHRGITLEATAAGGGTCAGAACAAGTGGTCGGTCCAAATGAGACACCGGGGTAT QGHTYTVRFTIWSDKSCACGTTGGAGCAGGGGCACACATACACCGTTCGGTTTACTATCTGG CRVYAKIGQMGEPYTEAGCGACAAGAGCTGCCGCGTGTATGCCAAAATCGGCCAAATGGGTG YWNNNWNPFNLTPGQKAACCCTACACGGAGTACTGGAACAATAACTGGAATCCGTTCAACCT LTVEQNFTMNYPTDDTCACTCCGGGGCAGAAATTGACGGTGGAACAGAACTTTACTATGAAT CEFTFHLGGELAAGTPTATCCCACGGACGACACGTGTGAGTTTACCTTCCACTTGGGAGGGG YYVYLDDVSLYDPRFVAACTGGCAGCCGGGACCCCTTACTACGTGTACCTCGACGACGTTTC KPVEYVLPQPDVRVNQTCTTTACGATCCCCGCTTTGTCAAGCCAGTGGAATACGTCCTGCCT VGYLPFAKKYATVVSSCAACCGGATGTCAGGGTTAATCAAGTTGGATACCTCCCTTTTGCTA STSPLKWQLLNSANQVAGAAATATGCTACTGTCGTGTCATCGAGCACGTCCCCATTGAAGTG VLEGNTIPKGLDKDSQGCAACTTCTGAATAGTGCAAACCAAGTTGTCTTGGAGGGCAATACA DYVHWIDFSNFKTEGKATCCCCAAGGGACTGGACAAAGATTCACAAGACTACGTTCATTGGA GYYFKLPTVNSDTNYSTCGATTTCTCGAACTTTAAGACCGAAGGCAAGGGGTACTATTTCAA HPFDISADIYSKMKFDGTTGCCCACTGTGAACTCCGATACTAACTACTCCCACCCGTTTGAT ALAFFYHKRSGIPIEMATTTCTGCAGATATCTATTCAAAGATGAAGTTCGACGCGCTCGCTT PYAGGEQWTRPAGHIGTCTTTTACCATAAAAGGTCGGGAATACCAATCGAGATGCCCTACGC VAPNKGDTNVPTWPQDCGGGGGAGAGCAGTGGACAAGGCCCGCAGGGCACATTGGTGTCGCG DEYAGRPQKYYTKDVTCCGAACAAGGGCGACACGAATGTGCCAACTTGGCCCCAGGATGACG GGWYDAGDHGKYVVNGAATATGCTGGACGCCCCCAGAAATACTATACGAAAGACGTGACCGG GIAVWTLMNMYERAKICGGGTGGTACGATGCCGGTGACCACGGCAAGTACGTCGTGAACGGG RGIANQGAYKDGGMNIGGTATCGCAGTTTGGACCCTTATGAATATGTACGAGAGAGCAAAGA PERNNGYPDILDEARWTTAGAGGAATCGCTAACCAGGGTGCCTACAAAGATGGAGGAATGAA EIEFFKKMQVTEKEDPTATCCCGGAAAGGAATAACGGCTATCCTGATATTCTGGACGAGGCC SIAGMVHHKIHDFRWTAGATGGGAGATCGAATTTTTTAAGAAGATGCAAGTCACTGAGAAAG ALGMLPHEDPQPRYLRAAGATCCGTCGATTGCAGGTATGGTGCACCACAAGATCCACGATTT PVSTAATLNFAATLAQCAGGTGGACGGCGCTCGGAATGTTGCCTCACGAGGACCCCCAGCCA SARLWKDYDPTFAADCCGCTACCTTCGGCCCGTCAGCACAGCGGCAACCCTGAATTTCGCAG LEKAEIAWQAALKHPDCGACCCTCGCTCAGTCTGCCAGATTGTGGAAGGATTACGACCCGAC IYAEYTPGSGGPGGGPTTTTGCAGCGGACTGCCTTGAGAAAGCTGAAATTGCCTGGCAAGCA YNDDYVGDEFYWAACEGCACTCAAACACCCGGACATCTACGCTGAGTACACGCCAGGAAGCG LYVTTGKDEYKNYLMNGTGGGCCGGGTGGAGGTCCTTATAATGACGATTATGTCGGGGACGA SPHYLEMPAKMGENGGGTTCTACTGGGCCGCTTGTGAACTCTATGTGACAACCGGTAAGGAT ANGEDNGLWGCFTWGTGAGTACAAGAATTACTTGATGAATAGTCCGCACTATCTGGAAATGC TQGLGTITLALVENGLCAGCGAAGATGGGCGAGAACGGAGGGGCTAACGGCGAGGACAACGG PSADIQKARNNIAKAATCTCTGGGGCTGCTTTACTTGGGGAACGACACAGGGGTTGGGTACA DKWLENIEEQGYRLPIATTACCCTTGCCCTCGTTGAAAACGGCCTCCCTTCGGCGGATATTC KQAEDERGGYPWGSNSAAAAGGCCCGCAACAATATCGCTAAAGCCGCAGATAAGTGGCTTGA FILNQMIVMGYAYDFTGAATATTGAAGAACAAGGTTACCGCCTGCCTATCAAACAAGCGGAG GNSKYLDGMQDGMSYLGATGAACGGGGCGGATACCCGTGGGGTAGTAATTCTTTCATTCTCA LGRNGLDQSYVTGYGEACCAGATGATCGTCATGGGCTACGCTTACGACTTCACGGGAAACAG RPLQNPHDRFWTPQTSCAAGTATCTTGACGGGATGCAGGACGGCATGTCCTACCTGCTCGGT KKFPAPPPGIIAGGPNAGAAACGGACTTGATCAATCGTACGTTACTGGGTACGGGGAGAGGC SRFEDPTITAAVKKDTCACTTCAGAACCCCCACGACCGCTTTTGGACCCCTCAAACTTCGAA PPQKCYIDHTDSWSTNGAAATTCCCGGCCCCACCCCCTGGTATTATCGCAGGCGGGCCGAAT EITINWNAPFAWVTAYAGCCGGTTTGAAGATCCAACGATCACTGCAGCGGTTAAGAAGGATA LDEIDLITPPGGVDPECACCCCCGCAGAAGTGCTATATTGACCACACCGATTCCTGGTCTAC EPEVIYGDCNGDGKVNTAACGAGATCACGATTAATTGGAACGCCCCCTTCGCGTGGGTCACA STDAVALKRYILRSGIGCGTATCTGGACGAAATTGACTTGATTACCCCACCCGGCGGAGTGG SINTDNADVNADGRVNACCCTGAAGAGCCGGAAGTTATCTACGGTGATTGTAACGGCGACGG STDLAILKRYILKEIDAAAGGTTAATTCGACCGATGCTGTGGCCCTTAAAAGGTATATCCTC VLPHK* (SEQ IDCGCAGCGGTATCTCGATCAACACGGACAACGCGGACGTTAATGCAG NO: 97)ATGGTCGCGTGAATAGCACTGACCTCGCTATTTTGAAGCGCTATATTTTGAAGGAGATCGATGTTCTTCCTCACAAGTGA (SEQ ID NO: 169) 2070 PR1a:O68438MGFVLFSQLPSFLLVS ATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCTTLLLFLVISHSCRAQN CCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCCALEDKSPKLPDYKNDLL GAACCTGGAGGACAAGTCTCCCAAACTGCCTGATTATAAGAACGACYERTFDEGLCFPWHTC CTTCTGTACGAACGCACATTCGACGAGGGGCTCTGCTTCCCGTGGCEDSGGKCDFAVVDVPG ACACGTGCGAAGATTCAGGAGGGAAATGCGATTTTGCCGTGGTCGAEPGNKAFRLTVIDKGQ CGTTCCAGGCGAGCCTGGGAACAAGGCGTTCAGGCTCACTGTTATCNKWSVQMRHRGITLEQ GATAAGGGTCAGAACAAGTGGTCGGTCCAAATGAGACACCGGGGTAGHTYTVRFTIWSDKSC TCACGTTGGAGCAGGGGCACACATACACCGTTCGGTTTACTATCTGRVYAKIGQMGEPYTEY GAGCGACAAGAGCTGCCGCGTGTATGCCAAAATCGGCCAAATGGGTWNNNWNPFNLTPGQKL GAACCCTACACGGAGTACTGGAACAATAACTGGAATCCGTTCAACCTVEQNFTMNYPTDDTC TCACTCCGGGGCAGAAATTGACGGTGGAACAGAACTTTACTATGAAEFTFHLGGELAAGTPY TTATCCCACGGACGACACGTGTGAGTTTACCTTCCACTTGGGAGGGYVYLDDVSLYDPRFVK GAACTGGCAGCCGGGACCCCTTACTACGTGTACCTCGACGACGTTTPVEYVLPQPDVRVNQV CTCTTTACGATCCCCGCTTTGTCAAGCCAGTGGAATACGTCCTGCCGYLPFAKKYATVVSSS TCAACCGGATGTCAGGGTTAATCAAGTTGGATACCTCCCTTTTGCTTSPLKWQLLNSANQVV AAGAAATATGCTACTGTCGTGTCATCGAGCACGTCCCCATTGAAGTLEGNTIPKGLDKDSQD GGCAACTTCTGAATAGTGCAAACCAAGTTGTCTTGGAGGGCAATACYVHWIDFSNFKTEGKG AATCCCCAAGGGACTGGACAAAGATTCACAAGACTACGTTCATTGGYYFKLPTVNSDTNYSH ATCGATTTCTCGAACTTTAAGACCGAAGGCAAGGGGTACTATTTCAPFDISADIYSKMKFDA AGTTGCCCACTGTGAACTCCGATACTAACTACTCCCACCCGTTTGALAFFYHKRSGIPIEMP TATTTCTGCAGATATCTATTCAAAGATGAAGTTCGACGCGCTCGCTYAGGEQWTRPAGHIGV TTCTTTTACCATAAAAGGTCGGGAATACCAATCGAGATGCCCTACGAPNKGDTNVPTWPQDD CCGGGGGAGAGCAGTGGACAAGGCCCGCAGGGCACATTGGTGTCGCEYAGRPQKYYTKDVTG GCCGAACAAGGGCGACACGAATGTGCCAACTTGGCCCCAGGATGACGWYDAGDHGKYVVNGG GAATATGCTGGACGCCCCCAGAAATACTATACGAAAGACGTGACCGIAVWTLMNMYERAKIR GCGGGTGGTACGATGCCGGTGACCACGGCAAGTACGTCGTGAACGGGIANQGAYKDGGMNIP GGGTATCGCAGTTTGGACCCTTATGAATATGTACGAGAGAGCAAAGERNNGYPDILDEARWE ATTAGAGGAATCGCTAACCAGGGTGCCTACAAAGATGGAGGAATGAIEFFKKMQVTEKEDPS ATATCCCGGAAAGGAATAACGGCTATCCTGATATTCTGGACGAGGCIAGMVHHKIHDFRWTA CAGATGGGAGATCGAATTTTTTAAGAAGATGCAAGTCACTGAGAAALGMLPHEDPQPRYLRP GAAGATCCGTCGATTGCAGGTATGGTGCACCACAAGATCCACGATTVSTAATLNFAATLAQS TCAGGTGGACGGCGCTCGGAATGTTGCCTCACGAGGACCCCCAGCCARLWKDYDPTFAADCL ACGCTACCTTCGGCCCGTCAGCACAGCGGCAACCCTGAATTTCGCAEKAEIAWQAALKHPDI GCGACCCTCGCTCAGTCTGCCAGATTGTGGAAGGATTACGACCCGAYAEYTPGSGGPGGGPY CTTTTGCAGCGGACTGCCTTGAGAAAGCTGAAATTGCCTGGCAAGCNDDYVGDEFYWAACEL AGCACTCAAACACCCGGACATCTACGCTGAGTACACGCCAGGAAGCYVTTGKDEYKNYLMNS GGTGGGCCGGGTGGAGGTCCTTATAATGACGATTATGTCGGGGACGPHYLEMPAKMGENGGA AGTTCTACTGGGCCGCTTGTGAACTCTATGTGACAACCGGTAAGGANGEDNGLWGCFTWGTT TGAGTACAAGAATTACTTGATGAATAGTCCGCACTATCTGGAAATGQGLGTITLALVENGLP CCAGCGAAGATGGGCGAGAACGGAGGGGCTAACGGCGAGGACAACGSADIQKARNNIAKAAD GTCTCTGGGGCTGCTTTACTTGGGGAACGACACAGGGGTTGGGTACKWLENIEEQGYRLPIK AATTACCCTTGCCCTCGTTGAAAACGGCCTCCCTTCGGCGGATATTQAEDERGGYPWGSNSF CAAAAGGCCCGCAACAATATCGCTAAAGCCGCAGATAAGTGGCTTGILNQMIVMGYAYDFTG AGAATATTGAAGAACAAGGTTACCGCCTGCCTATCAAACAAGCGGANSKYLDGMQDGMSYLL GGATGAACGGGGCGGATACCCGTGGGGTAGTAATTCTTTCATTCTCGRNGLDQSYVTGYGER AACCAGATGATCGTCATGGGCTACGCTTACGACTTCACGGGAAACAPLQNPHDRFWTPQTSK GCAAGTATCTTGACGGGATGCAGGACGGCATGTCCTACCTGCTCGGKFPAPPPGIIAGGPNS TAGAAACGGACTTGATCAATCGTACGTTACTGGGTACGGGGAGAGGRFEDPTITAAVKKDTP CCACTTCAGAACCCCCACGACCGCTTTTGGACCCCTCAAACTTCGAPQKCYIDHTDSWSTNE AGAAATTCCCGGCCCCACCCCCTGGTATTATCGCAGGCGGGCCGAAITINWNAPFAWVTAYL TAGCCGGTTTGAAGATCCAACGATCACTGCAGCGGTTAAGAAGGATDEIDLITPPGGVDPEE ACACCCCCGCAGAAGTGCTATATTGACCACACCGATTCCTGGTCTAPEVIYGDCNGDGKVNS CTAACGAGATCACGATTAATTGGAACGCCCCCTTCGCGTGGGTCACTDAVALKRYILRSGIS AGCGTATCTGGACGAAATTGACTTGATTACCCCACCCGGCGGAGTGINTDNADVNADGRVNS GACCCTGAAGAGCCGGAAGTTATCTACGGTGATTGTAACGGCGACGTDLAILKRYILKEIDV GAAAGGTTAATTCGACCGATGCTGTGGCCCTTAAAAGGTATATCCT LPHK*(SEQ ID CCGCAGCGGTATCTCGATCAACACGGACAACGCGGACGTTAATGCA NO: 98)GATGGTCGCGTGAATAGCACTGACCTCGCTATTTTGAAGCGCTATATTTTGAAGGAGATCGATGTTCTTCCTCACAAGTGA (SEQ ID NO: 170) 2071PR1a:O68438:SEKDEL MGFVLFSQLPSFLLVSATGGGCTTCGTGCTCTTCTCCCAGCTGCCTTCCTTCCTTCTTGTCT TLLLFLVISHSCRAQNCCACCCTGCTCTTGTTCCTCGTGATCTCCCACTCCTGCCGCGCCCA LEDKSPKLPDYKNDLLGAACCTGGAGGACAAGTCTCCCAAACTGCCTGATTATAAGAACGAC YERTFDEGLCFPWHTCCTTCTGTACGAACGCACATTCGACGAGGGGCTCTGCTTCCCGTGGC EDSGGKCDFAVVDVPGACACGTGCGAAGATTCAGGAGGGAAATGCGATTTTGCCGTGGTCGA EPGNKAFRLTVIDKGQCGTTCCAGGCGAGCCTGGGAACAAGGCGTTCAGGCTCACTGTTATC NKWSVQMRHRGITLEQGATAAGGGTCAGAACAAGTGGTCGGTCCAAATGAGACACCGGGGTA GHTYTVRFTIWSDKSCTCACGTTGGAGCAGGGGCACACATACACCGTTCGGTTTACTATCTG RVYAKIGQMGEPYTEYGAGCGACAAGAGCTGCCGCGTGTATGCCAAAATCGGCCAAATGGGT WNNNWNPFNLTPGQKLGAACCCTACACGGAGTACTGGAACAATAACTGGAATCCGTTCAACC TVEQNFTMNYPTDDTCTCACTCCGGGGCAGAAATTGACGGTGGAACAGAACTTTACTATGAA EFTFHLGGELAAGTPYTTATCCCACGGACGACACGTGTGAGTTTACCTTCCACTTGGGAGGG YVYLDDVSLYDPRFVKGAACTGGCAGCCGGGACCCCTTACTACGTGTACCTCGACGACGTTT PVEYVLPQPDVRVNQVCTCTTTACGATCCCCGCTTTGTCAAGCCAGTGGAATACGTCCTGCC GYLPFAKKYATVVSSSTCAACCGGATGTCAGGGTTAATCAAGTTGGATACCTCCCTTTTGCT TSPLKWQLLNSANQVVAAGAAATATGCTACTGTCGTGTCATCGAGCACGTCCCCATTGAAGT LEGNTIPKGLDKDSQDGGCAACTTCTGAATAGTGCAAACCAAGTTGTCTTGGAGGGCAATAC YVHWIDFSNFKTEGKGAATCCCCAAGGGACTGGACAAAGATTCACAAGACTACGTTCATTGG YYFKLPTVNSDTNYSHATCGATTTCTCGAACTTTAAGACCGAAGGCAAGGGGTACTATTTCA PFDISADIYSKMKFDAAGTTGCCCACTGTGAACTCCGATACTAACTACTCCCACCCGTTTGA LAFFYHKRSGIPIEMPTATTTCTGCAGATATCTATTCAAAGATGAAGTTCGACGCGCTCGCT YAGGEQWTRPAGHIGVTTCTTTTACCATAAAAGGTCGGGAATACCAATCGAGATGCCCTACG APNKGDTNVPTWPQDDCCGGGGGAGAGCAGTGGACAAGGCCCGCAGGGCACATTGGTGTCGC EYAGRPQKYYTKDVTGGCCGAACAAGGGCGACACGAATGTGCCAACTTGGCCCCAGGATGAC GWYDAGDHGKYVVNGGGAATATGCTGGACGCCCCCAGAAATACTATACGAAAGACGTGACCG IAVWTLMNMYERAKIRGCGGGTGGTACGATGCCGGTGACCACGGCAAGTACGTCGTGAACGG GIANQGAYKDGGMNIPGGGTATCGCAGTTTGGACCCTTATGAATATGTACGAGAGAGCAAAG ERNNGYPDILDEARWEATTAGAGGAATCGCTAACCAGGGTGCCTACAAAGATGGAGGAATGA IEFFKKMQVTEKEDPSATATCCCGGAAAGGAATAACGGCTATCCTGATATTCTGGACGAGGC IAGMVHHKIHDFRWTACAGATGGGAGATCGAATTTTTTAAGAAGATGCAAGTCACTGAGAAA LGMLPHEDPQPRYLRPGAAGATCCGTCGATTGCAGGTATGGTGCACCACAAGATCCACGATT VSTAATLNFAATLAQSTCAGGTGGACGGCGCTCGGAATGTTGCCTCACGAGGACCCCCAGCC ARLWKDYDPTFAADCLACGCTACCTTCGGCCCGTCAGCACAGCGGCAACCCTGAATTTCGCA EKAEIAWQAALKHPDIGCGACCCTCGCTCAGTCTGCCAGATTGTGGAAGGATTACGACCCGA YAEYTPGSGGPGGGPYCTTTTGCAGCGGACTGCCTTGAGAAAGCTGAAATTGCCTGGCAAGC NDDYVGDEFYWAACELAGCACTCAAACACCCGGACATCTACGCTGAGTACACGCCAGGAAGC YVTTGKDEYKNYLMNSGGTGGGCCGGGTGGAGGTCCTTATAATGACGATTATGTCGGGGACG PHYLEMPAKMGENGGAAGTTCTACTGGGCCGCTTGTGAACTCTATGTGACAACCGGTAAGGA NGEDNGLWGCFTWGTTTGAGTACAAGAATTACTTGATGAATAGTCCGCACTATCTGGAAATG QGLGTITLALVENGLPCCAGCGAAGATGGGCGAGAACGGAGGGGCTAACGGCGAGGACAACG SADIQKARNNIAKAADGTCTCTGGGGCTGCTTTACTTGGGGAACGACACAGGGGTTGGGTAC KWLENIEEQGYRLPIKAATTACCCTTGCCCTCGTTGAAAACGGCCTCCCTTCGGCGGATATT QAEDERGGYPWGSNSFCAAAAGGCCCGCAACAATATCGCTAAAGCCGCAGATAAGTGGCTTG ILNQMIVMGYAYDFTGAGAATATTGAAGAACAAGGTTACCGCCTGCCTATCAAACAAGCGGA NSKYLDGMQDGMSYLLGGATGAACGGGGCGGATACCCGTGGGGTAGTAATTCTTTCATTCTC GRNGLDQSYVTGYGERAACCAGATGATCGTCATGGGCTACGCTTACGACTTCACGGGAAACA PLQNPHDRFWTPQTSKGCAAGTATCTTGACGGGATGCAGGACGGCATGTCCTACCTGCTCGG KFPAPPPGIIAGGPNSTAGAAACGGACTTGATCAATCGTACGTTACTGGGTACGGGGAGAGG RFEDPTITAAVKKDTPCCACTTCAGAACCCCCACGACCGCTTTTGGACCCCTCAAACTTCGA PQKCYIDHTDSWSTNEAGAAATTCCCGGCCCCACCCCCTGGTATTATCGCAGGCGGGCCGAA ITINWNAPFAWVTAYLTAGCCGGTTTGAAGATCCAACGATCACTGCAGCGGTTAAGAAGGAT DEIDLITPPGGVDPEEACACCCCCGCAGAAGTGCTATATTGACCACACCGATTCCTGGTCTA PEVIYGDCNGDGKVNSCTAACGAGATCACGATTAATTGGAACGCCCCCTTCGCGTGGGTCAC TDAVALKRYILRSGISAGCGTATCTGGACGAAATTGACTTGATTACCCCACCCGGCGGAGTG INTDNADVNADGRVNSGACCCTGAAGAGCCGGAAGTTATCTACGGTGATTGTAACGGCGACG TDLAILKRYILKEIDVGAAAGGTTAATTCGACCGATGCTGTGGCCCTTAAAAGGTATATCCT LPHKSEKDEL*CCGCAGCGGTATCTCGATCAACACGGACAACGCGGACGTTAATGCA (SEQ ID NO: 99)GATGGTCGCGTGAATAGCACTGACCTCGCTATTTTGAAGCGCTATATTTTGAAGGAGATCGATGTTCTTCCTCACAAGAGCGAGAAGGACGA GCTGTGA (SEQ ID NO: 171)2072 BAASS:O68438 MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQVLEDKSPGCCTGTCGGCCAGCTTGGCCTCCGGGCAAGTCCTGGAGGACAAGTC KLPDYKNDLLYERTFDTCCCAAACTGCCTGATTATAAGAACGACCTTCTGTACGAACGCACA EGLCFPWHTCEDSGGKTTCGACGAGGGGCTCTGCTTCCCGTGGCACACGTGCGAAGATTCAG CDFAVVDVPGEPGNKAGAGGGAAATGCGATTTTGCCGTGGTCGACGTTCCAGGCGAGCCTGG FRLTVIDKGQNKWSVQGAACAAGGCGTTCAGGCTCACTGTTATCGATAAGGGTCAGAACAAG MRHRGITLEQGHTYTVTGGTCGGTCCAAATGAGACACCGGGGTATCACGTTGGAGCAGGGGC RFTIWSDKSCRVYAKIACACATACACCGTTCGGTTTACTATCTGGAGCGACAAGAGCTGCCG GQMGEPYTEYWNNNWNCGTGTATGCCAAAATCGGCCAAATGGGTGAACCCTACACGGAGTAC PFNLTPGQKLTVEQNFTGGAACAATAACTGGAATCCGTTCAACCTCACTCCGGGGCAGAAAT TMNYPTDDTCEFTFHLTGACGGTGGAACAGAACTTTACTATGAATTATCCCACGGACGACAC GGELAAGTPYYVYLDDGTGTGAGTTTACCTTCCACTTGGGAGGGGAACTGGCAGCCGGGACC VSLYDPRFVKPVEYVLCCTTACTACGTGTACCTCGACGACGTTTCTCTTTACGATCCCCGCT PQPDVRVNQVGYLPFATTGTCAAGCCAGTGGAATACGTCCTGCCTCAACCGGATGTCAGGGT KKYATVVSSSTSPLKWTAATCAAGTTGGATACCTCCCTTTTGCTAAGAAATATGCTACTGTC QLLNSANQVVLEGNTIGTGTCATCGAGCACGTCCCCATTGAAGTGGCAACTTCTGAATAGTG PKGLDKDSQDYVHWIDCAAACCAAGTTGTCTTGGAGGGCAATACAATCCCCAAGGGACTGGA FSNFKTEGKGYYFKLPCAAAGATTCACAAGACTACGTTCATTGGATCGATTTCTCGAACTTT TVNSDTNYSHPFDISAAAGACCGAAGGCAAGGGGTACTATTTCAAGTTGCCCACTGTGAACT DIYSKMKFDALAFFYHCCGATACTAACTACTCCCACCCGTTTGATATTTCTGCAGATATCTA KRSGIPIEMPYAGGEQTTCAAAGATGAAGTTCGACGCGCTCGCTTTCTTTTACCATAAAAGG WTRPAGHIGVAPNKGDTCGGGAATACCAATCGAGATGCCCTACGCCGGGGGAGAGCAGTGGA TNVPTWPQDDEYAGRPCAAGGCCCGCAGGGCACATTGGTGTCGCGCCGAACAAGGGCGACAC QKYYTKDVTGGWYDAGGAATGTGCCAACTTGGCCCCAGGATGACGAATATGCTGGACGCCCC DHGKYVVNGGIAVWTLCAGAAATACTATACGAAAGACGTGACCGGCGGGTGGTACGATGCCG MNMYERAKIRGIANQGGTGACCACGGCAAGTACGTCGTGAACGGGGGTATCGCAGTTTGGAC AYKDGGMNIPERNNGYCCTTATGAATATGTACGAGAGAGCAAAGATTAGAGGAATCGCTAAC PDILDEARWEIEFFKKCAGGGTGCCTACAAAGATGGAGGAATGAATATCCCGGAAAGGAATA MQVTEKEDPSIAGMVHACGGCTATCCTGATATTCTGGACGAGGCCAGATGGGAGATCGAATT HKIHDFRWTALGMLPHTTTTAAGAAGATGCAAGTCACTGAGAAAGAAGATCCGTCGATTGCA EDPQPRYLRPVSTAATGGTATGGTGCACCACAAGATCCACGATTTCAGGTGGACGGCGCTCG LNFAATLAQSARLWKDGAATGTTGCCTCACGAGGACCCCCAGCCACGCTACCTTCGGCCCGT YDPTFAADCLEKAEIACAGCACAGCGGCAACCCTGAATTTCGCAGCGACCCTCGCTCAGTCT WQAALKHPDIYAEYTPGCCAGATTGTGGAAGGATTACGACCCGACTTTTGCAGCGGACTGCC GSGGPGGGPYNDDYVGTTGAGAAAGCTGAAATTGCCTGGCAAGCAGCACTCAAACACCCGGA DEFYWAACELYVTTGKCATCTACGCTGAGTACACGCCAGGAAGCGGTGGGCCGGGTGGAGGT DEYKNYLMNSPHYLEMCCTTATAATGACGATTATGTCGGGGACGAGTTCTACTGGGCCGCTT PAKMGENGGANGEDNGGTGAACTCTATGTGACAACCGGTAAGGATGAGTACAAGAATTACTT LWGCFTWGTTQGLGTIGATGAATAGTCCGCACTATCTGGAAATGCCAGCGAAGATGGGCGAG TLALVENGLPSADIQKAACGGAGGGGCTAACGGCGAGGACAACGGTCTCTGGGGCTGCTTTA ARNNIAKAADKWLENICTTGGGGAACGACACAGGGGTTGGGTACAATTACCCTTGCCCTCGT EEQGYRLPIKQAEDERTGAAAACGGCCTCCCTTCGGCGGATATTCAAAAGGCCCGCAACAAT GGYPWGSNSFILNQMIATCGCTAAAGCCGCAGATAAGTGGCTTGAGAATATTGAAGAACAAG VMGYAYDFTGNSKYLDGTTACCGCCTGCCTATCAAACAAGCGGAGGATGAACGGGGCGGATA GMQDGMSYLLGRNGLDCCCGTGGGGTAGTAATTCTTTCATTCTCAACCAGATGATCGTCATG QSYVTGYGERPLQNPHGGCTACGCTTACGACTTCACGGGAAACAGCAAGTATCTTGACGGGA DRFWTPQTSKKFPAPPTGCAGGACGGCATGTCCTACCTGCTCGGTAGAAACGGACTTGATCA PGIIAGGPNSRFEDPTATCGTACGTTACTGGGTACGGGGAGAGGCCACTTCAGAACCCCCAC ITAAVKKDTPPQKCYIGACCGCTTTTGGACCCCTCAAACTTCGAAGAAATTCCCGGCCCCAC DHTDSWSTNEITINWNCCCCTGGTATTATCGCAGGCGGGCCGAATAGCCGGTTTGAAGATCC APFAWVTAYLDEIDLIAACGATCACTGCAGCGGTTAAGAAGGATACACCCCCGCAGAAGTGC TPPGGVDPEEPEVIYGTATATTGACCACACCGATTCCTGGTCTACTAACGAGATCACGATTA DCNGDGKVNSTDAVALATTGGAACGCCCCCTTCGCGTGGGTCACAGCGTATCTGGACGAAAT KRYILRSGISINTDNATGACTTGATTACCCCACCCGGCGGAGTGGACCCTGAAGAGCCGGAA DVNADGRVNSTDLAILGTTATCTACGGTGATTGTAACGGCGACGGAAAGGTTAATTCGACCG KRYILKEIDVLPHK*ATGCTGTGGCCCTTAAAAGGTATATCCTCCGCAGCGGTATCTCGAT (SEQ ID NO: 100)CAACACGGACAACGCGGACGTTAATGCAGATGGTCGCGTGAATAGCACTGACCTCGCTATTTTGAAGCGCTATATTTTGAAGGAGATCGATG TTCTTCCTCACAAGTGA (SEQ IDNO: 172) 2073 BAASS:O68438:SEKDEL MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG LSASLASGQVLEDKSPGCCTGTCGGCCAGCTTGGCCTCCGGGCAAGTCCTGGAGGACAAGTC KLPDYKNDLLYERTFDTCCCAAACTGCCTGATTATAAGAACGACCTTCTGTACGAACGCACA EGLCFPWHTCEDSGGKTTCGACGAGGGGCTCTGCTTCCCGTGGCACACGTGCGAAGATTCAG CDFAVVDVPGEPGNKAGAGGGAAATGCGATTTTGCCGTGGTCGACGTTCCAGGCGAGCCTGG FRLTVIDKGQNKWSVQGAACAAGGCGTTCAGGCTCACTGTTATCGATAAGGGTCAGAACAAG MRHRGITLEQGHTYTVTGGTCGGTCCAAATGAGACACCGGGGTATCACGTTGGAGCAGGGGC RFTIWSDKSCRVYAKIACACATACACCGTTCGGTTTACTATCTGGAGCGACAAGAGCTGCCG GQMGEPYTEYWNNNWNCGTGTATGCCAAAATCGGCCAAATGGGTGAACCCTACACGGAGTAC PFNLTPGQKLTVEQNFTGGAACAATAACTGGAATCCGTTCAACCTCACTCCGGGGCAGAAAT TMNYPTDDTCEFTFHLTGACGGTGGAACAGAACTTTACTATGAATTATCCCACGGACGACAC GGELAAGTPYYVYLDDGTGTGAGTTTACCTTCCACTTGGGAGGGGAACTGGCAGCCGGGACC VSLYDPRFVKPVEYVLCCTTACTACGTGTACCTCGACGACGTTTCTCTTTACGATCCCCGCT PQPDVRVNQVGYLPFATTGTCAAGCCAGTGGAATACGTCCTGCCTCAACCGGATGTCAGGGT KKYATVVSSSTSPLKWTAATCAAGTTGGATACCTCCCTTTTGCTAAGAAATATGCTACTGTC QLLNSANQVVLEGNTIGTGTCATCGAGCACGTCCCCATTGAAGTGGCAACTTCTGAATAGTG PKGLDKDSQDYVHWIDCAAACCAAGTTGTCTTGGAGGGCAATACAATCCCCAAGGGACTGGA FSNFKTEGKGYYFKLPCAAAGATTCACAAGACTACGTTCATTGGATCGATTTCTCGAACTTT TVNSDTNYSHPFDISAAAGACCGAAGGCAAGGGGTACTATTTCAAGTTGCCCACTGTGAACT DIYSKMKFDALAFFYHCCGATACTAACTACTCCCACCCGTTTGATATTTCTGCAGATATCTA KRSGIPIEMPYAGGEQTTCAAAGATGAAGTTCGACGCGCTCGCTTTCTTTTACCATAAAAGG WTRPAGHIGVAPNKGDTCGGGAATACCAATCGAGATGCCCTACGCCGGGGGAGAGCAGTGGA TNVPTWPQDDEYAGRPCAAGGCCCGCAGGGCACATTGGTGTCGCGCCGAACAAGGGCGACAC QKYYTKDVTGGWYDAGGAATGTGCCAACTTGGCCCCAGGATGACGAATATGCTGGACGCCCC DHGKYVVNGGIAVWTLCAGAAATACTATACGAAAGACGTGACCGGCGGGTGGTACGATGCCG MNMYERAKIRGIANQGGTGACCACGGCAAGTACGTCGTGAACGGGGGTATCGCAGTTTGGAC AYKDGGMNIPERNNGYCCTTATGAATATGTACGAGAGAGCAAAGATTAGAGGAATCGCTAAC PDILDEARWEIEFFKKCAGGGTGCCTACAAAGATGGAGGAATGAATATCCCGGAAAGGAATA MQVTEKEDPSIAGMVHACGGCTATCCTGATATTCTGGACGAGGCCAGATGGGAGATCGAATT HKIHDFRWTALGMLPHTTTTAAGAAGATGCAAGTCACTGAGAAAGAAGATCCGTCGATTGCA EDPQPRYLRPVSTAATGGTATGGTGCACCACAAGATCCACGATTTCAGGTGGACGGCGCTCG LNFAATLAQSARLWKDGAATGTTGCCTCACGAGGACCCCCAGCCACGCTACCTTCGGCCCGT YDPTFAADCLEKAEIACAGCACAGCGGCAACCCTGAATTTCGCAGCGACCCTCGCTCAGTCT WQAALKHPDIYAEYTPGCCAGATTGTGGAAGGATTACGACCCGACTTTTGCAGCGGACTGCC GSGGPGGGPYNDDYVGTTGAGAAAGCTGAAATTGCCTGGCAAGCAGCACTCAAACACCCGGA DEFYWAACELYVTTGKCATCTACGCTGAGTACACGCCAGGAAGCGGTGGGCCGGGTGGAGGT DEYKNYLMNSPHYLEMCCTTATAATGACGATTATGTCGGGGACGAGTTCTACTGGGCCGCTT PAKMGENGGANGEDNGGTGAACTCTATGTGACAACCGGTAAGGATGAGTACAAGAATTACTT LWGCFTWGTTQGLGTIGATGAATAGTCCGCACTATCTGGAAATGCCAGCGAAGATGGGCGAG TLALVENGLPSADIQKAACGGAGGGGCTAACGGCGAGGACAACGGTCTCTGGGGCTGCTTTA ARNNIAKAADKWLENICTTGGGGAACGACACAGGGGTTGGGTACAATTACCCTTGCCCTCGT EEQGYRLPIKQAEDERTGAAAACGGCCTCCCTTCGGCGGATATTCAAAAGGCCCGCAACAAT GGYPWGSNSFILNQMIATCGCTAAAGCCGCAGATAAGTGGCTTGAGAATATTGAAGAACAAG VMGYAYDFTGNSKYLDGTTACCGCCTGCCTATCAAACAAGCGGAGGATGAACGGGGCGGATA GMQDGMSYLLGRNGLDCCCGTGGGGTAGTAATTCTTTCATTCTCAACCAGATGATCGTCATG QSYVTGYGERPLQNPHGGCTACGCTTACGACTTCACGGGAAACAGCAAGTATCTTGACGGGA DRFWTPQTSKKFPAPPTGCAGGACGGCATGTCCTACCTGCTCGGTAGAAACGGACTTGATCA PGIIAGGPNSRFEDPTATCGTACGTTACTGGGTACGGGGAGAGGCCACTTCAGAACCCCCAC ITAAVKKDTPPQKCYIGACCGCTTTTGGACCCCTCAAACTTCGAAGAAATTCCCGGCCCCAC DHTDSWSTNEITINWNCCCCTGGTATTATCGCAGGCGGGCCGAATAGCCGGTTTGAAGATCC APFAWVTAYLDEIDLIAACGATCACTGCAGCGGTTAAGAAGGATACACCCCCGCAGAAGTGC TPPGGVDPEEPEVIYGTATATTGACCACACCGATTCCTGGTCTACTAACGAGATCACGATTA DCNGDGKVNSTDAVALATTGGAACGCCCCCTTCGCGTGGGTCACAGCGTATCTGGACGAAAT KRYILRSGISINTDNATGACTTGATTACCCCACCCGGCGGAGTGGACCCTGAAGAGCCGGAA DVNADGRVNSTDLAILGTTATCTACGGTGATTGTAACGGCGACGGAAAGGTTAATTCGACCG KRYILKEIDVLPHKSEATGCTGTGGCCCTTAAAAGGTATATCCTCCGCAGCGGTATCTCGAT KDEL* (SEQ IDCAACACGGACAACGCGGACGTTAATGCAGATGGTCGCGTGAATAGC NO: 101)ACTGACCTCGCTATTTTGAAGCGCTATATTTTGAAGGAGATCGATGTTCTTCCTCACAAGAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 173) 2074 HvAleSP:O68438MAHARVLLLALAVLAT ATGGCCCACGCCCGCGTCCTCCTCCTGGCGCTCGCCGTCCTGGCCAAAVAVASSSSFADSNP CCGCCGCCGTCGCCGTCGCCTCCTCCTCCTCCTTCGCCGACTCCAAIRPVTDRAASTLEDKS CCCGATCCGCCCGGTGACCGACCGCGCCGCCTCCACCCTGGAGGACPKLPDYKNDLLYERTF AAGTCTCCCAAACTGCCTGATTATAAGAACGACCTTCTGTACGAACDEGLCFPWHTCEDSGG GCACATTCGACGAGGGGCTCTGCTTCCCGTGGCACACGTGCGAAGAKCDFAVVDVPGEPGNK TTCAGGAGGGAAATGCGATTTTGCCGTGGTCGACGTTCCAGGCGAGAFRLTVIDKGQNKWSV CCTGGGAACAAGGCGTTCAGGCTCACTGTTATCGATAAGGGTCAGAQMRHRGITLEQGHTYT ACAAGTGGTCGGTCCAAATGAGACACCGGGGTATCACGTTGGAGCAVRFTIWSDKSCRVYAK GGGGCACACATACACCGTTCGGTTTACTATCTGGAGCGACAAGAGCIGQMGEPYTEYWNNNW TGCCGCGTGTATGCCAAAATCGGCCAAATGGGTGAACCCTACACGGNPFNLTPGQKLTVEQN AGTACTGGAACAATAACTGGAATCCGTTCAACCTCACTCCGGGGCAFTMNYPTDDTCEFTFH GAAATTGACGGTGGAACAGAACTTTACTATGAATTATCCCACGGACLGGELAAGTPYYVYLD GACACGTGTGAGTTTACCTTCCACTTGGGAGGGGAACTGGCAGCCGDVSLYDPRFVKPVEYV GGACCCCTTACTACGTGTACCTCGACGACGTTTCTCTTTACGATCCLPQPDVRVNQVGYLPF CCGCTTTGTCAAGCCAGTGGAATACGTCCTGCCTCAACCGGATGTCAKKYATVVSSSTSPLK AGGGTTAATCAAGTTGGATACCTCCCTTTTGCTAAGAAATATGCTAWQLLNSANQVVLEGNT CTGTCGTGTCATCGAGCACGTCCCCATTGAAGTGGCAACTTCTGAAIPKGLDKDSQDYVHWI TAGTGCAAACCAAGTTGTCTTGGAGGGCAATACAATCCCCAAGGGADFSNFKTEGKGYYFKL CTGGACAAAGATTCACAAGACTACGTTCATTGGATCGATTTCTCGAPTVNSDTNYSHPFDIS ACTTTAAGACCGAAGGCAAGGGGTACTATTTCAAGTTGCCCACTGTADIYSKMKFDALAFFY GAACTCCGATACTAACTACTCCCACCCGTTTGATATTTCTGCAGATHKRSGIPIEMPYAGGE ATCTATTCAAAGATGAAGTTCGACGCGCTCGCTTTCTTTTACCATAQWTRPAGHIGVAPNKG AAAGGTCGGGAATACCAATCGAGATGCCCTACGCCGGGGGAGAGCADTNVPTWPQDDEYAGR GTGGACAAGGCCCGCAGGGCACATTGGTGTCGCGCCGAACAAGGGCPQKYYTKDVTGGWYDA GACACGAATGTGCCAACTTGGCCCCAGGATGACGAATATGCTGGACGDHGKYVVNGGIAVWT GCCCCCAGAAATACTATACGAAAGACGTGACCGGCGGGTGGTACGALMNMYERAKIRGIANQ TGCCGGTGACCACGGCAAGTACGTCGTGAACGGGGGTATCGCAGTTGAYKDGGMNIPERNNG TGGACCCTTATGAATATGTACGAGAGAGCAAAGATTAGAGGAATCGYPDILDEARWEIEFFK CTAACCAGGGTGCCTACAAAGATGGAGGAATGAATATCCCGGAAAGKMQVTEKEDPSIAGMV GAATAACGGCTATCCTGATATTCTGGACGAGGCCAGATGGGAGATCHHKIHDFRWTALGMLP GAATTTTTTAAGAAGATGCAAGTCACTGAGAAAGAAGATCCGTCGAHEDPQPRYLRPVSTAA TTGCAGGTATGGTGCACCACAAGATCCACGATTTCAGGTGGACGGCTLNFAATLAQSARLWK GCTCGGAATGTTGCCTCACGAGGACCCCCAGCCACGCTACCTTCGGDYDPTFAADCLEKAEI CCCGTCAGCACAGCGGCAACCCTGAATTTCGCAGCGACCCTCGCTCAWQAALKHPDIYAEYT AGTCTGCCAGATTGTGGAAGGATTACGACCCGACTTTTGCAGCGGAPGSGGPGGGPYNDDYV CTGCCTTGAGAAAGCTGAAATTGCCTGGCAAGCAGCACTCAAACACGDEFYWAACELYVTTG CCGGACATCTACGCTGAGTACACGCCAGGAAGCGGTGGGCCGGGTGKDEYKNYLMNSPHYLE GAGGTCCTTATAATGACGATTATGTCGGGGACGAGTTCTACTGGGCMPAKMGENGGANGEDN CGCTTGTGAACTCTATGTGACAACCGGTAAGGATGAGTACAAGAATGLWGCFTWGTTQGLGT TACTTGATGAATAGTCCGCACTATCTGGAAATGCCAGCGAAGATGGITLALVENGLPSADIQ GCGAGAACGGAGGGGCTAACGGCGAGGACAACGGTCTCTGGGGCTGKARNNIAKAADKWLEN CTTTACTTGGGGAACGACACAGGGGTTGGGTACAATTACCCTTGCCIEEQGYRLPIKQAEDE CTCGTTGAAAACGGCCTCCCTTCGGCGGATATTCAAAAGGCCCGCARGGYPWGSNSFILNQM ACAATATCGCTAAAGCCGCAGATAAGTGGCTTGAGAATATTGAAGAIVMGYAYDFTGNSKYL ACAAGGTTACCGCCTGCCTATCAAACAAGCGGAGGATGAACGGGGCDGMQDGMSYLLGRNGL GGATACCCGTGGGGTAGTAATTCTTTCATTCTCAACCAGATGATCGDQSYVTGYGERPLQNP TCATGGGCTACGCTTACGACTTCACGGGAAACAGCAAGTATCTTGAHDRFWTPQTSKKFPAP CGGGATGCAGGACGGCATGTCCTACCTGCTCGGTAGAAACGGACTTPPGIIAGGPNSRFEDP GATCAATCGTACGTTACTGGGTACGGGGAGAGGCCACTTCAGAACCTITAAVKKDTPPQKCY CCCACGACCGCTTTTGGACCCCTCAAACTTCGAAGAAATTCCCGGCIDHTDSWSTNEITINW CCCACCCCCTGGTATTATCGCAGGCGGGCCGAATAGCCGGTTTGAANAPFAWVTAYLDEIDL GATCCAACGATCACTGCAGCGGTTAAGAAGGATACACCCCCGCAGAITPPGGVDPEEPEVIY AGTGCTATATTGACCACACCGATTCCTGGTCTACTAACGAGATCACGDCNGDGKVNSTDAVA GATTAATTGGAACGCCCCCTTCGCGTGGGTCACAGCGTATCTGGACLKRYILRSGISINTDN GAAATTGACTTGATTACCCCACCCGGCGGAGTGGACCCTGAAGAGCADVNADGRVNSTDLAI CGGAAGTTATCTACGGTGATTGTAACGGCGACGGAAAGGTTAATTCLKRYILKEIDVLPHK* GACCGATGCTGTGGCCCTTAAAAGGTATATCCTCCGCAGCGGTATC (SEQ IDNO: 102) TCGATCAACACGGACAACGCGGACGTTAATGCAGATGGTCGCGTGAATAGCACTGACCTCGCTATTTTGAAGCGCTATATTTTGAAGGAGAT CGATGTTCTTCCTCACAAGTGA(SEQ ID NO: 174) 2075 HvAleSP:O68438: MAHARVLLLALAVLATATGGCCCACGCCCGCGTCCTCCTCCTGGCGCTCGCCGTCCTGGCCA SEKDEL AAVAVASSSSFADSNPCCGCCGCCGTCGCCGTCGCCTCCTCCTCCTCCTTCGCCGACTCCAA IRPVTDRAASTLEDKSCCCGATCCGCCCGGTGACCGACCGCGCCGCCTCCACCCTGGAGGAC PKLPDYKNDLLYERTFAAGTCTCCCAAACTGCCTGATTATAAGAACGACCTTCTGTACGAAC DEGLCFPWHTCEDSGGGCACATTCGACGAGGGGCTCTGCTTCCCGTGGCACACGTGCGAAGA KCDFAVVDVPGEPGNKTTCAGGAGGGAAATGCGATTTTGCCGTGGTCGACGTTCCAGGCGAG AFRLTVIDKGQNKWSVCCTGGGAACAAGGCGTTCAGGCTCACTGTTATCGATAAGGGTCAGA QMRHRGITLEQGHTYTACAAGTGGTCGGTCCAAATGAGACACCGGGGTATCACGTTGGAGCA VRFTIWSDKSCRVYAKGGGGCACACATACACCGTTCGGTTTACTATCTGGAGCGACAAGAGC IGQMGEPYTEYWNNNWTGCCGCGTGTATGCCAAAATCGGCCAAATGGGTGAACCCTACACGG NPFNLTPGQKLTVEQNAGTACTGGAACAATAACTGGAATCCGTTCAACCTCACTCCGGGGCA FTMNYPTDDTCEFTFHGAAATTGACGGTGGAACAGAACTTTACTATGAATTATCCCACGGAC LGGELAAGTPYYVYLDGACACGTGTGAGTTTACCTTCCACTTGGGAGGGGAACTGGCAGCCG DVSLYDPRFVKPVEYVGGACCCCTTACTACGTGTACCTCGACGACGTTTCTCTTTACGATCC LPQPDVRVNQVGYLPFCCGCTTTGTCAAGCCAGTGGAATACGTCCTGCCTCAACCGGATGTC AKKYATVVSSSTSPLKAGGGTTAATCAAGTTGGATACCTCCCTTTTGCTAAGAAATATGCTA WQLLNSANQVVLEGNTCTGTCGTGTCATCGAGCACGTCCCCATTGAAGTGGCAACTTCTGAA IPKGLDKDSQDYVHWITAGTGCAAACCAAGTTGTCTTGGAGGGCAATACAATCCCCAAGGGA DFSNFKTEGKGYYFKLCTGGACAAAGATTCACAAGACTACGTTCATTGGATCGATTTCTCGA PTVNSDTNYSHPFDISACTTTAAGACCGAAGGCAAGGGGTACTATTTCAAGTTGCCCACTGT ADIYSKMKFDALAFFYGAACTCCGATACTAACTACTCCCACCCGTTTGATATTTCTGCAGAT HKRSGIPIEMPYAGGEATCTATTCAAAGATGAAGTTCGACGCGCTCGCTTTCTTTTACCATA QWTRPAGHIGVAPNKGAAAGGTCGGGAATACCAATCGAGATGCCCTACGCCGGGGGAGAGCA DTNVPTWPQDDEYAGRGTGGACAAGGCCCGCAGGGCACATTGGTGTCGCGCCGAACAAGGGC PQKYYTKDVTGGWYDAGACACGAATGTGCCAACTTGGCCCCAGGATGACGAATATGCTGGAC GDHGKYVVNGGIAVWTGCCCCCAGAAATACTATACGAAAGACGTGACCGGCGGGTGGTACGA LMNMYERAKIRGIANQTGCCGGTGACCACGGCAAGTACGTCGTGAACGGGGGTATCGCAGTT GAYKDGGMNIPERNNGTGGACCCTTATGAATATGTACGAGAGAGCAAAGATTAGAGGAATCG YPDILDEARWEIEFFKCTAACCAGGGTGCCTACAAAGATGGAGGAATGAATATCCCGGAAAG KMQVTEKEDPSIAGMVGAATAACGGCTATCCTGATATTCTGGACGAGGCCAGATGGGAGATC HHKIHDFRWTALGMLPGAATTTTTTAAGAAGATGCAAGTCACTGAGAAAGAAGATCCGTCGA HEDPQPRYLRPVSTAATTGCAGGTATGGTGCACCACAAGATCCACGATTTCAGGTGGACGGC TLNFAATLAQSARLWKGCTCGGAATGTTGCCTCACGAGGACCCCCAGCCACGCTACCTTCGG DYDPTFAADCLEKAEICCCGTCAGCACAGCGGCAACCCTGAATTTCGCAGCGACCCTCGCTC AWQAALKHPDIYAEYTAGTCTGCCAGATTGTGGAAGGATTACGACCCGACTTTTGCAGCGGA PGSGGPGGGPYNDDYVCTGCCTTGAGAAAGCTGAAATTGCCTGGCAAGCAGCACTCAAACAC GDEFYWAACELYVTTGCCGGACATCTACGCTGAGTACACGCCAGGAAGCGGTGGGCCGGGTG KDEYKNYLMNSPHYLEGAGGTCCTTATAATGACGATTATGTCGGGGACGAGTTCTACTGGGC MPAKMGENGGANGEDNCGCTTGTGAACTCTATGTGACAACCGGTAAGGATGAGTACAAGAAT GLWGCFTWGTTQGLGTTACTTGATGAATAGTCCGCACTATCTGGAAATGCCAGCGAAGATGG ITLALVENGLPSADIQGCGAGAACGGAGGGGCTAACGGCGAGGACAACGGTCTCTGGGGCTG KARNNIAKAADKWLENCTTTACTTGGGGAACGACACAGGGGTTGGGTACAATTACCCTTGCC IEEQGYRLPIKQAEDECTCGTTGAAAACGGCCTCCCTTCGGCGGATATTCAAAAGGCCCGCA RGGYPWGSNSFILNQMACAATATCGCTAAAGCCGCAGATAAGTGGCTTGAGAATATTGAAGA IVMGYAYDFTGNSKYLACAAGGTTACCGCCTGCCTATCAAACAAGCGGAGGATGAACGGGGC DGMQDGMSYLLGRNGLGGATACCCGTGGGGTAGTAATTCTTTCATTCTCAACCAGATGATCG DQSYVTGYGERPLQNPTCATGGGCTACGCTTACGACTTCACGGGAAACAGCAAGTATCTTGA HDRFWTPQTSKKFPAPCGGGATGCAGGACGGCATGTCCTACCTGCTCGGTAGAAACGGACTT PPGIIAGGPNSRFEDPGATCAATCGTACGTTACTGGGTACGGGGAGAGGCCACTTCAGAACC TITAAVKKDTPPQKCYCCCACGACCGCTTTTGGACCCCTCAAACTTCGAAGAAATTCCCGGC IDHTDSWSTNEITINWCCCACCCCCTGGTATTATCGCAGGCGGGCCGAATAGCCGGTTTGAA NAPFAWVTAYLDEIDLGATCCAACGATCACTGCAGCGGTTAAGAAGGATACACCCCCGCAGA ITPPGGVDPEEPEVIYAGTGCTATATTGACCACACCGATTCCTGGTCTACTAACGAGATCAC GDCNGDGKVNSTDAVAGATTAATTGGAACGCCCCCTTCGCGTGGGTCACAGCGTATCTGGAC LKRYILRSGISINTDNGAAATTGACTTGATTACCCCACCCGGCGGAGTGGACCCTGAAGAGC ADVNADGRVNSTDLAICGGAAGTTATCTACGGTGATTGTAACGGCGACGGAAAGGTTAATTC LKRYILKEIDVLPHKSGACCGATGCTGTGGCCCTTAAAAGGTATATCCTCCGCAGCGGTATC EKDEL* (SEQ IDTCGATCAACACGGACAACGCGGACGTTAATGCAGATGGTCGCGTGA NO: 103)ATAGCACTGACCTCGCTATTTTGAAGCGCTATATTTTGAAGGAGATCGATGTTCTTCCTCACAAGAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 175) 2076P77853S158-2 MQTSITLTSNASGTFDATGCAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTG GYYYELWKDTGNTTMTACGGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAAT VYTQGRFSCQWSNINNGACGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCGAACATC ALFRTGKKYNQNWQSLAATAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTGGC GTIRITYSATYNPNGNAGTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAACCC SYLCIYGWSTNPLVEFAAACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACCCA YIVESWGNWRPPGATSTTGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACCGC LGQVTIDGGTYDIYRTCTGGTGCCACGTCCCTGGGCCAAGTGACAATCGATGGCGGGACCTA TRVNQPCLAEGSLVLDCGACATCTATAGGACGACACGCGTCAACCAGCCTTGCCTGGCCGAG AATGQRVPIEKVRPGMGGCTCGCTCGTCTTGGACGCGGCTACCGGGCAGAGGGTCCCTATCG EVFSLGPDYRLYRVPVAAAAGGTGCGTCCGGGGATGGAAGTTTTCTCCTTGGGACCTGATTA LEVLESGVREVVRLRTCAGACTGTATCGGGTGCCCGTTTTGGAGGTCCTTGAGAGCGGGGTT RSGRTLVLTPDHPLLTAGGGAAGTTGTGCGCCTCAGAACTCGGTCAGGGAGAACGCTGGTGT PEGWKPLCDLPLGTPITGACACCAGATCACCCGCTTTTGACCCCCGAAGGTTGGAAACCTCT AVPAELPVAGHLAPPETTGTGACCTCCCGCTTGGAACTCCAATTGCAGTCCCCGCAGAACTG ERVTLLALLLGDGNTKCCTGTGGCGGGCCACTTGGCCCCACCTGAAGAACGTGTTACGCTCC LPGRRGTRPNAFFYSKTGGCTCTTCTGTTGGGGGATGGGAACACAAAGCTGCCGGGTCGGAG DPELLAAYRRCAEALGAGGTACACGTCCTAATGCCTTCTTCTACAGCAAAGACCCCGAATTG AKVKAYVHPTTGVVTLCTCGCGGCTTATCGCCGGTGTGCAGAAGCCTTGGGTGCAAAGGTGA ATLAPRPGAQDPVKRLAAGCATACGTCCACCCGACTACGGGGGTGGTTACACTCGCAACCCT VVEAGMVAKAEEKRVPCGCTCCACGTCCTGGAGCTCAAGATCCTGTCAAACGCCTCGTTGTC EEVFRYRREALALFLGGAGGCGGGAATGGTTGCTAAAGCCGAAGAGAAGAGGGTCCCGGAGG RLFSTDGSVEKKRISYAGGTGTTTCGTTACCGGCGTGAGGCGTTGGCCCTTTTCTTGGGCCG SSASLGLAQDDAHLLLTTTGTTCTCGACAGACGGCTCTGTTGAAAAGAAGAGGATCTCTTAT RLGITSQLRSRGPRAHTCAAGTGCCAGTTTGGGACTGGCCCAGGATGACGCACATCTCTTGC EVLISGREDILRFAELTGCGCCTTGGAATTACATCTCAACTCCGTTCGAGAGGGCCACGGGC IGPYLLGAKRERLAALTCACGAGGTTCTTATATCGGGCCGCGAGGATATTTTGCGGTTTGCT EAEARRRLPGQGWHLRGAACTTATCGGACCCTACCTCTTGGGGGCCAAGAGGGAGAGACTTG LVLPAVAYRVSEAKRRCAGCGCTGGAAGCTGAGGCCCGCAGGCGTTTGCCTGGACAGGGATG SGFSWSEAGRRVAVAGGCACTTGCGGCTTGTTCTTCCTGCCGTGGCGTACAGAGTGAGCGAG SCLSSGLNLKLPRRYLGCTAAAAGGCGCTCGGGATTTTCGTGGAGTGAAGCCGGTCGGCGCG SRHRLSLLGEAFADPGTCGCAGTTGCGGGATCGTGTTTGTCATCTGGACTCAACCTCAAATT LEALAEGQVLWDPIVAGCCCAGACGCTACCTTTCTCGGCACCGGTTGTCGCTGCTCGGTGAG VEPAGKARTFDLRVPPGCTTTTGCCGACCCTGGGCTGGAAGCGCTCGCGGAAGGCCAAGTGC FANFVSEDLVVHNSIVTCTGGGACCCTATTGTTGCTGTCGAACCGGCCGGTAAGGCGAGAAC GTATFDQYWSVRTSKRATTCGACTTGCGCGTTCCACCCTTTGCAAACTTCGTGAGCGAGGAC TSGTVTVTDHFRAWANCTGGTGGTGCATAACTCCATTGTGGGGACAGCCACGTTCGATCAGT RGLNLGTIDQITLCVEACTGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAACAGTGACCGT GYQSSGSANITQNTFSGACCGATCACTTCCGCGCCTGGGCGAACCGGGGCCTGAACCTCGGC QGSSSGSSGGSSGSTTACAATAGACCAAATTACATTGTGCGTGGAGGGTTACCAAAGCTCTG TTRIECENMSLSGPYVGATCAGCCAACATCACCCAGAACACCTTCTCTCAGGGCTCTTCTTC SRITNPFNGIALYANGCGGCAGTTCGGGTGGCTCATCCGGCTCCACAACGACTACTCGCATC DTARATVNFPASRNYNGAGTGTGAGAACATGTCCTTGTCCGGACCCTACGTTAGCAGGATCA FRLRGCGNNNNLARVDCCAATCCCTTTAATGGTATTGCGCTGTACGCCAACGGAGACACAGC LRIDGRTVGTFYYQGTCCGCGCTACCGTTAACTTCCCCGCAAGTCGCAACTACAATTTCCGC YPWEAPIDNVYVSAGSCTGCGGGGTTGCGGCAACAACAATAATCTTGCCCGTGTGGACCTGA HTVEITVTADNGTWDVGGATCGACGGACGGACCGTCGGGACCTTTTATTACCAGGGCACATA YADYLVIQ* (SEQCCCCTGGGAGGCCCCAATTGACAATGTTTATGTCAGTGCGGGGAGT ID NO: 104)CATACAGTCGAAATCACTGTTACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACAGTGA (SEQ ID NO: 176) 2077 P77853S158-19MQTSITLTSNASGTFD ATGCAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTGGYYYELWKDTGNTTMT ACGGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAATVYTQGRFSCQWSNINN GACGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCGAACATCALFRTGKKYNQNWQSL AATAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTGGCGTIRITYSATYNPNGN AGTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAACCCSYLCIYGWSTNPLVEF AAACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACCCAYIVESWGNWRPPGATS TTGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACCGCLGQVTIDGGTYDIYRT CTGGTGCCACGTCCCTGGGCCAAGTGACAATCGATGGCGGGACCTATRVNQPCLAEGSLVLD CGACATCTATAGGACGACACGCGTCAACCAGCCTTGCCTGGCCGAGAATGQRVPIEKVRPGM GGCTCGCTCGTCTTGGACGCGGCTACCGGGCAGAGGGTCCCTATCGEVFSLGPDYRLYRVPV AAAAGGTGCGTCCGGGGATGGAAGTTTTCTCCTTGGGACCTGATTALEVLESGVGEVVRLRT CAGACTGTATCGGGTGCCCGTTTTGGAGGTCCTTGAGAGCGGGGTTRSGRTLVLTPDHPLLT GGGGAAGTTGTGCGCCTCAGAACTCGGTCAGGGAGAACGCTGGTGTPEGWKPLCDLPLGTPI TGACACCAGATCACCCGCTTTTGACCCCCGAAGGTTGGAAACCTCTAVPAELPVAGHLAPPE TTGTGACCTCCCGCTTGGAACTCCAATTGCAGTCCCCGCAGAACTGERVTLLALLLGDGNTK CCTGTGGCGGGCCACTTGGCCCCACCTGAAGAACGTGTTACGCTCCLSGRRGTRPIAFFYSK TGGCTCTTCTGTTGGGGGATGGGAACACAAAGCTGTCGGGTCGGAGDPELLAAYRRCAEALG AGGTACACGTCCTATTGCCTTCTTCTACAGCAAAGACCCCGAATTGAKVKAYVHPTTGVVTL CTCGCGGCTTATCGCCGGTGTGCAGAAGCCTTGGGTGCAAAGGTGAATLAPRPGAQDPVKRL AAGCATACGTCCACCCGACTACGGGGGTGGTTACACTCGCAACCCTVVEAGMVAKAEEKRVP CGCTCCACGTCCTGGAGCTCAAGATCCTGTCAAACGCCTCGTTGTCEEVFRYRREALALFLG GAGGCGGGAATGGTTGCTAAAGCCGAAGAGAAGAGGGTCCCGGAGGRLFSTDGSVEKKRISY AGGTGTTTCGTTACCGGCGTGAGGCGTTGGCCCTTTTCTTGGGCCGSSASLGLAQDVAHLLL TTTGTTCTCGACAGACGGCTCTGTTGAAAAGAAGAGGATCTCTTATRLGITSQLRSRGPRAH TCAAGTGCCAGTTTGGGACTGGCCCAGGATGTCGCACATCTCTTGCEVLISGREDILRFAEL TGCGCCTTGGAATTACATCTCAACTCCGTTCGAGAGGGCCACGGGCIGPYLLGAKRERLAAL TCACGAGGTTCTTATATCGGGCCGCGAGGATATTTTGCGGTTTGCTEAEARRRLPGQGWHLR GAACTTATCGGACCCTACCTCTTGGGGGCCAAGAGGGAGAGACTTGLVLPAVAYRVSEAKRR CAGCGCTGGAAGCTGAGGCCCGCAGGCGTTTGCCTGGACAGGGATGSGFSWSEAGRRVAVAG GCACTTGCGGCTTGTTCTTCCTGCCGTGGCGTACAGAGTGAGCGAGSCLSSGLNLKLPRRYL GCTAAAAGGCGCTCGGGATTTTCGTGGAGTGAAGCCGGTCGGCGCGSRHRLSLLGEAFADPG TCGCAGTTGCGGGATCGTGTTTGTCATCTGGACTCAACCTCAAATTLEALAEGQVLWDPIVA GCCCAGACGCTACCTTTCTCGGCACCGGTTGTCGCTGCTCGGTGAGVEPAGKARTFDLRVPP GCTTTTGCCGACCCTGGGCTGGAAGCGCTCGCGGAAGGCCAAGTGCFANFVSEDLVVHNSIV TCTGGGACCCTATTGTTGCTGTCGAACCGGCCGGTAAGGCGAGAACGTATFDQYWSVRTSKR ATTCGACTTGCGCGTTCCACCCTTTGCAAACTTCGTGAGCGAGGACTSGTVTVTDHFRAWAN CTGGTGGTGCATAACTCCATTGTGGGGACAGCCACGTTCGATCAGTRGLNLGTIDQITLCVE ACTGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAACAGTGACCGTGYQSSGSANITQNTFS GACCGATCACTTCCGCGCCTGGGCGAACCGGGGCCTGAACCTCGGCQGSSSGSSGGSSGSTT ACAATAGACCAAATTACATTGTGCGTGGAGGGTTACCAAAGCTCTGTTRIECENMSLSGPYV GATCAGCCAACATCACCCAGAACACCTTCTCTCAGGGCTCTTCTTCSRITNPFNGIALYANG CGGCAGTTCGGGTGGCTCATCCGGCTCCACAACGACTACTCGCATCDTARATVNFPASRNYN GAGTGTGAGAACATGTCCTTGTCCGGACCCTACGTTAGCAGGATCAFRLRGCGNNNNLARVD CCAATCCCTTTAATGGTATTGCGCTGTACGCCAACGGAGACACAGCLRIDGRTVGTFYYQGT CCGCGCTACCGTTAACTTCCCCGCAAGTCGCAACTACAATTTCCGCYPWEAPIDNVYVSAGS CTGCGGGGTTGCGGCAACAACAATAATCTTGCCCGTGTGGACCTGAHTVEITVTADNGTWDV GGATCGACGGACGGACCGTCGGGACCTTTTATTACCAGGGCACATAYADYLVIQ* (SEQ CCCCTGGGAGGCCCCAATTGACAATGTTTATGTCAGTGCGGGGAGT ID NO:105) CATACAGTCGAAATCACTGTTACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACAGTGA (SEQ ID NO: 177) 2078 P77853T134-1MQTSITLTSNASGTFD ATGCAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTGGYYYELWKDTGNTTMT ACGGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAATVYTQGRFSCQWSNINN GACGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCGAACATCALFRTGKKYNQNWQSL AATAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTGGCGTIRITYSATYNPNGN AGTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAACCCSYLCIYGWSTNPLVEF AAACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACCCAYIVESWGNWRPPGACL TTGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACCGCAEGSLVLDAATGQRVP CTGGTGCCTGCCTGGCCGAGGGCTCGCTCGTCTTGGACGCGGCTACIEKVRPGMEVFSLGPD CGGGCAGAGGGTCCCTATCGAAAAGGTGCGTCCGGGGATGGAAGTTYRLYRVPVLEVLESGV TTCTCCTTGGGACCTGATTACAGACTGTATCGGGTGCCCGTTTTGGREVVRLRTRSGRTLVL AGGTCCTTGAGAGCGGGGTTAGGGAAGTTGTGCGCCTCAGAACTCGTPDHPLLTPEGWKPLC GTCAGGGAGAACGCTGGTGTTGACACCAGATCACCCGCTTTTGACCDLPLGTPIAVPAELPV CCCGAAGGTTGGAAACCTCTTTGTGACCTCCCGCTTGGAACTCCAAACHLAPPEERVTLLAL TTGCAGTCCCCGCAGAACTGCCTGTGGCGTGCCACTTGGCCCCACCLLGDGNTKPSGRRGTR TGAAGAACGTGTTACGCTCCTGGCTCTTCTGTTGGGGGATGGGAACPNAFFYSKDPELLAAY ACAAAGCCGTCGGGTCGGAGAGGTACACGTCCTAATGCCTTCTTCTRRCAEALGAKVKAYVH ACAGCAAAGACCCCGAATTGCTCGCGGCTTATCGCCGGTGTGCAGAPTTGVVTLATLAPRPG AGCCTTGGGTGCAAAGGTGAAAGCATACGTCCACCCGACTACGGGGAQDPVKRLVVEAGMVA GTGGTTACACTCGCAACCCTCGCTCCACGTCCTGGAGCTCAAGATCKAEEKRVPEEVFRYRR CTGTCAAACGCCTCGTTGTCGAGGCGGGAATGGTTGCTAAAGCCGAEALALFLGRLFSTDGS AGAGAAGAGGGTCCCGGAGGAGGTGTTTCGTTACCGGCGTGAGGCGVEKKRISYSSASLGLA TTGGCCCTTTTCTTGGGCCGTTTGTTCTCGACAGACGGCTCTGTTGQDVAHLLLRLGITSQL AAAAGAAGAGGATCTCTTATTCAAGTGCCAGTTTGGGACTGGCCCARSRGPRAHEVLISGRE GGATGTCGCACATCTCTTGCTGCGCCTTGGAATTACATCTCAACTCDILRFAELIGPYLLGA CGTTCGAGAGGGCCACGGGCTCACGAGGTTCTTATATCGGGCCGCGKRERLAALEAEARRRL AGGATATTTTGCGGTTTGCTGAACTTATCGGACCCTACCTCTTGGGPGQGWHLRLVLPAVAY GGCCAAGAGGGAGAGACTTGCAGCGCTGGAAGCTGAGGCCCGCAGGRVSEAKRRSGESWSEA CGTTTGCCTGGACAGGGATGGCACTTGCGGCTTGTTCTTCCTGCCGGRRVAVAGSCLSSGLN TGGCGTACAGAGTGAGCGAGGCTAAAAGGCGCTCGGGATTTTCGTGLKLPRRYLSRHRLSLL GAGTGAAGCCGGTCGGCGCGTCGCAGTTGCGGGATCGTGTTTGTCAGEAFADPGLEALAEGQ TCTGGACTCAACCTCAAATTGCCCAGACGCTACCTTTCTCGGCACCVLWDPIVAVEPAGKAR GGTTGTCGCTGCTCGGTGAGGCTTTTGCCGACCCTGGGCTGGAAGCTFDLRVPPFANFVSED GCTCGCGGAAGGCCAAGTGCTCTGGGACCCTATTGTTGCTGTCGAALVVHNTSPLGQVTIDG CCGGCCGGTAAGGCGAGAACATTCGACTTGCGCGTTCCACCCTTTGGTYDIYRTTRVNQPSI CAAACTTCGTGAGCGAGGACCTGGTGGTGCATAACACGTCCCCCTTVGTATFDQYWSVRTSK GGGCCAAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGRTSGTVTVTDHFRAWA ACACGCGTCAACCAGCCTTCCATTGTGGGGACAGCCACGTTCGATCNRGLNLGTIDQITLCV AGTACTGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAACAGTGACEGYQSSGSANITQNTF CGTGACCGATCACTTCCGCGCCTGGGCGAACCGGGGCCTGAACCTCSQGSSSGSSGGSSGST GGCACAATAGACCAAATTACATTGTGCGTGGAGGGTTACCAAAGCTTTTRIECENMSLSGPY CTGGATCAGCCAACATCACCCAGAACACCTTCTCTCAGGGCTCTTCVSRITNPFNGIALYAN TTCCGGCAGTTCGGGTGGCTCATCCGGCTCCACAACGACTACTCGCGDTARATVNFPASRNY ATCGAGTGTGAGAACATGTCCTTGTCCGGACCCTACGTTAGCAGGANFRLRGCGNNNNLARV TCACCAATCCCTTTAATGGTATTGCGCTGTACGCCAACGGAGACACDLRIDGRTVGTFYYQG AGCCCGCGCTACCGTTAACTTCCCCGCAAGTCGCAACTACAATTTCTYPWEAPIDNVYVSAG CGCCTGCGGGGTTGCGGCAACAACAATAATCTTGCCCGTGTGGACCSHTVEITVTADNGTWD TGAGGATCGACGGACGGACCGTCGGGACCTTTTATTACCAGGGCACVYADYLVIQ* ATACCCCTGGGAGGCCCCAATTGACAATGTTTATGTCAGTGCGGGG (SEQ ID NO:106) AGTCATACAGTCGAAATCACTGTTACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACAGTGA (SEQ ID NO: 178) 2079 BAASS:P77853S158-MANKHLSLSLFLVLLG ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG 2:SEKDELLSASLASGQQTSITLT GCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCTSNASGTFDGYYYELWK GACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTCDTGNTTMTVYTQGRFS TGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTCCQWSNINNALFRTGKK GCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGACYNQNWQSLGTIRITYS CGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGGATYNPNGNSYLCIYGW ATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGTSTNPLVEFYIVESWGN GTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGTWRPPGATSLGQVTIDG TGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTGGGCGTYDIYRTTRVNQPCL CAAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGACACAEGSLVLDAATGQRVP GCGTCAACCAGCCTTGCCTGGCCGAGGGCTCGCTCGTCTTGGACGCIEKVRPGMEVFSLGPD GGCTACCGGGCAGAGGGTCCCTATCGAAAAGGTGCGTCCGGGGATGYRLYRVPVLEVLESGV GAAGTTTTCTCCTTGGGACCTGATTACAGACTGTATCGGGTGCCCGREVVRLRTRSGRTLVL TTTTGGAGGTCCTTGAGAGCGGGGTTAGGGAAGTTGTGCGCCTCAGTPDHPLLTPEGWKPLC AACTCGGTCAGGGAGAACGCTGGTGTTGACACCAGATCACCCGCTTDLPLGTPIAVPAELPV TTGACCCCCGAAGGTTGGAAACCTCTTTGTGACCTCCCGCTTGGAAAGHLAPPEERVTLLAL CTCCAATTGCAGTCCCCGCAGAACTGCCTGTGGCGGGCCACTTGGCLLGDGNTKLPGRRGTR CCCACCTGAAGAACGTGTTACGCTCCTGGCTCTTCTGTTGGGGGATPNAFFYSKDPELLAAY GGGAACACAAAGCTGCCGGGTCGGAGAGGTACACGTCCTAATGCCTRRCAEALGAKVKAYVH TCTTCTACAGCAAAGACCCCGAATTGCTCGCGGCTTATCGCCGGTGPTTGVVTLATLAPRPG TGCAGAAGCCTTGGGTGCAAAGGTGAAAGCATACGTCCACCCGACTAQDPVKRLVVEAGMVA ACGGGGGTGGTTACACTCGCAACCCTCGCTCCACGTCCTGGAGCTCKAEEKRVPEEVFRYRR AAGATCCTGTCAAACGCCTCGTTGTCGAGGCGGGAATGGTTGCTAAEALALFLGRLFSTDGS AGCCGAAGAGAAGAGGGTCCCGGAGGAGGTGTTTCGTTACCGGCGTVEKKRISYSSASLGLA GAGGCGTTGGCCCTTTTCTTGGGCCGTTTGTTCTCGACAGACGGCTQDDAHLLLRLGITSQL CTGTTGAAAAGAAGAGGATCTCTTATTCAAGTGCCAGTTTGGGACTRSRGPRAHEVLISGRE GGCCCAGGATGACGCACATCTCTTGCTGCGCCTTGGAATTACATCTDILRFAELIGPYLLGA CAACTCCGTTCGAGAGGGCCACGGGCTCACGAGGTTCTTATATCGGKRERLAALEAEARRRL GCCGCGAGGATATTTTGCGGTTTGCTGAACTTATCGGACCCTACCTPGQGWHLRLVLPAVAY CTTGGGGGCCAAGAGGGAGAGACTTGCAGCGCTGGAAGCTGAGGCCRVSEAKRRSGFSWSEA CGCAGGCGTTTGCCTGGACAGGGATGGCACTTGCGGCTTGTTCTTCGRRVAVAGSCLSSGLN CTGCCGTGGCGTACAGAGTGAGCGAGGCTAAAAGGCGCTCGGGATTLKLPRRYLSRHRLSLL TTCGTGGAGTGAAGCCGGTCGGCGCGTCGCAGTTGCGGGATCGTGTGEAFADPGLEALAEGQ TTGTCATCTGGACTCAACCTCAAATTGCCCAGACGCTACCTTTCTCVLWDPIVAVEPAGKAR GGCACCGGTTGTCGCTGCTCGGTGAGGCTTTTGCCGACCCTGGGCTTFDLRVPPFANFVSED GGAAGCGCTCGCGGAAGGCCAAGTGCTCTGGGACCCTATTGTTGCTLVVHNSIVGTATFDQY GTCGAACCGGCCGGTAAGGCGAGAACATTCGACTTGCGCGTTCCACWSVRTSKRTSGTVTVT CCTTTGCAAACTTCGTGAGCGAGGACCTGGTGGTGCATAACTCCATDHFRAWANRGLNLGTI TGTGGGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCTDQITLCVEGYQSSGSA AAGCGGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCTNITQNTFSQGSSSGSS GGGCGAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTACATTGGSSGSTTTTRIECEN GTGCGTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAGMSLSGPYVSRITNPFN AACACCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCATGIALYANGDTARATVN CCGGCTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTCCTTFPASRNYNFRLRGCGN GTCCGGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATTNNNLARVDLRIDGRTV GCGCTGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCCGTFYYQGTYPWEAPID CCGCAAGTCGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAANVYVSAGSHTVEITVT CAATAATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTCADNGTWDVYADYLVIQ GGGACCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAATTG SEKDEL*(SEQ ACAATGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGT ID NO: 107)TACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACAGAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 179) 2080 BAASS:P77853S158-MANKHLSLSLFLVLLG ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG19:SEKDEL LSASLASGQQTSITLTGCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCT SNASGTFDGYYYELWKGACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTC DTGNTTMTVYTQGRFSTGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTC CQWSNINNALFRTGKKGCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGAC YNQNWQSLGTIRITYSCGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGG ATYNPNGNSYLCIYGWATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGT STNPLVEFYIVESWGNGTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGT WRPPGATSLGQVTIDGTGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTGGGC GTYDIYRTTRVNQPCLCAAGTGACAATCGATGGCGGGACCTACGACATCTATAGGACGACAC AEGSLVLDAATGQRVPGCGTCAACCAGCCTTGCCTGGCCGAGGGCTCGCTCGTCTTGGACGC IEKVRPGMEVFSLGPDGGCTACCGGGCAGAGGGTCCCTATCGAAAAGGTGCGTCCGGGGATG YRLYRVPVLEVLESGVGAAGTTTTCTCCTTGGGACCTGATTACAGACTGTATCGGGTGCCCG GEVVRLRTRSGRTLVLTTTTGGAGGTCCTTGAGAGCGGGGTTGGGGAAGTTGTGCGCCTCAG TPDHPLLTPEGWKPLCAACTCGGTCAGGGAGAACGCTGGTGTTGACACCAGATCACCCGCTT DLPLGTPIAVPAELPVTTGACCCCCGAAGGTTGGAAACCTCTTTGTGACCTCCCGCTTGGAA AGHLAPPEERVTLLALCTCCAATTGCAGTCCCCGCAGAACTGCCTGTGGCGGGCCACTTGGC LLGDGNTKLSGRRGTRCCCACCTGAAGAACGTGTTACGCTCCTGGCTCTTCTGTTGGGGGAT PIAFFYSKDPELLAAYGGGAACACAAAGCTGTCGGGTCGGAGAGGTACACGTCCTATTGCCT RRCAEALGAKVKAYVHTCTTCTACAGCAAAGACCCCGAATTGCTCGCGGCTTATCGCCGGTG PTTGVVTLATLAPRPGTGCAGAAGCCTTGGGTGCAAAGGTGAAAGCATACGTCCACCCGACT AQDPVKRLVVEAGMVAACGGGGGTGGTTACACTCGCAACCCTCGCTCCACGTCCTGGAGCTC KAEEKRVPEEVFRYRRAAGATCCTGTCAAACGCCTCGTTGTCGAGGCGGGAATGGTTGCTAA EALALFLGRLFSTDGSAGCCGAAGAGAAGAGGGTCCCGGAGGAGGTGTTTCGTTACCGGCGT VEKKRISYSSASLGLAGAGGCGTTGGCCCTTTTCTTGGGCCGTTTGTTCTCGACAGACGGCT QDVAHLLLRLGITSQLCTGTTGAAAAGAAGAGGATCTCTTATTCAAGTGCCAGTTTGGGACT RSRGPRAHEVLISGREGGCCCAGGATGTCGCACATCTCTTGCTGCGCCTTGGAATTACATCT DILRFAELIGPYLLGACAACTCCGTTCGAGAGGGCCACGGGCTCACGAGGTTCTTATATCGG KRERLAALEAEARRRLGCCGCGAGGATATTTTGCGGTTTGCTGAACTTATCGGACCCTACCT PGQGWHLRLVLPAVAYCTTGGGGGCCAAGAGGGAGAGACTTGCAGCGCTGGAAGCTGAGGCC RVSEAKRRSGFSWSEACGCAGGCGTTTGCCTGGACAGGGATGGCACTTGCGGCTTGTTCTTC GRRVAVAGSCLSSGLNCTGCCGTGGCGTACAGAGTGAGCGAGGCTAAAAGGCGCTCGGGATT LKLPRRYLSRHRLSLLTTCGTGGAGTGAAGCCGGTCGGCGCGTCGCAGTTGCGGGATCGTGT GEAFADPGLEALAEGQTTGTCATCTGGACTCAACCTCAAATTGCCCAGACGCTACCTTTCTC VLWDPIVAVEPAGKARGGCACCGGTTGTCGCTGCTCGGTGAGGCTTTTGCCGACCCTGGGCT TFDLRVPPFANFVSEDGGAAGCGCTCGCGGAAGGCCAAGTGCTCTGGGACCCTATTGTTGCT LVVHNSIVGTATFDQYGTCGAACCGGCCGGTAAGGCGAGAACATTCGACTTGCGCGTTCCAC WSVRTSKRTSGTVTVTCCTTTGCAAACTTCGTGAGCGAGGACCTGGTGGTGCATAACTCCAT DHFRAWANRGLNLGTITGTGGGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCTCT DQITLCVEGYQSSGSAAAGCGGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGCCT NITQNTFSQGSSSGSSGGGCGAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTACATT GGSSGSTTTTRIECENGTGCGTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCCAG MSLSGPYVSRITNPFNAACACCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTCAT GIALYANGDTARATVNCCGGCTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTCCTT FPASRNYNFRLRGCGNGTCCGGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTATT NNNLARVDLRIDGRTVGCGCTGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTTCC GTFYYQGTYPWEAPIDCCGCAAGTCGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAACAA NVYVSAGSHTVEITVTCAATAATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCGTC ADNGTWDVYADYLVIQGGGACCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAATTG SEKDEL* (SEQACAATGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCACTGT ID NO: 108)TACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACAGAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 180) 2081 BAASS:P77853T143-MANKHLSLSLFLVLLG ATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG 1:SEKDELLSASLASGQQTSITLT GCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCTSNASGTFDGYYYELWK GACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTCDTGNTTMTVYTQGRFS TGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTCCQWSNINNALFRTGKK GCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGACYNQNWQSLGTIRITYS CGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGGATYNPNGNSYLCIYGW ATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGTSTNPLVEFYIVESWGN GTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGTWRPPGACLAEGSLVLD TGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCTGCCTGGCCGAGAATGQRVPIEKVRPGM GGCTCGCTCGTCTTGGACGCGGCTACCGGGCAGAGGGTCCCTATCGEVFSLGPDYRLYRVPV AAAAGGTGCGTCCGGGGATGGAAGTTTTCTCCTTGGGACCTGATTALEVLESGVREVVRLRT CAGACTGTATCGGGTGCCCGTTTTGGAGGTCCTTGAGAGCGGGGTTRSGRTLVLTPDHPLLT AGGGAAGTTGTGCGCCTCAGAACTCGGTCAGGGAGAACGCTGGTGTPEGWKPLCDLPLGTPI TGACACCAGATCACCCGCTTTTGACCCCCGAAGGTTGGAAACCTCTAVPAELPVACHLAPPE TTGTGACCTCCCGCTTGGAACTCCAATTGCAGTCCCCGCAGAACTGERVTLLALLLGDGNTK CCTGTGGCGTGCCACTTGGCCCCACCTGAAGAACGTGTTACGCTCCPSGRRGTRPNAFFYSK TGGCTCTTCTGTTGGGGGATGGGAACACAAAGCCGTCGGGTCGGAGDPELLAAYRRCAEALG AGGTACACGTCCTAATGCCTTCTTCTACAGCAAAGACCCCGAATTGAKVKAYVHPTTGVVTL CTCGCGGCTTATCGCCGGTGTGCAGAAGCCTTGGGTGCAAAGGTGAATLAPRPGAQDPVKRL AAGCATACGTCCACCCGACTACGGGGGTGGTTACACTCGCAACCCTVVEAGMVAKAEEKRVP CGCTCCACGTCCTGGAGCTCAAGATCCTGTCAAACGCCTCGTTGTCEEVFRYRREALALFLG GAGGCGGGAATGGTTGCTAAAGCCGAAGAGAAGAGGGTCCCGGAGGRLFSTDGSVEKKRISY AGGTGTTTCGTTACCGGCGTGAGGCGTTGGCCCTTTTCTTGGGCCGSSASLGLAQDVAHLLL TTTGTTCTCGACAGACGGCTCTGTTGAAAAGAAGAGGATCTCTTATRLGITSQLRSRGPRAH TCAAGTGCCAGTTTGGGACTGGCCCAGGATGTCGCACATCTCTTGCEVLISGREDILRFAEL TGCGCCTTGGAATTACATCTCAACTCCGTTCGAGAGGGCCACGGGCIGPYLLGAKRERLAAL TCACGAGGTTCTTATATCGGGCCGCGAGGATATTTTGCGGTTTGCTEAEARRRLPGQGWHLR GAACTTATCGGACCCTACCTCTTGGGGGCCAAGAGGGAGAGACTTGLVLPAVAYRVSEAKRR CAGCGCTGGAAGCTGAGGCCCGCAGGCGTTTGCCTGGACAGGGATGSGFSWSEAGRRVAVAG GCACTTGCGGCTTGTTCTTCCTGCCGTGGCGTACAGAGTGAGCGAGSCLSSGLNLKLPRRYL GCTAAAAGGCGCTCGGGATTTTCGTGGAGTGAAGCCGGTCGGCGCGSRHRLSLLGEAFADPG TCGCAGTTGCGGGATCGTGTTTGTCATCTGGACTCAACCTCAAATTLEALAEGQVLWDPIVA GCCCAGACGCTACCTTTCTCGGCACCGGTTGTCGCTGCTCGGTGAGVEPAGKARTFDLRVPP GCTTTTGCCGACCCTGGGCTGGAAGCGCTCGCGGAAGGCCAAGTGCFANFVSEDLVVHNTSP TCTGGGACCCTATTGTTGCTGTCGAACCGGCCGGTAAGGCGAGAACLGQVTIDGGTYDIYRT ATTCGACTTGCGCGTTCCACCCTTTGCAAACTTCGTGAGCGAGGACTRVNQPSIVGTATFDQ CTGGTGGTGCATAACACGTCCCCCTTGGGCCAAGTGACAATCGATGYWSVRTSKRTSGTVTV GCGGGACCTACGACATCTATAGGACGACACGCGTCAACCAGCCTTCTDHFRAWANRGLNLGT CATTGTGGGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCACCIDQITLCVEGYQSSGS TCTAAGCGGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGCGANITQNTFSQGSSSGS CCTGGGCGAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTACSGGSSGSTTTTRIECE ATTGTGCGTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCACCNMSLSGPYVSRITNPF CAGAACACCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGCTNGIALYANGDTARATV CATCCGGCTCCACAACGACTACTCGCATCGAGTGTGAGAACATGTCNFPASRNYNFRLRGCG CTTGTCCGGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGGTNNNNLARVDLRIDGRT ATTGCGCTGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAACTVGTFYYQGTYPWEAPI TCCCCGCAAGTCGCAACTACAATTTCCGCCTGCGGGGTTGCGGCAADNVYVSAGSHTVEITV CAACAATAATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGACCTADNGTWDVYADYLVI GTCGGGACCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCAA QSEKDEL*(SEQ TTGACAATGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCAC ID NO: 109)TGTTACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCTGGTGATACAGAGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 181) 2082 GluB4SP:O43097MATIAFSRLSIYFCVL ATGGCCACCATCGCTTTCTCCCGCTTGTCCATCTACTTCTGCGTGCLLCHGSMAFPAGNATE TTCTCCTGTGCCACGGCTCCATGGCCTTCCCAGCTGGAAACGCAACLEKRQTTPNSEGWHDG GGAATTGGAGAAAAGACAAACCACCCCTAACTCTGAGGGCTGGCATYYYSWWSDGGAQATYT GACGGATACTACTACTCTTGGTGGAGCGATGGTGGTGCACAGGCCANLEGGTYEISWGDGGN CCTATACAAACCTCGAAGGCGGCACTTATGAGATTTCATGGGGTGALVGGKGWNPGLNARAI CGGTGGCAACCTTGTCGGCGGAAAGGGGTGGAACCCCGGACTTAACHFEGVYQPNGNSYLAV GCCAGGGCAATCCACTTCGAAGGGGTGTACCAGCCCAATGGCAACTYGWTRNPLVEYYIVEN CATACCTGGCCGTCTACGGGTGGACGCGCAATCCGCTGGTTGAGTAFGTYDPSSGATDLGTV CTATATCGTGGAGAATTTCGGAACTTATGACCCTAGCTCCGGTGCCECDGSIYRLGKTTRVN ACGGACCTCGGGACAGTCGAGTGTGACGGAAGCATCTACAGGCTGGAPSIDGTQTFDQYWSV GTAAAACTACCCGCGTTAATGCTCCATCGATCGACGGCACGCAAACRQDKRTSGTVQTGCHF ATTTGATCAATACTGGTCCGTGCGGCAGGATAAGAGGACAAGCGGCDAWARAGLNVNGDHYY ACAGTTCAGACGGGTTGCCACTTTGATGCCTGGGCAAGAGCGGGGCQIVATEGYFSSGYARI TCAATGTGAATGGGGACCACTACTATCAGATTGTGGCGACCGAGGG TVADVG*(SEQ CTATTTCTCCAGTGGCTATGCGCGTATAACCGTCGCTGATGTTGGA ID NO: 110) TGA (SEQID NO: 182) 2083 GluB4SP:O43097: MATIAFSRLSIYFCVLATGGCCACCATCGCTTTCTCCCGCTTGTCCATCTACTTCTGCGTGC SEKDEL LLCHGSMAFPAGNATETTCTCCTGTGCCACGGCTCCATGGCCTTCCCAGCTGGAAACGCAAC LEKRQTTPNSEGWHDGGGAATTGGAGAAAAGACAAACCACCCCTAACTCTGAGGGCTGGCAT YYYSWWSDGGAQATYTGACGGATACTACTACTCTTGGTGGAGCGATGGTGGTGCACAGGCCA NLEGGTYEISWGDGGNCCTATACAAACCTCGAAGGCGGCACTTATGAGATTTCATGGGGTGA LVGGKGWNPGLNARAICGGTGGCAACCTTGTCGGCGGAAAGGGGTGGAACCCCGGACTTAAC HFEGVYQPNGNSYLAVGCCAGGGCAATCCACTTCGAAGGGGTGTACCAGCCCAATGGCAACT YGWTRNPLVEYYIVENCATACCTGGCCGTCTACGGGTGGACGCGCAATCCGCTGGTTGAGTA FGTYDPSSGATDLGTVCTATATCGTGGAGAATTTCGGAACTTATGACCCTAGCTCCGGTGCC ECDGSIYRLGKTTRVNACGGACCTCGGGACAGTCGAGTGTGACGGAAGCATCTACAGGCTGG APSIDGTQTFDQYWSVGTAAAACTACCCGCGTTAATGCTCCATCGATCGACGGCACGCAAAC RQDKRTSGTVQTGCHFATTTGATCAATACTGGTCCGTGCGGCAGGATAAGAGGACAAGCGGC DAWARAGLNVNGDHYYACAGTTCAGACGGGTTGCCACTTTGATGCCTGGGCAAGAGCGGGGC QIVATEGYFSSGYARITCAATGTGAATGGGGACCACTACTATCAGATTGTGGCGACCGAGGG TVADVGSEKDEL*CTATTTCTCCAGTGGCTATGCGCGTATAACCGTCGCTGATGTTGGA (SEQ ID NO: 111)AGCGAGAAGGACGAGCTGTGA (SEQ ID NO: 183) 2084 GluB4SP:NtEGmMATIAFSRLSIYFCVL ATGGCCACCATCGCTTTCTCCCGCTTGTCCATCTACTTCTGCGTGCLLCHGSMAAYDYKQVL TTCTCCTGTGCCACGGCTCCATGGCCGCTTACGACTACAAGCAGGTRDSLLFYEAQRSGRLP GTTGCGGGACTCGCTACTATTCTATGAGGCCCAGAGATCCGGCCGGADQKVTWRKDSALNDQ CTCCCAGCCGACCAGAAGGTCACGTGGAGGAAGGATAGCGCGCTGAGDQGQDLTGGYFDAGD ATGACCAGGGTGACCAGGGACAAGACTTGACCGGCGGCTACTTTGAFVKFGFPMAYTATVLA CGCTGGGGACTTCGTCAAGTTCGGGTTCCCCATGGCTTATACCGCAWGLIDFEAGYSSAGAL ACCGTGCTGGCATGGGGCCTCATAGATTTTGAGGCCGGCTACAGCADDGRKAVKWATDYFIK GTGCCGGGGCCTTGGATGATGGACGGAAGGCTGTCAAATGGGCCACAHTSQNEFYGQVGQGD CGACTATTTCATAAAGGCCCACACAAGTCAAAATGAGTTCTATGGTADHAFWGRPEDMTMAR CAGGTCGGCCAGGGTGACGCCGATCACGCTTTCTGGGGAAGACCAGPAYKIDTSRPGSDLAG AGGATATGACGATGGCGCGCCGGGCGTACAAGATAGACACCTCAAGETAAALAAASIVFRNV GCCTGGCTCTGATCTGGCAGGCGAGACAGCGGCTGCTCTTGCCGCTDGTYSNNLLTHARQLF GCTTCAATCGTGTTCCGGAACGTCGATGGCACTTACTCAAATAACCDFANNYRGKYSDSITD TGTTAACACACGCTCGCCAGCTATTCGACTTCGCGAACAACTACCGARNFYASADYRDELVW GGGAAAGTATAGTGACTCTATTACTGACGCAAGAAATTTCTACGCAAAAWLYRATNDNTYLN AGCGCAGACTACAGAGACGAGTTGGTTTGGGCTGCTGCGTGGTTATTAESLYDEFGLQNWGG ACAGAGCGACCAACGACAACACCTACCTCAACACTGCTGAGTCACTGLNWDSKVSGVQVLLA GTACGATGAGTTTGGGCTACAGAACTGGGGGGGGGGCCTGAACTGGKLTNKQAYKDTVQSYV GATAGCAAGGTGTCTGGCGTGCAGGTGTTGTTGGCCAAGCTTACCANYLINNQQKTPKGLLY ATAAGCAGGCCTACAAGGACACGGTGCAGTCTTACGTCAATTACCTIDMWGTLRHAANAAFI AATTAATAACCAGCAGAAGACTCCCAAGGGCCTCCTCTACATCGACMLEAAELGLSASSYRQ ATGTGGGGCACCCTTCGCCACGCTGCCAACGCCGCATTCATCATGCFAQTQIDYALGDGGRS TCGAAGCCGCCGAGCTGGGCTTGTCCGCCTCCTCTTATAGACAGTTFVCGFGSNPPTRPHHR CGCGCAAACGCAAATCGACTACGCCCTGGGCGATGGTGGCCGCTCCSSSCPPAPATCDWNTF TTTGTGTGCGGGTTCGGGAGTAATCCTCCTACGAGACCGCACCACANSPDPNYHVLSGALVG GATCCTCGTCGTGCCCGCCAGCTCCCGCTACTTGCGACTGGAATACGPDQNDNYVDDRSDYV ATTCAACTCACCTGACCCAAACTACCACGTCCTCTCTGGGGCCCTAHNEVATDYNAGFQSAL GTGGGCGGACCTGATCAGAATGACAACTACGTCGATGACCGTTCAGAALVALGY* (SEQ ACTATGTTCACAACGAAGTCGCCACTGATTACAACGCGGGTTTCCA ID NO:112) GTCCGCGTTAGCTGCTTTGGTGGCCCTTGGTTACTGA (SEQ ID NO: 184) 2085P77853T145-307 MQTSITLTSNASGTFDATGCAAACAAGCATTACTCTGACATCCAACGCATCCGGTACGTTTG GYYYELWKDTGNTTMTACGGTTACTATTACGAACTCTGGAAGGATACTGGCAATACAACAAT VYTQGRFSCQWSNINNGACGGTCTACACTCAAGGTCGCTTTTCCTGCCAGTGGTCGAACATC ALFRTGKKYNQNWQSLAATAACGCGTTGTTTAGGACCGGGAAGAAATACAACCAGAATTGGC GTIRITYSATYNPNGNAGTCTCTTGGCACAATCCGGATCACGTACTCTGCGACTTACAACCC SYLCIYGWSTNPLVEFAAACGGGAACTCCTACTTGTGTATCTATGGCTGGTCTACCAACCCA YIVESWGNWRPPGATSTTGGTCGAGTTCTACATCGTTGAGTCCTGGGGGAACTGGAGACCGC LGQVTIDGGSVTGDTECTGGTGCCACGTCCCTGGGCCAAGTGACAATCGATGGCGGGAGCGT IIVKRNGRIEFVPIEKTACTGGAGACACCGAAATTATCGTCAAGAGAAATGGTAGGATCGAA LFERVDYRIGEKEYCITTTGTCCCGATCGAGAAGCTCTTTGAGAGAGTGGACTATAGAATAG LEDVEALTLDNRDKLIGCGAGAAAGAATACTGCATCCTTGAGGACGTTGAGGCGCTGACTCT WKKVPYVMRHRAKKKVTGACAACAGAGACAAACTTATTTGGAAGAAGGTGCCCTACGTCATG YRIWITNSWYIDVTEDCGTCACAGGGCAAAGAAAAAGGTCTACCGTATCTGGATTACTAATT HSLIVAEDGLKEARPMCATGGTACATAGACGTTACAGAGGACCACTCCCTGATTGTGGCTGA EIEGKSLIATKDDLSGGGACGGGCTGAAGGAGGCCCGCCCCATGGAAATTGAGGGCAAGTCT VEYIKPHAIEEISYNGCTGATTGCAACTAAAGATGATCTCTCTGGCGTTGAGTACATCAAGC YVYDIEVEGTHRFFANCTCACGCTATTGAGGAGATTAGTTACAACGGTTACGTGTACGATAT GILVHNTYDIYRTTRVCGAAGTGGAGGGTACTCATAGATTCTTCGCTAATGGGATACTGGTG NQPSIVGTATFDQYWSCATAACACCTACGACATCTATAGGACGACACGCGTCAACCAGCCTT VRTSKRTSGTVTVTDHCCATTGTGGGGACAGCCACGTTCGATCAGTACTGGAGCGTGCGCAC FRAWANRGLNLGTIDQCTCTAAGCGGACTTCAGGAACAGTGACCGTGACCGATCACTTCCGC ITLCVEGYQSSGSANIGCCTGGGCGAACCGGGGCCTGAACCTCGGCACAATAGACCAAATTA TQNTFSQGSSSGSSGGCATTGTGCGTGGAGGGTTACCAAAGCTCTGGATCAGCCAACATCAC SSGSTTTTRIECENMSCCAGAACACCTTCTCTCAGGGCTCTTCTTCCGGCAGTTCGGGTGGC LSGPYVSRITNPFNGITCATCCGGCTCCACAACGACTACTCGCATCGAGTGTGAGAACATGT ALYANGDTARATVNFPCCTTGTCCGGACCCTACGTTAGCAGGATCACCAATCCCTTTAATGG ASRNYNFRLRGCGNNNTATTGCGCTGTACGCCAACGGAGACACAGCCCGCGCTACCGTTAAC NLARVDLRIDGRTVGTTTCCCCGCAAGTCGCAACTACAATTTCCGCCTGCGGGGTTGCGGCA FYYQGTYPWEAPIDNVACAACAATAATCTTGCCCGTGTGGACCTGAGGATCGACGGACGGAC YVSAGSHTVEITVTADCGTCGGGACCTTTTATTACCAGGGCACATACCCCTGGGAGGCCCCA NGTWDVYADYLVIQ*ATTGACAATGTTTATGTCAGTGCGGGGAGTCATACAGTCGAAATCA (SEQ ID NO: 113)CTGTTACTGCGGATAACGGCACATGGGACGTGTATGCCGACTACCT GGTGATACAGTGA (SEQ ID NO:185) 2086 BAASS:P77853T145- MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG 307 LSASLASGQQTSITLTGCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCT SNASGTFDGYYYELWKGACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTC DTGNTTMTVYTQGRFSTGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTC CQWSNINNALFRTGKKGCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGAC YNQNWQSLGTIRITYSCGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGG ATYNPNGNSYLCIYGWATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGT STNPLVEFYIVESWGNGTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGT WRPPGATSLGQVTIDGTGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTGGGC GSVTGDTEIIVKRNGRCAAGTGACAATCGATGGCGGGAGCGTTACTGGAGACACCGAAATTA IEFVPIEKLFERVDYRTCGTCAAGAGAAATGGTAGGATCGAATTTGTCCCGATCGAGAAGCT IGEKEYCILEDVEALTCTTTGAGAGAGTGGACTATAGAATAGGCGAGAAAGAATACTGCATC LDNRDKLIWKKVPYVMCTTGAGGACGTTGAGGCGCTGACTCTTGACAACAGAGACAAACTTA RHRAKKKVYRIWITNSTTTGGAAGAAGGTGCCCTACGTCATGCGTCACAGGGCAAAGAAAAA WYIDVTEDHSLIVAEDGGTCTACCGTATCTGGATTACTAATTCATGGTACATAGACGTTACA GLKEARPMEIEGKSLIGAGGACCACTCCCTGATTGTGGCTGAGGACGGGCTGAAGGAGGCCC ATKDDLSGVEYIKPHAGCCCCATGGAAATTGAGGGCAAGTCTCTGATTGCAACTAAAGATGA IEEISYNGYVYDIEVETCTCTCTGGCGTTGAGTACATCAAGCCTCACGCTATTGAGGAGATT GTHRFFANGILVHNTYAGTTACAACGGTTACGTGTACGATATCGAAGTGGAGGGTACTCATA DIYRTTRVNQPSIVGTGATTCTTCGCTAATGGGATACTGGTGCATAACACCTACGACATCTA ATFDQYWSVRTSKRTSTAGGACGACACGCGTCAACCAGCCTTCCATTGTGGGGACAGCCACG GTVTVTDHFRAWANRGTTCGATCAGTACTGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAA LNLGTIDQITLCVEGYCAGTGACCGTGACCGATCACTTCCGCGCCTGGGCGAACCGGGGCCT QSSGSANITQNTFSQGGAACCTCGGCACAATAGACCAAATTACATTGTGCGTGGAGGGTTAC SSSGSSGGSSGSTTTTCAAAGCTCTGGATCAGCCAACATCACCCAGAACACCTTCTCTCAGG RIECENMSLSGPYVSRGCTCTTCTTCCGGCAGTTCGGGTGGCTCATCCGGCTCCACAACGAC ITNPFNGIALYANGDTTACTCGCATCGAGTGTGAGAACATGTCCTTGTCCGGACCCTACGTT ARATVNFPASRNYNFRAGCAGGATCACCAATCCCTTTAATGGTATTGCGCTGTACGCCAACG LRGCGNNNNLARVDLRGAGACACAGCCCGCGCTACCGTTAACTTCCCCGCAAGTCGCAACTA IDGRTVGTFYYQGTYPCAATTTCCGCCTGCGGGGTTGCGGCAACAACAATAATCTTGCCCGT WEAPIDNVYVSAGSHTGTGGACCTGAGGATCGACGGACGGACCGTCGGGACCTTTTATTACC VEITVTADNGTWDVYAAGGGCACATACCCCTGGGAGGCCCCAATTGACAATGTTTATGTCAG DYLVIQ* (SEQTGCGGGGAGTCATACAGTCGAAATCACTGTTACTGCGGATAACGGC ID NO: 114)ACATGGGACGTGTATGCCGACTACCTGGTGATACAGTGA (SEQ ID NO: 186) 2087BAASS:P77853T145- MANKHLSLSLFLVLLGATGGCGAACAAACATTTGTCCCTCTCCCTCTTCCTCGTCCTCCTTG 307:SEKDELLSASLASGQQTSITLT GCCTGTCGGCCAGCTTGGCCTCCGGGCAACAAACAAGCATTACTCTSNASGTFDGYYYELWK GACATCCAACGCATCCGGTACGTTTGACGGTTACTATTACGAACTCDTGNTTMTVYTQGRFS TGGAAGGATACTGGCAATACAACAATGACGGTCTACACTCAAGGTCCQWSNINNALFRTGKK GCTTTTCCTGCCAGTGGTCGAACATCAATAACGCGTTGTTTAGGACYNQNWQSLGTIRITYS CGGGAAGAAATACAACCAGAATTGGCAGTCTCTTGGCACAATCCGGATYNPNGNSYLCIYGW ATCACGTACTCTGCGACTTACAACCCAAACGGGAACTCCTACTTGTSTNPLVEFYIVESWGN GTATCTATGGCTGGTCTACCAACCCATTGGTCGAGTTCTACATCGTWRPPGATSLGQVTIDG TGAGTCCTGGGGGAACTGGAGACCGCCTGGTGCCACGTCCCTGGGCGSVTGDTEIIVKRNGR CAAGTGACAATCGATGGCGGGAGCGTTACTGGAGACACCGAAATTAIEFVPIEKLFERVDYR TCGTCAAGAGAAATGGTAGGATCGAATTTGTCCCGATCGAGAAGCTIGEKEYCILEDVEALT CTTTGAGAGAGTGGACTATAGAATAGGCGAGAAAGAATACTGCATCLDNRDKLIWKKVPYVM CTTGAGGACGTTGAGGCGCTGACTCTTGACAACAGAGACAAACTTARHRAKKKVYRIWITNS TTTGGAAGAAGGTGCCCTACGTCATGCGTCACAGGGCAAAGAAAAAWYIDVTEDHSLIVAED GGTCTACCGTATCTGGATTACTAATTCATGGTACATAGACGTTACAGLKEARPMEIEGKSLI GAGGACCACTCCCTGATTGTGGCTGAGGACGGGCTGAAGGAGGCCCATKDDLSGVEYIKPHA GCCCCATGGAAATTGAGGGCAAGTCTCTGATTGCAACTAAAGATGAIEEISYNGYVYDIEVE TCTCTCTGGCGTTGAGTACATCAAGCCTCACGCTATTGAGGAGATTGTHRFFANGILVHNTY AGTTACAACGGTTACGTGTACGATATCGAAGTGGAGGGTACTCATADIYRTTRVNQPSIVGT GATTCTTCGCTAATGGGATACTGGTGCATAACACCTACGACATCTAATFDQYWSVRTSKRTS TAGGACGACACGCGTCAACCAGCCTTCCATTGTGGGGACAGCCACGGTVTVTDHFRAWANRG TTCGATCAGTACTGGAGCGTGCGCACCTCTAAGCGGACTTCAGGAALNLGTIDQITLCVEGY CAGTGACCGTGACCGATCACTTCCGCGCCTGGGCGAACCGGGGCCTQSSGSANITQNTFSQG GAACCTCGGCACAATAGACCAAATTACATTGTGCGTGGAGGGTTACSSSGSSGGSSGSTTTT CAAAGCTCTGGATCAGCCAACATCACCCAGAACACCTTCTCTCAGGRIECENMSLSGPYVSR GCTCTTCTTCCGGCAGTTCGGGTGGCTCATCCGGCTCCACAACGACITNPFNGIALYANGDT TACTCGCATCGAGTGTGAGAACATGTCCTTGTCCGGACCCTACGTTARATVNFPASRNYNFR AGCAGGATCACCAATCCCTTTAATGGTATTGCGCTGTACGCCAACGLRGCGNNNNLARVDLR GAGACACAGCCCGCGCTACCGTTAACTTCCCCGCAAGTCGCAACTAIDGRTVGTFYYQGTYP CAATTTCCGCCTGCGGGGTTGCGGCAACAACAATAATCTTGCCCGTWEAPIDNVYVSAGSHT GTGGACCTGAGGATCGACGGACGGACCGTCGGGACCTTTTATTACCVEITVTADNGTWDVYA AGGGCACATACCCCTGGGAGGCCCCAATTGACAATGTTTATGTCAGDYLVIQSEKDEL* TGCGGGGAGTCATACAGTCGAAATCACTGTTACTGCGGATAACGGC (SEQ ID NO:115) ACATGGGACGTGTATGCCGACTACCTGGTGATACAGAGCGAGAAGG ACGAGCTGTGA (SEQ IDNO: 187)

Example 6—Plant Transformation

Maize Transformation

Agrobacterium-mediated transformation of immature maize embryos wasperformed as described in Negrotto et al., (2000) Plant Cell Reports 19:798-803, which is incorporated herein by reference as if fully setforth. Transformation plasmids and selectable marker genes used fortransformation were cloned into a pAG-series vector suitable for monocottransformation as described above. The vectors utilized for this examplecontained the phosphomannose isomerase (PMI) gene (Negrotto et at (2000)Plant Cell Reports 19: 798-803) as a selectable marker, but othermarkers could be used in the same capacity.

Transformation Vector and Agrobacterium Strains

Agrobacterium tumefaciens transformation vectors were constructed usingstandard molecular techniques known in the art, as described above. Theplasmids were introduced into Agrobacterium strains LBA4404+pSB1 (Ishidaet al. (1996) Nature Biotechnology 14:745-750, which is incorporatedherein by reference as if fully set forth).

Overnight cultures of the Agrobacterium strain containing the plasmidwere grown for two days on plates with solid YP medium for 2 to 4 daysat 28° C. containing 100 mg/L spectinomycin and 10 mg/L tetracycline.

Agrobacterium was re-suspended in LS-inf media supplemented with 100 mMacetosyringone (As) (LSAs medium) (Negrotto et al., (2000) Plant CellRep 19: 798-803, which is incorporated herein by reference as if fullyset forth) until the Agrobacterium cells were uniformly dispersed in thesuspension. The Agrobacterium suspension was then diluted to an OD₆₆₀ inthe range of 0.5 to 0.8 and vortexed for about 15 seconds.

Infection and Co-Cultivation of Maize Immature Embryos

Maize (Zea maize cultivars HiII, A188 or B73) stock plants were grown ina greenhouse under 16 hours of daylight at 28° C. Immature ears werecollected 7 to 15 days after pollination and sterilized by immersing in20% chlorine bleach (available under the registered trademark CHLOROX®)for 15-20 minutes. Sterilized ears were then rinsed thoroughly withsterile water.

Immature zygotic embryos were isolated from the kernels and collectedinto a sterile eppendorf tube containing liquid LS-inf+100 p1M As (LSAs)media. Embryos were vortexed for 5 seconds and rinsed once with freshinfection medium. Infection media was removed, Agrobacterium solutionwas added and embryos were vortexed for 30 seconds and allowed to settlewith the bacteria for about 5 minutes.

After inoculation, immature embryos were then transferred scutellum sideup to LSAs medium and cultured in the dark for two to three days at 22C.

Recovery, Selection of Transformed Maize Embryogenic Tissue and PlantRegeneration

After co-cultivation, immature embryos were transferred onto LSDc mediumsupplemented with 200 mg/l of timentine and 1.6 mg/l silver nitrate(Negrotto et al. 2000). The plates were incubated for 5 to 15 days at28° C. in the dark.

Embryos producing embryogenic callus were transferred to LSD1M0. 5Smedium (LSDc with 5 mg/l Dicamba, 10 g/l mannose, 5 g/l sucrose). Thecultures were selected on this medium for 6 weeks with 3 week subcultureintervals. Surviving cultures were transferred either to LSD1M0.5Smedium to be bulked-up or to Reg1 medium (as described in Negrotto etal., 2000). Following culturing in the light (16 hour light/8 hour darkregiment), green tissues were then transferred to Reg2 medium withoutgrowth regulators (as described in Negrotto et al., 2000) and incubatedfor 1-2 weeks. Well-developed seedlings with leaves and roots weretransferred to Reg3 medium (as described in Negrotto et al., 2000) andgrown in the light.

Leaves were sampled for PCR analysis to identify transgenic plantscontaining the selectable marker gene according to Negrotto et al.(2000), and gene of interest. PCR positive and rooted plants were rinsedwith water to wash off the agar medium, and transplanted to soil andgrown in the greenhouse for seeds.

Switchgrass Transformation

Media used in developing the Agrobacterium-mediated transformationprotocol, employed to transform switchgrass plants, were prepared usingstandard methods known to one of ordinary skill in the art. Thefollowing media were used in the Examples described herein.

Somatic Embryo Induction Medium (SEI)

SEI medium was prepared using 4.3 g of MS basal salt mixture, B5vitamins (100 mg of myo-Inositol, 1 mg of nicotinic acid, 1 mg ofpyridoxine HCl and 10 mg of thiamine HCl), 30 g sucrose, 5 mg 2,4-D and10 mg BAP, 1.2 g/l Gelrite (Sigma, St. Louis, Mo., USA). These reagentswere mixed in sterile water, which was taken up to a final volume of 1L. The pH was adjusted to 5.8 prior to autoclaving.

Regeneration Medium

Regeneration medium was prepared using 4.3 g of MS basal salt mixture,MS vitamins (100 mg of myo-Inositol, 1 mg of nicotinic acid, 1 mg ofpyridoxine HCl and 10 mg of thiamine HCl), 30 g sucrose, and 1.2 gGelrite (Sigma, St. Louis, Mo., USA). These reagents were mixed insterile water and taken up to a final volume of 1 L. The pH was adjustedto 5.8 prior to autoclaving.

Inoculation Medium (SW-1)

SW-1 medium was prepared using 4.3 g MS salts, B5 vitamins (100 mg ofmyo-Inositol, 1 mg of nicotinic acid, 1 mg of pyridoxine HCl and 10 mgof thiamine HCl), 68.5 g sucrose, 36 g glucose, and 1 g casamino acids.These reagents were mixed in sterile water and taken up to a finalvolume of 1 L. The pH was adjusted to 5.8 prior to autoclaving.

Co-Cultivation Medium (SW-2)

SW-2 medium was prepared using 4.3 g MS salts, B5 vitamins (100 mg ofmyo-Inositol, 1 mg of nicotinic acid, 1 mg of pyridoxine HCl and 10 mgof thiamine HCl), 0.7 g L-proline, 10 mg BAP, 5 mg 2,4-D, 0.5 g MES, 20g sucrose, 10 g glucose and 1.2 g Gelrite. These reagents were mixed insterile water and taken up to a final volume of 1 L. The pH was adjustedto 5.8 prior to autoclaving.

Resting Medium (SW-3)

SW-3 medium was prepared using 4.3 g MS salts, B5 vitamins (100 mg ofmyo-Inositol, 1 mg of nicotinic acid, 1 mg of pyridoxine HCl and 10 mgof thiamine HCl), 10 mg BAP, 5 mg 2,4-D, 30 g sucrose and 1.2 g Gelrite.These reagents were mixed in sterile water and taken up to a finalvolume of 1 L. The pH was adjusted to 5.8 prior to autoclaving.

Selection Medium 1 (S1)

51 medium was prepared using 4.3 g MS salts, B5 vitamins (100 mg ofmyo-Inositol, 1 mg of nicotinic acid, 1 mg of pyridoxine HCl and 10 mgof thiamine HCl), 10 mg BAP, 5 mg 2,4-D, 5 g sucrose, 10 g mannose and1.2 g Gelrite. These reagents were mixed in sterile water and taken upto a final volume of 1 L. The pH was adjusted to 5.8 prior toautoclaving. Regeneration medium (R1). R1 medium was prepared using 4.3g MS salts, B5 vitamins (100 mg of myo-Inositol, 1 mg of nicotinic acid,1 mg of pyridoxine HCl and 10 mg of thiamine HCl), 30 g sucrose and 1.2g Gelrite. These reagents were mixed in sterile water and taken up to afinal volume of 1 L. The pH was adjusted to 5.8 prior to autoclaving.

Initiation of Embryogenic Callus Cultures

Mature switchgrass seeds (Panicum virgatum, cv. Alamo) were prepared fortransformation by removing their seed coat using sand paper. With theseed coat removed, individual seeds were selected for sterilization.Switchgrass seeds were sterilized by immersing in 20% chlorine bleach(available under the registered trademark CHLOROX®) for 5-10 minutes.Sterilized seeds were then rinsed thoroughly with sterile water. Sterileseeds were placed onto somatic embryo induction medium (SEI) and wereincubated at 28° C. in the dark for about 3-4 weeks. Resultingembryogenic callus clusters were transferred to fresh SEI medium andcultured for additional 6 weeks with 3 weeks subculture intervals at 28°C. in the dark.

Transformation Vector and Agrobacterium Strains

Agrobacterium tumefaciens transformation vectors were constructed asdescribed above using standard molecular techniques known in the art.The plasmids were introduced into Agrobacterium strains LBA4404+pSB1(Ishida et al. (1996) Nature Biotechnology 14:745-750).

Overnight cultures of the Agrobacterium strain containing the plasmidwere grown for two days on plates with YP medium containing 100 mg/Lspectinomycin and 10 mg/L tetracycline.

Preparation of Agrobacterium for Transformation

Agrobacterium culture was initiated weekly from a glycerol stock storedat −80° C., on YP semi-solid medium containing appropriate antibioticsand grown at 28° C. in an incubator.

The Agrobacterium was streaked onto fresh YP medium containingappropriate antibiotics the day before the inoculation and was grown ina 28° C. incubator. For plant transformation use, the Agrobacterium wascollected from the plate using a disposable plastic inoculation loop andsuspended in liquid inoculation medium, such as SW1, in a sterile 15 mldisposable polypropylene centrifugation tube. Agrobacterium wasresuspended in the tube by vortexing for about 3 to 5 minutes until theAgrobacterium cells were uniformly dispersed in the suspension. TheAgrobacterium suspension was then diluted to an OD₆₆₀ in the range of0.5 to 0.8 and vortexed for about 15 seconds.

Infection and Co-Cultivation of Switchgrass Embryogenic Callus Cultures

The switchgrass type II repetitive somatic embryogenic callus clusters,2 mm to 3 mm in diameter, were infected with Agrobacterium by mixing theexplants with bacterial suspension as prepared above, and vortexed for30 sec. The mixture was incubated with the prepared explants for about 3to 15 minutes at room temperature.

Following infection, the Agrobacterium suspension explants were placedon co-cultivation medium (SW-2) in 100×15 mm Petri plates and wereincubated for 2 to 3 days at 22° C. in the dark.

Regeneration and Selection of Transgenic Plants

After co-cultivation, the explants were transferred onto recovery mediumwith antibiotics to kill Agrobacterium or to inhibit Agrobacteriumgrowth, without selection agent, such as recovery medium (SW3)supplemented with 200 mg/L timentin. The plates were incubated for 5 to15 days at 28° C. in the dark. The explants were then transferred to S1solid medium (10 g/L mannose and 5 g/l sucrose) supplemented withantibiotics for about 14 to 21 days. The explants were then transferredto fresh S1 medium (10 g/L mannose and 5 g/l sucrose) for about 14 to 21days. Resistant clones were transferred to embryo differentiation mediumR1 (5 g/l mannose and 10 g/l sucrose) and were incubated at 28° C. inthe dark for about 2 to 3 weeks.

Differentiating plant tissues were transferred to fresh embryodifferentiation medium R1 (5 g/l mannose and 10 g/l sucrose) and wereincubated at 26° C. in the light for about 2 to 3 weeks.

Well-developed seedlings with leaves and roots were transferred torooting medium. Leaves were sampled for PCR analysis to identifytransgenic plants containing the selectable marker gene according toNegrotto et al. (2000), and gene of interest. PCR positive and rootedplants were rinsed with water to wash off the agar medium, andtransplanted to soil and grown in the greenhouse for seeds.

Sorghum Somatic Embryogenic Culture Transformation

Materials and Methods

Media used in the Agrobacterium-mediated transformation protocolemployed to develop transformed sorghum plants were prepared usingstandard methods known to one of ordinary skill in the art. Thefollowing media were used in the Examples described herein.

Somatic Embryo Induction Medium (SGWT-SEI)

4.3 g of MS basal salt mixture, B5 vitamins (100 mg of myo-Inositol, 1mg of nicotinic acid, 1 mg of pyridoxine HCl and 10 mg of thiamine HCl),1.2 g KH₂PO₄, 2.0 g L-proline, 0.9 g L-asparagine, 30 g sucrose, 1.5 mg2,4-D, and 8 g Agar (Sigma, St. Louis, Mo., USA) were combined insterile water. The final volume of the mixture was taken up to 1 L usingsterile water. The pH was adjusted to 5.8 prior to autoclaving.

Regeneration Medium (SGWT-R)

4.3 g of MS basal salt mixture, B5 vitamins (100 mg of myo-Inositol, 1mg of nicotinic acid, 1 mg of pyridoxine HCl and 10 mg of thiamine HCl),1.2 g KH₂PO₄, 2.0 g L-proline, 0.9 g L-asparagine, 30 g sucrose, 1.0 mgIAA, 0.5 mg kinetin and 2.4 g Gelrite (Sigma, St. Louis, Mo., USA) werecombined in sterile water. The final volume of the mixture was taken upto 1 L using sterile water. The pH was adjusted to 5.8 prior toautoclaving.

Inoculation Medium (SGI-1)

4.3 g MS salts, B5 vitamins (100 mg of myo-Inositol, 1 mg of nicotinicacid, 1 mg of pyridoxine HCl and 10 mg of thiamine HCl), 68.5 g sucrose,36 g glucose, 1.0 g casamino acids, and 1.5 mg 2,4-D were combined insterile water. The mixture was taken up to a final volume of 1 L usingsterile water. The pH was adjusted to 5.2 prior to autoclaving.

Co-Cultivation Medium (SGC-2)

4.3 g of MS basal salt mixture, B5 vitamins (100 mg of myo-Inositol, 1mg of nicotinic acid, 1 mg of pyridoxine HCl and 10 mg of thiamine HCl),1.2 g KH₂PO₄, 2.0 g L-proline, 0.9 g L-asparagine, 20 g sucrose, 10 gglucose, 0.5 g MES, 1.5 mg 2,4-D, 40 mg acetosyringone, and 8 g agarwere combined in sterile water. The mixture was taken up to a finalvolume of 1 L using sterile water. The pH was adjusted to 5.8.

Somatic Embryo Induction Medium (SGCI-3)

4.3 g of MS basal salt mixture, B5 vitamins (100 mg of myo-Inositol, 1mg of nicotinic acid, 1 mg of pyridoxine HCl and 10 mg of thiamine HCl),1.2 g KH₂PO₄, 2.0 g L-proline, 0.9 g L-asparagine, 30 g sucrose, 1.5 mg2,4-D, and 8 g agar (Sigma, St. Louis, Mo., USA) were combined insterile water. The mixture was taken up to a final volume of 1 L usingsterile water. The pH was adjusted to 5.8. After autoclaving timentinwas added to the final concentration of 200 mg/l.

Selection Medium 1 (SGS1-4)

4.3 g of MS basal salt mixture, B5 vitamins (100 mg of myo-Inositol, 1mg of nicotinic acid, 1 mg of pyridoxine HCl and 10 mg of thiamine HCl),1.2 g KH₂PO₄, 2.0 g L-proline, 0.9 g L-asparagine, 5 g sucrose, 10 gmannose, 1.5 mg 2,4-D, and 8 g agar (Sigma, St. Louis, Mo., USA) werecombined in sterile water. The mixture was taken up to a final volume of1 L using sterile water. The pH was adjusted to 5.8. After autoclavingtimentin was added to the final concentration of 200 mg/l.

Selection Medium 2 (SGS2-5)

4.3 g of MS basal salt mixture, B5 vitamins (100 mg of myo-Inositol, 1mg of nicotinic acid, 1 mg of pyridoxine HCl and 10 mg of thiamine HCl),1.2 g KH₂PO₄, 2.0 g L-proline, 0.9 g L-asparagine, 5 g sucrose, 9.0 gmannose, 1.5 mg 2,4-D, and 8 g agar (Sigma, St. Louis, Mo., USA) werecombined in sterile water. The mixture was taken up to a final volume of1 L using sterile water. The pH was adjusted to 5.8. After autoclavingtimentin was added to the final concentration of 200 mg/l.

Regeneration Medium (SGR1-6)

4.3 g of MS basal salt mixture, B5 vitamins (100 mg of myo-Inositol, 1mg of nicotinic acid, 1 mg of pyridoxine HCl and 10 mg of thiamine HCl),1.2 g KH₂PO₄, 2.0 g L-proline, 0.9 g L-asparagine, 20 g sucrose, 5.0 gmannose, 1.0 mg IAA, 0.5 mg kinetin and 2.4 g Gelrite (Sigma, St. Louis,Mo., USA) were combined in sterile water. The mixture was taken up to afinal volume of 1 L using sterile water. After autoclaving timentin wasadded to the final concentration of 200 mg/l.

Initiation of Somatic Embryogenic Cultures from Immature Zygotic Embryos

Sorghum (Sorghum bicolor (L.) Moench) immature caryopses were sterilizedby immersing in 20% chlorine bleach (CHLOROX®) for 20 minutes.Sterilized caryopses were then rinsed thoroughly with sterile water.

Immature embryos were isolated from caryopses and were placed ontosomatic embryo induction medium (SGWT-SEI). Plates were incubated at 26to 28° C. in the dark for about 2 to 4 weeks. The resulting somaticembryogenic clusters were used for transformation experiments ortransferred to fresh SEI medium and cultured for additional 3 to 6 weekswith 3 weeks subculture intervals at 28° C. in the dark prior to use intransformation experiments.

Transformation Vector and Agrobacterium Strains

Agrobacterium tumefaciens transformation vectors were constructed asdescribed above using standard molecular techniques known in the art.The plasmids were introduced into Agrobacterium strains LBA4404+pSB1(Ishida et al. (1996) Nature Biotechnology 14:745-750).

Overnight cultures of the Agrobacterium strain containing the plasmidwere grown for two days on plates with YP medium containing 100 mg/Lspectinomycin and 10 mg/L tetracycline.

Preparation of Agrobacterium for Transformation

Agrobacterium culture was initiated weekly from glycerol stocks, storedat −80° C., onto YP semi-solid medium containing appropriate antibioticsand grown at 28° C. in an incubator.

The Agrobacterium was streaked onto fresh YP medium containingappropriate antibiotics the day before the inoculation and was grown ina 28° C. incubator. For plant transformation use, the Agrobacterium wascollected from the plate using a disposable plastic inoculation loop andsuspended in liquid inoculation medium, such as SW1, in a sterile 15 mldisposable polypropylene centrifugation tube. Agrobacterium wasresuspended in the tube by vortexing for about 3 to 5 minutes until theAgrobacterium cells were uniformly dispersed in the suspension. TheAgrobacterium suspension was then diluted to an OD660 of 0.5 to 0.8 andvortexed for about 15 seconds.

Infection and Co-Cultivation of Sorghum Somatic Embryogenic Cultures

The sorghum somatic embryogenic clusters were infected withAgrobacterium by mixing the explants with bacterial suspension preparedas described above, and vortexed for 30 sec. The mixture was incubatedwith the prepared explants for about 3 to 15 minutes at roomtemperature.

Following infection, the Agrobacterium suspension explants were placedon co-cultivation medium (SGC-2) in 100×15 mm Petri plates and wereincubated for 2 to 3 days at 22° C. in the dark.

Regeneration and Selection of Transgenic Plants

After co-cultivation, the explants were transferred onto recovery mediumwith antibiotics to kill Agrobacterium, or to inhibit Agrobacteriumgrowth, without a plant selection agent, such as recovery medium(SGCI-3) supplemented with 200 mg/L timentin. The plates were incubatedfor 5 to 15 days at 28° C. in the dark.

The explants were then transferred to SGS1-4 solid medium (10 g/Lmannose and 5 g/l sucrose) supplemented with antibiotics for about 14 to21 days.

The explants were then transferred to fresh SGS2-5 medium (10 g/Lmannose and 5 g/l sucrose) for about 14 to 21 days.

Resistant clones were transferred to embryo differentiation mediumSGR1-6 (5 g/l mannose and 10 g/l sucrose) and were incubated at 28° C.in the dark for about 2 to 3 weeks.

Differentiating plant tissues were transferred to fresh embryodifferentiation medium R1 (5 g/l mannose and 10 g/l sucrose) and wereincubated at 26° C. in the light for about 2 to 3 weeks.

Well developed seedlings with leaves and roots were transferred torooting medium.

Leaves were sampled for PCR analysis to identify transgenic plantscontaining the selectable marker gene according to Negrotto et al.(2000), and gene of interest. PCR positive and rooted plants were rinsedwith water to wash off the agar medium, and transplanted to soil andgrown in the greenhouse for seeds.

Example 7—Analysis of Transgenic Plants

Microbial Production of Enzymes

As part of the analysis of transgenic plants, microbial production canbe utilized to generate enzyme standards. Although the microbiallyproduced enzymes may have different glycosylation patterns, or otherpost-translational modifications, than the protein expressed in plants,the microbial protein is an acceptable standard for generatingantibodies, for assay measurements, and for western blots.

Example 8—Production of Xylanases Using P. pastoris

Genes encoding enzymes of interest were cloned into expression vectorsand transformed into suitable expression hosts. Pichai pastorisexpression was performed in YPD media at 30° C. and 300 rpm. Culturesupernatants were harvested after three to five days of expressioncorresponding to the time point of highest enzyme activity per ml ofclarified supernatant. The supernatant was concentrated by tangentialflow filtration with a 10 kDa MWCO membrane and exhaustively bufferedexchanged with appropriate reaction buffer.

The amount of enzyme present in the concentrated culture supernatantswas determined by treating a 10 μl sample with PNGaseF (NEB) accordingto the manufacture's protocol to remove N-linked glycans from the targetprotein. The sample was serially diluted and 10 μl of each dilution wasfractionated by SDS-PAGE and stained with Simply Blue Safe stain(Invitrogen) according to the manufacture's guidelines. Theconcentration of the sample was designated as the highest dilutionfactor in which the target protein was still detectable after staining.

Rabbit Antiserum Generation

Antibodies that cross react with specific proteins were generated by NewEngland Peptide. Proteins of interest were expressed in Pichia pastoris.The resulting culture supernatant was concentrated by tangential flowfiltration using a 10 kDa MWCO filter (Millipore) and in some casefurther purified by column chromatography. The sample concentrate wasfurther polished using centricon filtration device with a 10 kDa MWCO(Millipore) then fractionated by SDS-PAGE. The protein bandcorresponding to the predicted molecular weight of the target proteinwas excised from the gel using a razor blade and sent to New EnglandPeptide for anti-sera generation. Upon receipt, the specificity of eachantiserum was validated by Western Blot, aliquoted and stored at 4° C.or −20° C. Western Blot analysis was performed under standard conditionsknown in the art.

Example 9—Determination of Xylanase Activity by Reducing SugarMeasurement

Xylanase activity was determined using birch wood xylan as a substrateand measuring the production of reducing sugar ends with theNelson-Somogyi reducing sugar microassay (Green et al. 1989, Adaptationof the Nelson-Somogyi reducing-sugar assay to a microassay usingmicrotiter plates, Anal Biochem. 1989 Nov. 1; 182(2):197-9, which isincorporated by reference herein as if fully set forth). A 2% (w/v)substrate solution was prepared by dissolving birchwood xylan (Sigma) inboiling water. 0.02% azide (final concentration) was added as apreservative. Reagents for the Nelson-Somogyi reducing sugar assay wereprepared as previously described (Green et al. 1989). Proteinconcentrations were determined using the BCA protein assay kit (ThermoScientific) or represented as a dilution factor as described above.

Assays consisted of 250 μl of 2% birchwood xylan, 250 μl buffer, andvarying volumes of xylanase preparation (or xylanase standards used togenerate a standard curve) in a total reaction volume of one milliliter.Assays were conducted at 60° C. for 20 minutes then placed on ice tostop the reaction. From each reaction, 50 μl of each reaction wereassayed for the presence of reducing sugars using the Nelson-Somogyireducing sugar assay as previously described. Xylanase activity unitswere determined from results corresponding to the linear range of theanalysis. The specific activity of the enzyme preparations wascalculated by the following equation: Specific Activity=(mM reducingends produced)/(dilution factor concentration).

Referring to FIG. 7, the specific activity of three xylanases withaccession numbers P40942, P77853 and O30700 was identified. As shown,the specific activity of O30700 is 5 times that of P40942 and P77853when birchwood xylan is used as a substrate.

Example 10—Analysis of Transgenic Plant Material

Transgenic plants were assayed to determine the levels of accumulatedactive enzyme. For these assays, samples of liquid nitrogen frozen leaftissue were ground in a mortar and pestle and the grindate collected. 10mg of frozen leaf grindate was distributed into each well of amicrotiter. To each well 200 μl of 100 mM buffer was added and thereactions mixed by pipetting. The plates were sealed and placed into ashaking incubator (200 rpm) at 55° C. for 16 hours. Post incubation,each reaction was applied to a Multiscreen HTS filterplate with a 1.2 μmglass fiber filter (Millipore, Billerica Mass.) and filtered bycentrifugation at 500×g for 3 minutes. Enzyme activity was assessed byassaying 50 μl of the resulting filtrate using the Nelson-Somogyireducing sugar assay as previously described. Extracted protein wasdetermined using the BCA protein assay kit (Thermo). Levels of activitywere presented as mM reducing sugar ends produced per mg of extractedprotein.

Referring to FIG. 8, the activity of various transgenic plant samplesexpressing Xylanase P77853 is shown. The samples labeled AG2014 andAG2015 were transformed with the plasmids pAG2014 and pAG2015,respectively, and AG2004 is a control. The production of reducing sugarsin transgenic plant samples as compared to the wildtype sample indicatedthe accumulation of active xylanase in transgenic plant tissue.

Example 11—Determination of Activity Against pNP-Conjugated Glycosides

In order to characterize the enzymatic range of activities of particularxylanases several assays were performed using p-nitrophenol(pNP)-conjugated glycosides. One molar stocks of the substrates weremade in dimethylsulfoxide. Reactions consisted of 5 mM (finalconcentration) substrate, 100 mM buffer in 50 μl and 1-10 μl of enzymepreparation. Reactions were prepared then incubated at 60° C. for onehour. The reactions were stopped and developed upon addition of 100 μlof 0.1M carbonate buffer pH 10.5. Hydrolysis of the substrate, which wasindicated by the formation of pNP, was detected as an increase ofabsorbance at 400 nm.

Polysaccharide endohydrolysis substrates was also determined using AZCLconjugated substrates supplies (Megazyme) and used according to themanufacture's standard protocol. Briefly, 250 μl of a specific bufferwas mixed with 100 μl of enzyme preparation and 150 μl of water. Thereaction was placed in a water bath incubator set at the desiredtemperature (usually between 37° C. and 70° C.) for five minutes afterwhich one tablet of either xylazyme AX or cellazyme C was added. Thereaction were incubated for 10 minutes then removed from the incubatorand stopped with 10 ml of 2% (w/v) Tris Base (Sigma®). Endohydrolysis ofthe polyscaahride substrate was indicated by the release of soluble bluedye. The amount of released dye was quantified by measuring absorbanceof the reaction supernatant at 590 nm. Controls for these reactionsinclude protein extracts from the P. pastoris or E. coli wild typestrain and recombinant enzyme producing strain.

Table 1, below, demonstrates the detected activities of severalxylanases. As indicated endo-xylanase activity was detected for theP77853, O30700 and P40942 samples. Cellobiohydrolase and β-glucosidaseactivities were detected in samples contain P40942 indicating that thisenzyme is capable of endohydrolysis of xylan and exohydrolysis ofcellulose and cellobiose.

TABLE 1 β-xylo- Cellobio- β-gluco- Sample Xylanase sidase Cellulasehydrolase sidase P77853 + − − − − O30700 + − − − − P40942 + − − + + P.pastoris − − − − − E. coli − − − − −

Example 12—Determination of Thermal Stability

Thermal stability of enzymes was assessed by recovery of enzymaticactivity after incubation at elevated temperatures. Briefly,preparations of xylanase P77, 030 or 040 were incubated at 4° C., 50°C., 60° C., 70° C. or 80° C. for one hour then assayed using thexylazyme AX substrate as described above. Referring to FIG. 9, xylanasesO30700 and P77853 retain nearly 100% activity after one hour incubationat up to 60° C., but have reduced activity when exposed to 70° C. and80° C. temperature treatments. Xylanase P40942 retains nearly 100%activity after one hour at temperatures up to 70° C., while at 80° C.its activity was reduced relative to exposure at the lower temperaturestested.

The thermal stability of the enzyme is one characteristic that mayimpact its utility in different applications. For example, in processinglignocellulosic bioimass; e.g., that derived from corn (stover),switchgrass, miscanthus, sorghum, or sugarcane, if the transgenicbiomass material is to be treated at 70° C. for one hour, P40942 may bea better enzyme to deliver xylanase activity than O30700 or P77853because of its increased stability at that temperature. In contrast, iftransgenic grain; e.g., from transgenic corn or sorghum, is going to beused in formulating an animal feed ration, where the feed is ground andmixed at a temperature of 50° C., then any of these enzymes may besufficiently thermal stable. However, these uses of particular enzymesdo not preclude other uses of the same particular enzymes.

Example 13—Materials and Methods for Evaluating Transgenic Plants andtheir Pretreatment and Enzymatic Hydrolysis Processes

Various process configurations may be used to process biomass andcertain plant tissues. One process configuration is referred to as amacro-scale process, which can be scaled up, and is described directlybelow. Another process configuration is referred to as a micro-scaleprocess, which can be used for plant evaluation, and is detailed below,following the description of a macro-scale process.

Example 13a—Macro-Scale Process

Macro-scale sequential low temperature chemi-mechanical pretreatment(CMPT) and one-stage enzymatic hydrolysis:

Referring to FIG. 10, biomass conversion to fermentable sugars by amacro-scale process method was used with several feedstocks. FIG. 10illustrates the process flow diagram for the macro-scale process.

Biomass Substrate Preparation:

Corn stover was transformed with the noted plasmid containing either aβ-glucosidase, endoglucanase, cellobiohydrolase, FAE, or xylanase, orcombination of enzymes. The vector used may be any vector encoding aCWDE or derivative thereof, including any one or more of the vectorsdisclosed herein. In this example, the vector was pAG2015, pAG2042, andpAG2063. The stover was dried in an air-circulator at 37° C. for about 2weeks. The dried corn stover 1010 was cut to 1.0-1.5 inch long.

Pretreatment:

The cut dried corn stover 1010 was pretreated at step 1020 by usingeither pure water or a combination of 8%-38% (wt./wt. on corn stover)ammonium bisulfite and 4%-19% (wt./wt. on corn stover) ammoniumcarbonate (pH 7.6-8.5). The biomass was added to a flask withpretreatment solution at a liquid-to-solid (L/S) ratio of 8. The mixturewas shaken at temperatures of 40° C.-90° C. for four to 19 hours. Thepretreated material was filtered using VWR grade 415 filter paper, andthe material 1025 was collected for further analysis.

Refining:

The pretreated biomass was refined at step 1030 in a blend with DI waterat 40° C.-90° C. After blending, the biomass was filtered using VWRgrade 415 filter paper. The refined biomass (pulp) that did not passthrough was washed with DI water at 40° C.-90° C. DI water. The pulp1035 was stored at 4° C. for moisture balance and further enzymatichydrolysis.

Enzymes:

Accellerase™ 1000 enzyme (Genencor International, Rochester, N.Y.), wasused. The endoglucanase activity was 2500 CMC U/g (minimum). Thebeta-glucosidase activity was 400 pNPG U/g (minimum). The appearance wasbrown liquid. The pH was 4.8-5.2.

Alternatively, a cocktail of enzymes were used, which contained:Endoglucanase (C8546), β-glucosidase (49291), and xylanase (X2753) allpurchased from Sigma (St. Louis, Mo.), and a cellobiohydrolase (E-CBHI)that was purchased from Megazyme (Wicklow, Ireland).

Enzymatic Hydrolysis:

The NREL standard protocol (LAP-009) was followed. At step 1040, thepretreated and refined stover was hydrolyzed in 0.1 M sodium citrate (pH5.0) at a biomass solid content of 6.0% at an enzyme loading of 0.2-0.4ml per g corn stover to release sugar 1045. The reaction occurred in a250 mL erhlenmeyer flask at 250 rpm for 0-48 hr period at 45° C.-55° C.Depending upon the enzyme mixture and enzyme expressed in the plant, thepH was varied from 5 to 9. The preferred pH for these enzyme mixtureswas usually 5.

Tetracycline or an equivalent antibiotic may optionally be added to thehydrolysis to prevent the growth of any potential microbialcontamination.

Analysis of Fermentable Sugars:

The hydrolysate samples were heated at 95° C. for 20 min and thencentrifuged at 9,000×g, following which the supernatants were clarifiedby passage through 0.20 μm PVDF filters (Cat.#: 09-910-13, FisherScientific, Pittsburgh, Pa.). Monosaccharide and disaccharideconcentrations were determined by high performance liquid chromatography(HPLC), using a Shimadzu LC-20 AD binary pump with LC solutions software(Shimadzu, Kyoto, Japan). Sugar concentrations were determined using anAminex HPX-87P sugar column (Bio-Rad Laboratories, Hercules, Calif.)operating at 0.6 ml/min and 85° C. with degassed water as the mobilephase. Peak areas for all samples, analyzed with an RI detector (RIDLOAD), were integrated and the values were compared to standard curvesfor quantification.

Results of Macro-Scale Processing

1—Corn Stover from Wild Type AxB Plants.

For corn stover, the theoretical yield of sugar is 33.5% (wt/wt) glucoseand 16.3% (wt/wt) xylose.

Pretreatment: conducted as described above with either 8% ammoniumbisulfate and 4% ammonium carbonate or 38% ammonium bisulfate, 19%ammonium carbonate at a temperature of 70° C. for 4 hrs.

Enzyme hydrolysis: conducted as described above for 24 or 48 hrs.

The results are presented in Table 2, below. 54.5% (24 hours) and 62.3%(48 hours) glucose recovery yields as well as 20% (24 hours) and 27.5%(48 hours) of xylose recovery yields can be achieved in one-day andtwo-day enzymatic hydrolysis from diluted chemical pretreatment. Theresults demonstrate the efficiency of low temperature CMPT on enzymatichydrolysis.

TABLE 2 Glucose and xylose yields from enzymatic hydrolysis ofpretreated corn stover (A × B) 8% Bisulfite, 38% Bisulfite, Pretreatment4% Carbonate 19% Carbonate Enzymatic hydrolysis 24 48 24 48 time (hrs)Glucose (g/100 g stover) 18.292 21.056 23.995 24.300 Xylose (g/100 gstover) 3.285 4.483 5.637 5.836

2—Stover.

Oven-dried, wild type AxB corn stover was tested and compared against amixture of stover from nine pAG2015 transgenic corn plants (referred toin this example as “2015M”).

Pretreatment: conducted as described above with 16% ammonium bisulfateand 8% ammonium carbonate (pH 7.6) at 70° C. for 4 hrs.

Enzyme hydrolysis: conducted as described above for 0 or 24 hrs.

The results are presented in Table 3, below. Better hydrolysisperformance in terms of sugar yields were observed from the pAG2015transgenic corn plants than wild-type AxB plants.

TABLE 3 Glucose and xylose yields from enzymatic hydrolysis ofpretreated corn stover (A × B and 2015M). Plant source 2015M Wild type A× B Enzymatic Hydrolysis (hrs) 0 24 0 24 Glucose (g/100 g stover) 2.17118.332 2.232 14.771 Xylose (g/100 g stover) 0.390 3.797 0.380 3.303

Example 13b—Micro-Scale Process: Simplified Low TemperatureChemi-Mechanical Pretreatment (CMPT) and Enzymatic Hydrolysis

Referring to FIG. 11, a micro-scale saccharification method was used toscreen several biomass feedstocks for conversion to fermentable sugarsusing either a one-stage or two-stage enzymatic hydrolysis.

Biomass Substrate Preparation:

Corn stover 1110 from corn transformed with the desired vectorcontaining either a beta-glucosidase, endoglucanase, cellobiohydrolase,FAE, or xylanase, or combination of enzymes was obtained. The stover wasdried in an air-circulator at 37° C. for about 2 weeks. After drying,the corn stover was cut to 1.0-1.5 inch long. The stover was milled atstep 1120 using UDY mill (Model 014, UDY Corporation, Fort Collins, Co)with a screen of 0.5 mm.

Pretreatment:

The milled corn stover was pretreated at step 1130 by using either purewater or chemicals. The biomass was added to 2-mL tubes withpretreatment solution at a liquid-to-solid ratio of 10. 20 mg of biomasscould be utilized. The mixture was shaken at temperature of 40° C.-90°C. for 15-19 hrs. The pre-treated material was subject to enzymatichydrolysis without inter-stage washing.

Enzymes:

Endoglucanase (C8546), beta-glucosidase (49291), and xylanase (X2753)were all purchased from Sigma® (St. Louis, Mo.). The cellobiohydrolase(E-CBHI) was purchased from Megazyme® (Wicklow, Ireland).

Enzymatic Hydrolysis:

The process is based on the NREL standard protocol (LAP-009).

One-Stage Hydrolysis:

The milled, pretreated stover was suspended at a 2% (w/v) glucan loadingin polybuffer (50 mM Na citrate, 20 mM K-phosphate, dibasic, 17 mMarginine, 40 mM glycine, 25 mM EPPS, 20 mM HEPES, 0.02% sodium azide)with pH values ranging from 3.5 to 5.0. The pH used was based on finalpH of the suspended pretreated stover. The cocktail enzyme loading wasbased on experiments using 10 mg stover and are given in Table 4, below.Analysis was done on the biomass without any added enzymes(no-cocktail), and with the cocktail missing the xylanse, endoglucanase,or other enzymes that were expressed in the plant (cocktail minusxylanase or endoglucanase depending on the enzyme expressed in plants),in the hydrolysis. This was done to evaluate the effect of the in plantaexpressed enzymes on hydrolysis. Samples were hydrolyzed at 40° C. or50° C. for 48-96 hrs at 200 rpm (1 mL reaction volume).

Tetracycline or an equivalent antibiotic may optionally be added to thehydrolysis to prevent the growth of any potential microbialcontamination.

TABLE 4 Enzyme loading for a full cocktail Enzyme Enzyme loading per 10mg stover endoglucanase 0.5 μM cellobiohydrolase 0.1 μM β-glycosidase0.01 μM  endoxylanase 0.3 μM

Two-Stage Hydrolysis:

The first-stage enzymatic hydrolysis was named depending on the enzymesexpressed in plant (for example, “xylanase hydrolysis” or “glucanasehydrolysis”). The second-stage enzymatic hydrolysis that followed wasnamed “enzyme cocktail hydrolysis.”

For the first-stage, milled, pretreated stover was suspended at a 3%(w/v) glucan loading in polybuffer with pH's ranging from 5.0 to 8.4.The pH used was based on the optimal pH for the plant expressed enzyme.This hydrolysis was conducted at 55° C., 300 rpm for 24-48 hrs.

For the enzyme cocktail hydrolysis, the pH was adjusted to 5.0 usingconcentrated HCl as needed. Then cocktail enzymes were added to samplesas noted in one-stage enzymatic hydrolysis, resulting in samples with nococktail, the full cocktail, and the cocktail minus xylanase orendo-glucanase. Polybuffer pH 5.0 was added for a final solid content of2%. Samples were hydrolyzed at 50° C. at 200 rpm for 48-96 hrs.

Tetracycline or an equivalent antibiotic may optionally be added to thehydrolysis to prevent the growth of any potential microbialcontamination.

Analysis of Fermentable Sugar:

The hydrolysate samples were incubated at 95° C. for 20 min and thencentrifuged at 9,000×g, following which the supernatants were clarifiedby passage through 0.20 μm PVDF filters. Monosaccharide and disaccharideconcentrations were determined by high performance liquid chromatography(HPLC), using a Shimadzu LC-20 AD binary pump with LC solutions software(Shimadzu, Kyoto, Japan). Sugar concentrations were determined using anAminex HPX-87P sugar column (Bio-Rad Laboratories, Hercules, Calif.)operating at 0.6 ml/min and 85° C. with degassed water as the mobilephase. Peak areas for all samples, analyzed with an RI detector (RIDLOAD), were integrated and the values were compared to standard curvesfor quantification.

Results of Micro-Scale Processing

1—One-Stage Enzymatic Hydrolysis, pAG2015.

Plant stover analyzed: a transgenic corn plant designated 2015.05 (madeby transforming corn with pAG2015, which expresses a xylanase) was usedto provide stover. Control plant: a transgenic corn plant designated2004.8.4 (a T1 generation plant, descended from a parent that was madeby transforming corn with pAG2004, which does not encode a xylanaseenzyme) was used to provide control stover. Theoretical sugar yield:2015.05: 33.35% glucose, 18.69% xylose; 2004.8.4: 2015.05: 34.68%glucose, 20.6% xylose.

Pretreatment: conducted as described above with 1:19 (v/v) 15% NH₄OH,20% NH₄Cl at 40° C. or 60° C. for 15 hrs, 300 rpm.

One-stage enzymatic hydrolysis: As described above with 0.02% sodiumazide at 50° C. for 48 hours, 250 rpm.

FIG. 12 illustrates the glucose and xylose yield (percentage on biomassweight) from enzymatic hydrolysis of pretreated corn stover (2015.05 and2004.8.4). As shown in FIG. 12, 2015.05 shows better hydrolysisperformance from both overall hydrolysis yield and based on the effectof the in planta xylanase on hydrolysis (as shown by the “Cocktail-Xyl”treatment). In FIG. 12, the following labels are used: 40 C PT:pretreatment done at 40° C.; 60 C PT: pretreatment at 60° C.“Cocktail-Xyl” denotes the one-stage enzymatic hydrolysis that wasconducted without xylanase in the external enzyme cocktail. Each labeledsample in FIG. 12 shows the results for no cocktail, full cocktail andcocktail-Xyl from left to right.

2—One-Stage Enzymatic Hydrolysis, pAG2063

Plant stover analyzed: transgenic plants designated 2063.13 and 2063.17(made by transforming corn with pAG2063, which expresses a xylanase)were used to provide stover. Control plant designated 2004.8.4 (atransgenic plant made by transforming corn with pAG2004; no xylanaseenzyme expressed) was used to provide control stover.

Pretreatment: conducted as described above with 1:19 (v/v) 15% NH₄OH,20% NH₄Cl, at either 40° C. or 60° C. for 15 hrs, 300 rpm.

One-stage enzymatic hydrolysis: conducted as described above with 1.0mg/ml tetracycline at 50° C. for 48 hours, 250 rpm.

FIG. 13 illustrates the glucose and xylose yield (percentage on biomassweight) from enzymatic hydrolysis of pretreated corn stover (2004.8.4,2063.13, and 2063.17). As shown in FIG. 13, transgenic plant 2063.17shows better hydrolysis performance than control plant and 2063.13 fromboth overall hydrolysis yield and based on the effect of in plantaxylanase on hydrolysis (as shown by the “Cocktail-Xyl” treatment). InFIG. 13, the following labels were used: 40 C PT: pretreatment done at40° C.; 60 C PT: pretreatment at 60° C. “Cocktail-Xyl” denotes theone-stage enzymatic hydrolysis that was conducted without xylanase beingincluded in the external enzyme cocktail. Each labeled sample in FIG. 13shows the results for cocktail-Xyl and full cocktail from right to left.Samples where only two bars are visible show only the cocktail-Xyl andfull cocktail results. Samples where three bars are visible show the nococktail results to the left of the full cocktail results.

3—Two-Stage Enzymatic Hydrolysis, pAG2014.

Plant stover analyzed: transgenic plant 2015.05 was used to providestover; and control plant 2004.8.4 was used to provide control stover.As used herein, a T0 plant is the 1^(st) generation; and a T1 plant isthe 2^(nd) generation, created from the T0 plant seeds.

Pretreatment: conducted as described with DI water at 55° C. for 16 hrs,300 rpm.

First-stage enzymatic hydrolysis (Xylanase hydrolysis): conducted asdescribed previously at 55° C., for 24 hrs with 0.02% sodium azide, 250rpm.

Second-stage hydrolysis (enzyme cocktail hydrolysis): conducted asdescribed at 50° C. using cocktail for 48 hrs.

FIG. 14 illustrates glucose and xylose yields (percentage on biomassweight) from enzymatic hydrolysis of pretreated corn stover (2015.05 and2004.8.4). T0 and T1 2015.05 plants both show better hydrolysisperformance from both overall hydrolysis yield and based on the effectof the in planta xylanase on hydrolysis (See FIG. 14, “Ct-xyl”treatment). In FIG. 14, the following labels were used: “N Ct”: NoCocktail, “F Ct”: Full Cocktail, “Ct-xyl”: Cocktail minus xylanase. Eachlabeled sample in FIG. 14 shows the results for no cocktail, fullcocktail and cocktail-Xyl from left to right.

4—Two-Stage Enzymatic Hydrolysis, pAG2063.

Plant stover analyzed: A transgenic plant designated 2063.17 (made bytransforming corn with pAG2063) was used to provide stover. A controlplant designated 2004.8.4 (made by transforming core with pAG2004) wasused to provide control stover.

Pretreatment: conducted as described with DI water at 55° C. for 16 hrs,300 rpm.

First-stage enzymatic hydrolysis (Xylanase hydrolysis): conducted asdescribed previously at 55° C., for 24 hrs with 0.02% sodium azide, 250rpm.

Second-stage hydrolysis (enzyme cocktail hydrolysis): conducted asdescribed at 50° C. using cocktail for 96 hrs.

FIG. 15 illustrates glucose and xylose yields (percentage on biomassweight) from enzymatic hydrolysis of pretreated corn stover (2064.17 and2004.8.4). As shown in FIG. 15, both glucose and xylose yields for2063.17 are consistently higher than for 2004.8.4 through the course ofpretreatment, 1^(st)-stage xylanase hydrolysis, and 2^(nd) stage enzymecocktail hydrolysis. Xylose yield for 2063.17 increases through thecourses, indicating a positive effect of in planta xylanase on xylanhydrolysis.

In FIG. 15, the following labels were used: PT: levels afterpretreatment; PT-XH: levels after xylanase hydrolysis, 48 hrs: levelsafter 48 hrs of stage two; 96 hrs: levels after 96 hrs of stage two.“Cocktail-Xyl” denotes the one-stage enzymatic hydrolysis that wasconducted without xylanase being included in the external enzymecocktail. The 2004.8.4, PT2004.8.4 PT-XH, 2063.17 and PT2063.17 PT-XHsamples show only no cocktail results. The remaining samples show theresults for no cocktail, full cocktail and cocktail minus xylanase fromleft to right.

5—One-Stage Enzymatic Hydrolysis, pAG2042.

Plant stover analyzed: Transgenic plants designated 2042.2, 2042.3, and2042.6 (made by transforming corn with pAG2042) were used to providestover. Control corn plant 2004.8.4 was used to provide control stover.

Pretreatment: conducted as described above with 0.3 M ammoniumbisulfite/0.34 M ammonium carbonate at temperatures of either 40° C. or60° C. for 19 hrs, 300 rpm.

One-stage enzymatic hydrolysis: conducted as described above with 1.0mg/ml tetracycline at 50° C. for 48 hours, 250 rpm.

FIG. 16 illustrates the glucose yield (percentage on biomass weight)from enzymatic hydrolysis of pretreated corn stover (2042.02, 2042.03,2042.06 and 2004.8.4). As shown in FIG. 16, the glucose yield from2042.3 is significantly higher than the glucose yield from the other twotransgenic plants (2042.2 and 2042.6) as well as control plant(2004.8.4). In FIG. 16, the following labels were used: 40 C PT:pretreatment done at 40° C.; 60 C PT: pretreatment at 60° C. Eachlabeled sample in FIG. 16 shows the results for no cocktail, fullcocktail and cocktail minus endo-glucanase from left to right.

Example 14—Determination of Reducing Sugar Release with Transgenic PlantMaterial

Referring to FIG. 8, transgenic plants were assayed to determine thelevels of accumulated active enzyme. For these assays, samples of liquidnitrogen frozen leaf tissue were ground with a mortar and pestle and theresulting ground samples were collected. 10 mg of frozen leaf grindatewas measured and deposited into a well of a microtiter. To each well,200 μl of 100 mM sodium phosphate buffer (pH 6.5) was added and thereactions mixed by pipetting. The plates were sealed with foil andplaced into a shaking incubator (200 rpm) at 55° C. for 16 hours. Postincubation, each reaction was applied to a Multiscreen HTS filterplatewith a 1.2 μm glass fiber filter (Millipore, Billerica Mass.) andfiltered by centrifugation at 500×g for 3 minutes. Enzyme activity wasassessed by assaying 50 μl of the resulting filtrate using theNelson-Somogyi reducing sugar assay as previously described. Extractedprotein was determined using the BCA protein assay kit (ThermoScientific). Levels of activity were presented as mM reducing sugar endsproduced per mg of extracted protein. The production of reducing sugarsin transgenic plant samples (AG2014 and AG2015) as compared to thenon-xylanase expressing transgenic control plant sample (AG2004)indicated the accumulation of active xylanase in transgenic planttissue.

Example 15—Detection of Autolysis Activity in Transgenic Corn Stover

Ten milligrams (+1-1 mg) of ground sample was applied to a 1.5 mlmicrofuge tube. The ground sample was resuspended in 1 ml of 100 mMsodium phosphate buffer containing 40 μg tetracycline and 30 μg ofcycloheximide. The reactions were incubated for 64 hours at 60° C. withend-over-end mixing (18 rpm). The reaction supernatant was collected andassayed for the presence of reducing sugars using the Nelson-Somogyireducing sugar assay. The results from this assay were reported as mMxylose equivalent reducing ends produced/mg stover by comparison to axylose standard curve.

Example 16—Transgenic Plants and Transgenic Plants Expressing Cell WallDegrading Enzymes

In general, for each transformation vector, at least 20 events weremade. In some case many more (up to 90) transgenic events were made andall events were used to evaluate the effect of the transformationprocess and gene expression.

Transgenic Plants Constructed Using pAG3000 and pAG3001

Referring to FIGS. 17A and 17B, T0 plants were regenerated from thetransformation protocol described above using pAG3000 and pAG3001. Theplant transformation vectors, pAG3000 and pAG3001 were described above.These vectors have the rice actin 1 promoter driving the E. coli genefor phosphomannose isomerase (PMI), which can be used for selectingtransgenic plants or other purposes. The difference between pAG3000 andpAG3001 lies in the junction between the rice actin 1 promoter and thePMI gene. In pAG3000 a partial eukaryotic translation initiation siteconsensus was used, while in pAG3001, the complete eukaryotictranslation initiation site was used. Maize embryos were transformedwith pAG3000 and pAG3001 as described above.

Transgenic plants expressing pAG3000 and pAG3001 were regenerated asdescribed above. Based on experimental results and following theprocedures above, transgenic plants having pAG3000 and pAG3001 wereselected at an average rate of 22.6% and 12.3%, respectively in maize.In other species, transformation efficiency (as defined by the number oftransgenic plants divided by the number targets for transformation,where no more than one transgenic event can be generated per target) isnot easily calculated because target calli are not readily enumerated asdiscrete targets. The maximum efficiency observed in any singleexperiment was 28% for pAG3000 and 14% for pAG3001. Based on these data,using the partial eukaryotic translation initiation site consensussequence provided increased transformation efficiency compared to thecomplete eukaryotic translation initiation sequence. Although the riceactin 1 promoter is considered a relatively strong constitutivepromoter, the transformation efficiencies obtained by linking it to PMIwere unknown and it was uncertain how much better they could be improvedrelative to the CMPS:PMI construct obtained originally. Based on theseresults, the average transformation selection efficiency using CMPS:PMIwas 1.5%, with a maximum of 14%, but efficiencies of 0%, 2%, 3%, 6%, 7%,13%, and 14% were observed in individual experiments. Ranges intransformation efficiency can be impacted by the quality of thetransformation target material, but these averages and ranges helpdefine what could be expected from transformation using theseconstructs. Based on these results, linking PMI to the rice actin 1promoter improved PMI transformation efficiency using the proceduresdescribed above. Furthermore, using the junction between the rice actin1 promoter and PMI in pAG3000, improved the average transformationefficiency above the level of improvement when using the junctionemployed in pAG3001.

As shown in FIGS. 17A and 17B, transgenic plants with pAG3000 (FIG. 17A)and pAG3001 (FIG. 17B) are phenotypically normal for transgenic plantsat this stage of development. The transgenic nature of these plants wasverified using PCR.

Example 17—Transgenic Plants Constructed Using pAG2004 and pAG2005

Referring to FIGS. 18A, 18B, 18C, 19A and 19B, corn was transformed withthe plant transformation vectors, pAG2004 (FIGS. 18A, 18B and 18C) andpAG2005 FIGS. 19A and 19B). These vectors have the rice ubiquitin 3promoter driving the E. coli gene for phosphomannose isomerase (PMI),which can be used for selecting transgenic plants or other purposes. Thedifference between pAG2004 and pAG2005 is that pAG2005 contains anadditional, empty expression cassette that other genes of interest canbe cloned into. In terms of selecting transgenic events, pAG2004 andpAG2005 have the identical rice ubiquitin 3 promoter and PMI selectioncassette. These two vectors combined to give an average transformationefficiency of 20%. In individual experiments, these vectors providedtransformation efficiencies of 0%, 4%, 7%, 10%, 11%, 12%, 13%, 14%, 15%,17%, 18%, 24%, 28%, 29%, 30%, 31%, 32%, 40%, 50%, 53%, and 64%. Rangesin transformation efficiency can be impacted by the quality of thetransformation target material, but these averages and ranges could beexpected from transformation using these constructs.

The rice ubiquitin 3 promoter fused to PMI significantly increasedtransformation efficiencies that were observed relative to CMPS:PMI,using the method described above. Furthermore, the averagetransformation efficiency was greater than that using pAG3001, andsimilar to the efficiency observed using pAG3000. Because the maximumefficiencies obtained using pAG2004 and pAG2005 were greater than thoseobtained using pAG3000, the pAG2004 and pAG2005 selection cassettes wereused for further development of transgenic plants, as described above.

FIGS. 18A, 18B, 18C, 19A and 19B illustrate TO plants regenerated fromthe transformation protocol described above. FIG. 18A shows that anearly senescent pAG2004 transgenic plant is phenotypically normal.FIGS. 18B and 18C show that cobs from a pAG2004 transgenic plant arealso phenotypically normal. FIGS. 19A and 19B show that pAG2005transgenic plants are phenotypically normal. The transgenic nature ofthese plants was verified using PCR.

FIG. 20 illustrates measurement of reducing sugars from transgenic plantevent #15 transformed with pAG2004. In FIG. 20, the buffer samplerepresents the background of the assay, where 1 mg of buffer was used inthe measurement. Because pAG2004 does not express a cell wall degradingenzyme, its reducing sugar measurement represents a negative control tocompare other plants against and is also representative of wild-type,non-transgenic plants.

Example 18—Transgenic Plants Constructed Using pAG2016

The transformation vector pAG2016 was used in transformation toregenerate transgenic plants. This transformation vector was derivedfrom pAG2005 and contains an expression cassette for the production ofbeta-glucoronidase (GUS). In this expression cassette, GUS is fused tothe maize codon optimized PR1a signal peptide, which directs GUS to theapoplast intercellular space. The transformation efficiency of thisvector has an average of 16%, and was in the expected range for the PMIselection cassette used.

Referring to FIGS. 21A and 21B, T0 pAG2016 transgenic plants and cobsare phenotypically normal. The plants were regenerated from thetransformation protocol described above. The transgenic nature of theseplants was verified using PCR. These plants demonstrate that a transgenecan be effectively expressed from the expression cassette containedwithin pAG2005. The transgenic plants also demonstrate that the PR1asignal peptide, which was fused to GUS in pAG2016, did not interferedramatically with transformation efficiency or the phenotype of thetransgenic plant.

Example 19—Transgenic Plants Constructed Using pAG2014, pAG2015,pAG2020, pAG2025

The transformation vectors pAG2014, pAG2015, pAG2020, pAG2025 were usedin transformation to regenerate transgenic plants. Transformationvectors pAG2014, pAG2015, and pAG2020 were derived from pAG2005 and eachcontains an expression cassette for the production of a xylanase(accession number P77853). In pAG2014, the P77853 gene is fused to thebarley alpha amylase signal sequence (BAASS; SEQ ID NO: 8) for cell walltargeting. In pAG2015, the P77853 gene is not fused to any signalpeptide and therefore should accumulate in the cytoplasm of cells. InpAG2020, P77853 is fused to the PR1a signal peptide for targeting of theenzyme to the apoplast. In contrast, pAG2025 was derived from pAG2012,which uses the rice glutelin GluB-4 promoter and GluB-4 signal sequenceto direct seed tissue specific expression of P77853. The averagetransformation efficiency for pAG2014 was 30%, for pAG2015 it was 34%,for pAG2020 it was 24%, and for pAG2025 it was 10%. All of theseefficiencies were within the expected range of transformation efficiencywhen using the rice ubiquitin 3 promoter and PMI selection cassette.

Activity measurements were made from transgenic events generated usingmethods described above. The following figures show the results of theactivity measurements.

Referring to FIG. 22, reducing sugar measurements were made fortransgenic plants. FIG. 22 shows the reducing sugar production oftransgenic plants having pAG2014 (left sample) or pAG2004 (middlesamples), and a buffer control (right sample). Transgenic plant event #5(left sample) made with pAG2014, which expresses the P77853 xylanaseproduces significantly more reducing sugars when incubated at 60° C.than plants made with pAG2004.

Referring to FIG. 23, enzyme activity measurements were made from dried,senescent corn stover samples. The first six samples from the left inFIG. 23 are different transgenic plants having pAG2014. The seventhsample is a negative control from a transgenic plant having pAG2004.Transgenic plants made with pAG2014 were allowed to senesce, and werethen dried down in an incubator to bone dry levels. The level of dry maybe less than 1% moisture. The stover samples were milled and assayed asdescribed above. As shown, the enzyme activity is stable even throughthe senescence, drying, and milling processes. A range of activities wasobtained in this data, from low levels (close to the non-xylanaseexpressing control (2004.15)) up to over 8 μg RBB equivalents/mg stover.

Referring to FIG. 24, enzyme activity measurements were made from leaftissue samples of transgenic plants made with pAG2015, pAG2014, orpAG2004. A samples for pAG2014 is shown seventh from the right. A samplefor pAG2004 is shown last. All other samples are different transgenicevents for pAG2015 plants. As seen, a range of activity levels areobtained because gene insertion into the plant genome is highly variableand significantly affects expression properties. In general, a maximumactivity level may be achieved for a given vector, and any activitybelow that level is also possible.

As shown in FIG. 24, pAG2015 (cytoplasmic P77853) and pAG2014(BAASS:P77853) provide significant activity levels. Activity frompAG2015 is significant when expressed in plants and sampled from greentissues, and from senescent corn stover, however, thus assays have shownthat pAG2014 provides a greater level of reducing sugar production whenassayed from senescent corn stover. In contrast, pAG2025 provides noactivity in green tissues tested (data not shown in FIG. 24), as wouldbe expected given the seed specific nature of the pAG2025 transgeneexpression cassette.

FIGS. 25A and 25B illustrate transgenic plants made with pAG2014, FIG.25C illustrates a cob from a transgenic plant made with the pAG2014.FIGS. 26A and 26B illustrate transgenic plants made with pAG2015, andFIGS. 26C and 26D illustrate cobs from transgenic plants made withpAG2015. FIGS. 27A and 27B illustrate transgenic plants made withpAG2020, and FIG. 27C illustrate a cob from a transgenic plant made withpAG2020. Referring to FIGS. 28A, 28B, and 28C, transgenic plants madewith pAG2025 are illustrated. These plants demonstrate that the P77853xylanase can be effectively expressed from the expression cassettecontained within pAG2005. The transgenic plants also demonstrate thatthe BAASS [SEQ ID NO: 8] and PR1a [SEQ ID NO: 6] signal peptides, whichare fused to P77853 in pAG2014 and pAG2020, respectively, do notinterfere with transformation efficiency, but do impact phenotyperelative to cytoplasmic accumulation. The phenotypes of these plantswere very interesting and unanticipated. No known work has shown theexpression of xylanase enzymes in corn, switchgrass, sorghum, orsugarcane. Based on the results herein, xylanase enzymes impart specificphenotypes, but they were highly dependent upon the specific enzyme,signal peptide, and promoter used, as well as the presence of the ERretention signal, SEKDEL [SEQ ID NO: 12].

The P77853 xylanase is interesting because transgenic maize plants madeusing pAG2014, pAG2015, pAG2020 and pAG2025 all had normal growthphenotypes, but some had different seed phenotypes. That the plantsdevelop normally is somewhat surprising because xylanase hydrolyzesxylan in the hemicellulose component of plant cell walls

Referring to FIGS. 25A, 25B and 25C, for pAG2014 (BAASS:P77853),severely shriveled kernels were observed in many of the transgenicevents. These plants had normal growth and development, but asegregating shriveled seed phenotype was observed in multiple plants.See shriveled seeds 2510 in FIG. 25C. Shriveled seeds were randomlyselected along with normal seeds and tested for increases in xylanaseactivity (indicating presence of the P77853 enzyme). Of the seedstested, all shriveled seeds had a significant increase in xylanaseactivity, while the normal seeds had undetectable xylanase activity, asdid seeds from a wild-type plant. In addition, twelve shriveled seedswere selected from a random cob and planted alongside 12 normal lookingseeds. Of the seeds planted, only one of the 12 shriveled seedsgerminated (it was tested by PCR and shown to have the P77853 gene),while nine of the 12 normal seeds germinated. Of the nine normal seedsthat germinated, eight did not have the P77853 gene, while one did havethe P77853, as determined by PCR. This suggests that P77853, whenexpressed as a fusion with the BAASS signal sequence, results in seedsthat have reduced fertility relative to the non-transgenic seeds andthat the level of infertility may be dependent upon the level of P77853expression. While shriveled seeds and infertility would be a significantcommercial detriment in corn, it could be advantageous in switchgrass,sorghum, miscanthus, and sugarcane, where plant sterility may bebeneficial from the perspective of regulatory approval. Furthermore,perennial crops like switchgrass and sugarcane can be clonally producedvia tissue culture using methods known in the art, and vegetativelyexpanded. In these crops decreased fertility may be less of an issue andcould be advantageous for gene confinement. Thus, while the adverse seedphenotype of P77853 in corn and other grain crops is detrimental, inforage, sugar, and non-grain crops used as animal feedstocks orfermentation feedstocks, P77853 may provide significant benefits forfiber digestion, hydrolysis, and decreased fertility. Transgenicswitchgrass events made using pAG2014 were phenotypically normal.

Referring to FIGS. 26A, 26B, 26C and 26D, for pAG2015, which did nothave a signal peptide and therefore accumulated P77853 in the cytoplasmof plant cells, no adverse phenotype was observed. Some maize seeds fromthese plants were slightly more off color than the WT seeds, but noother abnormal phenotype has been observed thus far (See FIG. 26D).These plants do accumulate significant levels of xylanase activity,which is on average at least equal to, and in most events somewhathigher, than the xylanase activity detected in the pAG2014 events. Thatthe two plants do not share the same seed phenotype is noteworthy andindicates that the BAASS [SEQ ID NO: 8] signal sequence for cell walltargeting, which was used in the pAG2014 vector, contributes to the seedphenotype observed in the pAG2014 events. Because these plantsaccumulate high levels of xylanase activity, they may be useful as asource of xylanase enzyme, as a feedstock that can auto-hydrolyze thehemicellulose components for use in industrial processes such asfermentation, as a forage animal feed or animal feed additive, and as agrain animal feed or feed additive. Unlike transgenic events made usingpAG2014, those made using pAG2015 did not have an abnormal seedphenotype and may prove useful in grain crops such as corn, (grain)sorghum, wheat, barley, and others.

Referring to FIGS. 27A, 27B and 27C, for pAG2020 (PR1a:P77853) events,both the plants and cobs looked normal and did not have a significantobservable phenotype. This is particularly surprising since PR1a targetsthe fused P77853 xylanase to the apoplast, where it would be anticipatedto have an effect similar to that of the pAG2014 events. It is not knownif the PR1a signal peptide causes lower expression, lower enzymeaccumulation, or is less effective at targeting the P77853 protein, butthe absence of a seed phenotype in these transgenic plants is surprisinggiven the results obtained from pAG2014. Because these plants accumulatexylanase activity, they may be useful as a source of xylanase enzyme, asa feedstock that can auto-hydrolyze the hemicellulose components for usein industrial processes such as fermentation, as a forage animal feed oranimal feed additive, and as a grain animal feed or feed additive.Unlike transgenic events made using pAG2014, those made using pAG2020did not have an abnormal seed phenotype and may prove useful in graincrops such as corn, (grain) sorghum, wheat, barley, and others.

Referring to FIGS. 28A, 28B and 28C, For pAG2025 (GluB4:P77853) events,all plants looked phenotypically normal.

Example 20—Transgenic Plants Constructed Using pAG2017, pAG2019, andpAG2027

The transformation vectors pAG2017, pAG2019, and pAG2027 were used intransformation to regenerate transgenic plants. Transformation vectorspAG2017 and pAG2019 were derived from pAG2005, and each contains anexpression cassette for the production of a xylanase (accession numberP40942). Vector pAG2027 was derived from pAG2012 and expresses theP40942 xylanase from the GluB-4 promoter, which is expressedpredominantly in the seed. In pAG2017, the P40942 xylanase is fused tothe PR1a signal peptide for targeting of the enzyme to the apoplast. InpAG2019, the P40942 gene is fused to the barley alpha amylase signalsequence (BAASS; SEQ ID NO: 8) for cell wall targeting. The averagetransformation efficiency for pAG2017 was 16%, for pAG2019 it was 13%,and for pAG2027 it was 29%.

In contrast to transgenic plants expressing P77853, which were allphenotypically normal except for the above described seed abnormalities,plants expressing the P40942 xylanase were severely stunted, except forthose made from pAG2027. Referring to FIGS. 29A, 29B, 29C and 29D,plants transformed with pAG2017 (PR1a:P40942) are severely stunted andnever grew to the same height as the wild-type plants, or plantstransformed with pAG2020 (PR1a:P77853). FIG. 29A shows a stunted pAG2017transgenic plant. FIG. 29B shows a stunted pAG2017 transgenic plantalong side a wild type plant on the right. FIGS. 29C and 29D show cobsfrom a pAG2017 transgenic plant with partially shriveled seeds havingabnormal coloration. The results with pAG2017 were unanticipated giventhat P77853 and P40942 have approximately the same specific activitywhen measured in vitro, on birchwood xylan (see above). P40942 also hassome cellobiohydrolase (CBH) activity, so it is possible that thisactivity contributes to the observed phenotype, but other groups haveexpressed CBH enzymes in maize with apparently no growth phenotypeobserved. The significant growth phenotype difference between transgenicplants made with pAG2017 and pAG2020 is quite surprising and veryunanticipated.

In addition to the growth phenotype in the pAG2017 plants, the seedsfrom these plants, or outcrosses of these plants on to AxB non-trangenicplants, also displayed a similar shriveled phenotype as observed in theseeds of transgenic plants made with pAG2014, as well as showing somediscoloration of the seeds. Approximately 20 shriveled seeds werecollected from the pAG2017 plants and all tested positive for xylanaseactivity, while plump seeds did not have detectable increases inxylanase activity as determined using the methods described above.

Referring to FIGS. 30A and 30B, transgenic plants made with pAG2019(BAASS:P40942) also possessed a stunted growth phenotype, similar totransgenic plants made with pAG2017. This was surprising given thattransgenic plants made with pAG2014 (BAASS:P77853) did not have a growthphenotype, yet the P40942 and P77853 xylanases have approximately thesame specific activity, when measured on birchwood xylan. FIG. 30A showsa stunted transgenic plant made with pAG2019, and FIG. 30B shows a showsa stunted transgenic plant made with pAG2019 along side a wild typeplant on the left.

Referring to FIG. 31, transgenic plants made with pAG2027, which expressP40942 from the rice GlutB promoter, are phenotypically normal withregards to growth. The left three plants in FIG. 31 were made withpAG2019. The right three plants were made with pAG2027. The result withpAG2027 was in contrast to transgenic plants made with pAG2017 andpAG2019, and is surprising because P40942 expressed from the riceubiquitin promoter, using either PR1a or the BAASS signaling sequences,caused stunted growth. However, the result agrees with the observationthat plants made with pAG2025 (rice ubiquitin 3 promoter drivingP77853), are not stunted and grow normally. Given the differences inphenotypes observed between vectors expressing P77853 and P40942, itcould not be predicted what the result with pAG2027 would be. Becausethe GlutB promoter primarily expresses the enzyme in the seed, it may bethat none of the enzymes expressed from the GluB promoter will show agrowth phenotype or phenotype associated with the green tissue, and onlyseed phenotypes, similar to those observed in plants made with pAG2014and pAG2017.

Example 21—Transgenic Plants Constructed Using pAG2018 and pAG2026

The transformation vectors pAG2018 and pAG2026 were used intransformation to regenerate transgenic plants. Vector pAG2018 wasderived from pAG2005 and contains an expression cassette for theproduction of a xylanase (accession number O30700), fused to the BAASS[SEQ ID NO: 8] signal sequence. Vector pAG2026 was derived from pAG2012and expresses the O30700 xylanase from the GluB-4 promoter, which isexpressed predominantly in the seed. The average transformationefficiency for pAG2018 was 13% and for pAG2026 it was 18%.

As described above, transgenic plants expressing P77853 were allphenotypically normal except for the above described seed abnormalities.In contrast, referring to FIGS. 32A, 32B and 32C, transgenic plants madewith pAG2018 and expressing the O30700 xylanase were severely stuntedand never grew to the same height as the wild-type plants or plantstransformed with pAG2014. FIG. 32A shows two transgenic plants made withpAG2018 on the left and two non-hydrolase expressing plants on theright. FIGS. 32B and 32C each show a transgenic plant made with pAG2018.These results were unanticipated given that P77853 and O30700 are bothendo-xylanase enzymes, and in contrast to P40942, O30700 does not haveany CBH activity. The growth phenotype observed with O30700 was verysimilar to the stunted growth observed in the pAG2017 and pAG2019plants.

In contrast to transgenic plants made with pAG2018 transgenic plantsmade with pAG2026, which express O30700 from the rice GlutB promoter,are phenotypically normal with regards to growth. See FIGS. 33A, 33B and33C, which illustrate three different transgenic plants made withpAG2026. These results are surprising because O30700 expressed from therice ubiquitin promoter and fused to the BAASS [SEQ ID NO: 8] signalingsequence caused stunted growth. In contrast, the result agrees with theobservation that plants made with pAG2025 (rice ubiquitin 3 promoterdriving P77853), are not stunted and grow normally, however, given thedifferences in phenotypes observed between vectors expressing P77853 andO30700, it could not be predicted what this result would be. Because theGlutB promoter primarily expresses the enzyme in the seed, it may bethat none of the enzymes expressed from the GluB promoter will show agrowth phenotype or phenotype associated with the green tissue, and onlyseed phenotypes similar to those observed in plants made with pAG2014and pAG2017.

Example 22—Transgenic Plants Constructed Using pAG2021, pAG2023(P77853m3), pAG2022, pAG2024

The transformation vectors pAG2021, pAG2023, pAG2022, and pAG2024 wereused in transformation to regenerate transgenic plants. These vectorswere all derived from pAG2005 and contain an expression cassette for theproduction of an intein-modified xylanase (referred to as P77853m3). Intransformation vectors pAG2021 and pAG2022, the intein-modified P77853m3protein was fused to the PR1a signal peptide, while in pAG2023 andpAG2024, P77853m3 was fused to the BAASS signal peptide [SEQ ID NO: 8].Vectors pAG2022 and pAG2024 also have a SEKDEL endoplasmic reticulumretention sequence [SEQ ID NO: 12] appended to the P77853m3, whereaspAG2021 and pAG2023 lack the SEKDEL sequence [SEQ ID NO:12]. The averagetransformation efficiency for pAG2021 was 19%, for pAG2022 it was 21%,for pAG2023 it was 24%, and for pAG2024 it was 38%.

None of the transgenic plants made with pAG2021, pAG2022, pAG2023, andpAG2024 have an abnormal phenotype. See FIGS. 34A, 34B, 34C and 34D forpAG2021 results. Transgenic plants made with pAG2021 grew normally,achieved normal height and had a normal seed set. See FIGS. 35A, 35B and35C for pAG2022 results. Transgenic plants made with pAG2022 also grewnormally, achieved normal height and had a normal seed set. See FIGS.36A, 36B and 36C for pAG2023 results. These figures show that transgenicplants made with pAG2023 grew normally and achieved normal heights. SeeFIGS. 37A, 37B and 37C for pAG2024 results. These figures show thattransgenic plants made with pAG2024 also grew normally and achievednormal heights. It is demonstrated herein that intein modification of acell wall degrading enzyme can protect a plant from any phenotype thatmay be imparted by non-intein-modified enzyme. The cis-splicing intein(mini-Psp-pol M1L4 m3), designed to have temperature sensitive splicingactivity, was used in this example. Because the plants were grown atnon-splicing temperatures there was no observed activity and noassociated growth or seed phenotypes. At some temperatures the inteinmay splice to some extent and reveal active enzyme. Because the plantshave a normal phenotype, expression of intein-modified proteins is a wayof providing an embedded cell wall degrading activity in plants that canbe regained subsequently, but does not have a phenotypic effect on theplant.

Referring to FIG. 38, enzyme activity of selected transgenic events wasassayed. This figure highlights activity data from some of the pAG2021events, along with measurements from pAG2004 events (negative controlsfor xylanase activity) and a pAG20014 event (positive control forxylanase activity). For this assay, samples of dried corn stover fromsenescent plants were assayed using the methods described above. Plantsamples were labeled according to the vector number that was used tomake them. Measurements for 2014.5 (transgenic maize event made withpAG2014 and labeled at 2014.5) represent the positive control forxylanase activity, while measurements for 2004.# (transgenic maizeevents made with pAG2004) represent xylanase negative control stover. Asshown, two of the transgenic plants made with pAG2021 providesignificant amounts of xylanase activity, but the plants werephenotypically normal, unlike the pAG2014 events, which showed a seedphenotype.

Embodiments herein include but are not limited to the plants describedabove and/or illustrated in the drawings or parts thereof, vectorsencoding any amino acid sequence herein, vectors including any nucleicacid sequence herein, any amino acid sequence herein, any nucleic acidherein, any plant including a vector herein, any plant including anucleic acid herein, any plant including an amino acid sequence herein,and any method of using any plant, plant part, vector, amino acidsequence or protein sequence herein.

The sequence of pAG2015 is:

(SEQ ID NO: 207) aattcatactaaagcttgcatgcctgcaggtcgactctagtaacggccgccagtgtgctggaattaattcggcttgtcgaccacccaaccccatatcgacagaggatgtgaagaacaggtaaatcacgcagaagaacccatctctgatagcagctatcgattagaacaacgaatccatattgggtccgtgggaaatacttactgcacaggaagggggcgatctgacgaggccccgccaccggcctcgacccgaggccgaggccgacgaagcgccggcgagtacggcgccgcggcggcctctgcccgtgccctctgcgcgtgggagggagaggccgcggtggtgggggcgcgcgcgcgcgcgcgcgcagctggtgcggcggcgcgggggtcagccgccgagccggcggcgacggaggagcagggcggcgtggacgcgaacttccgatcggttggtcagagtgcgcgagttgggcttagccaattaggtctcaacaatctattgggccgtaaaattcatgggccctggtttgtctaggcccaatatcccgttcatttcagcccacaaatatttccccagaggattattaaggcccacacgcagcttatagcagatcaagtacgatgtttcctgatcgttggatcggaaacgtacggtcttgatcaggcatgccgacttcgtcaaagagaggcggcatgacctgacgcggagttggttccgggcaccgtctggatggtcgtaccgggaccggacacgtgtcgcgcctccaactacatggacacgtgtggtgctgccattgggccgtacgcgtggcggtgaccgcaccggatgctgcctcgcaccgccttgcccacgctttatatagagaggttttctctccattaatcgcatagcgagtcgaatcgaccgaaggggagggggagcgaagctttgcgttctctaatcgcctcgtcaaggtaactaatcaatcacctcgtcctaatcctcgaatctctcgtggtgcccgtctaatctcgcgattttgatgctcgtggtggaaagcgtaggaggatcccgtgcgagttagtctcaatctctcagggtttcgtgcgattttagggtgatccacctcttaatcgagttacggtttcgtgcgattttagggtaatcctcttaatctctcattgatttagggtttcgtgagaatcgaggtagggatctgtgttatttatatcgatctaatagatggattggttttgagattgttctgtcagatggggattgtttcgatatattaccctaatgatgtgtcagatggggattgtttcgatatattaccctaatgatgtgtcagatggggattgtttcgatatattaccctaatgatggataataagagtagttcacagttatgttttgatcctgccacatagtttgagttttgtgatcagatttagttttacttatttgtgcttagttcggatgggattgttctgatattgttccaatagatgaatagctcgttaggttaaaatctttaggttgagttaggcgacacatagtttatttcctctggatttggattggaattgtgttcttagtttttttcccctggatttggattggaattgtgtggagctgggttagagaattacatctgtatcgtgtacacctacttgaactgtagagcttgggttctaaggtcaatttaatctgtattgtatctggctctttgcctagttgaactgtagtgctgatgttgtactgtgtttttttacccgttttatttgctttactcgtgcaaatcaaatctgtcagatgctagaactaggtggctttattctgtgttcttacatagatctgttgtcctgtagttacttatgtcagttttgttattatctgaagatatttttggttgttgcttgttgatgtggtgtgagctgtgagcagcgctcttatgattaatgatgctgtccaattgtagtgtagtatgatgtgattgatatgttcatctattttgagctgacagtaccgatatcgtaggatctggtgccaacttattctccagctgcttttttttacctatgttaattccaatcctttcttgcctcttccagatccagataatgcagaaactcattaactcagtgcaaaactatgcctggggcagcaaaacggcgttgactgaactttatggtatggaaaatccgtccagccagccgatggccgagctgtggatgggcgcacatccgaaaagcagttcacgagtgcagaatgccgccggagatatcgtttcactgcgtgatgtgattgagagtgataaatcgactctgctcggagaggccgttgccaaacgctttggcgaactgcctttcctgttcaaagtattatgcgcagcacagccactctccattcaggttcatccaaacaaacacaattctgaaatcggttttgccaaagaaaatgccgcaggtatcccgatggatgccgccgagcgtaactataaagatcctaaccacaagccggagctggtttttgcgctgacgcctttccttgcgatgaacgcgtttcgtgaattttccgagattgtctccctactccagccggtcgcaggtgcacatccggcgattgctcactttttacaacagcctgatgccgaacgtttaagcgaactgttcgccagcctgttgaatatgcagggtgaagaaaaatcccgcgcgctggcgattttaaaatcggccctcgatagccagcagggtgaaccgtggcaaacgattcgtttaatttctgaattttacccggaagacagcggtctgttctccccgctattgctgaatgtggtgaaattgaaccctggcgaagcgatgttcctgttcgctgaaacaccgcacgcttacctgcaaggcgtggcgctggaagtgatggcaaactccgataacgtgctgcgtgcgggtctgacgcctaaatacattgatattccggaactggttgccaatgtgaaattcgaagccaaaccggctaaccagttgttgacccagccggtgaaacaaggtgcagaactggacttcccgattccagtggatgattttgccttctcgctgcatgaccttagtgataaagaaaccaccattagccagcagagtgccgccattttgttctgcgtcgaaggcgatgcaacgttgtggaaaggttctcagcagttacagcttaaaccgggtgaatcagcgtttattgccgccaacgaatcaccggtgactgtcaaaggccacggccgtttagcgcgtgtttacaacaagctgtaagagcttactgaaaaaattaacatctcttgctaagctgggagctctagatccccgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatgttactagatcgggaattggcgagctcgaattaattcagtacattaaaaacgtccgcaatgtgttattaagttgtctaagcgtcaatttgtttacaccacaatatatcctgccaccagccagccaacagctccccgaccggcagctcggcacaaaatcaccactcgatacaggcagcccatcagtccgggacggcgtcagcgggagagccgttgtaaggcggcagactttgctcatgttaccgatgctattcggaagaacggcaactaagctgccgggtttgaaacacggatgatctcgcggagggtagcatgttgattgtaacgatgacagagcgttgctgcctgtgatcaaatatcatctccctcgcagagatccgaattatcagccttcttattcatttctcgcttaaccgtgacaggctgtcgatcttgagaactatgccgacataataggaaatcgctggataaagccgctgaggaagctgagtggcgctatttctttagaagtgaacgttgacgatcgtcgaccgtaccccgatgaattaattcggacgtacgttctgaacacagctggatacttacttgggcgattgtcatacatgacatcaacaatgtacccgtttgtgtaaccgtctcttggaggttcgtatgacactagtggttcccctcagcttgcgactagatgttgaggcctaacattttattagagagcaggctagttgcttagatacatgatcttcaggccgttatctgtcagggcaagcgaaaattggccatttatgacgaccaatgccccgcagaagctcccatctttgccgccatagacgccgcgccccccttttggggtgtagaacatccttttgccagatgtggaaaagaagttcgttgtcccattgttggcaatgacgtagtagccggcgaaagtgcgagacccatttgcgctatatataagcctacgatttccgttgcgactattgtcgtaattggatgaactattatcgtagttgctctcagagttgtcgtaatttgatggactattgtcgtaattgcttatggagttgtcgtagttgcttggagaaatgtcgtagttggatggggagtagtcatagggaagacgagcttcatccactaaaacaattggcaggtcagcaagtgcctgccccgatgccatcgcaagtacgaggcttagaaccaccttcaacagatcgcgcatagtcttccccagctctctaacgcttgagttaagccgcgccgcgaagcggcgtcggcttgaacgaattgttagacattatttgccgactaccttggtgatctcgcctttcacgtagtgaacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttgtccaagataagcctgcctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgatcgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttccctcatgatgtttaactcctgaattaagccgcgccgcgaagcggtgtcggcttgaatgaattgttaggcgtcatcctgtgctcccgagaaccagtaccagtacatcgctgtttcgttcgagacttgaggtctagttttatacgtgaacaggtcaatgccgccgagagtaaagccacattttgcgtacaaattgcaggcaggtacattgttcgtttgtgtctctaatcgtatgccaaggagctgtctgcttagtgcccactttttcgcaaattcgatgagactgtgcgcgactcctttgcctcggtgcgtgtgcgacacaacaatgtgttcgatagaggctagatcgttccatgttgagttgagttcaatcttcccgacaagctcttggtcgatgaatgcgccatagcaagcagagtcttcatcagagtcatcatccgagatgtaatccttccggtaggggctcacacttctggtagatagttcaaagccttggtcggataggtgcacatcgaacacttcacgaacaatgaaatggttctcagcatccaatgtttccgccacctgctcagggatcaccgaaatcttcatatgacgcctaacgcctggcacagcggatcgcaaacctggcgcggcttttggcacaaaaggcgtgacaggtttgcgaatccgttgctgccacttgttaacccttttgccagatttggtaactataatttatgttagaggcgaagtcttgggtaaaaactggcctaaaattgctggggatttcaggaaagtaaacatcaccttccggctcgatgtctattgtagatatatgtagtgtatctacttgatcgggggatctgctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggggggggggggggggggttccattgttcattccacggacaaaaacagagaaaggaaacgacagaggccaaaaagctcgctttcagcacctgtcgtttcctttcttttcagagggtattttaaataaaaacattaagttatgacgaagaagaacggaaacgccttaaaccggaaaattttcataaatagcgaaaacccgcgaggtcgccgccccgtaacctgtcggatcaccggaaaggacccgtaaagtgataatgattatcatctacatatcacaacgtgcgtggaggccatcaaaccacgtcaaataatcaattatgacgcaggtatcgtattaattgatctgcatcaacttaacgtaaaaacaacttcagacaatacaaatcagcgacactgaatacggggcaacctcatgtccccccccccccccccctgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaattggtcgacgatcttgctgcgttcggatattttcgtggagttcccgccacagacccggattgaaggcgagatccagcaactcgcgccagatcatcctgtgacggaactttggcgcgtgatgactggccaggacgtcggccgaaagagcgacaagcagatcacgcttttcgacagcgtcggatttgcgatcgaggatttttcggcgctgcgctacgtccgcgaccgcgttgagggatcaagccacagcagcccactcgaccttctagccgacccagacgagccaagggatctttttggaatgctgctccgtcgtcaggctttccgacgtttgggtggttgaacagaagtcattatcgcacggaatgccaagcactcccgaggggaaccctgtggttggcatgcacatacaaatggacgaacggataaaccttttcacgcccttttaaatatccgattattctaataaacgctcttttctcttaggtttacccgccaatatatcctgtcaaacactgatagtttaaactgaaggcgggaaacgacaacctgatcatgagcggagaattaagggagtcacgttatgacccccgccgatgacgcgggacaagccgttttacgtttggaactgacagaaccgcaacgttgaaggagccactcagcttaattaagtctaactcgagttactggtacgtaccaaatccatggaatcaaggtaccgtcgactctagtaacggccgccagtgtgctggaattaattcggcttgtcgaccacccaaccccatatcgacagaggatgtgaagaacaggtaaatcacgcagaagaacccatctctgatagcagctatcgattagaacaacgaatccatattgggtccgtgggaaatacttactgcacaggaagggggcgatctgacgaggccccgccaccggcctcgacccgaggccgaggccgacgaagcgccggcgagtacggcgccgcggcggcctctgcccgtgccctctgcgcgtgggagggagaggccgcggtggtgggggcgcgcgcgcgcgcgcgcgcagctggtgcggcggcgcgggggtcagccgccgagccggcggcgacggaggagcagggcggcgtggacgcgaacttccgatcggttggtcagagtgcgcgagttgggcttagccaattaggtctcaacaatctattgggccgtaaaattcatgggccctggtttgtctaggcccaatatcccgttcatttcagcccacaaatatttccccagaggattattaaggcccacacgcagcttatagcagatcaagtacgatgtttcctgatcgttggatcggaaacgtacggtcttgatcaggcatgccgacttcgtcaaagagaggcggcatgacctgacgcggagttggttccgggcaccgtctggatggtcgtaccgggaccggacacgtgtcgcgcctccaactacatggacacgtgtggtgctgccattgggccgtacgcgtggcggtgaccgcaccggatgctgcctcgcaccgccttgcccacgctttatatagagaggttttctctccattaatcgcatagcgagtcgaatcgaccgaaggggagggggagcgaagctttgcgttctctaatcgcctcgtcaaggtaactaatcaatcacctcgtcctaatcctcgaatctctcgtggtgcccgtctaatctcgcgattttgatgctcgtggtggaaagcgtaggaggatcccgtgcgagttagtctcaatctctcagggtttcgtgcgattttagggtgatccacctcttaatcgagttacggtttcgtgcgattttagggtaatcctcttaatctctcattgatttagggtttcgtgagaatcgaggtagggatctgtgttatttatatcgatctaatagatggattggttttgagattgttctgtcagatggggattgtttcgatatattaccctaatgatgtgtcagatggggattgtttcgatatattaccctaatgatgtgtcagatggggattgtttcgatatattaccctaatgatggataataagagtagttcacagttatgttttgatcctgccacatagtttgagttttgtgatcagatttagttttacttatttgtgcttagttcggatgggattgttctgatattgttccaatagatgaatagctcgttaggttaaaatctttaggttgagttaggcgacacatagtttatttcctctggatttggattggaattgtgttcttagtttttttcccctggatttggattggaattgtgtggagctgggttagagaattacatctgtatcgtgtacacctacttgaactgtagagcttgggttctaaggtcaatttaatctgtattgtatctggctattgcctagttgaactgtagtgctgatgttgtactgtgatttttacccgttttatttgctttactcgtgcaaatcaaatctgtcagatgctagaactaggtggctttattctgtgttcttacatagatctgttgtcctgtagttacttatgtcagttttgttattatctgaagatatttttggttgttgcttgttgatgtggtgtgagctgtgagcagcgctcttatgattaatgatgctgtccaattgtagtgtagtatgatgtgattgatatgttcatctattttgagctgacagtaccgatatcgtaggatctggtgccaacttattctccagctgcttttttttacctatgttaattccaatcctttcttgcctcttccagatccagataatgcaaacaagcattactctgacatccaacgcatccggtacgtttgacggttactattacgaactctggaaggatactggcaatacaacaatgacggtctacactcaaggtcgcttttcctgccagtggtcgaacatcaataacgcgttgtttaggaccgggaagaaatacaaccagaattggcagtctcttggcacaatccggatcacgtactctgcgacttacaacccaaacgggaactcctacttgtgtatctatggctggtctaccaacccattggtcgagttctacatcgttgagtcctgggggaactggagaccgcctggtgccacgtccctgggccaagtgacaatcgatggcgggacctacgacatctataggacgacacgcgtcaaccagccttccattgtggggacagccacgttcgatcagtactggagcgtgcgcacctctaagcggacttcaggaacagtgaccgtgaccgatcacttccgcgcctgggcgaaccggggcctgaacctcggcacaatagaccaaattacattgtgcgtggagggttaccaaagctctggatcagccaacatcacccagaacaccttctctcagggctcttcttccggcagttcgggtggctcatccggctccacaacgactactcgcatcgagtgtgagaacatgtccttgtccggaccctacgttagcaggatcaccaatccctttaatggtattgcgctgtacgccaacggagacacagcccgcgctaccgttaacttccccgcaagtcgcaactacaatttccgcctgcggggttgcggcaacaacaataatcttgcccgtgtggacctgaggatcgacggacggaccgtcgggaccttttattaccagggcacatacccctgggaggccccaattgacaatgtttatgtcagtgcggggagtcatacagtcgaaatcactgttactgcggataacggcacatgggacgtgtatgccgactacctggtgatacagtgacctaggtccccgaatttccccgatcgttcaaacatttggcaataaagtttcttaagattgaatcctgttgccggtcttgcgatgattatcatataatttctgttgaattacgttaagcatgtaataattaacatgtaatgcatgacgttatttatgagatgggtttttatgattagagtcccgcaattatacatttaatacgcgatagaaaacaaaatatagcgcgcaaactaggataaattatcgcgcgcggtgtcatctatg ttactagatcgggaattgg.

The references cited throughout this application, are incorporated forall purposes apparent herein and in the references themselves as if eachreference was fully set forth. For the sake of presentation, specificones of these references are cited at one or more particular locationherein. A citation of a reference at a particular location indicates amanner(s) in which the teachings of the reference are incorporated.However, a citation of a reference at a particular location does notlimit the manner in which all of the teachings of the cited referenceare incorporated for all purposes.

It is understood, therefore, that this invention is not limited to theparticular embodiments disclosed, but is intended to cover allmodifications which are within the spirit and scope of the invention asdefined by the appended claims; the above description; and/or shown inthe attached drawings.

What is claimed is:
 1. A transgenic plant comprising a nucleic acid thatencodes a xylanase, wherein the transgenic plant is switchgrass, and thexylanase comprises consists of the amino acid of SEQ ID NO:
 44. 2. Thetransgenic plant of claim 1, wherein the xylanase is expressed in a seedof the switchgrass.
 3. The transgenic plant of claim 1, wherein thexylanase is expressed in a compartment of a plant cell of theswitchgrass.
 4. A method of processing plant biomass comprising: mixinga plant or part thereof with liquid to form a mixture having a liquid tosolid ratio selected from a value from 8 to 10, and maintaining themixture at a temperature less than or equal to 100° C., wherein theliquid comprises water, ammonium bisulfite and ammonium carbonate; andadding at least one cell wall degrading enzyme to the mixture, whereinthe plant is the transgenic plant of claim
 1. 5. The method of claim 4further comprising heating the mixture to maintain a temperature of 40°C. to 90° C.
 6. The method of claim 4, wherein the ammonium bisulfite isat a concentration of 8% to 38% on a wt./wt. basis with the plant orpart thereof.
 7. The method of claim 4, wherein the liquid is at a pH of7.6 to 8.5 and the ammonium carbonate is at a concentration of 4% to 19%on a wt./wt. basis with the plant or part thereof.
 8. The method ofclaim 4, wherein the at least one cell wall degrading enzyme is selectedfrom the group consisting of: an endoglucanase, a 6-glucosidase, acellobiohydrolase, a xylanase, a cellulase, a glucosidase, a xylosidase,an arabinofuronosidase and a ferulic acid esterase.
 9. The method ofclaim 8, wherein the at least one cell wall degrading enzyme comprisesat least one of an endoglucanase, a B-glucosidase and acellobiohydrolase.
 10. The method of claim 8, wherein the at least onecell wall degrading enzyme comprises a xylanase.